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Cheng J.,Shanghai JiaoTong University | Pi S.,Shanghai JiaoTong University | Ye S.,State Oceanic Administration | Gao H.,Shanghai Municipal Oceanic Bureau | And 4 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2012

The current testing for paralytic shellfish poisoning (PSP) in shellfish is based on the mouse bioassay (MBA). To alleviate animal welfare concerns, we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60, 90, and 120 min after injection. Acetylcholine (ACh) was the most sensitive marker for PSP toxicity. The changes in neurotransmitter levels were most pronounced in the blood. Thus, measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice. This method was relatively simple, sensitive (less than 1 μg/kg weight, equivalent to 20 ng/mL), low maintenance, and rapid (less than 60 min). © 2012 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.

Tang Q.-L.,Shanghai JiaoTong University | Cheng J.-P.,Shanghai JiaoTong University | Gao H.-M.,Shanghai Municipal Oceanic Bureau | Yao L.,Shanghai Municipal Oceanic Bureau | And 7 more authors.
Huanjing Kexue/Environmental Science | Year: 2013

In order to discuss the potential ecological risk of heavy metals of the typical dredged mud in Shanghai, the Hakanson potential ecological risks method was used to analyse and assess the potential ecological risks of heavy metals, including Hg, Cd, Cu, Pb, As, Cr and Zn in dredged mud from the following three areas-the dock apron of Huangpu River, the mouth of the Yangtze River and inland waterways. The results showed that the mean values of ecological risk index (Er i) of the seven heavy metals are 20.05, 17.49, 8.82, 5.71, 4.68, 1.74 and 1.13, respectively, all of which belonged to the low ecological risk; Cd(one location in inland waterways) and Hg(three locations in the mouth of the Yangtze River and one location in inland waterways) are the most hazardous elements, with the Er i>40, which belonged to the medium ecological risk or the high ecological risk, and other elements belonged to the low ecological risk. From the results of ecological risk indices(ERI) of the heavy metals in Shanghai dredged mud, the risk of the heavy metals belonged to the low ecological risk. The ERI of inland waterways, the mouth of the Yangtze River and the dock apron of the Huangpu River were 81.4, 57.7 and 52.5, respectively, which all belong to the low ecological risk.

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