Shanghai Key Laboratory of Veterinary Biotechnology

Shanghai, China

Shanghai Key Laboratory of Veterinary Biotechnology

Shanghai, China
SEARCH FILTERS
Time filter
Source Type

Zhen L.,Shanghai Key Laboratory of Veterinary Biotechnology | Wang L.,Shanghai Key Laboratory of Veterinary Biotechnology | Fu J.,Shanghai Key Laboratory of Veterinary Biotechnology | Li Y.,Shanghai Key Laboratory of Veterinary Biotechnology | And 2 more authors.
Reproductive Toxicology | Year: 2016

Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm motility, protein phosphorylation, mitochondrial membrane potential (δπm) and metabolic enzyme activity starting at 4 μmol/mL following incubation for 1.5 h. Notably, all parameters were potently inhibited by 10 μmol/mL Cr, while supplementation with the dibutyryl-cAMP (dbcAMP) and the 3-isobutyl-1-methylxanthine (IBMX) prevented the inhibition of protein phosphorylation. Interestingly, high concentrations of Cr (>10 μmol/mL) increased the tyrosine phosphorylation of some high-molecular-weight proteins in the principle piece but decreased that in the middle piece associated with an extreme reduction of sperm motility. These results suggest that chromium affects boar sperm motility by impairing tyrosine phosphorylation in the midpiece of sperm by blocking the cAMP/PKA pathway in boar sperm in vitro. © 2015 Elsevier Inc.


Chen X.L.,Shanghai Key Laboratory of Veterinary Biotechnology | Chen X.L.,Jiangxi Academy of Agricultural science | Gong L.Z.,Shanghai Key Laboratory of Veterinary Biotechnology | Xu J.X.,Shanghai Key Laboratory of Veterinary Biotechnology
Animal | Year: 2013

In this study, antioxidant capability and protective effect of probiotics on reproductive damage induced by diet oxidative stress were investigated. Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group. The control group consumed a normal standard diet (5% fat, w/w). The other two treatment groups were fed with a high-fat diet (20% fat, w/w), and a high-fat diet supplemented with 2% probiotics (w/w), respectively. At the end of the experimental period, that is, after 6 weeks, rats were killed. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of nitric oxide (NO) free radical and malondialdehyde (MDA) in serum and sperm suspension were examined. Sperm parameters including sperm concentration, viability, motility and DNA integrity were analyzed. The results showed that high-fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation, NO free radical, significant decrease in activities of SOD, GSH-Px, significant reduction in sperm concentration, viability and motility, and damage in sperm DNA (P < 0.05), compared with the control group. These alterations were significantly reversed in the probiotics-supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and sperm parameters compared with the control group. The percentage of sperm with DNA damage was significantly lower than the high-fat diet group and still higher than the control group, which means that probiotics could attenuate sperm damage to some extent. The present results indicated that dietary probiotics had antioxidant activity and the protective effect against sperm damage induced by high-fat diet to some extent. © The Animal Consortium 2012.


Yuan C.,Shanghai JiaoTong University | Zhu C.,Shanghai Key Laboratory of Veterinary Biotechnology | Wu Y.,Inner Mongolia Agricultural University | Pan X.,Shanghai JiaoTong University | Hua X.,Shanghai JiaoTong University
PLoS Neglected Tropical Diseases | Year: 2011

With the improvements in diagnostic techniques, Bartonella henselae (B. henselae) infection has recently been recognized to cause a widening spectrum of diseases. Cats are the natural reservoir hosts of B. henselae. The current study aims to investigate the prevalence of B. henselae infection in the cat populations in China. Polymerase chain reaction (PCR) and bacterial cultures confirm that 12.7% of the tested cats were positive for the infection. Old age and outdoor exposure were statistically associated with the infection. Multilocus sequence typing and eBURST analysis of the cat isolates collected in the present study show that 65.4% of the isolates belong to sequence type 1 (ST1). Three new STs (ST16-18) were identified in Midwestern China. These results may aid our understanding of the population structure of B. henselae in China and the relationship between human and cat strains in subsequent studies. © 2011 Yuan et al.


