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Fang L.-X.,Shanghai University of Traditional Chinese Medicine | Xiong A.-Z.,Shanghai University of Traditional Chinese Medicine | Wang R.,Shanghai University of Traditional Chinese Medicine | Wang R.,Shanghai Randnter for Standardization of Chinese Medicines | And 5 more authors.
Journal of Separation Science | Year: 2013

The objective of this study was to develop an effective strategy for screening and identifying mycotoxins in herbal medicine (HM). Here, Imperatae Rhizoma, a commonly used Chinese herb, was selected as a model HM. A crude drug contaminated with fungi was analyzed by comparing with uncontaminated ones. Ultra-performance LC coupled to tandem quadrupole TOF-MS (UPLC-Q-TOF-MS) with collision energy function was applied to analyze different samples from Imperatae Rhizoma. Then, MarkerLynxTM software was employed to screen the excess components in analytes, compared with control samples, and those selected markers were likely to be the metabolites of fungi. Furthermore, each of the accurate masses of the markers obtained from MarkerLynxTM was then searched in a mycotoxins/fungal metabolites database established in advance. The molecular formulas with relative mass error between the measured and theoretical mass within 5 ppm were chosen and then applied to MassFragmentTM analysis for further confirmation of their structures. With the use of this approach, five mycotoxins that have never been reported in HM were identified in contaminated Imperatae Rhizoma. The results demonstrate the potential of UPLC-Q-TOF-MS coupled with the MarkerLynxTM software and MassFragmentTM tool as an efficient and convenient method to screen and identify mycotoxins in herbal materials and aid in the quality control of HM. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Zhao T.,Shanghai University of Traditional Chinese Medicine | Zheng S.-S.,Shanghai University of Traditional Chinese Medicine | Zhang B.-F.,Shanghai University of Traditional Chinese Medicine | Zhang B.-F.,Shanghai Randnter for Standardization of Chinese Medicines | And 6 more authors.
Food Chemistry | Year: 2012

The β-carboline alkaloids, harmaline and harmine, are present in hallucinogenic plants Ayahuasca and Peganum harmala, and in a variety of foods. In order to establish the metabolic pathway and bioactivities of endogenous and xenobiotic bioactive β-carbolines, high-performance liquid chromatography, coupled with mass spectrometry, was used to identify these metabolites in human liver microsomes (HLMs) in vitro and in rat urine and bile samples after oral administration of the alkaloids. Three metabolites of harmaline and two of harmine were found in the HLMs. Nine metabolites for harmaline and seven metabolites for harmine, from the rat urine and bile samples, were identified. Among them, four in vivo metabolites were isolated and fully characterised by NMR analysis. For the first time, harmaline is shown transforming to harmine by oxidative dehydrogenation in rat. Five metabolic pathways were therefore proposed, namely, oxidative dehydrogenation, 7-O-demethylation, hydroxylation, O-glucuronide conjugation and O-sulphate conjugation. © 2012 Elsevier Ltd. All rights reserved. Source


Chen J.-Z.,China Pharmaceutical University | Chou G.-X.,Shanghai University of Traditional Chinese Medicine | Chou G.-X.,Shanghai Randnter for Standardization of Chinese Medicines | Wang C.-H.,Shanghai University of Traditional Chinese Medicine | And 5 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2010

Norisoboldine (1,9-dihydroxy-2,10-dimethoxynoraporphine) is one of the major bioactive isoquinoline alkaloids in Linderae Radix, a commonly used Chinese herbal medicine. The aim of this study is to isolate and characterize metabolites of norisoboldine after gavage feeding in rats. High-performance liquid chromatography coupled with electrospray ionization and ion-trap mass spectrometry (HPLC-ESI/MSn) was used to identify metabolites of norisoboldine in rat urine and bile samples. A total of five metabolites of norisoboldine were characterized by comparing retention time and UV absorption in HPLC, and by molecular mass and fragmentation pattern of the analytes by mass spectrometry with those of norisoboldine. Two new glucuronide conjugates of norisoboldine, noriosboldine-1-O-β-d-glucuronide and norisoboldine-9-O-α-d-glucuronide, were isolated from rat urine samples and their structures were confirmed by NMR spectroscopy (1H, 13C, HMBC and HSQC) for the first time. The results suggested that glucuronidation and sulfation were involved in metabolic pathways of norisoboldine in rat. © 2010 Elsevier B.V. Source


Shen K.,Shanghai University of Traditional Chinese Medicine | Ji L.,Shanghai University of Traditional Chinese Medicine | Ji L.,Shanghai Randnter for Standardization of Chinese Medicines | Gong C.,Shanghai University of Traditional Chinese Medicine | And 7 more authors.
Biochemical Pharmacology | Year: 2012

Notoginsenoside Ft1 (Ft1) is a saponin isolated from Panax notoginseng, which has been used traditionally for the treatment of trauma injuries in East Asia. Here we show that Ft1 is a novel stimulator of angiogenesis. The results show that Ft1 induces proliferation, migration, and tube formation in cultured human umbilical vein endothelial cells (HUVECs). Ft1 increases translocalization of hypoxia-inducible factor-1α (HIF-1α) from cytoplasm to nuclei, where it binds to the vascular endothelial growth factor (VEGF) promoter, increasing the expression of VEGF mRNA and the subsequent secretion of the growth factor. Ft1 induces the activation of PI3K/AKT and Raf/MEK/ERK signaling pathways. Pharmacological inhibition with LY294002, wortmanin or PD98059 reduces Ft1-induced angiogenesis, indicating the important role played by these pathways. In addition, Ft1 induces phosphorylation of the mammalian target of rapamycin (mTOR), and siRNA-mediated mTOR knockdown decreases tube formation, proliferation, transport of HIF-1α into nuclei and VEGF mRNA expression in response to Ft1. Finally, in vivo, Ft1 promotes the formation of blood vessels in Matrigel plug and wound healing in mice. Taken together, the present results reveal that Ft1 stimulates angiogenesis via HIF-1α-mediated VEGF expression, with PI3K/AKT and Raf/MEK/ERK signaling cascades concurrently participating in the process. © 2012 Elsevier Inc. All rights reserved. Source


Liu Q.,Shanghai Randnter for Standardization of Chinese Medicines | Chou G.-X.,Shanghai Randnter for Standardization of Chinese Medicines | Chou G.-X.,Shanghai University of Traditional Chinese Medicine | Wang Z.-T.,Shanghai Randnter for Standardization of Chinese Medicines | Wang Z.-T.,Shanghai University of Traditional Chinese Medicine
Helvetica Chimica Acta | Year: 2012

One new iridoid glucoside, 4″-O-β-D-glucosyl-6′-O-(4-O- β-D-glucosylcaffeoyl)linearoside (1), and two new secoiridoid glucosides, 6′-O-acetylsweroside (2) and 6′-O-acetyl-3′-O-[3-(β-D- glucopyranosyloxy)-2-hydroxybenzoyl]sweroside (3), were isolated from the dried roots of Gentiana manshurica (Gentianaceae), together with 11 known ones, including one iridoid glucoside, five secoiridoid glucosides, and five triterpenes. The structures of the new compounds were determined on the basis of detailed spectroscopic analyses and acidic hydrolysis. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland. Source

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