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Gu G.,Fudan University | Wan F.,Shanghai Medical Imaging Institute | Wan F.,Fudan University | Xue Y.,Fudan University | And 7 more authors.
Experimental and Therapeutic Medicine | Year: 2016

The aim of the present study was to investigate the association between serum lumican levels and acute aortic dissection (AAD) severity. A total of 82 patients with chest or back pain and 30 healthy volunteers were recruited. Among the patients, there were 70 cases of AAD and 12 cases of intramural hematoma (IMH). AAD severity was determined using multi-slice computed tomography angiography (MSCTA). Serum was collected from the patients upon admission, and lumican levels were detected using an enzyme-linked immunosorbent assay. In addition, correlation analyses were conducted between lumican levels and AAD severity by designing a ‘SCORE X, RANGE Y’ system to measure the number of affected vital arteries and vertical range of false lumen, based on the MSCTA. Lumican levels differed significantly among the AAD patients (2.32±4.29 ng/ml), IMH patients (0.72±0.32 ng/ml) and healthy volunteers (0.85±0.53 ng/ml; P=0.003). In the AAD patients presenting within 12-72 h of symptom onset, the Spearman's rho correlation coefficient between lumican and SCORE or RANGE was 0.373 (P=0.046) and 0.468 (P=0.010), respectively. The present results suggest that lumican may be a potential marker for aiding the diagnosis and screening for AAD, and may be used to predict the severity of AAD. © 2016, Spandidos Publications. All Rights reserved.


Rao S.-X.,Fudan University | Rao S.-X.,Shanghai Medical Imaging Institute | Zeng M.-S.,Fudan University | Zeng M.-S.,Shanghai Medical Imaging Institute
World Chinese Journal of Digestology | Year: 2016

Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA), a liver-specific magnetic resonance imaging (MRI) contrast agent, is increasingly used for imaging-based liver function tests. Like indocyanine green and mebrofenin, Gd-EOBDTPA is taken up by hepatocytes through organic anion-transporting polypeptides 1 (OATP1) B1 and B3 and is then excreted into the bile by multi-drug resistance protein (MRP2). The advantages of Gd-EOB-DTPA-based liver function tests include function measurement integrated in an existing MRI protocol, ability of evaluating segmental liver function, and no ionizing radiation. The approaches based on Gd-EOB-DTPA for function measurement are as follows: measurement of biliary elimination, hepatic parenchymal enhancement, MR relaxometry, and MR perfusion. These approaches have potential value for assessing liver reserve, hepatic fibrosis, non-alcoholic fatty liver disease and so on. © 2016 Baishideng Publishing Group Inc. All rights reserved.


Wang W.-S.,Fudan University | Wang W.-S.,Shanghai Medical Imaging Institute | Liu X.-H.,Fudan University | Liu L.-X.,Fudan University | And 6 more authors.
Journal of Proteomics | Year: 2013

Histological differentiation is a major pathological parameter associated with poor prognosis in patients with pancreatic adenocarcinoma (PAC) and the molecular signature underlying PAC differentiation may involve key proteins potentially affecting the malignant characters of PAC. We aimed to identify the proteins which could be implicated in PAC prognosis. We used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to compare protein expression in PAC tissues with different degrees of histological differentiation. A total of 1623 proteins were repeatedly identified by performing the iTRAQ-based experiments twice. Of these, 15 proteins were differentially expressed according to our defined criteria. Myoferlin (MYOF) was selected to validate the proteomic results by western blotting. Immunohistochemistry in a further 154 PAC cases revealed that myoferlin significantly correlated with the degree of histological differentiation (P=0.004), and univariate and multivariate analyses indicated that MYOF is an independent prognostic factor for survival (hazard ratio, 1.540; 95% confidence interval, 1.061-2.234; P=0.023) of patients with PAC after curative surgery. RNA interference-mediated knockdown of MYOF alleviated malignant phenotypes of both primary and metastatic PAC cell lines in vitro and in vivo. Thus, ITRAQ-based quantitative proteomics revealed the prognostic value of MYOF in PAC. Biological significance: Our results provide the possibility of novel strategies for pancreatic adenocarcinoma management. © 2013 Elsevier B.V.


