Zhang X.-Y.,Peoples Hospital of Shangrao |
Wang W.,Shangrao Branch of Jiangxi Medical College |
Chen W.,Second Hospital of Fuzhou |
Chen J.-M.,Fuzhou General Hospital of Nanjing Military Area Command of PLA |
Xu H.,Fuzhou General Hospital of Nanjing Military Area Command of PLA
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: The oriented migration of bone marrow mesenchymal stem cells may depend on the interaction between local chemotactic factors and cell surface receptors. However, which chemotactic factors may mediate the oriented migration of bone marrow mesenchymal stem cells towards the fracture site remains unclear. OBJECTIVE: To tag autologous bone marrow mesenchymal stem cells, evaluate its role in bone healing, and detect the highly expressed factors associated with migration of bone marrow mesenchymal stem cells in the microenvironment. METHODS: The fluorescence/chimeric C57BL/6 mouse models were established, then left shankbone fracture models were also produced. The percentages of green fluorescent protein positive cells to all cells at the fracture site and the percentage of osteoblasts differentiated from bone marrow mesenchymal stem cells to all the osteoblasts were detected at different time points. The role of bone marrow mesenchymal stem cells in the fracture repairing was evaluated. The levels of chemotactic factors protein expression at the fracture site in different time points were detected with immunohistochemistry technology. RESULTS AND CONCLUSION: The percentage of green fluorescent protein positive cells to all cells at the fracture site was (3.011±0.911)%, (9.031±0.145)%, (12.064±0.145)% at 1, 5, 14 days postoperatively; and osteoblasts differentiated from bone marrow mesenchymal stem cells accounted for 50% of all the osteoblasts. After fracture, the stromal cell derived factor-1, colony stimulating factor, hepatocyte growth factor, monocyte chemoattractant protein-1, and matrix metalloproteinases-9 were expressed to varying degrees in the microenvironment, while the expression of granulocyte colony-stimulating factor was negative. The expression of stromal cell derived factor-1 in the fracture microenvironment was the highest, mainly due to the migration of bone marrow mesenchymal stem cells. Experimental findings indicate that, autologous bone marrow mesenchymal stem cells participate in and play an important role in bone healing. The stromal cell derived factor-1 plays an important role in promoting bone marrow mesenchymal stem cells migration and promoting bone healing.
Hu P.-Y.,Jiangxi University of Traditional Chinese Medicine |
Zheng Q.,Jiangxi University of Traditional Chinese Medicine |
Chen H.,Shangrao Branch of Jiangxi Medical College |
Wu Z.-F.,Jiangxi University of Traditional Chinese Medicine |
And 2 more authors.
Chinese Journal of New Drugs | Year: 2012
Objective: To investigate the in vivo pharmacokinetics and distribution of sophoridine nanoliposomes in rats. Methods: Sophoridine injection and sophoridine nanoliposome were administered by intravenous injection in rats. The plasma sophoridine concentration was measured by high-efficiency liquid chromatography (HPLC) to assess their pharmacokinetics. The concentrations of sophoridine in the heart, liver, spleen, lung and kidney were determined by HPLC, respectively. The distribution of sophoridine in tissue was analyzed. Results: The AUC of sophoridine nanoliposome was 2.42 times as much as that of sophoridine injection, meanwhile the MRT was prolonged. The concentrations of sophoridine in tissues increased initially and decreased thereafter. Compared with sophoridine injection, the average concentration of sophoridine in liver and spleen increased 4 h after intravenous injection of sophoridine liposomes, while that in other tissues decreased. Conclusion: Sophoridine liposomes can increase the distribution of sophoridine in liver and spleen indicating liver and spleen targeting properties, especially the liver targeting property.
