Time filter

Source Type

Li J.,Tongji University | Guo L.,Shanghai Ninth Peoples Hospital | Ai Z.,Tongji University
Future Oncology | Year: 2017

Aim: This study was performed to detect driver genes and implement integrated analyses on these drivers in clear cell renal cell carcinoma (ccRCC). Methods: Driver genes and pathways were predicted by OncodriveFM and Dendrix using 39,636 somatic mutations from The Cancer Genome Atlas, followed by DNA methylation, copy number variation, differential expression and survival analyses. Results: Overall, 342 driver genes and 106 pathways were determined by OncodriveFM, two driver genes by Dendrix. 28 driver genes were found hypomethylated, overexpressed and associated to a poor prognosis. By contrast, 17 driver genes showed decreased expression, hypermethylation and indicated a better outcome in ccRCC. Conclusion: The set of new cancer genes and pathways opens the avenue for developing potential therapeutic targets and prognostic biomarkers in ccRCC. © 2017 Future Medicine Ltd.


Peng Z.-X.,Shanghai Ninth Peoples Hospital | Wang L.,Shanghai JiaoTong University | Du L.,Shanghai Ninth Peoples Hospital | Guo S.-R.,Shanghai JiaoTong University | And 2 more authors.
Carbohydrate Polymers | Year: 2010

Hydroxypropyltrimethyl ammonium chloride chitosan (HACC) was synthesised with differing degrees of substitution (6%, 18% and 44%) of quaternary ammonium by reacting chitosan with glycidyl trimethylammonium chloride. The antibacterial activities of these polymers were tested in vitro against Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, and Staphylococcus epidermidis. Mouse fibroblasts and bone marrow derived stromal cells (hMSCs) were used to investigate the biocompatibility of the HACC. The results show that the antibacterial activities of the HACC with 18% or 44% substitution were significantly higher than the others (P < 0.05) against all three bacteria. HACC with 6% or 18% substitution was not cytotoxic and did not interfere with the proliferation and osteogenic differentiation of hMSCs. Overall, we can make a conclusion that HACC with an 18% substitution was a potential pharmaceutical that can inhibit the growth of bacteria and has good biocompatibility with osteogenic cells. © 2010 Elsevier Ltd. All rights reserved.


Ai S.,Shanghai JiaoTong University | Fan X.,Shanghai JiaoTong University | Fan L.,Shanghai JiaoTong University | Sun Q.,Shanghai JiaoTong University | And 3 more authors.
Carbohydrate Polymers | Year: 2013

In the present study, the Angelica sinensis polysaccharides (ASP) extraction procedure was optimized by an L9 (34) orthogonal array experimental design (OAD) with four factors at three levels. Under the optimal extraction condition (extraction time 180 min, ratio of water to solid 6, extraction temperature 100 °C, and extraction number 4), extraction yield of ASP was 5.6%. Rabbits were fed for 40 days with A. sinensis polysaccharides at a dose of 150 or 300 mg/kg body weight, respectively. At the end of 40 days, animals received cerebral ischemia reperfusion operation. CT perfusion imaging (CTP) analysis showed that rCBF and rCBV were significantly increased, whereas rMTT and rTTP were decreased in the ischemia cerebral tissue compared to CIR group rabbits. ASP significantly decreased oxidative damage, and increased antioxidant enzymes activities in brains of CIR animals. Moreover, ASP significantly enhanced the Ach, Na+,K+-ATPase, Ca 2+,Mg2+-ATPase and glucose levels, decreased AChE activity in brain tissue of the experimental animals. These results suggest a potent role of ASP in protection of brain oxidative injury in CIR animals. © 2013 Elsevier Ltd.


