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LU P.,Shanghai JiaoTong University | Hu Y.-N.,Chinese Academy of Sciences | Sheng H.-G.,Shanghai Xuhui District Central Hospital
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To explore the correlation of the plasma cholesterol level and the plasma amyloid β (Aβ) level.Methods: Among patients aged 50-65 years, 16 patients with borderline high cholesterol and 16 patients with high cholesterol were randomly selected as the high cholesterol group and 16 healthy subjects were randomly selected as the control group. The levels of plasma Aβ- 40, Aβ- 42, total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), and triglyceride (TG) of two groups were detected and their correlations were analyzed.Results: The levels of plasma Aβ- 40, Aβ- 42, LDL-C, and TG of the high cholesterol group were significantly higher than those of the control group (P<0.01). The linear correlation analysis showed that for the control group, Aβ- 40 and Aβ- 42 did not correlate with TC, LDL-C, HDL-C, and TG. For the high cholesterol group, Aβ- 40 and Aβ- 42 positively correlated with TC (P<0.01 or P<0.05), and TC positively correlated with TG and LDL-C (P<0.01 or P<0.05) and negatively correlated with HDL (P<0.05). After TG, LDL-C, and HDL-C were adjusted, partial correlation analysis was performed for TC versus Aβ- 40 and Aβ- 42. The results showed that Aβ- 40 and Aβ- 42 still positively correlated with TC (P<0.05).Conclusion: For patients with high cholesterol, the levels of plasma Aβ- 40 and Aβ- 42 significantly increase and the plasma Aβ level is correlated with plasma TC level.


Sun L.,University of Chinese Academy of Sciences | Yu Y.,University of Chinese Academy of Sciences | Huang T.,University of Chinese Academy of Sciences | An P.,University of Chinese Academy of Sciences | And 11 more authors.
PLoS ONE | Year: 2012

Background: Few studies assessed effects of individual and multiple ions simultaneously on metabolic outcomes, due to methodological limitation. Methodology/Principal Findings: By combining advanced ionomics and mutual information, a quantifying measurement for mutual dependence between two random variables, we investigated associations of ion modules/networks with overweight/obesity, metabolic syndrome (MetS) and type 2 diabetes (T2DM) in 976 middle-aged Chinese men and women. Fasting plasma ions were measured by inductively coupled plasma mass spectroscopy. Significant ion modules were selected by mutual information to construct disease related ion networks. Plasma copper and phosphorus always ranked the first two among three specific ion networks associated with overweight/obesity, MetS and T2DM. Comparing the ranking of ion individually and in networks, three patterns were observed (1) "Individual ion," such as potassium and chrome, which tends to work alone; (2) "Module ion," such as iron in T2DM, which tends to act in modules/network; and (3) "Module-individual ion," such as copper in overweight/obesity, which seems to work equivalently in either way. Conclusions: In conclusion, by using the novel approach of the ionomics strategy and the information theory, we observed potential associations of ions individually or as modules/networks with metabolic disorders. Certainly, these findings need to be confirmed in future biological studies. © 2012 Sun et al.


Xu Y.-H.,Shanghai Xuhui District Central Hospital | Xu Y.-H.,Chongqing Medical University
Chinese Journal of Interventional Imaging and Therapy | Year: 2011

Based on the combination of anatomical location by MR guidance, real-time temperature mapping and high intensity focused ultrasound, non-invasive MR-guided high intensity focused ultrasound (MRgHIFU) has shown comparative advantages that superior to surgery in the treatment of some tumor and non-tumor diseases, such as ablating uterine fibroids, adenomyosis, breast tumors, bone metastasis, prostate cancer, hepatic carcinoma and cerebral tumor in clinical practice. Meanwhile, preclinical studies for functional neurosurgery and acute stroke treatment are in progress. The therapeutic principles, clinical applications and progresses of MRgHIFU were reviewed in this article.


