Shanghai R and nter for Standardization of Traditional Chinese Medicines

Shanghai, China

Shanghai R and nter for Standardization of Traditional Chinese Medicines

Shanghai, China
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Geng F.,Shanghai University of Traditional Chinese Medicine | Yang L.,Shanghai University of Traditional Chinese Medicine | Yang L.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Chou G.,Shanghai University of Traditional Chinese Medicine | And 3 more authors.
Phytotherapy Research | Year: 2010

Ethanolic extract of the seeds of Plantago asiatica L. showed significant inhibitory activity of angiotensinconverting enzyme (ACE) determined by monitoring the transformation from a substrate hippuryl-histidylleucine (HHL) to the product hippuric acid (HA) in vitro using an UPLC-MS method. The bioguided fractionation of the extract resulted in the isolation of four ACE inhibitory active phenylpropanoid glycosides acteoside, isoacteoside, plantainoside D, and plantamajoside with IC50 values of 2.69 mM, 2.46 mM, 2.17 mM, and 2.47 mM, respectively. Their structures were elucidated through the analysis of NMR, UV, IR and MS data. Our study is the first demonstration that Plantago asiatica L. and its major constituents have ACE inhibitory activity in vitro. It is assumed that the identified compounds contribute to the angiotensin- converting enzyme-inhibitory activity of the extract. Copyright © 2009 John Wiley & Sons, Ltd.


Zhou Q.,Shanghai University of Traditional Chinese Medicine | Zhou Q.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Zhong L.,Shanghai University of Traditional Chinese Medicine | Zhong L.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | And 8 more authors.
International Journal of Pharmaceutics | Year: 2013

The present study aimed to prepare a chemically and physically stable formulation of baicalein (Ba) in an in situ thermally sensitive hydrogel for vaginal administration. An inclusion complex of Ba and hydroxypropyl-γ- cyclodextrin (HP-γ-CD) was first developed to increase the stability and solubility of Ba in an aqueous solution. The formation of the Ba-HP-γ-CD complex was characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and X-ray powder diffractometry (XRPD). Poloxamers 407, 188 (P407, P188), sodium alginate (SA), hydroxypropylmethylcellulose (HPMC), and benzalkonium bromide were mixed to obtain a hydrogel with an appropriate gelation temperature for vaginal use. Among the formulations examined, P407/Ba-HP-γ-CD/HPMC/P188/benzalkonium bromide (18/0.96/0.5/4.0/0.02%) represented the appropriate gelation temperature and acceptable drug release rate at the administration site. Release of Ba-HP-γ-CD from the poloxamer hydrogel followed Peppas equation, which suggests that it occurred through coupled corrosion-diffusion mechanism, and the corrosion-release curve showed that corrosion was the primary mode of release. In animal study, treatment by using Ba-HP-γ-CD thermosensitive hydrogel could produce a restoration of damaged tissues. Thermosensitive hydrogel formulation of the Ba-HP-γ-CD complex appears to be a promising treatment for cervicitis. © 2013 Elsevier B.V. All rights reserved.


Ding L.-L.,China Pharmaceutical University | Ding L.-L.,Shanghai University of Traditional Chinese Medicine | Ding L.-L.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Zhang B.-F.,Shanghai University of Traditional Chinese Medicine | And 9 more authors.
Journal of Ethnopharmacology | Year: 2012

Ethnopharmacological relevance: Danning tablet, as a composite prescription of traditional Chinese medicine, has been used clinically to relieve liver and gallbladder diseases in China. However, the mechanisms involved are still unclear. Aim of the study: The present investigation was designed to assess the effects and possible mechanisms of Danning tablet on α- naphthylisothiocyanate (ANIT)-induced liver injury with cholestasis. Materials and methods: Danning tablet (3, 1.5 or 0.75 g/kg body weight/day) was intragastrically (i.g.) given to experimental rats for seven days before they were treated with ANIT (60 mg/kg daily via i.g.) which caused liver injury. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyltranspeptidase (γ-GTP), total bilirubin (T-Bil), direct bilirubin (D-Bil), total bile acid (TBA) and bile flow were measured to evaluate the protective effect of Danning tablet at 48 h after ANIT treatment. Furthermore, protective mechanisms of Danning tablet against ANIT-induced liver injury were elucidated by assays of liver enzyme activities and component contents including myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (Gpx), catalase (CAT) and glutathione S-transferase (GST), as well as liver lipid peroxide (LPO) and glutathione (GSH). The biochemical observations were supplemented by histopathological examination. Phytochemical analysis of Danning tablet was performed by UPLC-MASS. Results: Obtained results demonstrated that high dose (3 g/kg) of Danning tablet significantly prevented ANIT-induced changes in bile flow (P < 0.01), and serum levels of ALT, AST, ALP, γ-GTP, T-Bil, D-Bil (P < 0.01) and TBA (P < 0.05). In addition, ANIT-induced increases in hepatic MPO, GST activities and GSH, LPO contents were significantly (P < 0.01) reduced, while SOD, Gpx, CAT activities in the liver tissue which were suppressed by ANIT were significantly (P < 0.01) elevated in the groups pretreated with Danning tablet at the dose of 3 g/kg B.W. Histopathology of the liver tissue showed that pathological injuries were relieved after Danning tablet (3 g/kg) pretreatment. The results also showed that medium dose (1.5 g/kg) of Danning tablet exhibited partially protective effect on ANIT-induced liver injury with cholestasis by reversing part of biochemical parameters and histopathological changes. Low dose (0.75 g/kg) of Danning tablet did not show any protective effect on ANIT-induced liver injury with cholestasis. Phytochemical analyses revealed the presence of anthraquinones, flavonoids and stilbene in the Danning tablet. Conclusion: These findings indicate that Danning tablet exerts a dose-dependently protective effect on ANIT-induced liver injury with cholestasis in rats, and the possible mechanism of this activity is likely due to its attenuation of oxidative stress in the liver tissue and neutrophil infiltration. © 2011 Elsevier Ireland Ltd. All rights reserved.


