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Zhang W.-B.,Nanjing Medical University | Zhong W.-J.,Shanghai Stomatological Disease Center | Wang L.,Nanjing Medical University
Bone | Year: 2014

Human adipose-derived stem cells (hASCs) have become a highly attractive source of seed cells in bone regenerative. It has become a key issue how to effectively improve osteogenic differentiation of hASCs in the bone tissue engineering.Numerous regulatory pathways dominate osteogenic differentiation of hASCs involve transcriptional factors and signaling molecules. However, how these factors combine with each other to regulate hASCs osteogenic differentiation still remain to be illustrated. The identification of microRNAs will illuminate this and might permit finely tuning the osteogenic differentiation process. Here, we present evidence that miR-218 acts as a positive regulator of hASCs osteogenesis. Real-time PCR shows that miR-218 was up-regulated during osteogenic differentiation. Overexpression of exogenous miR-218 enhanced osteogenic differentiation in vitro, whereas inhibition of miR-218 would suppress osteogenic differentiation. Furthermore, miR-218 directly targeted SFRP2 and DKK2, which is a WNT signaling pathway antagonist, and enhanced Wnt/β-catenin signaling activity. Finally, we found that mimicking Wnt/β-catenin signal strengthened the expression level of miR-218, while blocking the signal attenuated the expression level of miR-218. This feed-forward regulatory circuit provides additional insight into how miRNAs acting as a signal amplifier interact with signal molecules during hASCs osteogenic differentiation. Taken together, we have established a regulatory network with a central role for the miR-218 in hASCs osteogenic differentiation. © 2013 Elsevier Inc. Source


Qiao G.,Tongji University | Qiao G.,Shanghai Stomatological Disease Center | Su J.,Tongji University | He M.,Shanghai JiaoTong University
Dental Materials Journal | Year: 2012

The aim of this study was to investigate the electrochemical behavior of Co-Cr alloy in artificial saliva containing (-)-epigallocatechin gallate (EGCG) and to characterize the composition and structure of the passive film formed by potentiodynamic polarization. Electrochemical measurements ranked the corrosion resistance of Co-Cr dental cast alloy as follows when exposed to artificial saliva containing different concentrations of EGCG: 4.0 g/L<2.0 g/L<0 g/L<0.5 g/L<1.0 g/L. This showed that the concentration of EGCG in artificial saliva affected the corrosion behavior of Co-Cr alloy. X-ray photoelectron spectroscopy (XPS) results revealed that the outermost surface layer on Co-Cr alloy was mainly composed of Cr2O3 and EGCG-Cr (III) ion complex, which were formed by redox and complex formation reactions respectively. When the redox reaction was dominant, Co-Cr alloy exhibited high corrosion resistance in EGCG-containing artificial saliva. On the contrary, when the complex formation reaction was dominant, Co-Cr alloy exhibited low corrosion resistance. Source


Pan J.,Shanghai Stomatological Disease Center | Zhao J.,Shanghai JiaoTong University | Jiang N.,Shanghai JiaoTong University
Journal of Applied Oral Science | Year: 2014

Objective: The immune compromised patients after treatment of oral cancer may have a chance of infection by drug-resistant opportunistic microbes. We investigated the occurrence of opportunistic microorganisms in aged individuals receiving follow-up examinations after treatment of oral cancer in China. Material and Methods: These patients were used as test group and the respective age grouped healthy individuals as control group. In this study, the oral cavity microorganisms such as bacteria and yeast were taken for the analysis. After the screening of representative microorganisms, their aptitude of pervasiveness against drugs was studied. Here, we used antimicrobial agents which are common in clinical practice. We also performed studies to investigate the presence of toxin genes in methicillin-resistant S. aureus (MRSA). Results: The results indicate that the prevalence of drug-resistant microbes was more pronounced in oral cancer patients after initial treatment above 70 years old. The oxacillin resistance of S. aureus isolate compromise in elderly patients. Conclusions: This study reveals the occurrence of drugresistant opportunistic microorganisms in oral cavity after treatment for oral cancer in aged individuals. Special attention should be directed to MRSA during the treatment of oral cancer, and to realize the fact of immune compromise in elderly patients. Source


Yu H.,Shanghai JiaoTong University | Jiao F.,Shanghai Stomatological Disease Center | Wang B.,Shanghai JiaoTong University | Shen S.G.,Shanghai JiaoTong University
Journal of Craniofacial Surgery | Year: 2013

Our aim was to evaluate the application of piezoelectric decortication in periodontally accelerated osteogenic orthodontics (PAOO). One hundred fifty-six patients with severe skeletal malocclusions were enrolled in this study. Ultrasonic decortications were performed in 187 labial or lingual PAOO of the maxillary and mandibular anterior teeth. Orthodontic decompensation started from the fifth day after operation. All patients healed uneventfully and no severe periodontic complications were recorded. Rapid teeth movement and relatively short treatment duration were realized. Alveolar fenestration and bony dehiscence was successfully addressed. With physical and mechanical properties of absence of macrovibration, ease of use and control, piezosurgery showed its great values in PAOO. Copyright © 2013 by Mutaz B. Habal, MD. Source


Li G.Q.,Shanghai Stomatological Disease Center
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology | Year: 2012

To investigate the marginal adaptation of crowns fabricated by selective laser melting (SLM) and wax-lost-casting method, so as to provide an experimental basis for clinic. Co-Cr alloy full crown were fabricated by SLM and wax-lost-casting for 24 samples in each group. All crowns were cemented with zinc phosphate cement and cut along longitudinal axis by line cutting machine. The gap between crown tissue surface and die was measured by 6-point measuring method with scanning electron microscope (SEM). The marginal adaptation of crowns fabricated by SLM and wax-lost-casting were compared statistically. The gap between SLM crowns were (36.51 ± 2.94), (49.36 ± 3.31), (56.48 ± 3.35), (42.20 ± 3.60) μm, and wax-lost-casting crowns were (68.86 ± 5.41), (58.86 ± 6.10), (70.62 ± 5.79), (69.90 ± 6.00) μm. There were significant difference between two groups (P < 0.05). Co-Cr alloy full crown fabricated by wax-lost-casting method and SLM method provide acceptable marginal adaptation in clinic, and the marginal adaptation of SLM is better than that of wax-lost-casting method. Source

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