Rao S.-X.,Fudan University | Rao S.-X.,Shanghai Medical Imaging Institute | Meng T.,Fudan University | Meng T.,Shanghai Medical Imaging Institute | And 4 more authors.
Acta Radiologica | Year: 2013

Background: Volumetric and whole-tumor attenuation assessment of tumor are of value in assessment of treatment. Purpose: To assess the impact of slice thickness on semi-automatic analyses (volume, whole-tumor attenuation) for small colorectal hepatic metastases. Material and Methods: Computed tomography (CT) data of patients with colorectal hepatic metastases at 1.5-, 3-, and 5-mm slice thickness were semi-automatically evaluated for volume and whole-tumor attenuation by two radiologists independently. Statistical analysis included paired samples t-test and concordance correlation coefficient (CCC) analysis according to the longest axial tumor diameter (10-20 mm, 20-30 mm, 30-40 mm). Results: A total of 62 patients (32 men and 30 women) with 62 target tumors were included. The mean volume was significantly higher at 3-and 5-mm slice thicknesses in comparison with the reference (1.5 mm) for the target tumors between 10 mm and 20 mm (P 0.0295, CCC 0.9394 for 3 mm; P 0.0029, CCC 0.5129 for 5 mm, respectively) and at 5 mm slice thickness for the target tumors between 20 mm and 30 mm (P 0.0071, CCC 0.9102). For whole-tumor attenuation measurements, the significant difference was only seen at 5-mm slice thicknesses in comparison with the reference (1.5 mm) for the target tumors between 10 and 20 mm (P 0.0015, CCC 0.9389). Conclusion: Slice thickness of 1.5 mm might be suggested for semi-automated volumetric measurements, and slice thickness of no more than 3 mm for whole-tumor CT attenuation in hepatic metastasis between 10 mm and 20 mm.


Li Y.-S.,Fudan University | Hou K.,Shanghai Medical Imaging Institute | Xu X.,Fudan University | Yang J.,Fudan University | And 5 more authors.
Chinese Medical Journal | Year: 2013

Background Although Multi-planar reconstruction (MPR) has been considered a diagnostic imaging technique that observes more perspectives for diseases, few people have applied it surgically. In fact, MPR is also very useful to clinical operation, especially for patients with type B aortic dissection. It helps the surgeon to locate accurately with more information about aortic dissection, so that the safety and effectiveness of operation can be improved. This study examined the application of the MPR in intraoperative DSA imaging for precise positioning by accurately obtaining a cross-section, a spin angle of the coronal plane, and a tilt angle of the sagittal plane in treatment of type B aortic dissection. Methods The conventional and the MPR approaches were compared on positioning the aortic arch for surgery. A group of 40 patients (group A) and another group of 42 patients (group B) was sampled. About the comparison of baseline characteristics, a fourfold table X2 test was conducted on gender, and two independent samples t-test was applied to age between group A and group B. Spin as well as tilt angles for group A were obtained from the patients using both approaches, and their effectiveness was compared with pair t-tests; The MPR data guided stent-grafting in this group. Stent graft placement of group B was based on the conventional approach. Percentages of proximal distributed markers as well as incidences of complications were collected from both groups after stent graft placement. They were also compared with a fourfold table X2 test. Results Gender difference was not found between group A and group B (X2=0.80, P >0.05), and age difference was not statistically significant (F=2.55, homogeneity of variance, t=-1.46, P >0.05). A significant difference was found between the conventional and the MPR approaches for spin angle (t=9.17) as well as tilt angle (t=2.07), P <0.05. Percentage of proximal distributed markers (5.0%) of group A was significantly lower than that of group B (42.9%), X2=15.92, P <0.05; and incidence of complications (5.0%) of group A was also significant lower than that of group B (21.4%), X2=4.76, P <0.05. Conclusions Application of the MPR facilitated intraoperative angle adaption and led to satisfactory DSA. It is feasible in endovascular treatment of type B aortic dissection, and can effectively and safely guide surgical operations.