Huang S.,Shanghai University | Yang Q.,Shanghai University | Shi Y.,Shanghai University | Wang R.,Shanghai University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining paeoniflorin and albiflorin in Paeoniae Radix Alba Method: Using paeoniflorin as the internal reference substance, the relative correction factor (RCF) of albiflorin was determined by HPLC and UPLC with good reproducibility. The contents of paeoniflorin in 16 samples of Paeoniae Radix Alba were authentically determined by the external standard method, and the content of albiflorin was calculated according to the RCF. The contents of these two components in the samples were determined with the external standard method. Result: No significant differences between the quantitative results of QAMS method and external standard method were observe. Conclusion: It is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable. It can be used to control the quality of Paeoniae Radix Alba.


Yang Q.,Shanghai University | Yang L.,Shanghai University | Yang L.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Xiong A.,Shanghai University | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. Method: To estabolish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography- mass spectrometry (UPLC-MS) , to find out the potential identification biomarker by PLS-DA. Result: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione(GSH) , γ-aminobutyric acid (GABA) , prostaglandin F2α(PGF2α) , prostaglandinE3 (PGE3) , leukotrieneA4 (LTA4) ,prostaglandinE2(PGE2)are proven to be siginificant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2α and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. Conclusion: The results of metabolomics are related with the results of classic pharmaco- experiment , which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.


Wu L.-H.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Wu L.-H.,Shanghai University of Traditional Chinese Medicine | Leon C.J.,Jodrell Laboratory | Wang Z.-T.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Wang Z.-T.,Shanghai University of Traditional Chinese Medicine
Annales Botanici Fennici | Year: 2012

Gentiana zhenxiongensis L.H. Wu & Z.T. Wang sp. nova (Gentianaceae) from Yunnan province, China, is described and illustrated. It resembles G. praticola, but differs in having stolons and/or rhizomes, small flowers, calyx lobes with keeled midribs, a campanulate corolla and a tuberculate testa. © Finnish Zoological and Botanical Publishing Board 2012.


Ding L.,Shanghai University of Traditional Chinese Medicine | Ding L.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Zhang B.,Shanghai University of Traditional Chinese Medicine | Zhang B.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | And 5 more authors.
BMC Complementary and Alternative Medicine | Year: 2014

Background: The Danning tablets (DNts) is commonly prescribed in China as a cholagogic formula. Our previous studies showed that DNts exerted the protective effect on α-naphthylisothiocyanate (ANIT)-induced liver injury with cholestasis in a dose-dependent mannar. However, the detailed molecular mechanisms of DNts against ANIT-induced cholestasis are still not fully explored.Methods: Danning tablet (3 g/kg body weight/day) was administered orally to experimental rats for seven days before they were treated with ANIT (60 mg/kg daily via gastrogavage) which caused cholestasis. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (T-Bil), direct bilirubin (D-Bil) and total bile acid (TBA) were measured to evaluate the protective effect of Danning tablet at 12, 24 and 48h after ANIT treatment. Meanwhile, total bilirubin or total bile acid in the bile, urine and liver were also measured at 48h after ANIT treatment. Furthermore, the hepatic or renal mRNA and protein levels of metabolic enzymes and transports were investigated to elucidate the protective mechanisms of Danning tablet against ANIT-induced cholestasis.Results: In this study, we found that DNts significantly attenuated translocation of multidrug resistance-associated protein 2 (Mrp2) from the canalicular membrane into an intracellular and up-regulated the hepatic mRNA and protein expressions of metabolic enzymes including cytochrome P450 2b1(Cyp2b1) and uridine diphosphate-5¢- glucuronosyltransferase (Ugt1a1)) and transporters including bile salt export pump (Bsep) and multidrug resistance protein 2 (Mdr2)) as well as renal organic solute transporter beta (Ostβ), accompanied by further increase in urinary and biliary excretion of bile acid and bilirubin.Conclusions: DNts might promote bile acid and bilirubin elimination by regulating the expressions of hepatic and renal transporters as well as hepatic metabolic enzymes. © 2014 Ding et al.; licensee BioMed Central Ltd.


