Hou J.,Shanghai University College of Sciences |
Zhang M.-X.,Shanghai University |
Dai Z.-H.,Shanghai University |
Li J.-Z.,Shanghai University College of Sciences |
Zeng F.-F.,Shanghai University
Geotextiles and Geomembranes | Year: 2017
The formula for calculating the ultimate bearing capacity of horizontal-vertical reinforced soil is investigated based on the failure mode and the mechanism of sand beds reinforced with horizontal-vertical reinforcement. Two components of soils and reinforcement are calculated separately. The ultimate bearing capacity of a shallow, concentrically loaded strip footing on homogeneous soil is commonly determined using the Terzaghi superposition method. The contribution of horizontal-vertical reinforcement is calculated based on the bearing resistance of the soil against the transverse members. A vertical inclusion is treated as a retaining wall, the confinement being calculated using Rankine's earth pressure theory. An analytical solution is presented including the traditional factors of soil, unit soil weight, footing width, number of horizontal-vertical reinforcement layers, and reinforcement geometry. The results were validated against experimental results and the mean error of the theoretical model was about 10%, with a maximum error of about 20%. © 2016 Elsevier Ltd
Wu Z.,University of Shanghai for Science and Technology |
Li X.,Shanghai University College of Sciences
Proceedings - 2016 9th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics, CISP-BMEI 2016 | Year: 2016
In order to recognize the hand gesture and provide the application in human interaction and rehabilitation training, we designed a system based on wireless surface electromyography signal collection and recognition. The system was mainly composed of the hardware and software. For the hardware design, the center control unit of each module was enhanced by 80C51 MCU. The electrode patch collected surface EMG signals that were amplified by the instrument amplifier AD8422 and A/D conversion, then the information was send to the host computer. For the software design, we extracted the feature of average, root mean square and AR coefficients, and achieved the recognition results by support vector machine. The device is small and portable, i.e. 37mm×27mm×15mm. The part of host computer can meet many kinds of signal analysis as well as the human interaction. © 2016 IEEE.
Lu X.-L.,Shanghai University College of Sciences |
Liu J.-T.,Shanghai University College of Sciences |
Liu J.-T.,China Pharmaceutical University |
Liu X.-Y.,Shanghai University College of Sciences |
And 4 more authors.
Journal of Antibiotics | Year: 2014
Two new diterpenes, libertellenone G(1) and libertellenone H(2) were isolated from the fungus Eutypella sp. D-1 isolated from the soil of high latitude of Arctic, together with two known pimarane diterpenes (3-4). The structures of 1 and 2 were elucidated from spectroscopic data (nuclear magnetic resonance, mass spectrometry and infrared). These compounds were evaluated for cytotoxic activity against seven human tumor cell lines. Compound 2 showed a range of cytotoxicity between 3.31 and 44.1 μM. Compound 1 exhibited antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. © 2014 Japan Antibiotics Research Association.
Xue G.,Shanghai University College of Sciences |
Yan H.-L.,Shanghai University College of Sciences |
Zhang Y.,Shanghai University College of Sciences |
Hao L.-Q.,Shanghai University |
And 3 more authors.
Oncogene | Year: 2014
Previous studies have established the link between aberrant microRNA (miRNA) expression and hypoxia in various neoplasms. However, how these hypoxia-related miRNAs modulate tumor progression is still unclear. Therefore, the patterns of miRNA in colorectal carcinoma cell lines in response to hypoxia or not were first screened and the hypoxia-induced repression of the miR-15-16 cluster was confirmed. Then, this repression was found to be associated with high tumor stage and poor prognosis in colorectal carcinoma and is shown to promote tumor angiogenesis and metastasis by the loss of restriction of its target gene, fibroblast growth factor-2 (FGF2). Moreover, the general and alterative promoters of the miR-15-16 host (deleted in lymphocytic leukemia 2, DLEU2) were mapped, and three c-Myc/Max binding sites in response to the hypoxia-induced repression of miR-15-16 were further identified. Finally, an enhanced stability of c-Myc/Max heterodimer promoted by increased hypoxia-inducible factor-2α (HIF-2α) was validated, and we also verified that the enhancement contributed to the hypoxia-induced repression of miR-15-16. In brief, the c-Myc-mediated transcriptional repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promoted tumor angiogenesis and hematogenous metastasis by the further loss of post-transcriptional inhibition of FGF2. Our study provides a better understanding of the coping mechanisms in response to tumor hypoxia and may be helpful in developing an effective prognostic marker or treatment target against solid tumors.Oncogene advance online publication, 7 April 2014; doi:10.1038/onc.2014.82.
