Shanghai Skin Disease Hospital
Shanghai Skin Disease Hospital
Chen Z.,Fudan University |
Chen Z.,Shanghai Skin Disease Hospital |
Lu Y.,Fudan University |
Qi J.,Fudan University |
Wu W.,Fudan University
Pharmaceutical Development and Technology | Year: 2013
The aim of this work was to prepare stable all-trans-retinoic acid (ATRA)/2-hydroxypropyl-β-cyclodextrin (HPCD) inclusion complex pellets with industrial feasible technology, the fluid-bed coating technique, using PVP K30 simultaneously as binder and reprecipitation retarder. The coating process was fluent with high coating efficiency. In vitro dissolution of the inclusion complex pellets in 5% w/v Cremopher EL solution was dramatically enhanced with no reprecipitation observed, and significantly improved stability against humidity (92.5% and 75% RH) and illumination (4500 lx ± 500 lx) was achieved by HPCD inclusion. Differential scanning calorimetry and powder X-ray diffractometry confirmed the absence of crystallinity of ATRA. Fourier transform-infrared spectrometry revealed interaction between ATRA and HPCD adding evidence on inclusion of ATRA moieties into HPCD cavities. Solid-state 13C NMR spectrometry indicated possible inclusion of ATRA through the polyene chain, which was the main reason for the enhanced photostability. It is concluded that the fluid-bed coating technique has the potential use in the industrial preparation of ATRA/HPCD inclusion complex pellets. © 2013 Informa Healthcare USA, Inc.
Shen F.,Shanghai Skin Disease Hospital |
Wang Q.-L.,Shanghai Skin Disease Hospital
Journal of Clinical Dermatology | Year: 2017
A case of bullous acute febrile neutrophilic dermatosis is reported. A 51-year-old male presented with red plaques and bullae on the face and upper limbs accompanied with fever and high level of leucocytes for about 10 days. Histopathological examination revealed focal epidermal edema, severe edema in the dermal papillae, separation between the epidermis and dermis, dense lymphocytes and neutrophilic granulocytes in the upper dermis, extravasation of red blood cells with nuclear deposition, and perivascular inflammation in the middle dermis. Direct immunofluorescence showed negative results for IgG, IgM, IgA and C3. The diagnosis of bullous acute febrile neutrophilic dermatosis was made.
Zou Y.,Shanghai Skin Disease Hospital |
Ge M.,Changhai Hospital |
Wang X.,Shanghai Skin Disease Hospital
Biochemical and Biophysical Research Communications | Year: 2017
Abnormal activation of PI3K-AKT-mTOR signaling is detected in human skin squamous cell carcinoma (SCC). LY3023414 is a novel, potent, and orally bio-available PI3K-AKT-mTOR inhibitor. Its activity against human skin SCC cells was tested. We demonstrated that LY3023414 was cytotoxic when added to established (A431 line) and primary (patient-derived) human skin SCC cells. LY3023414 induced G0/1-S arrest and inhibited proliferation of skin SCC cells. Moreover, LY3023414 induced activation of caspase-3/-9 and apoptosis in skin SCC cells. Intriguingly, LY3023414 was yet non-cytotoxic nor pro-apoptotic to normal human skin cells (melanocytes, keratinocytes and fibroblasts). At the molecular level, LY3023414 blocked PI3K-AKT-mTOR activation in skin SCC cells, as it dephosphorylated PI3K-AKT-mTOR substrates: P85, AKT and S6K1. In vivo studies showed that oral administration of LY3023414 at well-tolerated doses inhibited A431 xenograft tumor growth in severe combined immunodeficiency (SCID) mice. AKT-mTOR activation in LY3023414-treated tumors was also largely inhibited. Together, these results suggest that targeting PI3K-AKT-mTOR by LY3023414 inhibits human skin SCC cell growth in vitro and in vivo, establishing the rationale for further clinical testing. © 2017 Elsevier Inc.
Li K.,Shanghai Skin Disease Hospital |
Li K.,University of California at San Francisco |
Wang C.,Shanghai Skin Disease Hospital |
Lu H.,Shanghai Skin Disease Hospital |
And 3 more authors.
PLoS Neglected Tropical Diseases | Year: 2013
Background:Syphilis, a sexually transmitted disease caused by spirochetal bacterium Treponema pallidum, can progress to affect the central nervous system, causing neurosyphilis. Accumulating evidence suggest that regulatory T cells (Tregs) may play an important role in the pathogenesis of syphilis. However, little is known about Treg response in neurosyphilis.Methodology/Principal Findings:We analyzed Treg frequencies and Transforming Growth Factor-β (TGF-β) levels in the blood and CSF of 431 syphilis patients without neurological involvement, 100 neurosyphilis patients and 100 healthy donors. Suppressive function of Tregs in peripheral blood was also assessed. Among syphilis patients without neurological involvement, we found that secondary and serofast patients had increased Treg percentages, suppressive function and TGF-β levels in peripheral blood compared to healthy donors. Serum Rapid Plasma Reagin (RPR) titers were positively correlated with Treg numbers in these patients. Compared to these syphilis patients without neurological involvement, neurosyphilis patients had higher Treg frequency in peripheral blood. In the central nervous system, neurosyphilis patients had higher numbers of leukocytes in CSF compared to syphilis patients without neurological involvement. CD4+ T cells were the predominant cell type in the inflammatory infiltrates in CSF of neurosyphilis patients. Interestingly, among these neurosyphilis patients, a significant decrease in CSF CD4+ CD25high Treg percentage and number was observed in symptomatic neurosyphilis patients compared to those of asymptomatic neurosyphilis patients, which may be associated with low CSF TGF-β levels.Conclusions:Our findings suggest that Tregs might play an important role in both bacterial persistence and neurologic compromise in the pathogenesis of syphilis. © 2013 Li et al.