Zhou Q.,Shanghai University of Traditional Chinese Medicine | Zhou Q.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Wei X.,Shanghai University of Traditional Chinese Medicine | Wei X.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | And 6 more authors.
Carbohydrate Polymers | Year: 2013

This study investigates the solubility of baicalein (Ba) with the addition of modified cyclodextrins using phase solubility method. The solubility of Ba in the presence of natural (α-, β-, and γ-) cyclodextrins and its derivatives, namely, hydroxypropyl-β-cyclodextrin (HP-β-CD) and (2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD), was higher than that of free Ba. In particular, the stability constant of inclusion complex with DM-β-CD was 13672.67 l mol-1, which was the highest among the examined cyclodextrins. The inclusion complexes of Ba and DM-β-CD were prepared via freeze-drying method, which were both characterized in the solution and solid state by UV-vis spectroscopy, differential scanning calorimetry (DSC), proton nuclear magnetic resonance (1H NMR), scanning electron microscopy (SEM), and X-ray powder diffractometry (XRPD). The UV-vis, DSC, 1H NMR, SEM, and XRPD results proved the formation of inclusion complex between Ba and DM-β-CD. Furthermore, the dissolution rate and thermal stability of the inclusion complex were significantly enhanced compared with the pure drug. Therefore, using DM-β-CD can effectively improve the solubility and thermal stability of free Ba, which is a promising approach to promote its clinical application. © 2013 Elsevier Ltd.


Huang L.-F.,Shanghai University of Traditional Chinese Medicine | Huang L.-F.,China Pharmaceutical University | Shi H.-L.,Shanghai University of Traditional Chinese Medicine | Gao B.,Shanghai University of Traditional Chinese Medicine | And 7 more authors.
Thrombosis Research | Year: 2014

Introduction Dencichine, one of the non-protein amino acids present in the roots of Panax notoginseng, has been found to shorten bleeding time of mice and increase the number of platelets. However, the exact underlying mechanisms have not been elucidated yet. This study was aimed to identify the hemostatic effect of dencichine and uncover its mechanisms. Materials and methods Hemostatic effect was assessed by measuring tail bleeding time and coagulation indices of rats. PT, APTT, TT and FIB concentration were measured using a Sysmex CA-1500 plasma coagulation analyzer. Platelet aggregation rate was determined by using a platelet aggregometer. Concentration of cyotosolic calcium was evaluated by Fluo-3 and levels of cyclic adenosine monophosphate (cAMP) and thromboxane A2 (TXA2) were measured by ELISA method. Results and conclusion Dencichine administered orally shortened tail bleeding time, reduced APTT and TT but increased the concentration of FIB in plasma in a dose-dependent manner. When induced with trap, dencichine could elevate the cytoplasmic concentration of calcium, and secretion of TXA2 as well as the ratio of TXA2 to PGI2 from platelets. Meanwhile, it decreased the level of intracellular cAMP. However, CNQX could block the enhanced hemostatic effect of dencichine. These results suggested that dencichine exerted hemostatic function via AMPA receptors on platelets, therefore, facilitated coagulation cascade in a paracrine fashion by control of platelet cytosolic calcium influx, cAMP production and TXA2 release. Current study may contribute to its clinical use in therapy of hemorrhage. © 2014 Elsevier Ltd. All rights reserved.


Geng F.,Shanghai University of Traditional Chinese Medicine | He Y.,Shanghai University of Traditional Chinese Medicine | Yang L.,Shanghai University of Traditional Chinese Medicine | Wang Z.,Shanghai University of Traditional Chinese Medicine | Wang Z.,Shanghai R and nter for Standardization of Traditional Chinese Medicines
Biomedical Chromatography | Year: 2010

Angiotensin-converting enzyme (ACE) plays an important role in the renin-angiotensin system and ACE activity is usually assayed in vitro by monitoring the transformation from a substrate to the product catalyzed by ACE. A rapid and sensitive analysis method or ACE activity by quantifying simultaneously the substrate hippuryl-histidyl-leucine and its product hippuric acid using an ultra-performance liquid chromatography coupled with electrospray ionization-mass spectrometry (UPLC-MS) was fi rst developed and applied to assay the inhibitory activities against ACE of several natural phenolic compounds. The established UPLC-MS method showed obvious advantages over the conventional HPLC analysis in shortened running time (3.5 min), lower limit of detection (5 pg) and limit of quantifi cation (18 pg), and high selectivity aided by MS detection in selected ion monitoring (SIM) mode. Among the six natural products screened, five compounds, caffeic acid, caffeoyl acetate, ferulic acid, chlorogenic acid and resveratrol indicated potent in vitro ACE inhibitory activity with IC50 values of 2.527 ± 0.032, 3.129 ± 0.016, 10.898 ± 0.430, 15.076 ± 1.211 and 6.359 ± 0.086 mM, respectively. A structure-activity relationship estimation suggested that the number and the situation of the hydroxyls on the benzene rings and the acrylic acid groups may play the most predominant role in their ACE inhibitory activity. Copyright © 2009 John Wiley & Sons, Ltd.

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