Chen C.-Z.,Fudan University | Chen C.-Z.,Shanghai Medical Imaging Institute | Rao S.-X.,Fudan University | Rao S.-X.,Shanghai Medical Imaging Institute | And 8 more authors.
Hepatology International | Year: 2014

Purpose: To evaluate the differences in enhancement pattern of hepatocellular carcinoma (HCC) 20 mm or smaller and enhancement effects of hepatic vessels on early dynamic contrast-enhanced magnetic resonance imaging (MRI) obtained with gadoxetic acid and gadopentetate dimeglumine in the same patients with cirrhosis. Methods: We reviewed MR images using gadoxetic acid and gadopentetate dimeglumine in the same 34 patients with 42 histologically confirmed HCCs (median diameter, 14.5 mm). The percentage enhancements (PEs) of HCC, the hepatic artery and portal vein and relative contrasts (RCs) between HCC and the liver were calculated and analyzed statistically. Results: The PEs of HCC, the hepatic artery and portal vein were significantly lower for gadoxetic acid in comparison with gadopentetate dimeglumine in the arterial phase (p = 0.0256 for HCC, p < 0.0001 for hepatic artery) and portal phase (p < 0.0001 for HCC, portal vein). The RC between HCC and the liver was significantly lower for gadoxetic acid in comparison with gadopentetate dimeglumine in the arterial phase (p = 0.0422), but was not significantly different in the portal phase (p = 0.1133). Forty-one of the 42 (97.62 %) nodules showed arterial hypervascularization. Of these, 31 (75.61 %) nodules were hypointense in the portal phase for gadoxetic acid, and 22 (53.66 %) were hypointense for gadopentetate dimeglumine (p = 0.038). Conclusions: Compared with gadopentetate dimeglumine, gadoxetic acid-enhanced MRI demonstrated a different enhancement pattern of inferior arterial enhancement and was more rapidly hypointense in the portal phase for HCC. It showed markedly lower enhancement for hepatic artery and portal vein in the patients with cirrhosis. © 2013 Asian Pacific Association for the Study of the Liver.


Huang Y.-Q.,Fudan University | Huang Y.-Q.,Shanghai Medical Imaging Institute | Huang Y.-Q.,Ningbo First Hospital | Liang H.-Y.,Fudan University | And 9 more authors.
Medicine (United States) | Year: 2016

The objective is to explore the value of preoperative magnetic resonance (MR) histogram analyses in predicting microvascular invasion (MVI) of hepatocellular carcinoma (HCC). Fifty-one patients with histologically confirmed HCC who underwent diffusion-weighted and contrast-enhanced MR imaging were included. Histogram analyses were performed and mean, variance, skewness, kurtosis, 1th, 10th, 50th, 90th, and 99th percentiles were derived. Quantitative histogram parameters were compared between HCCs with and without MVI. Receiver operating characteristics (ROC) analyses were generated to compare the diagnostic performance of tumor size, histogram analyses of apparent diffusion coefficient (ADC) maps, and MR enhancement. The mean, 1th, 10th, and 50th percentiles of ADC maps, and the mean, variance. 1th, 10th, 50th, 90th, and 99th percentiles of the portal venous phase (PVP) images were significantly different between the groups with and without MVI (P <0.05), with area under the ROC curves (AUCs) of 0.66 to 0.74 for ADC and 0.76 to 0.88 for PVP. The largest AUC of PVP (1th percentile) showed significantly higher accuracy compared with that of arterial phase (AP) or tumor size (P <0.001). MR histogram analyses - in particular for 1th percentile for PVP images - held promise for prediction of MVI of HCC. © 2016 Wolters Kluwer Health, Inc. All rights reserved.


