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Yan Y.-W.,Xinchang Peoples Hospital | Zhou J.-J.,Shanghai Medical Imaging Institute | Zhang L.,Xinchang Peoples Hospital | Hu S.-B.,Xinchang Peoples Hospital
Journal of Practical Oncology

To investigate the relationship of CT findings with pathological results of retroperitoneal leiomyosarcoma. Nine patients with retroperitoneal leiomyosarcoma confirmed by surgical pathology underwent plain scanning and dynamic contrast-enhanced scanning with multi-detector spiral CT (MDCT) before operation. The correlation of CT scan and pathological findings was analyzed. Plain CT scanning showed that the tumor presented oval shape in 7 cases and irregular shape in 2; with rough edges in 5 cases and smooth edges in 4. The average diameter of tumors was 8.3 cm (4.7~14.6 cm). The density of tumors was uneven. Patchy low density necrosis areas were observed in all 9 cases with various sizes and calcification was found in 3 cases. Dynamic CT scanning showed that the parenchyma of tumors presented progressive enhancement and there was no enhancement in necrosis areas. Vascular image was found in 4 cases and with affluent blood vessels in 1 case; the tumors were obscured by common iliac artery in 2 cases. Pathological examination demonstrated ample spindle-shaped tumor cells with significant heteromorphism. The coagulative necrosis foci were observed, and the number of nuclear division was 12~16/10 HPF. Immunohistochemistry revealed SMA (+), Des (+), Vim (+), EMA (+/-) and CEA (-) in tumor cells. Retroperitoneal leiomyosarcoma presents characteristic features on plain and contrast-enhanced CT scans, which may be of value in preoperative diagnosis. © 2015, Editorial Board of Journal of Practical Oncology. All right reserved. Source

Li Y.-S.,Fudan University | Hou K.,Shanghai Medical Imaging Institute | Xu X.,Fudan University | Yang J.,Fudan University | And 5 more authors.
Chinese Medical Journal

Background Although Multi-planar reconstruction (MPR) has been considered a diagnostic imaging technique that observes more perspectives for diseases, few people have applied it surgically. In fact, MPR is also very useful to clinical operation, especially for patients with type B aortic dissection. It helps the surgeon to locate accurately with more information about aortic dissection, so that the safety and effectiveness of operation can be improved. This study examined the application of the MPR in intraoperative DSA imaging for precise positioning by accurately obtaining a cross-section, a spin angle of the coronal plane, and a tilt angle of the sagittal plane in treatment of type B aortic dissection. Methods The conventional and the MPR approaches were compared on positioning the aortic arch for surgery. A group of 40 patients (group A) and another group of 42 patients (group B) was sampled. About the comparison of baseline characteristics, a fourfold table X2 test was conducted on gender, and two independent samples t-test was applied to age between group A and group B. Spin as well as tilt angles for group A were obtained from the patients using both approaches, and their effectiveness was compared with pair t-tests; The MPR data guided stent-grafting in this group. Stent graft placement of group B was based on the conventional approach. Percentages of proximal distributed markers as well as incidences of complications were collected from both groups after stent graft placement. They were also compared with a fourfold table X2 test. Results Gender difference was not found between group A and group B (X2=0.80, P >0.05), and age difference was not statistically significant (F=2.55, homogeneity of variance, t=-1.46, P >0.05). A significant difference was found between the conventional and the MPR approaches for spin angle (t=9.17) as well as tilt angle (t=2.07), P <0.05. Percentage of proximal distributed markers (5.0%) of group A was significantly lower than that of group B (42.9%), X2=15.92, P <0.05; and incidence of complications (5.0%) of group A was also significant lower than that of group B (21.4%), X2=4.76, P <0.05. Conclusions Application of the MPR facilitated intraoperative angle adaption and led to satisfactory DSA. It is feasible in endovascular treatment of type B aortic dissection, and can effectively and safely guide surgical operations. Source

Gu G.,Fudan University | Wan F.,Shanghai Medical Imaging Institute | Wan F.,Fudan University | Xue Y.,Fudan University | And 7 more authors.
Experimental and Therapeutic Medicine

