Shanxi Academy of Agriculture science

Taiyuan, China

Shanxi Academy of Agriculture science

Taiyuan, China
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Zhu J.-Y.,Carnegie Institution for Science | Li Y.,Carnegie Institution for Science | Cao D.-M.,CAS Institute of Botany | Cao D.-M.,Shanxi Academy of Agriculture science | And 9 more authors.
Molecular Cell | Year: 2017

The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation. © 2017 Elsevier Inc.


PubMed | Chinese Academy of Forestry, Shanxi Academy of Agriculture Science and China Agricultural University
Type: Journal Article | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016

The nearly complete mitochondrial genome (mitogenome) of the butterfly, Polyura schreiber, was determined. The sequenced mitogenome is a typical circular DNA molecule of 15320bp, containing 13 protein-coding genes, two rRNA genes, 21 tRNA genes, and a putative control region. tRNA(Phe) was failed to sequence, which was presumed to be located between tRNA(Glu) and ND5. Protein-coding genes all initiate with ATN codons and terminate with TAA codons except for COII and ND5 use a single T residue as the termination codon. All tRNAs have the clover-leaf structure except for the tRNA(Ser(AGN)) and the length of them range from 65 to 71bp. The control region is 412bp long with an A+T content of 90.5%. Our phylogenetic analysis recovered the sister-group relationship between Charaxinae and Satyrinae.


PubMed | Chinese Academy of Forestry, Shanxi Academy of Agriculture Science and China Agricultural University
Type: Journal Article | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016

The complete mitochondrial genome (mitogenome) of the butterfly, Euripus nyctelius, was determined in the present study. The mitogenome is a typical circular DNA molecule of 15,417bp, containing 37 genes and a putative control region. Thirteen protein-coding genes all initiate with ATN codons and mostly terminate with TAA or TAG codons except for COII, ND4 and ND5 use a single T residue as the termination codon. All tRNAs have the classic clover-leaf structure, except that the dihydrouridine (DHU) arm of tRNA(Ser(AGN)) forms a simple loop. Both maximum likelihood and Bayesian analyses support the monophyly of butterflies and recover high supports for the following family level relationships: (Papilionidae+(Hesperioidea+(Pieridae (Lycaenidae+Nymphalidae)))). Euripus nycteliusis is placed as sister to the genus Sasakia within Nymphalidae.


Wang J.P.,Shanxi Academy of Agriculture Science | Cao T.W.,Shanxi Academy of Agriculture Science | Xuan S.B.,Shanxi Academy of Agriculture Science | Wang H.,The Academy of Management | And 2 more authors.
Gene | Year: 2013

Sasakia funebris, a member of the lepidopteran family, Nymphalidae (superfamily Papilionoidea) is a rare species and is found only in some areas of South China. In this study, the 15,233. bp long complete mitochondrial genome of S. funebris was determined, and harbors the gene arrangement identical to all other sequenced lepidopteran insects. The nucleotide composition of the genome is highly A. +. T biased, accounting for 81.2%. All protein-coding genes (PCGs) start with typical ATN codons, except for COI which begins with the CGA codon. All tRNAs have a typical clover-leaf secondary structure, except for tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. The S. funebris A. +. T-rich region of 370. bp contains several features common to the Lepidoptera insects, including the motif ATAGA followed by a 19. bp poly-T stretch, and two tandem repeats consisting of 18. bp repeat units and 14. bp repeat units. The phylogenetic analyses of Apaturinae based on mitogenome sequences showed: (S. funebris+. Sasakia charonda). +. (Apatura metis+. Apatura ilia). This result is consistent with the morphological classification. © 2013 Elsevier B.V.


Qin G.,Shanxi Academy of Agriculture Science | Wei Y.,Shanxi University | Kang H.,Shanxi University | Dong C.,Shanxi University
Analytical Methods | Year: 2012

Based on paper substrate room temperature phosphorescence, the chiral discrimination of quinine and quinidine was achieved using Whatman No. 40 filter paper as the substrate and Pb(Ac)2 as the heavy atom perturber. The phosphorescence lifetime differences between quinine and quinidine increased from 4.45% to 27.90% upon addition of (+)-tartaric acid as the chiral modifier. This provided a rapid and simple method for the determination of the enantiomeric composition of quinine and quinidine. © 2012 The Royal Society of Chemistry.


Sasakia funebris, a member of the lepidopteran family, Nymphalidae (superfamily Papilionoidea) is a rare species and is found only in some areas of South China. In this study, the 15,233 bp long complete mitochondrial genome of S. funebris was determined, and harbors the gene arrangement identical to all other sequenced lepidopteran insects. The nucleotide composition of the genome is highly A+T biased, accounting for 81.2%. All protein-coding genes (PCGs) start with typical ATN codons, except for COI which begins with the CGA codon. All tRNAs have a typical clover-leaf secondary structure, except for tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. The S. funebris A+T-rich region of 370 bp contains several features common to the Lepidoptera insects, including the motif ATAGA followed by a 19 bp poly-T stretch, and two tandem repeats consisting of 18 bp repeat units and 14 bp repeat units. The phylogenetic analyses of Apaturinae based on mitogenome sequences showed: (S. funebris+Sasakia charonda)+(Apatura metis+Apatura ilia). This result is consistent with the morphological classification. Copyright © 2013 Elsevier B.V. All rights reserved.


