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Cheng B.,Shanghai University of Traditional Chinese Medicine | Li J.,Shanghai University of Traditional Chinese Medicine | Du J.,Shanghai University of Traditional Chinese Medicine | Lv X.,Shanghai Municipal Hospital of Traditional Chinese Medicine | And 2 more authors.
Food and Chemical Toxicology

Ginsenosides (GSS), the main active components of ginseng, have been reported possessing anti-osteoporosis activity in ovariectomized rats. However, the active ingredient and the mechanisms underlying the anti-osteoporosis activity of GSS have not been clearly elucidated. In the present study, we determined the effect of ginsenoside Rb1, a major component of ginsenosides, on receptor activator of NF-κB ligand (RANKL)-induced osteoclast formation. Ginsenoside Rb1 inhibited RANKL-induced osteoclast differentiation from Raw264.7 cells without cytotoxicity. Ginsenoside Rb1 also inhibited RANKL-induced TNFα mRNA expression in Raw264.7 cells. Pretreatment with ginsenoside Rb1 significantly inhibited RANKL-induced the gene expression of c-Fos and nuclear factor of activated T-cells c1 (NFATc1), which are two essential and crucial transcription factors for osteoclast formation. Rb1 inhibited RANKL-induced nucleus translocation and activation of NF-κB, the upstream factor of c-Fos and NFATc1. Among the three well known mitogen-activated protein kinases (MAPKs), Rb1 inhibited RANKL-induced JNK and p38 phosphorylation, but not ERK1/2. Taken together, our data suggest that ginsenoside Rb1 is one of the effective components of GSS for the anti-osteoporosis activity and can inhibit osteoclastogenesis by suppressing RANKL-induced activation of both JNK and p38 MAPKs and NF-κB pathways, and consequently down-regulating the gene expression of c-Fos and NFATc1 in osteoclast precursors. © 2012. Source

Gu C.,Shanghai University of Traditional Chinese Medicine | Shen T.,Shanghai Municipal Hospital of Traditional Chinese Medicine
International Journal of Molecular Medicine

In this study, gene expression profiles in peripheral blood monocytes from patients with Alzheimer's disease (AD) or mild cognitive impairment (MCI) were compared with those of healthy individuals to identify key differentially expressed genes (DEGs), in an effort to broaden our understanding of the pathogenesis of these diseases and identify potential therapeutic targets. The microarray data set GSE18309 was downloaded from Gene Expression Omnibus, including 3 AD, 3 MCI and 3 normal control (NC) samples. Raw data were processed and differential analysis was performed using the R multtest package. Two groups of comparisons (AD vs. NC and MCI vs. NC) were conducted and two groups of DEGs were acquired. The common DEGs were selected, for which functional enrichment analysis, as well as pathway enrichment analysis were performed to determine their roles in the development of the diseases in question. A total of 405 DEGs were identified in the AD vs. NC samples and 395 in the MCI vs. NC samples. A total of 60 common DEGs were obtained. Functional enrichment analysis revealed that the most common functions of the DEGs identified were response to nutrients, muscle contraction and cellular homeostasis. As shown by pathway enrichment analysis, the most common pathway associated with the DEGs identifed was the neuroactive ligand-receptor interaction pathway. A range of DEGs was identified in the present study, which may help to disclose the molecular mechanisms responsible for AD and may thus provide potential novel therapeutic strategies for Ad. Source

Ji Q.,Shanghai University of Traditional Chinese Medicine | Liu X.,Shanghai University of Traditional Chinese Medicine | Fu X.,Shanghai University of Traditional Chinese Medicine | Zhang L.,Shanghai University of Traditional Chinese Medicine | And 7 more authors.

Resveratrol, extracted from Chinese herbal medicine Polygonum cuspidatum, is known to inhibit invasion and metastasis of human colorectal cancer (CRC), in which long non-coding Metastasis Associated Lung Adenocarcinoma Transcript 1 (RNA-MALAT1) also plays an important role. Using MALAT1 lentiviral shRNA and over-expression constructs in CRC derived cell lines, LoVo and HCT116, we demonstrated that the anti-tumor effects of resveratrol on CRC are through inhibiting Wnt/β-catenin signaling, thus the expression of its target genes such as c-Myc, MMP-7, as well as the expression of MALAT1. In detail, resveratrol down-regulates MALAT1, resulting in decreased nuclear localization of β-catenin thus attenuated Wnt/β-catenin signaling, which leads to the inhibition of CRC invasion and metastasis. This finding of ours surely provides important pre-clinical evidence supporting future use of resveratrol in prevention and treatment of CRC. Source

Deng Y.,Shanghai JiaoTong University | Zhang Y.,Shanghai JiaoTong University | Sun S.,Shanghai JiaoTong University | Wang Z.,Shanghai JiaoTong University | And 7 more authors.
Scientific Reports

Genetic and transcriptional profiling, as well as surface marker identification of single circulating tumor cells (CTCs) have been demonstrated. However, quantitatively profiling of functional proteins at singleCTC resolution has not yet been achieved, owing to the limited purity of the isolated CTC populations and a lack of single-cell proteomic approaches to handle and analyze rare CTCs. Here, we develop an integrated microfluidic system specifically designed for streamlining isolation, purification and single-cell secretomic profiling of CTCs from whole blood. Key to this platform is the use of photocleavable ssDNA-encoded antibody conjugates to enable a highly purified CTC population with <75 'contaminated' blood cells. An enhanced poly-L-lysine barcode pattern is created on the single-cell barcode chip for efficient capture rare CTC cells in microchambers for subsequent secreted protein profiling. This system was extensively evaluated and optimized with EpCAM-positive HCT116 cells seeded into whole blood. Patient blood samples were employed to assess the utility of the system for isolation, purification and single-cell secretion profiling of CTCs. The CTCs present in patient blood samples exhibit highly heterogeneous secretion profile of IL-8 and VEGF. The numbers of secreting CTCs are found not in accordance with CTC enumeration based on immunostaining in the parallel experiments. Source

Cao H.,Shanghai Municipal Hospital of Traditional Chinese Medicine
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation

This paper analyzes and explores the possibilities of digital information-based management realized by equipment department, general services department, supply room and other material flow departments in different hospitals in order to optimize the procedures of information-based asset management. There are various analytical methods of medical supplies business models, providing analytical data for correct decisions made by departments and leaders of hospital and the governing authorities. Source

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