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Man W.,Shanghai Minhang District Central Hospital | Ming D.,Fudan University | Fang D.,Fudan University | Chao L.,Fudan University | Jing C.,Fudan University
Journal of Cellular Biochemistry | Year: 2014

The antioxidant property of dimethyl sulfoxide (DMSO) was formerly attributed to its direct effects. Our former study showed that DMSO is able to induce heme oxygenase-1 (HO-1) expression in endothelial cells, which is a potent antioxidant enzyme. In this study, we hypothesized that the antioxidant effects of DMSO in cardiomyocytes are mediated or partially mediated by increased HO-1 expression. Therefore, we investigated whether DMSO exerts protective effects against H2O2-induced oxidative damage in cardiomyocytes, and whether HO-1 is involved in DMSO-imparted protective effects, and we also explore the underlying mechanism of DMSO-induced HO-1 expression. Our study demonstrated that DMSO pretreatment showed a cytoprotective effect against H2O2-induced oxidative damage (impaired cell viability, increased apopototic cells rate and caspase-3 level, and increased release of LDH and CK) and this process is partially mediated by HO-1 upregulation. Furthermore, our data showed that the activation of p38 MAPK and Nrf2 translocation are involved in the HO-1 upregulation induced by DMSO. This study reports for the first time that the cytoprotective effect of DMSO in cardiomyocytes is partially mediated by HO-1, which may further explain the mechanisms by which DMSO exerts cardioprotection on H 2O2 injury. © 2014 Wiley Periodicals, Inc. Source

Qi M.,Shaoxing University | Lu H.,Tongji University | Zhang Y.,Tongji University | Lv D.,Tongji University | And 2 more authors.
Proceedings - 2014 IEEE Workshop on Advanced Research and Technology in Industry Applications, WARTIA 2014 | Year: 2014

This paper proposes the orthogonal tensor sparse neighborhood preserving embedding algorithm (OTSNPE) for dimension reduction of the high-dimensional matrix data based on the bag of visual word and in combination with the sparse representation. OTSNPE applies sparse coding to local characteristic quantification of data through completion of within-class sparse representation and preserves the supervised local geometrical information effectively. Finally, the experimental result of the real high-dimensional matrix data set verifies the effectiveness of the algorithm. © 2014 IEEE. Source

Zhang S.,Fudan University | Zhai G.,Shanghai Minhang District Central Hospital | Wang J.,Fudan University | Shi W.,Fudan University | And 2 more authors.
Journal of Pediatric Endocrinology and Metabolism | Year: 2015

Background: Low birth weight is associated with an increased risk of adverse outcomes in many diseases in adult life. We investigated the expression of IGF-II and the status of differentially methylated regions (DMR) in small for gestational age (SGA) infants after birth. Methods: Plasma IGF-II, IGF-II receptor (IGF2R), IGF-I, and IGF-binding protein 3 (IGFBP3) levels were measured after birth in 150 newborn infants. These included 30 term appropriate for gestational age (AGA), 30 term SGA, 30 term large for gestational age (LGA), 30 preterm AGA, and 30 preterm SGA infants. H19 and IGF2 mRNA levels were quantified by fluorescence quantitative polymerase chain reaction (PCR). The methylation status of H19 DMR and IGF2 DMR2 was assessed by methylation-specific PCR (MSP). Plasma IGF-II, IGF2R, IGF-I, and IGFBP3 levels were measured by enzyme-linked immunosorbent assay in AGA infants of different gestational ages and term AGA infants on different days. Result: Plasma IGF-II levels after birth were lower in both term SGA (435.1±33.82 vs. 620.4±44.79, p=0.002) and LGA infants (483.7±33.8 vs. 620.42±44.79, p=0.018) than in term AGA infants. The expression of IGF2 mRNA was higher in the term SGA group than in the preterm SGA group (p=0.014) and the preterm SGA group displayed lower expression than the preterm AGA group (p=0.048). The H19 DMR methylation level was higher in both term SGA (p=0.044) and term LGA infants (p=0.027) compared to term AGA infants. Conclusions: IGF-II was associated with birth weight and expressed at high levels, which suggests that IGF-II may continue to play an important role after birth. H19 DMR methylation may be involved in controlling IGF-II expression. © 2015 by De Gruyter. Source