Gu W.,Shanghai Skin Disease Hospital |
Yang Y.,Shanghai Skin Disease Hospital |
Wu L.,Shanghai Skin Disease Hospital |
Yang S.,Shanghai Skin Disease Hospital |
Ng L.-K.,Public Health Agency of Canada
BMJ Open | Year: 2013
Objective: In this study, we aimed to determine the performance characteristics of toluidine red unheated serum test on cerebrospinal fluids (CSF-TRUST) as compared to venereal disease research laboratory test on cerebrospinal fluids (CSF-VDRL) for laboratory the diagnosis of neurosyphilis. Design: A cross-sectional study. Setting: Sexually transmitted infections (STIs) clinics. Participants and methods: CSF and serum samples were collected from 824 individual STD clinic patients who have syphilis and are suspected to progress to neurosyphilis within a 9-month period. CSF-VDRL and CSF-TRUST were performed parallelly on the same day when collected. Treponema pallidum particle agglutination (TPPA) tests were also performed on the CSF and the serum samples, and biochemical analysis of the CSF samples was also performed. Results: The overall agreement between CSF-TRUST and CSF-VDRL was 97.3%. The reactive ratios of the CSF samples were 22.1% by CSF-TRUST and 24.8% by CSF-VDRL, respectively. All CSF-TRUST-reactive cases were reactive in the CSF-VDRL. Twenty-two samples with CSF-TRUST-negative were tested CSFVDRL- reactive with low titres (1 : 1 to 1 : 4). Over 97% of the double-reactive CSF samples (CSF-VDRL and CSF-TRUST) had an identical titre or a titre within a two-fold difference. The agreement of CSF-TPPA and CSF-VDRL was 71.9%. Similarly, the agreement of CSF-TPPA and CSF-TRUST was 69.2%. Conclusions: Our results revealed that CSF-TRUST could be used as an option for CSF examination in settings without CSF-VDRL in place.
Zhang S.,Fudan University |
Yu N.,Shanghai Skin Disease Hospital |
Zhang R.,Fudan University |
Wu J.,Fudan University
Investigative Ophthalmology and Visual Science | Year: 2016
PURPOSE. Inflammasome activation and IL-1β production have been proposed to have an important role in age-related macular degeneration (AMD). Growing evidence is emerging for involvement of interleukin-17A (IL-17A) in AMD pathogenesis. We investigated the effects of IL-17A on the activation of inflammasome and production of IL-1β in primary human RPE cells. METHODS. Primary human RPE cells were isolated and cultured for the following experiments. Expression patterns of IL-17 receptor A (IL-17RA), IL-17 receptor C (IL-17RC), and ACT1 were analyzed by RT-PCR, flow cytometry, and immunofluorescence. IL-17A was added to the cell cultures, and cytokine expression, signaling pathways, and inflammasome machinery were investigated using real-time RT-PCR, ELISA, Western blot, flow cytometry, and small interfering RNA. RESULTS. Retinal pigment epithelial cells constitutively expressed IL-17RA, IL-17RC, and ACT1. IL-17A upregulated the mRNA levels of pro-IL-1β, IL-8, CCL2, and CCL20, as well as the protein level of IL-1β. IL-17A induced the phosphorylation of Akt, Erk1/2, p38 MAPK, and NFjB p65 in RPE cells. Blocking NF-jB attenuated IL-17A–induced expression of pro-IL-1β mRNA. IL-17A enhanced pro-caspase-1 and NLRP3 mRNA expression. Inhibiting caspase-1 activity and silencing NLRP3 decreased IL-1b secretion, confirming NLRP3 as the IL-17A– responsive inflammasome on the posttranscriptional level. The mechanism of IL-17A– triggered NLRP3 activation and subsequent IL-1β secretion was found to involve the generation of reactive oxygen species. CONCLUSIONS. Our results suggest that IL-17A triggers a key inflammatory mediator, IL-1β, from RPE cells, via NLRP3 inflammasome activation, holding therapeutic potential for AMD. © 2016. Sci all right reserved.