Xue-Song L.,Shanghai University |
Cheng-Zhong L.,Shanghai University |
Ying Z.,Shanghai University College of Sciences |
Mo-Bin W.,Shanghai University
BMC Gastroenterology | Year: 2012
Background: Many studies suggest that in chronic hepatitis B virus (HBV) infection regulate T (Treg) cells and interlukin-17-producing T help cells (Th17) are mutually antagonistic in the immune response. This study is aimed to reveal the cell differentiation environment and the significance of Treg and Th17 balance in the development of acute and chronic HBV infection.Methods: Ten patients with acute HBV infection (AHB) and forty-eight patients with chronic HBV infection, including 12 asymptomatic HBV carriers (HBV carriers), 18 chronic hepatitis B patients (CHB) and 18 acute-on-chronic HBV-related liver failure (ACHBLF) were enrolled. Treg and Th17 cells differentiation related cytokine levels were detected by using ELISA. Flow cytometry was employed to count the Treg and Th17 frequency in peripheral blood.Results: Compared to health controls both AHB and ACHBLF patients favoured Th17 cell differentiation, accompanied by a higher proportion of peripheral Th17 cells (P < 0.01) and high level of interleukin-17A (IL-17A) (P < 0.01). However, asymptomatic HBV carriers and CHB were conducive to Treg cell differentiation. In AHB and ACHBLF, peripheral blood IL-17A + CD4 + T cell frequency increased significantly compared with healthy controls. Changes of Treg and Th17 cell frequency were not completely consistent. Both CHB and ACHBLF had lower level of Treg/Th17 ratio than in health control (P < 0.05). Both plasm IL-17A levels (r = -0.72, p<0.001) and Th17 frequency(r = -0.49, p = 0.0003) negatively correlated with plasma HBV DNA load in patients with chronic HBV infection. In addition, both Th17 frequency and plasm IL-17A levels positively correlated with ALT (r = 0.33,p = 0.01 Vs r = 0.29,p = 0.04) and total bilirubin levels (r = 0.72,p<0.0001 Vs r = 0.53,p = 0.0001) in these chronic HBV-infected subjects. However, for AHB there were positive correlation between both Th17 frequency (r = 0.64, p = 0.04) and plasm IL-17A levels (r = 0.69, p = 0.02) with serum ALT levels, but no significant correlation between both HBV DNA level and total bilirubin level with Th17 frequency or plasm IL-17A levels were found. Furthermore, Treg/Th17 ratio was negatively correlated with total bilirubin levels (r = -0.41, p = 0.004) in chronic HBV-infected patients, especially in patients with ACHBLF (r = -0.69,p = 0.001) and positively correlated with viral load in these chronic HBV-infected subjects (r = 0.55, p<0.0001).Conclusions: Th17 cells are involved in acute and chronic HBV infection, especially in AHB and ACHBLF. CHB and ACHBLF patients manifested obvious Treg/Th17 ratio imbalance, which might be linked to disease progression and the continuous HBV infection. © 2012 Liang X-S et al.; licensee BioMed Central Ltd.
Zhang M.,Shanghai University |
Zhang C.,Shanghai University |
Sun Q.,Shanghai University College of Sciences |
Cai Q.,Shanghai University |
And 2 more authors.