Liang C.,Shanghai Minhang District Central Hospital | Xue Z.,Fudan University | Cang J.,Fudan University | Wang H.,Fudan University | Li P.,Fudan University
Molecular and Cellular Biochemistry | Year: 2011

Dimethyl sulfoxide (DMSO) has recently been proposed as an anti-inflammatory and free radical scavenging agent. However, the mechanisms by which DMSO mediates its therapeutic effects are unclear. In this paper, we investigated the capability of DMSO to up-regulate heme oxygenase-1(HO-1) expression, as well as the possible underlying mechanisms in human umbilical vein endothelial cells (HUVECs). DMSO induced HO-1 expression both at the level of mRNA and protein in dose-and time-dependent manners in HUVECs, resulting in increased HO-1 activity. The pharmacological inhibition of cJun-N-terminal kinases (JNKs) blocked the DMSO-induced HO-1 up-regulation, while inhibition of extracellular regulated kinase and p38-MAPK did not block heme oxygenase-1 up-regulation. In addition, the phosphorylation of JNKs was initiated by DMSO, indicating the involvement of this kinase in the observed response. DMSO increased the nuclear translocation of NF-E2-related factor 2 (Nrf2) and enhanced its binding to the anti-oxidant response element. Inhibition of Nrf2 synthesis by small interfering RNA molecules subsequently inhibited HO-1 expression induced by DMSO, indicating DMSO's role in inducing HO-1 expression via Nrf2 activation. Utilizing these findings, the present study identified DMSO as a novel inducer of HO-1 expression and identified the underlying mechanisms involved in this process. © 2011 Springer Science+Business Media, LLC. Source

Zhang X.,Fudan University | Ding X.,Fudan University | Cao G.,Shanghai JiaoTong University | Deng Y.,Shanghai University of Traditional Chinese Medicine | And 3 more authors.
Current Medical Research and Opinion | Year: 2012

Objective: To evaluate the efficacy and safety of 80 or 160mg/day valsartan in Chinese adult patients with essential hypertension and albuminuria. Methods: A multicenter prospective open-label observational study was conducted. Adult hypertensive patients with albuminuria were treated with 80 or 160mg/day valsartan. Blood pressure (BP) was recorded at weeks 4, 8 and 12. Albuminuria was selectively measured at week 12. Results: The intent-to-treat (ITT) population included 1180 patients. Mean sitting systolic BP (MSSBP) and diastolic BP (MSDBP) at baseline was 153.5 [14.41] (mean [SD]) and 88.2 [11.99] (mean [SD])mmHg. MSSBP and MSDBP at week 4 (mean [SD]: 139.1 [12.02] and 82.2 [8.54]mmHg), week 8 (mean [SD]: 135.3 [10.46] and 80.2 [7.55]mmHg) and week 12 (mean [SD]: 132.1 [9.80] and 78.1 [6.88]mmHg) were significantly lower than baseline (p<0.001). The BP control rate of ITT patients at week 12 was 17.8. Diabetic patients exhibited lower BP control rate than non-diabetic patients (14.0 vs. 22.4, p<0.001). A total of 904 patients had albuminuria assessed at week 12. UACR and UAER at week 12 were lower compared with baseline (p<0.05). In all, 171 patients (18.9) returned to normal albuminuria and albuminuria normalization percentage in diabetic patients exceeded that of non-diabetic patients (26.3 vs. 12.1, p<0.001). Albuminuria declined by more than 50 in 340 patients (37.6) and more diabetic patients exhibited such decline in albuminuria than non-diabetic patients (41.7 vs. 33.8, p<0.05). No serious drug-related adverse effects (AEs) were observed. Limitations: Prior antihypertensive treatment before valsartan administration may interfere with the efficacy of subsequent treatment. The discrepancy between 80 and 160mg may affect patient outcomes and occurrence of AEs. Conclusions: Valsartan can safely and effectively reduce BP and albuminuria in Chinese adult patients with essential hypertension and albuminuria. Valsartan has the more dramatic effect on albuminuria in diabetic patients than non-diabetic patients. © 2012 Informa UK Ltd. Source

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