Wang G.-Y.,Shanghai Putuo District Peoples Hospital | Xia W.,Shanghai Putuo District Peoples Hospital | Zhang Y.-N.,Shanghai Putuo District Peoples Hospital | Li Y.,Tongji University | And 3 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To explore the inhibitory effect of RNA interference (RNAi) targeting human telomerase reverse transcriptase (hTERT) gene of colon carcinoma SW480 cells. METHODS: Four small interfering RNAs (siRNAs) targeting hTERT gene were synthesized by chemistry, and siRNAs were transfected into SW480 cells by cationic liposome. And SW480 cells were divided into six groups: blank group (untransfected SW480 cells group), siRNA-NC group(negative control group), siRNA 1-4 group (RNA interference group). The hTERT mRNA, hTERT protein and telomerase activity were detected by RT-PCR, Western blot and TRAP process, respectively. The proliferation of the SW480 cell was analyzed by MTS kits. RESULTS: The hTERT mRNA relative expression of siRNA 1-4 groups after transfection were as follow: 0.54 ± 0.18, 0.56 ± 0.19, 0.46 ± 0.10, 0.84 ± 0.18. The telomerase relative activity of siRNA 1-4 groups were 0.57± 0.18, 0.56 ± 0.19, 0.09 ± 0.08, 0.70 ± 0.19, respectively. The relative expression level of hTERT protein of siRNA 1-4 groups were 0.41 ± 0.18, 0.45 ± 0.17, 0.08 ± 0.07, 0.82 ± 0.16, respectively. SW480 cells average inhibition rate were 42.14%, 39.12%, 48.05%, 35.85%, respectively. In contrast to the blank groups, the relative expression level of the hTERT mRNA and protein and telomerase activity of siRNA-3 group was declined (P< 0.05), in addition, the difference of them in siRNA 3 group vs the blank were significant (P<0.01). CONCLUSIONS: RNAi can inhibit the proliferation of the cancer cells. The hTERT may be a target point of gene therapy for Colon carcinoma.


Liu Q.,CAS Shanghai Institutes for Biological Sciences | Tao B.,CAS Shanghai Institutes for Biological Sciences | Liu G.,CAS Shanghai Institutes for Biological Sciences | Chen G.,CAS Shanghai Institutes for Biological Sciences | And 3 more authors.
Journal of Biological Chemistry | Year: 2016

Multiple myeloma (MM) is a plasma cell malignancy withouteffective therapeutics. Thromboxane A2 (TxA2)/TxA2 receptor(T prostanoid receptor (TP)) modulates the progression of somecarcinomas; however, its effects on MM cell roliferationremain unclear. In this study, we evaluated cyclooxygenase(COX) enzymes and downstream prostaglandin profiles inhuman myeloma cell lines RPMI-8226 and U-266 and analyzedthe effects of COX-1/-2 inhibitors SC-560 and NS-398 on MMcell proliferation. Our observations implicate COX-2 as beinginvolved in modulating cell proliferation. We further incubatedMM cells with prostaglandin receptor antagonists or agonistsand found that only the TP antagonist, SQ29548, suppressedMMcell proliferation. TP silencing and the TP agonist, U46619, further confirmed this finding. Moreover, SQ29548 and TPsilencng promotedMMcell G2/M phase delay accompanied byreducing cyclin B1/cyclin-dependent kinase-1 (CDK1) mRNAand protein expression. Notably, cyclin B1 overexpression rescuedMM cells from G2/M arrest. We also found that the TPagonist activated JNK and p38 MAPK phosphorylation, and inhibitors of JNK and p38 MAPK depressed U46619-inducedproliferation and cyclin B1/CDK1 protein expression. In addition, SQ29548 and TP silencing led to the MM cell apoptoticrate increasing with improving caspase 3 activity. The knockdownof caspase 3 reversed the apoptotic rate. Taken together,our results suggest that TxA2/TP promotes MM cell proliferationby rducing cell delay at G2M phase via elevating p38MAPK/JNK-mediated cyclin B1/CDK1 expression and hinderingcell apoptosis. The TP inhibitor has potential as a novelagent to target kinase cascades forMMtherapy. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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