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Moussa M.,Beijing Jiaotong University | Moussa M.,Shanghai JiaoTong University | Moussa M.,The Key Laboratory of Embryo Molecular Biology | Moussa M.,and Shanghai Laboratory of Embryo and Reproduction Engineering | And 11 more authors.
Animal Reproduction Science | Year: 2015

The oocyte is a central regulator of multiple aspects of female fertility, including ovarian follicular development and early embryogenesis. During its prolonged diplotene arrest, the oocyte is subjected to endogenous (i.e., reactive oxygen species from metabolism) and exogenous (i.e., heat stress, malnutrition) sources of damage-inducing factors, which may lead to a progressive deterioration of oocyte quality. A deficit in oocyte competence can lead not only to a failure of fertilization but also to a lower developmental rate after fertilization. Thus, an appropriate environment for growth and maturation of the oocyte, in vivo and in vitro, is critical to ensure optimal oocyte quality. The objectives of the current review are to give an overview of some maternal key factors that influence oocyte quality in cattle and describe some of the findings to date in the hope of obtaining competent oocytes that could be used for clinical and applied purposes. © 2015 Elsevier B.V. Source

Moussa M.,Shanghai JiaoTong University | Moussa M.,The Key Laboratory of Embryo Molecular Biology | Moussa M.,Shanghai Laboratory of Embryo and Reproduction Engineering | Moussa M.,Shanghai Tao Tao Transgenic Corporation | And 10 more authors.
Science China Life Sciences | Year: 2014

Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. © 2014 The Author(s). Source

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