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Geng F.,Shanghai University of Traditional Chinese Medicine | He Y.,Shanghai University of Traditional Chinese Medicine | Yang L.,Shanghai University of Traditional Chinese Medicine | Wang Z.,Shanghai University of Traditional Chinese Medicine | Wang Z.,Shanghai R and nter for Standardization of Traditional Chinese Medicines
Biomedical Chromatography | Year: 2010

Angiotensin-converting enzyme (ACE) plays an important role in the renin-angiotensin system and ACE activity is usually assayed in vitro by monitoring the transformation from a substrate to the product catalyzed by ACE. A rapid and sensitive analysis method or ACE activity by quantifying simultaneously the substrate hippuryl-histidyl-leucine and its product hippuric acid using an ultra-performance liquid chromatography coupled with electrospray ionization-mass spectrometry (UPLC-MS) was fi rst developed and applied to assay the inhibitory activities against ACE of several natural phenolic compounds. The established UPLC-MS method showed obvious advantages over the conventional HPLC analysis in shortened running time (3.5 min), lower limit of detection (5 pg) and limit of quantifi cation (18 pg), and high selectivity aided by MS detection in selected ion monitoring (SIM) mode. Among the six natural products screened, five compounds, caffeic acid, caffeoyl acetate, ferulic acid, chlorogenic acid and resveratrol indicated potent in vitro ACE inhibitory activity with IC50 values of 2.527 ± 0.032, 3.129 ± 0.016, 10.898 ± 0.430, 15.076 ± 1.211 and 6.359 ± 0.086 mM, respectively. A structure-activity relationship estimation suggested that the number and the situation of the hydroxyls on the benzene rings and the acrylic acid groups may play the most predominant role in their ACE inhibitory activity. Copyright © 2009 John Wiley & Sons, Ltd.

Huang L.-F.,Shanghai University of Traditional Chinese Medicine | Huang L.-F.,China Pharmaceutical University | Shi H.-L.,Shanghai University of Traditional Chinese Medicine | Gao B.,Shanghai University of Traditional Chinese Medicine | And 7 more authors.
Thrombosis Research | Year: 2014

Introduction Dencichine, one of the non-protein amino acids present in the roots of Panax notoginseng, has been found to shorten bleeding time of mice and increase the number of platelets. However, the exact underlying mechanisms have not been elucidated yet. This study was aimed to identify the hemostatic effect of dencichine and uncover its mechanisms. Materials and methods Hemostatic effect was assessed by measuring tail bleeding time and coagulation indices of rats. PT, APTT, TT and FIB concentration were measured using a Sysmex CA-1500 plasma coagulation analyzer. Platelet aggregation rate was determined by using a platelet aggregometer. Concentration of cyotosolic calcium was evaluated by Fluo-3 and levels of cyclic adenosine monophosphate (cAMP) and thromboxane A2 (TXA2) were measured by ELISA method. Results and conclusion Dencichine administered orally shortened tail bleeding time, reduced APTT and TT but increased the concentration of FIB in plasma in a dose-dependent manner. When induced with trap, dencichine could elevate the cytoplasmic concentration of calcium, and secretion of TXA2 as well as the ratio of TXA2 to PGI2 from platelets. Meanwhile, it decreased the level of intracellular cAMP. However, CNQX could block the enhanced hemostatic effect of dencichine. These results suggested that dencichine exerted hemostatic function via AMPA receptors on platelets, therefore, facilitated coagulation cascade in a paracrine fashion by control of platelet cytosolic calcium influx, cAMP production and TXA2 release. Current study may contribute to its clinical use in therapy of hemorrhage. © 2014 Elsevier Ltd. All rights reserved.

Huang S.,Shanghai University | Yang Q.,Shanghai University | Shi Y.,Shanghai University | Wang R.,Shanghai University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining paeoniflorin and albiflorin in Paeoniae Radix Alba Method: Using paeoniflorin as the internal reference substance, the relative correction factor (RCF) of albiflorin was determined by HPLC and UPLC with good reproducibility. The contents of paeoniflorin in 16 samples of Paeoniae Radix Alba were authentically determined by the external standard method, and the content of albiflorin was calculated according to the RCF. The contents of these two components in the samples were determined with the external standard method. Result: No significant differences between the quantitative results of QAMS method and external standard method were observe. Conclusion: It is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable. It can be used to control the quality of Paeoniae Radix Alba.

Wang R.,Shanghai University | Wang R.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Huang S.,Shanghai University | Yang Q.,Shanghai University | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To establish a HPLC method of characteristic chromatographic profile for the quality control of Paeoniae Radix. Method: The 67 batches of samples were analyzed on a Polaris C18-A column with a gradient elution of acetonitrile and phosphate solution (pH 3.0) at a flow rate of 1.0 mL·min-1 and detected at 230 nm. Result: Nine main marker peaks were identified and semi-quantificated. By the similarity evaluation software for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) and hierarchical clustering analysis, 67 batches of Paeoniae Radix were classified. Conclusion: The method can be applied for quality assessment of Paeoniae Radix.

Wu L.-H.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Wu L.-H.,Shanghai University of Traditional Chinese Medicine | Leon C.J.,Jodrell Laboratory | Wang Z.-T.,Shanghai R and nter for Standardization of Traditional Chinese Medicines | Wang Z.-T.,Shanghai University of Traditional Chinese Medicine
Annales Botanici Fennici | Year: 2012

Gentiana zhenxiongensis L.H. Wu & Z.T. Wang sp. nova (Gentianaceae) from Yunnan province, China, is described and illustrated. It resembles G. praticola, but differs in having stolons and/or rhizomes, small flowers, calyx lobes with keeled midribs, a campanulate corolla and a tuberculate testa. © Finnish Zoological and Botanical Publishing Board 2012.

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