Shanghai Tissuebank Biotechnology Co.

Shanghai, China

Shanghai Tissuebank Biotechnology Co.

Shanghai, China
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Zheng Z.,Xiamen University | Zhang P.,Shanghai Tissuebank Biotechnology Co. | He G.,Shanghai Tissuebank Biotechnology Co. | Liao K.,Shanghai Tissuebank Biotechnology Co. | And 5 more authors.
International Journal of Laboratory Hematology | Year: 2017

Introduction: Detection of recurrent genetic abnormalities is of great significance for a refined diagnosis and assessment of prognosis in leukemia. Conventional nested reverse transcription PCR is labor intensive and time-consuming. Methods: We have developed a novel dual-color TaqMan probe-based real-time PCR method for the simultaneous screening of 45 fusion transcripts in 12 parallel reactions. The method was tested and validated with cell lines carrying known fusion transcripts and patient samples. Results: A multiplex real-time PCR method was successfully developed for rapid detection of 45 fusion genes and validated for 15 of the more commonly detected fusion genes. Intra-assay reproducibility assessed for the most frequent rearrangements ranged from 0.41% to 0.74% for the coefficient of variation (CV) of cycle threshold (Ct) and the interassay reproducibility ranged from 1.62% to 2.83% in five separate experiments. The lowest detection limit for the translocations tested ranged between 1 : 16 000 and 1 : 32 000. Validation of the method with 213 patient samples showed 100% specificity and excellent consistence with conventional nested RT-PCR. Conclusion: Overall, we believe that this method is easily applicable, cost-effective, and clinically useful for a rapid screening of fusion genes in the initial diagnostic phase of leukemia. Its use can also be extended to the monitoring of minimal residual disease. © 2017 John Wiley & Sons Ltd.


Pan J.,Shanghai Tissuebank Biotechnology Co. | Chen L.N.,Shanghai Tissuebank Biotechnology Co. | Zhuo X.F.,Shanghai Tissuebank Biotechnology Co. | Huang L.P.,Shanghai Tissuebank Biotechnology Co. | Zheng Z.Z.,Shanghai Tissuebank Biotechnology Co.
Tissue Antigens | Year: 2015

Six novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA in Chinese individuals. © 2015 John Wiley & Sons A/S.


Jiang E.,Tianjin Hematonosis Hospital | Pan J.,Shanghai Tissuebank Biotechnology Co. | Han M.,Tianjin Hematonosis Hospital | Chen L.,Tianjin Hematonosis Hospital | And 7 more authors.
International Journal of Legal Medicine | Year: 2016

Alleles at the D7S820 STR locus have 6–14 different numbers of a four-nucleotide (GATA) repeat motif arranged in tandem. The D7S820 tri-allelic pattern is rare and has not been reported in the Chinese population. In this study we report a three-banded pattern at the D7S820 locus observed in a Chinese family, in which four family members in two generations had tri-allelic D7S820 genotype 10-11-12 and one family member had an abnormal bi-allele genotype 10–11. All of the four tri-allelic cases had the genotype 10-11-12, probably due to three copies of the D7S820 STR sequence in all cells (Type 2 tri-allelic pattern), and deduced alleles 10–11 were a linked inheritance in this family. © 2015, Springer-Verlag Berlin Heidelberg.


PubMed | Shanghai Tissuebank Biotechnology Co. and Xiamen University
Type: | Journal: International journal of laboratory hematology | Year: 2017

Detection of recurrent genetic abnormalities is of great significance for a refined diagnosis and assessment of prognosis in leukemia. Conventional nested reverse transcription PCR is labor intensive and time-consuming.We have developed a novel dual-color TaqMan probe-based real-time PCR method for the simultaneous screening of 45 fusion transcripts in 12 parallel reactions. The method was tested and validated with cell lines carrying known fusion transcripts and patient samples.A multiplex real-time PCR method was successfully developed for rapid detection of 45 fusion genes and validated for 15 of the more commonly detected fusion genes. Intra-assay reproducibility assessed for the most frequent rearrangements ranged from 0.41% to 0.74% for the coefficient of variation (CV) of cycle threshold (Ct) and the interassay reproducibility ranged from 1.62% to 2.83% in five separate experiments. The lowest detection limit for the translocations tested ranged between 1 : 16 000 and 1 : 32 000. Validation of the method with 213 patient samples showed 100% specificity and excellent consistence with conventional nested RT-PCR.Overall, we believe that this method is easily applicable, cost-effective, and clinically useful for a rapid screening of fusion genes in the initial diagnostic phase of leukemia. Its use can also be extended to the monitoring of minimal residual disease.


PubMed | Tianjin Hematonosis Hospital and Shanghai Tissuebank Biotechnology Co.
Type: Journal Article | Journal: International journal of legal medicine | Year: 2016

Alleles at the D7S820 STR locus have 6-14 different numbers of a four-nucleotide (GATA) repeat motif arranged in tandem. The D7S820 tri-allelic pattern is rare and has not been reported in the Chinese population. In this study we report a three-banded pattern at the D7S820 locus observed in a Chinese family, in which four family members in two generations had tri-allelic D7S820 genotype 10-11-12 and one family member had an abnormal bi-allele genotype 10-11. All of the four tri-allelic cases had the genotype 10-11-12, probably due to three copies of the D7S820 STR sequence in all cells (Type 2 tri-allelic pattern), and deduced alleles 10-11 were a linked inheritance in this family.

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