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Shantou, China

Li L.,Shantou University | Fu Y.-C.,Shantou University | Xu J.-J.,Shantou University | Lin X.-H.,Shantou Central Hospital | And 3 more authors.
Reproductive Sciences | Year: 2015

Caloric restriction (CR) is known to increase the number of primordial follicles and prolong the reproductive life span. However, how CR modulates follicular development is not well understood. In the present study, we examined the effects of CR on follicular development in rats and investigated the underlying mechanism. After 10 weeks of CR or high-fat diet, ovarian follicles at different developmental stages were examined by histological analysis. Plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estrogen (ESG) were measured, and the levels of mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6K), and phosphorylated p70S6K in the ovary were detected by Western blot. The results showed that the reserve of follicle pool in CR rats was increased, accompanied by decreased level of phosphorylated p70S6K in the ovary, and decreased serum LH, FSH, and ESG levels. Taken together, these results suggest that CR may suppress ovarian follicular development and enhance the follicle pool reserve by inhibiting mTOR signaling. © The Author(s) 2014. Source

Zhang Q.-C.,Shantou Central Hospital
Chinese Critical Care Medicine | Year: 2013

Objective: To observe the therapeutic effect of early continuous blood purification (CBP) on lactic acidosis patients. Methods: Using prospective randomized study method, 41 patients with lactic acidosis in intensive care unit (ICU) from January 2010 to April 2012 were randomly divided into CBP group (n=21) and control group (n=20). Among them, blood gas analysis, lactic acid, blood biochemistry were prospectively monitored at the time before treatment, 12, 24 and 72 hours after treatment. They were also evaluated with acute physiology and chronic health evaluation II (APACHE II) score, and length of stay in ICU and mortality in 28 days were recorded. Results: Lactic acid level and APACHE II score were gradually decreased after treatment in both groups. Compared with control group, lactic acid (mmol/L) at 12, 24 and 72 hours in CBP group was obviously lowered (12 hours: 8.23 ± 3.94 vs. 12.47 ± 4.62, 24 hours: 4.46 ± 1.57 vs. 10.54 ± 3.48, 72 hours: 2.69 ± 1.03 vs. 5.74 ± 1.56, all P<0.01), while the APACHE II score at 12, 24 and 72 hours in CBP group was also significantly lowered (12 hours: 18.23 ± 5.85 vs. 21.64 ± 5.38, 24 hours: 16.49 ± 4.62 vs. 20.61 ± 5.71, 72 hours: 11.54 ± 3.67 vs. 16.02 ± 4.34, all P<0.05). Compared with control group, length of stay in ICU (days) was also significantly shorter in CBP group (6.58 ± 3.45 vs. 11.65 ± 4.94, P<0.05), and 28-day mortality was significantly lower in CBP group (23.8% vs. 45.0%, P<0.05). Conclusion: Early correction of lactic acidosis with CBP could reduce the mortality of lactic acidosis. Source

Zhou J.,Shantou University | Peng S.-W.,Shantou University | Wang Y.-Y.,Shantou Central Hospital | Zheng S.-B.,Shantou University | And 2 more authors.
Biomaterials | Year: 2010

Tarsal repair is an important part for eyelid reconstruction. Presently traditional clinic treatments do not produce satisfactory repair effects. The key is to find a proper tarsal repair material. Microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) was studied for application as tarsal substitute in this study. PHBHHx scaffolds were implanted into tarsal defects of Sprague-Dawley rats. Eyelid samples of implanted materials and blank defect controls were collected for histological examination at weekly intervals post surgery. Results were compared among PHBHHx scaffolds, commercial acellular dermal matrices (ADM) and blank defect controls. Both PHBHHx scaffolds and ADM provided satisfactory repair results compared with the blank controls even though the implanted PHBHHx scaffolds showed a 2 weeks inflammation. Fibrous encapsulation and scaffold degradation were observed for the PHBHHx implants. Combined with its strong, elastic mechanical properties, the tissue compatible and biodegradable PHBHHx was proven to be a suitable candidate for tarsal repair. © 2010 Elsevier Ltd. Source

Zheng H.S.,Shantou Central Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2013

To explore the anatomic features of mesoesophagus in combined thoracoscopic and laparoscopic esophagectomy with three-fields lymphadenectomy. Clinical data of 67 patients undergoing thoracoscopic and laparoscopic esophagectomy with three-fields lymphadenectomy from July 2011 to September 2012 were analyzed retrospectively. All the patients underwent three-fields lymphadenectomy. Proper surgical planes were selected according to anatomy of mesoesophagus. Thoracoscopic surgical space was bounded on azygotic vein and divided into upper and low esophageal triangle. Pancreas was the key anatomical mark for laparoscopic gastric dissection, and peripancreatic space was the natural laparoscopic surgical plane. Prevertebral fascia was bottom surface of neck dissection and carotid sheath was the boundary of two sides. The median operative time was 251.6 min (range, 220 to 320 min). The median operative blood loss was 105.6 ml (range, 40 to 320 ml). The median number of lymph nodes dissected was 29.1 (range, 13 to 46, totally 1949). There was no perioperative death. Sixty-six patients were followed up with a mean follow-up time of 8.2 months (range, 2 to 14 months). Postoperative complications included reflux esophagitis in 10 and anastomotic stenosis in 3 cases. It is safe and more radical for minimally invasive esophagectomy that overall concept of minimally invasive anatomy of mesoesophagus is applied to identify the anatomic plane and landmark during operation. Source

Lu H.,Shantou Central Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To detect the level of TGF-beta, IL-10 and IL-17 in the allergic rhinitis(AR) patients pre- and post-specific immunotherapy (SIT). The level of TGF-beta, IL-10 and IL-17 in serum of 48 AR patients were detected by enzyme-linked immunosorbent assay before SIT. and the nasal symptom score (NSS) was evaluated in the first and second year after SIT. At the same time. 35 healthy persons were made as normal. (1) The level of TGF-beta and IL-10 in serum of AR patients were lower than that of normal group (P < 0.05). The level of TGF-beta and IL-10 in AR patients taken SIT for two years was lower than that of patients with one year SIT (P < 0.05), but it was higher than that of patients pre-immunotherapy (P < 0.05). However, The level of TGF-beta and IL-10 in the group that taken SIT for two years was still lower than that of normal group (P < 0.05). (2) The level of IL-17 in serum of AR patients pre- immunotherapy was higher than that of normal group (P < 0.05). The level of IL-17 continuously declined after taking SIT, However it was higher than that of normal group two years later (P < 0.05). (3) In AR patients pre-immunotherapy and post- immunotherapy for 2 years, the level of IL-10 and TGF-beta showed negative correlation with NSS respectively, the level of IL-17 was positive related to NSS respectively (P < 0.05). But the level of IL-10, TGF-beta and IL-17 showed no correlation with NSS respectively (P > 0.05) when SIT was taken for one year. IL-10 and TGF-beta were fluctuated in AR patients taking SIT, which raised first and then dropped. but the level of IL-10 and TGF-beta was higher than that of pre- immunotherapy. However, the level of IL-17 continuously declined in the whole process. It showed that the level change of IL-10, TGF-beta and IL-17 correlate with patients' symptom only two years after SIT,so the level change of IL-10, TGF-beta and IL-17 can be used as an index for evaluating the treatment effectiveness of SIT at that time. Source

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