BMC Medical Informatics and Decision Making | Year: 2014
Background: As a part of nationwide healthcare reforms, the Chinese government launched web-based appointment systems (WAS) to provide a solution to problems around outpatient appointments and services. These have been in place in all Chinese public tertiary hospitals since 2009. Methods. Questionnaires were collected from both patients and doctors in one large tertiary public hospital in Shanghai, China.Data were analyzed to measure their satisfaction and views about the WAS. Results: The 1000 outpatients randomly selected for the survey were least satisfied about the waiting time to see a doctor. Even though the WAS provided a much more convenient booking method, only 17% of patients used it. Of the 197 doctors surveyed, over 90% thought it was necessary to provide alternative forms of appointment booking systems for outpatients. However, about 80% of those doctors who were not associated professors would like to provide an 'on-the-spot' appointment option, which would lead to longer waits for patients. Conclusions: Patients were least satisfied about the waiting times. To effectively reduce appointment-waiting times is therefore an urgent issue. Despite the benefits of using the WAS, most patients still registered via the usual method of queuing, suggesting that hospitals and health service providers should promote and encourage the use of the WAS. Furthermore, Chinese health providers need to help doctors to take others' opinions or feedback into consideration when treating patients to minimize the gap between patients' and doctors' opinions. These findings may provide useful information for both practitioners and regulators, and improve recognition of this efficient and useful booking system, which may have far-reaching and positive implications for China's ongoing reforms. © 2014 Zhang et al.; licensee BioMed Central Ltd.
Hu F.,Shanghai University College of Sciences |
Qian Z.-W.,Shanghai University College of Sciences
European Review for Medical and Pharmacological Sciences | Year: 2016
OBJECTIVE: The aim of this study is to analyze the characteristics of white matter lesions in abnormal manifestations of magnetic resonance imaging (MRI) of patients with migraine, and its relationship with migraine. PATIENTS AND METHODS: 38 patients, diagnosed with migraine were continuously selected as the observation group, while during the same period, 30 healthy individuals were selected as the control group. The differences of the features of MRI of two groups, the ratios of white matter lesions manifestations and the severity were compared. RESULTS: The manifestations of MRI in patients with migraine were mainly white matter lesions. The accuracy rate was 86.7%, and the sensibility was 92.3%. According to Wahlund white matter lesions classifications, patients with migraine were mainly Level II and Level III (84.2%). It can be acquired from relevant analyses that white matter lesions classifications and the degrees of migraine were positively correlated (p < 0.05). White matter lesions were mainly distributed in the frontal lobe (78.9%), limbic system (60.5%) and parietal lobe (28.9%). CONCLUSIONS: The MRI of migraine, the manifestations of white matter lesions were relatively sensitive. The higher the classification was linked with the degree of migraine. They were mainly distributed in the frontal lobe, limbic system and parietal lobe.
Wang F.,CAS South China Sea Institute of Oceanology |
Tian X.,CAS South China Sea Institute of Oceanology |
Huang C.,Shanghai University College of Sciences |
Li Q.,CAS South China Sea Institute of Oceanology |
Zhang S.,CAS South China Sea Institute of Oceanology
Journal of Antibiotics | Year: 2011
Three new γ-pyrones named marinactinones A-C (1-3) were isolated from marine-derived actinomycete Marinactinospora thermotolerans SCSIO 00606. These structures were elucidated by extensive spectroscopic methods. All three new compounds were evaluated for cytotoxic effects on six cancer cell lines and inhibitory activities of DNA topoisomerase II. Compounds 1-3 exhibited moderate cytotoxicities against SW1990, HepG2 and SMCC-7721 cell lines, and compound 2 showed weak DNA topoisomerase II inhibition activity. This is the first report on the chemical constituents and their biological activities from Marinactinospora, a novel genus of marine actinomycetes. © 2011 Japan Antibiotics Research Association All rights reserved.