Zhang K.-L.,Shangrao Branch of Jiangxi Medical College |
Huang X.-R.,Fujian University of Traditional Chinese Medicine |
Qi M.-X.,Fujian University of Traditional Chinese Medicine |
Guo N.,Fujian University of Traditional Chinese Medicine
International Eye Science | Year: 2012
AIM: To investigate the relative mechanisms of signal transduction of isopsoralen (ISR) and ecdysterone (ECR) on phosphorylated extracellular signal-regulated kinase (p-ERK) expression in human lens epithelial cells (HLEC), which will provide a view in better understanding the mechanisms of aged cataract, and provide the experiment bases for the prophylaxis and treatment of aged cataract. METHODS: Human lens epithelial cells (HLECs) were cultured and sub-cultured in vitro. The cultured HLECs were exposed to H2O2 at 300 μmol/L over a time course of several hours, with and without pretreatment with β-Estradiol (E2), ECR, ISR. The HLECs were used for following experiments. The changes of the expression levels of ERK phosphorylation in HLECs which exposed to H2O2, with pretreatment with certain concentration of ECR and ISR, were analyzed by flow cytometer (FCM), the signal transduction mechanism of ECR and ISR on antioxidative damage was studied. RESULTS: The expression levels of ERK phosphorylation were examined in normal HLECs by FCM, and peaked at 6 hour. The p-ERK levels of H2O2 group gradually decreased with a prolongation of treatment time. There were significant differences as compared with control group respectively(P<0.01). The p-ERK levels of E2 group gradually increased with a prolongation of treatment time, showed significant increase from 6 hour when compared with H2O2 group respectively(P<0.01), and peaked at 6 hour. The p-ERK levels of ECR group showed significant increase from 3 hour when compared with H2O2 group respectively(P<0.01), and peaked at 12 hour. The p-ERK levels of ISR group showed significant increase from 1 hour when compared with H2O2 group respectively (P<0.01), and peaked at 1 hour. CONCLUSION: The resistance against oxidant-induced injury by E2, ECR and ISR in HLECs might relate to ERK/MAPK signaling pathway.
Cheng X.,Nanchang University |
Xu G.,Nanchang University |
Xu G.,Shangrao Branch of Jiangxi Medical College
Clinical and Experimental Hypertension | Year: 2010
Many studies reported the association between aldosterone synthase gene CYP11B2 polymorphism and essential hypertension in Chinese. So far, no meta-analysis was conducted between the etiology of essential hypertension and CYP11B2-344 CT polymorphism in Han Chinese, the majority (93 of the total population) in China. Recruited literature was based on searching the Cochrane Library, MEDLINE, EMBASE, the Chinese Biomedicine Database (CBM), CNKI, VIP, and reference lists of articles without language restrictions. Nine studies with case-control involving 4259 unselected essential hypertension patients and 3213 controls were included in the analysis. From the nine homogeneous studies with gender, age, and ethnicity matched controls, we found no significant association between the etiology of essential hypertension and the-344 CT variant in Han Chinese with random effect models (for homozygous CC: odds ratio (OR), 1.04, 95 confidence interval (CI), 0.791.37, P 0.79; for allele C: OR, 1.04, 95 CI, 0.921.18, P 0.56). No significant association was observed between CYP11B2-344 CT polymorphism and hypertension susceptibility in both sexes. Current large sample analysis did not support the association between the etiology of essential hypertension and CYP11B2-344 CT polymorphism in Han Chinese. © Informa UK, Ltd.
Gao G.,Soochow University of China |
Gao G.,Shangrao Branch of Jiangxi Medical College |
Ze Y.,Soochow University of China |
Li B.,Soochow University of China |
And 12 more authors.
Journal of Hazardous Materials | Year: 2012
Although numerous studies have described the accumulation of titanium dioxide nanoparticles (TiO2 NPs) in the liver, kidneys, lung, spleen, and brain, and the corresponding damage, it is unclear whether or not TiO2 NPs can be translocated to the ovary and cause ovarian injury, thus impairing fertility. In the current study, ovarian injury and gene-expressed characteristics in female mice induced by intragastric administration of TiO2 NPs (10mg/kg) for 90 consecutive days were investigated. Our findings indicated that TiO2 NPs can accumulate in the ovary and result in ovarian damage, cause an imbalance of mineral element distribution and sex hormones, decrease fertility or the pregnancy rate and oxidative stress in mice. Microarray analysis showed that in ovaries from mice treated with TiO2 NPs compared to controls, 223 genes of known function were up-regulated, while 65 ovarian genes were down-regulated. The increased expression of Cyp17a1 following TiO2 NPs treatment suggested that the increase in estradiol biosynthesis may be a consequence of increased TiO2 NPs. In addition, the elevated expression of Akr1c18 implied that progesterone metabolism was accelerated, thus causing a decrease in the progesterone concentration. Taken together, the apparent regulation of key ovarian genes supports the hypothesis that TiO2 NPs directly affects ovarian function. © 2012.