Yang Z.,Shanxi Agricultural University | Yang W.,University of Science and Technology of China | Li S.,Shanxi Agricultural University | Hao J.,Shanxi Agricultural University | And 4 more authors.
PLoS ONE | Year: 2016

As the major crops in north China, spring crops are usually planted from April through May every spring and harvested in fall. Wheat is also a very common crop traditionally planted in fall or spring and harvested in summeryearbyyear. This continuous cropping system exhibited the disadvantages of reducing the fertility of soil through decreasing microbial diversity. Thus, management of microbial diversity in the rhizosphere plays a vital role in sustainable crop production. In this study, ten common spring crops in north China were chosen sole-cropped and four were chosen intercropped with peanut in wheat fields after harvest. Denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of one 16S rDNA fragment were used to analyze the bacterial diversity and species identification. DGGE profiles showed the bacterial community diversity in rhizosphere soil samples varied among various crops under different cropping systems, more diverse under intercropping system than under sole-cropping. Some intercropping-specific bands in DGGE profiles suggested that several bacterial species were stimulated by intercropping systems specifically. Furthermore, the identification of these dominant and functional bacteria by DNA sequencing indicated that intercropping systems are more beneficial to improve soil fertility. Compared to intercropping systems, we also observed changes in microbial community of rhizosphere soil under sole-crops. The rhizosphere bacterial community structure in spring crops showed a strong crop species-specific pattern. More importantly, Empedobacter brevis, a typical plant pathogen, was only found in the carrot rhizosphere, suggesting carrot should be sown prudently. In conclusion, our study demonstrated that crop species and cropping systems had significant effects on bacterial community diversity in the rhizosphere soils. We strongly suggest sorghum, glutinous millet and buckwheat could be taken into account as intercropping crops with peanut; while hulled oat, mung bean or foxtail millet could be considered for sowing in wheat fields after harvest in North China. © 2016 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Wei Y.,Shanxi University | Qin G.,Shanxi Academy of Agriculture Science | Wang W.,Shanxi University | Bian W.,Shanxi University | And 2 more authors.
Journal of Luminescence | Year: 2011

In this paper, 4-dimethylamino 2,5-dihydroxy chalcone (DMADHC), which exhibits excited state intramolecular charge transfer (ICT) characteristics, was synthesized and characterized. A sensitive optochemical sensor for Fe 3 ion was developed using DMADHC as fluorescence receptor. The fluorescence of DMADHC was gradually quenched with the addition of Fe 3 ion, which attributed to the formation of 1:1 complex between DMADHC and Fe3 ion. The sensor exhibited excellent selectivity for Fe3 ion over a large number of cation ions such as alkali, alkaline earth and transitional metal ions with a linear range of 3.984×10 -71.135×10-5 and a limit of detection of 8.223×10-8 mol/L. On this basis, the sensor was preliminary applied to the determination of the content of iron ions in multi-vitamin tablet with satisfied results and the recoveries were in the 95100% interval, and precision (n=5) was better than 5%. © 2011 Elsevier B.V.


Xiong Q.,Shanxi University | Xie Y.,Shanxi University | Zhu Y.,Shanxi University | Xue J.,Shanxi University | And 2 more authors.
Micron | Year: 2013

The aim of this study was to better understand the pathogenesis of the entomopathogenic fungus Beauveria bassiana (Balsamo) strain TST05 observed on the peach fruit moth (Carposina sasakii (Matsumura)), an important orchard pest. The morphological and ultrastructural characterization of the mature larvae of C. sasakii infected by B. bassiana was investigated by using light, scanning and transmission electron microscopy. The results of the study show that B. bassiana TST05 infected the host larvae mainly by penetrating the integument. The conidia of the fungus adhere easily to the area around the mouthparts and to the basal area around the acanthae on the thorax and abdomen. Observations of the host's defensive response to the fungal attack indicated that dark spots appeared on the cuticle and that melanization appeared in the hemocoel. After overcoming the host's defense system, the pathogen grew and reproduced primarily in the hemocoel. The infection spread sequentially to the internal tissues, e.g., fat body, muscle, Malpighian tubules, gut and even the silk gland. Ultimately, the larval internal organs and tissues were damaged very extensively. Finally, the fungus emerged through the cuticle of the dead insect and released conidiophores that could act as new pathogens to infect other larvae. © 2012 Elsevier Ltd.


PubMed | Shanxi Academy of Agriculture Science
Type: Journal Article | Journal: Gene | Year: 2013

Sasakia funebris, a member of the lepidopteran family, Nymphalidae (superfamily Papilionoidea) is a rare species and is found only in some areas of South China. In this study, the 15,233 bp long complete mitochondrial genome of S. funebris was determined, and harbors the gene arrangement identical to all other sequenced lepidopteran insects. The nucleotide composition of the genome is highly A+T biased, accounting for 81.2%. All protein-coding genes (PCGs) start with typical ATN codons, except for COI which begins with the CGA codon. All tRNAs have a typical clover-leaf secondary structure, except for tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. The S. funebris A+T-rich region of 370 bp contains several features common to the Lepidoptera insects, including the motif ATAGA followed by a 19 bp poly-T stretch, and two tandem repeats consisting of 18 bp repeat units and 14 bp repeat units. The phylogenetic analyses of Apaturinae based on mitogenome sequences showed: (S. funebris+Sasakia charonda)+(Apatura metis+Apatura ilia). This result is consistent with the morphological classification.

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