Zuo C.,Shanghai JiaoTong University | Huang Y.,Shanghai Ninth Peoples Hospital | Bajis R.,University of Western Australia | Sahih M.,University of Western Australia | And 5 more authors.
Osteoporosis International | Year: 2012

Bone remodeling is essential for adult bone homeostasis. The failure of this process often leads to the development of osteoporosis, a present major global health concern. The most important factor that affects normal bone remodeling is the tightly controlled and orchestrated regulation of osteoblasts and osteoclasts. The present review summarized the recent discoveries related to osteoblast regulation from several signals, including transforming growth factor-β, bone morphogenetic proteins, Wnt signal, Notch, Eph-Ephrin interaction, parathyroid hormone/parathyroid hormone-related peptide, and the leptin-serotonin-sympathetic nervous systemic pathway. The awareness of these mechanisms will facilitate further research that explores bone remodeling and osteoporosis. Future investigations on the endogenous regulation of osteoblastogenesis will increase the current knowledge required for the development of potential drug targets in the treatment of osteoporosis. © International Osteoporosis Foundation and National Osteoporosis Foundation 2012.


Liu Y.,Shanghai Fuhua Plastic and Aesthetic Hospital | Lei M.,Shenzhen Second Peoples Hospital | Wang Y.,Shanghai Ninth Peoples Hospital | Mu X.,Fudan University
Aesthetic Plastic Surgery | Year: 2012

Background: Ethnic characteristics of the Asian upper eyelid include the lack of a superior palpebral fold, excessive fat, laxity of pretarsal skin, and a medial epi-canthal fold. We present the lazy S-curve epicanthoplasty for correction of the medial epicanthal fold. Methods: The lazy S-curve incision was made as an entrance step. Then the connective and orbicularis oculi muscle dense fibers underlying the epicanthal folds were released and shifted. To reduce the tension of the new medial canthus, we anchored the canthus by suture fixation of both the subdermal and medial orbital periosteum. Rotation of the S-curve flap was settled as the last step. Results: From December 2005 to December 2009, the authors corrected the epicanthal folds on the eyelids of 156 Asian patients using the lazy S-curve epicanthoplasty. Results were satisfactory in most of the follow-up cases. Conclusion: Lazy S-curve epicanthoplasty is a reliable and effective method to eliminate not only the epicanthal fold but also the undesirable orbicularis oculi muscle. © Springer Science+Business Media, LLC and International Society of Aesthetic Plastic Surgery 2011.


Chen H.,East China Normal University | Huang J.,East China Normal University | Huang J.,Wuhan University of Technology | Yu J.,East China Normal University | And 2 more authors.
International Journal of Biological Macromolecules | Year: 2011

This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan- graft-poly (e{open}-caprolactone) (CS- g-PCL) was synthesized with a facile one-step manner by grafting e{open}-caprolactone oligomers onto the hydroxyl groups of CS via ring-opening polymerization by using methanesulfonic acid as solvent and catalyst. And then, CS- g-PCL/PCL nanofibrous mats were obtained by electrospinning of CS- g-PCL/PCL mixed solution. Scanning electron microscopy (SEM) images showed that the morphologies and diameters of the nanofibers were mainly affected by the weight ratio of CS- g-PCL to PCL. The enrichment of amino groups on the nanofiber surface was confirmed by X-ray photoelectron spectroscopy (XPS). With the increase of CS- g-PCL in CS- g-PCL/PCL nanofiber, the content of amino groups on the nanofiber surface increased, which resulted in the increase of zeta-potential of nanofibers. Studies on cell-scaffold interaction were carried out by culturing mouse fibroblast cells (L929) on CS- g-PCL/PCL nanofibrous mats with various contents of CS-g-PCL by assessing the growth, proliferation and morphologies of cells. The results of MTS assay and SEM observation showed that CS- g-PCL/PCL (2/8) mats with a moderate surface zeta-potential (ζ= 3 mV) were the best in promoting the cell attachment and proliferation. Toluidine blue staining further confirmed that L929 cells grew well and exhibited a normal morphology on the CS- g-PCL/PCL (2/8) mats. These results suggested the potential utilization of CS- g-PCL/PCL (2/8) nanofibrous mats for skin tissue engineering. © 2010 Elsevier B.V.