Shan Y.,Fudan University | Shan Y.,Shanghai Medical Imaging Institute | Lin J.,Fudan University | Lin J.,Shanghai Medical Imaging Institute | And 6 more authors.
Journal of Computer Assisted Tomography | Year: 2012

OBJECTIVES: To investigate the feasibility of one-stop examination of aortic pulse wave velocity (PWV) , aortic distensibility (AD), and brachial artery flow-mediated dilation (FMD) in healthy volunteers by means of 3.0-T high-resolution magnetic resonance imaging (MRI). METHODS: A total of 32 healthy volunteers (24-37 years old; mean age, 29 years) underwent repeated high-resolution MRI to assess aortic PWV, ascending aorta (AA) AD, proximal descending aorta (PDA) AD, distal descending aorta (DDA) AD, and FMD of the brachial artery with an interval of 1 to 2 hours. Pulse wave velocity was evaluated by 2-dimensional (2D) phase contrast velocity-encoded MRI with a 4.7- to 7.8-millisecond (ms) temporal resolution. Fiesta-cine MRI was used to assess AD and FMD with a 18.75- to 31.25-ms temporal resolution. The image quality of repeated scans was evaluated with 4-point scores, and the agreement between the 2 scans was tested with kappa analysis. The reproducibility of the results from repeated measurements for PWV, AA-AD, PDA-AD, DDA-AD, and FMD was assessed with intraclass correlation coefficient (ICC) analysis. The method of Bland-Altman plot was also used to assess the agreement between repeated measurements. RESULTS: Each examination including PWV, AD, and FMD were completed in approximately half an hour. The image quality between repeated scans showed good agreement (κ value, 0.776) with a mean score of 3.53 and 3.41, respectively. Reproducibility between repeated measurements was high for aortic PWV (4.33 ± 0.88 vs 4.36 ± 0.88 m/s; ICCPWV = 0.95; P < 0.01), AA-AD (8.60 ± 3.11 vs 8.59 ± 3.10 × 10 mm Hg; ICCAA-AD = 0.97; P < 0.01), PDA-AD (6.95 ± 2.44 vs 6.95 ± 2.42 × 10 mm Hg; ICCPDA-AD = 0.99; P < 0.01), DDA (10.54 ± 2.91 vs 10.55 ± 2.90 × 10mm Hg; ICCDDA-AD = 0.98, P < 0.01), and FMD (24.94 ± 12.55 vs 24.92 ± 12.38%; ICCFMD = 0.94, P < 0.01). Good agreement between repeated measurements was found for aortic PWV (confidence interval [CI], -0.55 to 0.50), AA-AD (CI, -0.11 to 0.12), PDA-AD (CI, -0.08 to 0.08), DDA-AD (CI, -0.23 to 0.21), and FMD (CI, -1.46 to 1.51). CONCLUSION: Comprehensive assessment of aortic compliance and brachial endothelial function can be achieved in a single examination using 3.0-T high-resolution MRI with good reproducibility and within a reasonable amount of time. Copyright © 2012 by Lippincott Williams & Wilkins.


PubMed | Fudan University and Shanghai Medical Imaging Institute
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

The aim of the present study was to investigate the association between serum lumican levels and acute aortic dissection (AAD) severity. A total of 82 patients with chest or back pain and 30 healthy volunteers were recruited. Among the patients, there were 70 cases of AAD and 12 cases of intramural hematoma (IMH). AAD severity was determined using multi-slice computed tomography angiography (MSCTA). Serum was collected from the patients upon admission, and lumican levels were detected using an enzyme-linked immunosorbent assay. In addition, correlation analyses were conducted between lumican levels and AAD severity by designing a SCORE X, RANGE Y system to measure the number of affected vital arteries and vertical range of false lumen, based on the MSCTA. Lumican levels differed significantly among the AAD patients (2.324.29 ng/ml), IMH patients (0.720.32 ng/ml) and healthy volunteers (0.850.53 ng/ml; P=0.003). In the AAD patients presenting within 12-72 h of symptom onset, the Spearmans rho correlation coefficient between lumican and SCORE or RANGE was 0.373 (P=0.046) and 0.468 (P=0.010), respectively. The present results suggest that lumican may be a potential marker for aiding the diagnosis and screening for AAD, and may be used to predict the severity of AAD.

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