Jiang P.,Shanghai JiaoTong University |
Li C.,Shanghai JiaoTong University |
Xiang Z.,Shanghai University College of Sciences |
Jiao B.,Shanghai University College of Sciences
Molecular Medicine Reports | Year: 2014
Tanshinone IIA (Tan IIA), one of the major active constituents of the medicinal herb Salvia miltiorrhiza, has been reported to possess neuroprotective effects against the pathological features of Alzheimer's disease (AD), but the molecular mechanism underlying this effect remains unclear. To examine the effect of Tan IIA on AD, as well as the underlying molecular mechanisms, in vivo animal experiments and in vitro molecular biology investigations were employed in the present study. Firstly, a rat model of AD was successfully established by direct injection of the amyloid beta protein (Aβ) and then these rats were administered an interventional treatment of Tan IIA. The learning and memory ability of rats was evaluated in four groups (Control, Sham, AD and Tan IIA) utilizing a Morris water maze test. Quantitative (q)PCR was employed to detect the mRNA expression of inducible nitric oxide synthase (iNOS), matrix metalloproteinase-2 (MMP-2) and nuclear transcription factor kappa (NF-κBp65) in temporal lobe tissues and protein expression was determined with western blot analysis. In addition, association analyses between iNOS, MMP-2 and NF-κBp65 at a transcriptional and translational level were performed utilizing Spearman's correlation analysis. In the present study, the results revealed that rats in the AD group demonstrated significant disruptions in learning and memory ability, and the symptoms were evidently reduced by Tan IIA. Furthermore, the upregulation of iNOS, MMP-2 and NF-κBp65 at a transcriptional and translational level in AD rats was distinctly inhibited by Tan IIA. Therefore, it was concluded that iNOS, MMP-2 and NF-κBp65 are involved in AD development, and Tan IIA may reduce AD risk by inhibiting transcription and translation of these genes. Furthermore, the positive correlation of iNOS and MMP-2 with NF-κBp65, respectively, provides evidence supporting the hypothesis that Tan IIA reduces AD risk by inhibiting iNOS and MMP-2 at a transcriptional and translational level through the NF-κB pathway. In summary, Tan IIA is an effective neuroprotective agent for AD therapy, and iNOS, MMP-2 and NF-κBp65 may be the potential molecular targets for manipulating this effect therapeutically.
Guo Y.-J.,Shanghai University College of Sciences |
Wang K.-Y.,Shanghai University College of Sciences |
Sun S.-H.,Shanghai University College of Sciences
Microbes and Infection | Year: 2010
Leptospirosis is an important zoonosis in humans. Immunity against leptospiral infection was thought to be primarily humoral, and limited studies have addressed the role of CD8+ T cells. Leptospiral immunoglobulin-like protein A (LigA) is an important protective antigen of Leptospira and a potential target for Leptospira-specific cell-mediated immunity. In this study, twenty LigA-derived peptides were tested their binding affinity and stability for the HLA-A*0201 molecule. Peptides with high binding affinity and stability for HLA-A*0201 were then assessed their capacity to elicit specific cytotoxic T-lymphocyte (CTL) responses using cytotoxicity, ELISPOT assays for IFN-γ and HLA-A*0201-peptide tetramer assays. We identified a HLA-A*0201-restricted epitope, LigA305-313 KLIVTPAAL in Leptospira LigA. CTLs specific for LigA305-313 were elicited both in HLA-A2.1/Kb transgenic mice and in patients with a clinical and/or laboratory diagnosis of leptospirosis. Staining of the HLA-A*0201-LigA305-313 tetramer revealed the presence of LigA305-313-specific CTLs in peripheral blood mononuclear cells (PBMCs) sourced from five patients infected with three different serovars of Leptospira. In conclusion, we report the existence of specific cytotoxic CD8+ T cells in patients with leptospirosis and we suggest that the newly identified epitope, LigA305-313, will be helpful in enhancing the understanding of the mechanism of immunity to leptospirosis. © 2010 Elsevier Masson SAS. All rights reserved.