Yue B.,Harvard University | Yue B.,Shanghai Ninth Peoples Hospital | Varadarajan K.M.,Harvard University | Rubash H.E.,Harvard University | Li G.,Harvard University
International Orthopaedics | Year: 2012

Purpose: The object of this study was to investigate the in vivo function of the posterior cruciate ligament (PCL) in patients before and after a PCL-retaining total knee arthroplasty (TKA). Methods: Eleven patients with advanced osteoarthritis (OA) of the knee were recruited. Magnetic resonance scans of each OA knee were obtained, and 3D computer models, including the femoral and tibial insertion areas of the anterolateral and posteromedial bundles of the PCL, were created. Before and after PCL-retaining TKA, dual fluoroscopic images of each knee were acquired during weight-bearing knee flexion. The images and computer models were used to reproduce the in vivo motion of the knee. The function of the PCL bundles was described in terms of elongation, elevation and deviation. Twenty-two healthy controls were also included as normal references. Results: PCL bundles of the OA knees were overstretched during late knee flexion and orientated more medially throughout flexion compared with normal knees. After PCL-retaining TKA, PCL bundles were further overstretched during late flexion and changed from medially directed in normal and OA knees to almost sagittally directed, which may compromise function in controlling knee rotation. Conclusions: The current PCL-retaining TKA systems and surgical techniques may not adequately re-establish normal biomechanics of PCL bundles after PCL-retaining TKA. © Springer-Verlag 2012.


Gu Y.-X.,Shanghai Ninth Peoples Hospital | Du J.,Shanghai Ninth Peoples Hospital | Si M.-S.,Shanghai Ninth Peoples Hospital | Mo J.-J.,Shanghai Ninth Peoples Hospital | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2013

Chemical modification to produce a hydrophilic microrough titanium (Ti) implant surface has been shown to increase osseointegration compared with microrough topography alone. This study aimed to investigate the roles of PI3K/Akt signaling pathway in regulating proliferation and differentiation of osteoblasts in response to surface microroughness and hydrophilicity. Ti disks were manufactured to present different surface morphologies: a smooth pretreatment surface (PT), a rough hydrophobic surface that was sand-blasted, large-grit, acid-etched (SLA), and an SLA surface with the same roughness that was chemically modified to possess high wettability/hydrophilicity (SLActive/modSLA). MC3T3-E1 cells were cultured on these substrates with or without LY294002, a PI3K inhibitor, and their behaviors, including cell viability (MTT colorimetric assay), alkaline phosphatase (ALP) activity, and osteogenic genes expression of osteopontin (OPN) and osteocalcin (OCN) were measured. Western blot was applied to detect the expression of PI3K/Akt signal pathway proteins. The results showed that a decrease in osteoblast proliferation associated with the Ti surfaces (SLActive > SLA > PT) correlated with an increase in activity of the osteogenic differentiation markers ALP. The peak of ALP activity appeared earlier at 7 days for the SLActive surfaces compared with the SLA and PT surfaces. Osteoblast proliferation, as well as the level of p-Akt, was significantly inhibited by LY294002 in all three Ti surfaces. The top value of ALP activity was increased with the inhibition of PI3K/Akt signaling pathway while the time of the peak appeared was not advanced. The expression levels of OPN and OCN were upregulated by the effect of surface roughness and hydrophilicity, which were further enhanced by LY294002. In conclusion, osteogenic responses to SLActive surface were moderately better than the SLA surface and protein expression studies indicated that PI3K/Akt signaling activation may be responsible for this increased osteogenic differentiation. Surface microroughness and hydrophilicity may affect osteoblast functions by targeting osteoblast proliferation and the early stage of osteoblast differentiation through PI3K/Akt signaling pathway. © 2012 Wiley Periodicals, Inc.


