Shanghai Xuhui Dahua Hospital

Shanghai, China

Shanghai Xuhui Dahua Hospital

Shanghai, China
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Li F.,Shanghai Xuhui Dahua Hospital | Wu W.,Shanghai JiaoTong University | Xiang L.,Shanghai Xuhui Dahua Hospital | Weng G.,Shanghai Xuhui Dahua Hospital | And 3 more authors.
International Journal of Nanomedicine | Year: 2015

Nanoporous magnesium–zinc–silicon (n-MZS) xerogels with a pore size of ~4 nm, a surface area of 718 cm2/g, and a pore volume of 1.24 cm3/g were synthesized by a sol–gel method. The n-MZS xerogels had high capacity to load vancomycin hydrochloride (VH) and human bone morphogenetic protein-2 (rhBMP-2), after soaking in phosphate buffered saline (PBS) for 24 hours (1.5 and 0.8 mg/g, respectively). Moreover, the n-MZS xerogels exhibited the sustained release of VH and rhBMP-2 as compared with magnesium–zinc–silicon (MZS) xerogels without nanopores (showing a burst release). The VH/rhBMP-2/n-MZS system not only exhibited a good antibacterial property but also promoted the MG63 cell proliferation and differentiation demonstrating good bactericidal activity and cytocompatibility. The results suggested that n-MZS with larger surface area and high pore volume might be a promising carrier for loading and sustained release of VH and rhBMP-2. Hence, the VH/rhBMP-2/n-MZS system might be one of the promising biomaterials for osteomyelitis treatment and bone repair. © 2015 Li et al.

Shan X.,Shanghai Institute of Technology | Liu C.,East China University of Science and Technology | Li F.,Shanghai Xuhui Dahua Hospital | Ouyang C.,Shanghai Institute of Technology | And 2 more authors.
Designed Monomers and Polymers | Year: 2015

In this study, the different encapsulation methods involving emulsification and coaxial electrospinning were both utilized to fabricate a series of core/sheath composite, nanoparticles (NPs) and Nanofibers (NFs) separately, for drug delivery on potential biological and therapeutic applications. Bovine serum albumin (BSA) was employed as an active pharmaceutical ingredient model for core; poly(L-lactic acid) (PLLA) and methoxy poly(ethyleneglycol)-Poly lactic acid (mPEG-PLA) were selected as the encapsulation matrix for sheath. Attributed to the optimized fabrication procedures, the obtained NPs and NFs had the small average diameters and narrow size distributions with uniform structures and smooth surface morphologies. Based on the drug release profiles, both the NPs provided a burst release process followed by a drug diffusion manner, while for the NFs, the drug diffusion was the predominant factor in drug release. In particular, the mPEG-PLA NFs were fabricated with excellent hydrophilicity and highly neutralized surface resulting in a sustained release of BSA over 10 days. In addition, mPEG-PLA NFs also provided a better zero-order drug release profiles during the release time from 8 to 72 h, and a one-dimensional Fickian diffusion pattern during the whole BSA release period. A cytotoxicity study suggested that the two drug delivery systems were both safe to cells. In conclusions, the synergism of PEGylation with coaxial electrospinning may be an effective way to retard the release of drugs in a more sustained manner. © 2015 © 2015 Taylor & Francis.

Shan X.,Shanghai Institute of Technology | Li F.,Shanghai Xuhui Dahua Hospital | Liu C.,East China University of Science and Technology | Gao Q.,Shanghai Institute of Technology
Journal of Applied Polymer Science | Year: 2014

To improve the electrospinnability of chitosan (CS), a series of nanofiber membrane blends comprised of CS, poly(lactic acid) (PLA), and nonionic surfactant polyoxyethylene nonylphenol ether (TX-15), were made. Uniform nanofibers with no bead-like structures were obtained from solutions of 2% TX-15 with 6% CS(50)/PLA(50). The diameter was between 200 and 300 nm. We found that with increasing TX-15 in the blend, the nanofibers displayed more hydrophilicity. Compared to CS/PLA nanofibers, the blend polymers with TX-15 had better tensile mechanical properties. Finally, all cells examined showed high levels of attachment and spreading on CS/PLA/TX-15 nanofibers with a TX-15 content of 0 3%. Thus, the nanofibers were nontoxic. In conclusion, adding PLA and TX-15 to CS via solution-blending and electrospinning may be an effective way to toughen CS nanofibers and make them more suitable for drug delivery or tissue engineering applications. © 2014 Wiley Periodicals, Inc.

Tian Z.,Fudan University | Yu Q.,Fudan University | Xie Y.,Fudan University | Li F.,Shanghai Xuhui Dahua Hospital | And 6 more authors.
Pharmaceutical Research | Year: 2016

Purpose: To achieve controlled release of integral nanoparticles by the osmotic pump strategy using nanostructured lipid carriers (NLCs) as model nanoparticles. Methods: NLCs was prepared by a hot-homogenization method, transformed into powder by lyophilization, and formulated into osmotic pump tablets (OPTs). Release of integral NLCs was visualized by live imaging after labeling with a water-quenching fluorescent probe. Effects of formulation variables on in vitro release characteristics were evaluated by measuring the model drug fenofibrate. Pharmacokinetics were studied in beagle dogs using the core tablet and a micronized fenofibrate formulation as references. Results: NLCs are released through the release orifices of the OPTs as integral nanoparticles. Near zero-order kinetics can be achieved by optimizing the influencing variables. After oral administration, decreased Cmax and steady drug levels for as long as over 24 h are observed. NLC-OPTs show an oral bioavailability of the model drug fenofibrate similar to that of the core tablets, which is about 1.75 folds that of a fast-release formulation. Conclusion: Controlled release of integral NLCs is achieved by the osmotic pump strategy. © 2016 Springer Science+Business Media New York

PubMed | Fudan University and Shanghai Xuhui Dahua Hospital
Type: Journal Article | Journal: Drug delivery | Year: 2016

The main purpose of this study was to improve the oral bioavailability of sirolimus (SRL), a poorly water-soluble immunosuppressant, by encapsulating into lipids-based nanostructured lipid carriers (NLCs). SRL-loaded NLCs (SRL-NLCs) were prepared by a high-pressure homogenization method with glycerol distearates (PRECIROL ATO-5) as the solid lipid, oleic acid as the liquid lipids, and Tween 80 as the emulsifier. The SRL-NLCs prepared under optimum conditions was spherical in shape with a mean particle size of about 108.3nm and an entrapment efficiency of 99.81%. In vitro release of SRL-NLCs was very slow, about 2.15% at 12h, while in vitro lipolysis test showed fast digestion of the NLCs within 1h. Relative oral bioavailability of SRL-NLCs in Beagle dogs was 1.81-folds that of the commercial nanocrystalline sirolimus tablets Rapamune. In conclusion, the NLCs show potential to improve the oral bioavailability of SRL.

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