The aim of the present study was to investigate the association between serum lumican levels and acute aortic dissection (AAD) severity. A total of 82 patients with chest or back pain and 30 healthy volunteers were recruited. Among the patients, there were 70 cases of AAD and 12 cases of intramural hematoma (IMH). AAD severity was determined using multi-slice computed tomography angiography (MSCTA). Serum was collected from the patients upon admission, and lumican levels were detected using an enzyme-linked immunosorbent assay. In addition, correlation analyses were conducted between lumican levels and AAD severity by designing a ‘SCORE X, RANGE Y’ system to measure the number of affected vital arteries and vertical range of false lumen, based on the MSCTA. Lumican levels differed significantly among the AAD patients (2.32±4.29 ng/ml), IMH patients (0.72±0.32 ng/ml) and healthy volunteers (0.85±0.53 ng/ml; P=0.003). In the AAD patients presenting within 12-72 h of symptom onset, the Spearman's rho correlation coefficient between lumican and SCORE or RANGE was 0.373 (P=0.046) and 0.468 (P=0.010), respectively. The present results suggest that lumican may be a potential marker for aiding the diagnosis and screening for AAD, and may be used to predict the severity of AAD. © 2016, Spandidos Publications. All Rights reserved. Source

Wang W.-S.,Fudan University | Wang W.-S.,Shanghai Medical Imaging Institute | Liu X.-H.,Fudan University | Liu L.-X.,Fudan University | And 6 more authors.
Journal of Proteomics

Histological differentiation is a major pathological parameter associated with poor prognosis in patients with pancreatic adenocarcinoma (PAC) and the molecular signature underlying PAC differentiation may involve key proteins potentially affecting the malignant characters of PAC. We aimed to identify the proteins which could be implicated in PAC prognosis. We used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to compare protein expression in PAC tissues with different degrees of histological differentiation. A total of 1623 proteins were repeatedly identified by performing the iTRAQ-based experiments twice. Of these, 15 proteins were differentially expressed according to our defined criteria. Myoferlin (MYOF) was selected to validate the proteomic results by western blotting. Immunohistochemistry in a further 154 PAC cases revealed that myoferlin significantly correlated with the degree of histological differentiation (P=0.004), and univariate and multivariate analyses indicated that MYOF is an independent prognostic factor for survival (hazard ratio, 1.540; 95% confidence interval, 1.061-2.234; P=0.023) of patients with PAC after curative surgery. RNA interference-mediated knockdown of MYOF alleviated malignant phenotypes of both primary and metastatic PAC cell lines in vitro and in vivo. Thus, ITRAQ-based quantitative proteomics revealed the prognostic value of MYOF in PAC. Biological significance: Our results provide the possibility of novel strategies for pancreatic adenocarcinoma management. © 2013 Elsevier B.V. Source

Rao S.-X.,Fudan University | Rao S.-X.,Shanghai Medical Imaging Institute | Meng T.,Fudan University | Meng T.,Shanghai Medical Imaging Institute | And 4 more authors.
Acta Radiologica

Background: Volumetric and whole-tumor attenuation assessment of tumor are of value in assessment of treatment. Purpose: To assess the impact of slice thickness on semi-automatic analyses (volume, whole-tumor attenuation) for small colorectal hepatic metastases. Material and Methods: Computed tomography (CT) data of patients with colorectal hepatic metastases at 1.5-, 3-, and 5-mm slice thickness were semi-automatically evaluated for volume and whole-tumor attenuation by two radiologists independently. Statistical analysis included paired samples t-test and concordance correlation coefficient (CCC) analysis according to the longest axial tumor diameter (10-20 mm, 20-30 mm, 30-40 mm). Results: A total of 62 patients (32 men and 30 women) with 62 target tumors were included. The mean volume was significantly higher at 3-and 5-mm slice thicknesses in comparison with the reference (1.5 mm) for the target tumors between 10 mm and 20 mm (P 0.0295, CCC 0.9394 for 3 mm; P 0.0029, CCC 0.5129 for 5 mm, respectively) and at 5 mm slice thickness for the target tumors between 20 mm and 30 mm (P 0.0071, CCC 0.9102). For whole-tumor attenuation measurements, the significant difference was only seen at 5-mm slice thicknesses in comparison with the reference (1.5 mm) for the target tumors between 10 and 20 mm (P 0.0015, CCC 0.9389). Conclusion: Slice thickness of 1.5 mm might be suggested for semi-automated volumetric measurements, and slice thickness of no more than 3 mm for whole-tumor CT attenuation in hepatic metastasis between 10 mm and 20 mm. Source

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