Kuang Y.,Shanghai Ninth Peoples Hospital | Hong Q.,Shanghai Ninth Peoples Hospital | Chen Q.,Shanghai Ninth Peoples Hospital | Lyu Q.,Shanghai Ninth Peoples Hospital | And 3 more authors.
Fertility and Sterility | Year: 2014

Objective To explore the feasibility of luteal-phase ovarian stimulation using hMG and letrozole in terms of ovarian response and pregnancy outcome using frozen-thawed embryo transfer. Design A prospective cohort study. Setting Academic tertiary-care medical center. Patient(s) Two hundred forty-two female patients undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment. Intervention(s) Ovarian stimulation was initiated with hMG 225 IU and letrozole 2.5 mg daily after spontaneous ovulation. Letrozole administration was stopped when the dominant follicles reached diameters of 12 mm. Ovulation was induced with a GnRH agonist 100 μg when at least three follicles reached diameters of 18 mm or one dominant follicle reached 20 mm. The highest quality embryos were extracted and cryopreserved for later transfer. Main Outcome Measure(s) The primary outcome measured was the number of oocytes retrieved. Secondary outcomes were the clinical pregnancy rate, ongoing pregnancy rate, and implantation rate after frozen embryo transfer (FET) cycles. Result(s) Of the 242 women enrolled in the study, all participants succeeded in producing oocytes and 227 women had highest-quality embryos to cryopreserve. The average number of oocytes retrieved was 13.1, producing an average of 4.8 highest quality embryos. Moreover, no cases experienced a premature LH surge or moderate/severe ovarian hyperstimulation syndrome during the stimulation cycles. In FETs, the clinical pregnancy rate, ongoing pregnancy rate, and implantation rate were 55.46% (127/229), 48.91% (112/229), and 40.37% (174/431), respectively. Of all the pregnancies in the study, 68 resulted in live births and 44 were ongoing. Conclusion(s) Luteal-phase ovarian stimulation is feasible for producing competent oocytes/embryos in women undergoing IVF/ICSI treatments, with optimal pregnancy outcomes in FET cycles. © 2014 by American Society for Reproductive Medicine.


Chen M.J.,Shanghai Ninth Peoples Hospital | Yang C.,Shanghai Ninth Peoples Hospital | Zhang S.Y.,Shanghai Ninth Peoples Hospital | Cai X.Y.,Shanghai Ninth Peoples Hospital
Journal of Oral and Maxillofacial Surgery | Year: 2010

Purpose: To evaluate the effectiveness of Coblation in temporomandibular joint (TMJ) arthroscopic surgeries and to preliminarily evaluate the clinical effects. Materials and Methods: Arthroscopic Coblation, combined with disc suturing, was performed in 419 TMJs from July 2001 to March 2007 by use of the ArthroCare System 2000 radiofrequency machine (ArthroCare, Sunnyvale, CA). All patients had stage II to V internal derangement, according to the classification of Wilkes. Besides disc suturing, the technique of Coblation was used in procedures of adhesion ablation, anterior release, chondroplasty, and discoplasty. All patients were followed up for 3 months and evaluated based on immediate arthroscopic findings, by clinical examination, and by magnetic resonance imaging examination. Results: During the TMJ arthroscopic surgeries, the adhesion, unhealthy disc, and cartilage were ablated completely. The surface of the cartilage and disc were smooth without cautery damage and hemorrhage. During the clinical follow-up period, 76.37% of TMJs (320/419) had excellent results and 16.47% (69/419) had good results. The success rate was 92.84%. Masseter muscle atrophy occurred in 4 patients, and 30 TMJs required second arthroscopic surgeries or open surgeries. Conclusions: The technique of Coblation has proved to be an effective and minimally invasive option for the treatment of TMJ internal derangement, with advantages such as offering a high degree of precision and control, causing little or no thermal damage to surrounding tissue, leaving smooth anatomic surfaces, and achieving hemostasis of smaller blood vessels. © 2010 American Association of Oral and Maxillofacial Surgeons.

Loading Shanghai Ninth Peoples Hospital collaborators
Loading Shanghai Ninth Peoples Hospital collaborators