Shanghai Zhonghua Pharmaceutical Co.

Shanghai, China

Shanghai Zhonghua Pharmaceutical Co.

Shanghai, China

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Wang T.,Shanghai University of Traditional Chinese Medicine | Ding L.,Shanghai Zhonghua Pharmaceutical Co. | Jin H.,Shanghai Zhonghua Pharmaceutical Co. | Shi R.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
Biomedical Chromatography | Year: 2016

A sensitive, specific, accurate HPLC-MS/MS method was developed and validated for the simultaneous quantification of catechin, epicatechin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, piperine and glycyrrhetinic acid from Longhu Rendan pills in rat plasma. Chromatographic separation was performed with a Hypersil Gold C18 column using a gradient of methanol and 0.01% acetic acid containing 0.2 mm ammonium acetate as mobile phase. The analytes were quantified on a triple quadrupole mass spectrometer, operating in selected reaction monitoring mode and switching the electrospray ion source polarity between positive and negative modes in a single run. The calibration curves of catechin, epicatechin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, piperine and glycyrrhetinic acid were linear over the concentration ranges of 5–2000, 5–2000, 0.5–200, 0.5–200, 0.25–100, 0.25–100, 0.025–10 and 0.50–200 ng mL−1, respectively. The intra- and inter-assay precisions and accuracies were <11.6 and 91.9–108.2%, respectively, for all analytes. Matrix effects for all analytes were between 88.2 and 114.2%. Stability testing showed that all analytes were stable in plasma at 24 °C for 3 h, at 4 °C for 24 h, after three freeze–thaw cycles, and at −80 °C for 15 days. The method was successfully applied to an in vivo study evaluating the pharmacokinetics of multiple nonvolatile compounds following intragastric administration of Longhu Rendan pills to rats. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.


Wang T.-M.,Shanghai University of Traditional Chinese Medicine | Ding L.-Q.,Shanghai Zhonghua Pharmaceutical Co. | Jia Y.-Q.,Shanghai University of Traditional Chinese Medicine | Jin H.-J.,Shanghai Zhonghua Pharmaceutical Co. | And 3 more authors.
Analytical Methods | Year: 2014

Longhu Rendan pills (LRPs) are one of the most widely used traditional Chinese over-the-counter medicines for the prevention and treatment of heat stroke and motion sickness. A rapid and effective GC-MS/MS method for the determination of six volatile active constituents including menthol, borneol, isoborneol, anethole, eugenol and acetyl eugenol in LRPs was developed and validated. The six compounds were separated within 8 min using a VF-WAXms capillary column, and the analytes were quantified using GC-MS/MS in multiple reaction monitoring mode. Good linearity was achieved (r > 0.9973). Variations in the intra- and inter-day precisions of all the analytes were below 4.32%, and the accuracy (92.44% to 103.64%) was evaluated using a recovery test. The method successfully determined six volatile compounds in three batches of LRP samples. The present study offers a highly accurate, sensitive and reliable method for the determination of six volatile active constituents in LRPs to promote the quality control investigation of LRPs. © the Partner Organisations 2014.


Wang T.-M.,Shanghai University of Traditional Chinese Medicine | Ding L.-Q.,Shanghai Zhonghua Pharmaceutical Co. | Jin J.-H.,Shanghai Zhonghua Pharmaceutical Co. | Shi R.,Shanghai University of Traditional Chinese Medicine | And 5 more authors.
Analytical Methods | Year: 2014

A novel method based on ultra-high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry was developed for the simultaneous determination of the 14 major active constituents of Longhu Rendan pills. These 14 compounds were separated within 20 min in a C18 column (2.1 mm i.d. × 100 mm, 3 μm), and good linearity was achieved (r > 0.9980). Gradient elution was applied using a mobile phase of 0.01% formic acid containing 0.2 mM ammonium formate/acetonitrile. The analytes were quantified on an LCQ ion trap mass spectrometer in electrospray ionisation full-scan mode. Variations in the intra- and inter-day precisions of all analytes were below 4.60%, and the accuracy was evaluated by a recovery test within 94.41% to 103.39%. The method successfully quantified the 14 compounds in three sample batches of Longhu Rendan pills. Therefore, our method enables the highly accurate, sensitive and reliable determination of 14 major active constituents, which can aid the quality control investigation of Longhu Rendan pills. © The Royal Society of Chemistry 2014.


Wang T.-M.,Shanghai University of Traditional Chinese Medicine | Ding L.-Q.,Shanghai Zhonghua Pharmaceutical Co. | Jin H.-J.,Shanghai Zhonghua Pharmaceutical Co. | Shi R.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
RSC Advances | Year: 2015

Longhu Rendan pills (LRPs), a traditional Chinese over-the-counter medicine, have been used for the prevention and treatment of heatstroke and motion sickness. A sensitive, specific, and accurate headspace-solid-phase dynamic extraction method coupled to gas chromatography-tandem mass spectrometry (HS-SPDE-GC-MS/MS) was developed and validated for the investigation of the pharmacokinetic properties of l-menthol, borneol, isoborneol, and the metabolite camphor in rats after oral administration of LRPs. Target compounds were extracted using an SPDE needle device coated with a polydimethylsiloxane solid phase. Detection of components was achieved by GC-MS/MS in multiple reaction monitoring mode. This method was successfully applied in the evaluation of the pharmacokinetics of components and a metabolite of LRPs after a single intragastric administration of a 0.92 g kg-1 dose to rats. Pharmacokinetic parameters were calculated from the plasma concentration-time data. Cmax values of l-menthol, borneol, isoborneol, and camphor in rat plasma were determined to be 876 ± 341, 268 ± 149, 158 ± 91, and 126 ± 56 ng mL-1, respectively, and the AUC0-t values were measured as 876 ± 259, 408 ± 121, 140 ± 50, and 401 ± 35 ng h mL-1, respectively. These results provide useful information on the effective components of LRPs. This journal is © The Royal Society of Chemistry 2015.


PubMed | Shanghai University of Traditional Chinese Medicine and Shanghai Zhonghua Pharmaceutical Co.
Type: Journal Article | Journal: Biomedical chromatography : BMC | Year: 2016

A sensitive, specific, accurate HPLC-MS/MS method was developed and validated for the simultaneous quantification of catechin, epicatechin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, piperine and glycyrrhetinic acid from Longhu Rendan pills in rat plasma. Chromatographic separation was performed with a Hypersil Gold C18 column using a gradient of methanol and 0.01% acetic acid containing 0.2mm ammonium acetate as mobile phase. The analytes were quantified on a triple quadrupole mass spectrometer, operating in selected reaction monitoring mode and switching the electrospray ion source polarity between positive and negative modes in a single run. The calibration curves of catechin, epicatechin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, piperine and glycyrrhetinic acid were linear over the concentration ranges of 5-2000, 5-2000, 0.5-200, 0.5-200, 0.25-100, 0.25-100, 0.025-10 and 0.50-200ngmL(-1) , respectively. The intra- and inter-assay precisions and accuracies were <11.6 and 91.9-108.2%, respectively, for all analytes. Matrix effects for all analytes were between 88.2 and 114.2%. Stability testing showed that all analytes were stable in plasma at 24C for 3h, at 4C for 24h, after three freeze-thaw cycles, and at -80C for 15days. The method was successfully applied to an in vivo study evaluating the pharmacokinetics of multiple nonvolatile compounds following intragastric administration of Longhu Rendan pills to rats. Copyright 2016 John Wiley & Sons, Ltd.

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