Shanghai Zeyuan Marine Biotechnology Ltd Company

Shanghai, China

Shanghai Zeyuan Marine Biotechnology Ltd Company

Shanghai, China

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Wang J.,East China University of Science and Technology | Li Y.,East China University of Science and Technology | Wang W.,East China University of Science and Technology | Huang J.,East China University of Science and Technology | And 3 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2014

Photoautotrophic cultivation with heterotrophic cells as seeds (heterotrophic cells / photoautotrophic cultivation) is an effective way for the development of microalgal biofuel, but its development potential from the point of process optimization has not been investigated in literatures. To evaluate this, the optimizations of medium and culture conditions for Chlorella ellipsoidea were studied. In the heterotrophic stage, the biomass concentration reached 11.04 g/L with the optimized medium in flask, which were 28.0% higher than that with the original medium, and the biomass concentration reached 73.89 g/L in 5-L fermenter. In the photoautotrophic stage, the culture medium and conditions were studied in a 2-L column photobioreactor. The maximum biomass concentration, lipid content and lipid productivity reached 1.62 g/L, 36.34% and 6.1 mg/(L·h) under the optimal photoautotrophic conditions. The lipids were mainly composed of C16-C18 fatty acids, which were raw material suitable for biodiesel. After optimization, heterotrophic cells / photoautotrophic cultivation can significantly improve the capacity of biofuel production by Chlorella ellipsoidea, this method is also expected to be an efficient way for the cultivation of other microalgae that can grow heterotrophically. Copyright © 2014 by the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology.


Patent
East China University of Science, Technology, Jiaxing Zeyuan Bio Products Co. and Shanghai Zeyuan Marine Biotechnology Co. | Date: 2013-09-26

The present invention relates to a novel method for producing astaxanthin by using microalgae. The method comprises: heterotrophic cultivation of microalgae, dilution, photo-induction, collection of microalgal cells, and extraction of astaxanthin. The method according to the present invention takes full advantages of rapid growth rate in the heterotrophic stage and fast accumulation of astaxanthin in the photo-induction stage by using a large amount of microalgal cells obtained in the heterotrophic cultivation stage, so as to greatly improve the astaxanthin production rate and thereby achieve low cost, high efficiency, large scale production of astaxanthin by using microalgae. The method not only provides an important technical means to address the large scale industrial production of astaxanthin through microalgae but also ensures an ample source of raw material for the widespread utilization of astaxanthin.


Patent
East China University of Science, Technology, Jiaxing Zeyuan Bio Products Co. and Shanghai Zeyuan Marine Biotechnology Co. | Date: 2015-06-03

The present invention relates to a novel method for producing astaxanthin by using microalgae. The method comprises: heterotrophic cultivation of microalgae, dilution, photo-induction, collection of microalgal cells, and extraction of astaxanthin. The method according to the present invention takes full advantages of rapid growth rate in the heterotrophic stage and fast accumulation of astaxanthin in the photo-induction stage by using a large amount of microalgal cells obtained in the heterotrophic cultivation stage, so as to greatly improve the astaxanthin production rate and thereby achieve low cost, high efficiency, large scale production of astaxanthin by using microalgae. The method not only provides an important technical means to address the large scale industrial production of astaxanthin through microalgae but also ensures an ample source of raw material for the widespread utilization of astaxanthin.


Liu Z.,East China University of Science and Technology | Liu Z.,Xinxiang Medical University | Wei H.,East China University of Science and Technology | Li Y.,East China University of Science and Technology | And 4 more authors.
Biocontrol Science and Technology | Year: 2014

Spore survival and moisture content are two important properties of biopesticides, and both are related to field biocontrol efficacy and storage shelf life. In this study, Paenibacillus polymyxa (HY96-2) was spray-dried on both pilot plant and production scales, and the effects of inlet and outlet temperatures on spore survival and moisture content were investigated. The results showed that inlet temperatures ranging from 170 to 230 °C (at an outlet temperature of 80 °C) had no obvious effect on the two properties during pilot scale processing, although an inlet temperature of 230 °C resulted in higher feed speed. When the outlet temperature on the pilot scale was reduced from 100 to 80 °C, no obvious variations in spore survival and moisture content were found, while a further reduction from 80 to 65 °C resulted in a decline in spore survival from 81.0 to 67.0% and an increase in moisture content from 2.3 to 31.7%. These results indicate that both outlet temperature and moisture content have an effect on spore survival. Optimum inlet and outlet temperatures for P. polymyxa processing were 230 °C and 85-90 °C on a production scale. Under these conditions, spore survival and moisture content were 83.5-86.6% and 2.73--4.12%, respectively. © 2014 © 2014 Taylor & Francis.


Liu Z.,East China University of Science and Technology | Wei H.,East China University of Science and Technology | Li Y.,East China University of Science and Technology | Li S.,Shanghai Zeyuan Marine Biotechnology Ltd Company | And 2 more authors.
Biocontrol Science and Technology | Year: 2011

Two milling methods and five surfactants were tested for their effects on the suspensibility of a wettable powder formulation of Paenibacillus polymyxa HY96- 2 (P. polymyxa original powder or PPOP). Both hammer milling and jet milling significantly improved suspensibility of PPOP (from 8.1 to 26.4% and 58.3%, respectively), and did not decrease spore viability. Five surfactants (EFW®, D-425®, sodium dodecyl benzene sulfonate, tea saponin and sodium lignosulphonate) were each mixed with jet milled powder (JMP). The best results were obtained with sodium lignosulphonate and D425. These gave equal improvement in suspensibility. Sodium lignosulphonate had the best biocompatibility; spore viability values of JMP mixed with this at 1, 3, 5, or 10% (w/w) were all over 95.0%. Addition of sodium lignosulphonate to hammer milled powder (HMP) or JMP at 5% (w/w) increased suspensibility from 26.4 to 38.6% and from 58.3 to 81.1%, respectively. Our results suggest that jet milling and sodium lignosulphonate can synergistically improve the suspensibility of P. polymyxa wettable powder without adverse effects on spore viability. These findings will accelerate the commercialization of P. polymyxa wettable powder and provide a basis for the development of other biopesticides. © 2011 Taylor & Francis.


Fan J.,East China University of Science and Technology | Huang J.,East China University of Science and Technology | Li Y.,East China University of Science and Technology | Han F.,East China University of Science and Technology | And 4 more authors.
Bioresource Technology | Year: 2012

A novel cultivation strategy called " sequential heterotrophy-dilution-photoinduction" was developed for efficient algal biomass and lipid production. Three . Chlorella species were first cultivated heterotrophically to achieve high cell density, then the broth was diluted to suitable concentration (2-5. g/L) and transferred to light environment for photoinduction. With this strategy, the . Chlorella intracellular protein and chlorophyll increased rapidly to 50.87% and 32.97. mg/g by a 12-h illumination, which were close to the level of cells cultivated photoautotrophically. Moreover, the lipid contents were increased by 84.57%, 70.65% and 121.59% within 24-h photoinduction for . C. vulgaris, . C. pyrenoidosa and . C. ellipsoidea, respectively. Maximum lipid content as 26.11% of biomass and maximum lipid productivity of 89.89. mg/L/d was both accomplished by . C. pyrenoidosa. Further outdoor experiments showed consistent patterns. Therefore, the proposed strategy provided an effective approach for microalgal biomass production to meet the urgent need for both health food and biodiesel. © 2012 Elsevier Ltd.

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