Liu N.,Shanghai Skin Disease Hospital |
Wang X.,Shanghai Skin Disease Hospital |
Odio M.,Procter and Gamble
Pediatric Dermatology | Year: 2011
Chinese cloth diapers differ from disposable diapers in several respects that are central to our understanding of the etiology of diaper dermatitis (DD), yet there are no published reports on the dermatological correlates of this manner of infant care, which is prevalent among the world's second-largest pediatric population. The objective was to determine the prevalence and severity of DD in exclusive users of Chinese cloth diapers. This observational study was conducted during a single home visit to 694 Chinese children who were exclusive users of cloth diapers in five inland cities of China. During each home visit, study nurses documented the presence and severity of DD using a visual dermatological scale and conducted transepidermal water loss (TEWL) and pH measurements. Diaper dermatitis was rare in the buttocks and genital area of the children (<20%) but was common in the perianal and intertriginous regions (50-70%). TEWL and pH were mildly higher in babies with DD than in those without DD in the genital and buttocks area. Diaper dermatitis is common in children who use traditional Chinese cloth diapers, especially in the perianal and intertriginous regions. © 2011 Wiley Periodicals, Inc.
Fan G.-B.,Shanghai Skin Disease Hospital |
Wu P.-L.,Shanghai Skin Disease Hospital |
Wang X.-M.,Shanghai Skin Disease Hospital
Skin Research and Technology | Year: 2012
Background/purpose: Cigarette smoking not only causes systemic health problems, but may also be an underlying cause of premature skin aging. Cigarette smokers frequently have morphological changes in facial skin that may be attributed to reduced oxygen in this region. The purpose of this study was to measure the oxygen content in facial skin before and after smoking. Methods: Twenty-five volunteers participated in this study. Changes in oxygen content of the facial skin were measured before and after 30 min of cigarette smoking. Skin temperature and oxygen content were evaluated in the periorbital and periolar regions. Results: There was a significant increase in temperature after smoking. The oxy hemoglobin and partial pressure of oxygen decreased in both the periocular and perioral areas after smoking. There were no changes in deoxy hemoglobin and partial pressure of carbon dioxide at these areas. Conclusion: Significant changes were seen in temperature and oxygen content after only 30 min of smoking. The results from this study suggest that alterations in the skin temperature and oxygen content in facial skin after smoking may be an underlying cause of premature skin aging. © 2011 John Wiley & Sons A/S.
Zhou P.,Shanghai Skin Disease Hospital |
Gu X.,Shanghai Skin Disease Hospital |
Lu H.,Shanghai Skin Disease Hospital |
Guan Z.,Shanghai Skin Disease Hospital |
Qian Y.,Shanghai Skin Disease Hospital
Sexually Transmitted Infections | Year: 2012
Objectives: To study 17 cases of secondary syphilis that progressed to neurosyphilis despite appropriate treatments and whose rapid plasma reagin (RPR) titres showed a fourfold decrease within 6 months but did not revert to negative. Methods: Secondary syphilis patients with the following criteria were analysed: (1) RPR titres declined fourfold within 3 months after therapy, (2) patients denied high-risk sexual behaviours following treatment, (3) RPR titre remained serofast 24 months after treatment, (4) reactive cerebrospinal fluid (CSF) - venereal disease research laboratory (VDRL) and CSF - Treponema pallidum Particle Agglutination Test (TPPA) and (5) HIV antibody negative. Results: 14 male and three female patients met the criteria. 13 patients were asymptomatic. The CSF leucocyte count was elevated in 10 patients of whom nine also had elevated CSF - proteins. The RPR titres following secondary syphilis treatments were ≥1:32 in five cases, 1:16 in four cases, 1:8 in six cases and 1:4 in two cases. Following treatments for neurosyphilis, four cases with neurological or psychiatric manifestations resolved or improved, nine cases with raised CSF - white blood cells returned to normal and nine of 12 cases with raised CSF - protein declined to normal. Conclusions: Neurosyphilis may be detected in immunocompetent patients despite appropriate therapy for early-stage syphilis and appropriate serological responses. Clinicians should consider a CSF examination in any treated patient with evidence of disease progression irrespective of prior treatment history and serological response.
Ding Y.,Shanghai Skin Disease Hospital |
Yi X.,Shanghai Skin Disease Hospital |
Yu N.,Shanghai Skin Disease Hospital
Clinical and Experimental Dermatology | Year: 2013
Psoriasis is characterized by a T-helper (Th)1/Th17 immune response, and an increase in IgE levels is a prototypical marker of Th2 immunity. The aim of this retrospective case-control study was to analyse serum total IgE levels in generalized pustular psoriasis (GPP), a rare and severe variant of psoriasis. The levels of IgE in patients with GPP and patients with psoriasis vulgaris (PV) were compared with those of healthy controls. IgE levels were also compared with the levels of C-reactive protein (CRP), an indicator of systemic inflammation. The percentage of patients with GPP who had increased IgE levels was significantly higher than that of patients with PV and of healthy controls. The mean levels of IgE were also higher in the GPP group. The IgE levels in patients with GPP had a significant positive correlation with CRP levels. We hypothesize that serum IgE level is a general indicator for increased inflammation in GPP and PV. Click here for the corresponding questions to this CME article. © 2013 British Association of Dermatologists.