Wang Y.-K.,Shanghai Key Laboratory of Veterinary Biotechnology | Yan Y.-X.,Shanghai Key Laboratory of Veterinary Biotechnology | Li S.-Q.,Shanghai Entry Exit Inspection and Quarantine Bureau | Wang H.-A.,Shanghai Key Laboratory of Veterinary Biotechnology | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Mycotoxins produced by different species of fungi may coexist in single cereal and feedstuff samples, which could become highly toxic for humans and animals. In order to quantify four mycotoxins (zearalenone, fumonisin B1, deoxynivalenol, and aflatoxin B1) in cereal and feedstuff samples simultaneously, a new suspension array immunoassay was developed. Antimycotoxin monoclonal antibodies were conjugated to the surface of different encoding microspheres (19#, 37#, 39#, and 49#), and mycotoxin-protein conjugates were then coupled with biotin. Using streptavidin-phycoerythrin as a signal reporter protein, this direct competition multiple suspension array immunoassay was optimized. The results showed that the detection limits for zearalenone, fumonisin B1, deoxynivalenol, and aflatoxin B1 were 0.51, 6.0, 4.3, and 0.56 ng/mL, respectively, with detection ranges of 0.73-6.8, 11.6-110.3, 8.6-108.1, and 1.1-14.1 ng/mL, respectively. For the detection of the spiked samples, the recovery rates were between 92.3% and 115.5%. This method also shows a good correlation coefficient (r = 0.99, P < 0.01) with liquid chromatography- tandem mass spectrometry in the detection of toxins in commercial cereal and feedstuff samples. This suspension array immunoassay was high-throughput and accurate for the rapid quantitative detection of multiple mycotoxins in commercial cereal and feedstuff samples. © 2013 American Chemical Society.


Wang Y.-K.,Shanghai Key Laboratory of Veterinary Biotechnology | Yan Y.-X.,Shanghai Key Laboratory of Veterinary Biotechnology | Ji W.-H.,Shanghai Key Laboratory of Veterinary Biotechnology | Wang H.-A.,Shanghai Key Laboratory of Veterinary Biotechnology | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

A novel highly sensitive chemiluminescence immunoassay (CLIA) was developed to detect zearalenone in food samples by using both biotinylated zearalenone conjugates and gold (Au) nanoparticles labeled with streptavidin-horseradish peroxidase for signal amplification. Biotinylated zearalenone-ovalbumin conjugates and Au nanoparticles labeled with streptavidin-horseradish peroxidase were synthesized separately. The concentrations of immunoreagents and the reaction times of these immunoreagents were optimized to improve the performances of analytical methods. For the CLIA based on biotinylated zearalenone conjugates and Au nanoparticles labeled with streptavidin- horseradish peroxidase, the limit of detection was 0.008 ng/mL and the IC 50 was 0.11 ng/mL. The linear working range was 0.02-0.51 ng/mL. The cross-reactivities with the zearalenone analogues (α-zearalanol, zearalanone, α-zearalenol, β-zearalanol, and β-zearalenol) were 32, 17, 12, 0.3, and 0.1%, respectively. The recovery rates in spiked food samples were 97-117%, and the intraday and interday relative standard deviations were both <10%. Parallel analysis of natural food samples showed a good correlation between this novel CLIA and liquid chromatography-tandem mass spectrometry. This method provides a rapid, accurate, and highly sensitive method to determine levels of zearalenone in food samples. © 2013 American Chemical Society.


Cheng Y.,Shanghai Key Laboratory of Veterinary Biotechnology | Sun Y.,Chinese Academy of Agricultural Sciences | Wang H.,Shanghai Key Laboratory of Veterinary Biotechnology | Yan Y.,Shanghai Key Laboratory of Veterinary Biotechnology | And 2 more authors.
Journal of Immunology | Year: 2015

Stimulator of IFN genes (STING) is an adaptor that functions downstream of retinoic acid-inducible gene I (RIG-I) in mammalian cells; however, RIG-I is absent in chickens. We identified chicken STING (chSTING) as a critical mediator of virus-triggered type I IFN signaling in RIG-I-null chicken cells. Overexpression of chSTING in DF-1 cells inhibited Newcastle disease virus and avian influenza virus (AIV) viral replication and activated IRF-7 and NF-κB to induce expression of type I IFNs. Knockdown of endogenous chSTING abolished virus-triggered activation of IRF-7 and IFN-β and increased viral yield. chSTING was a critical component in the virus-triggered IRF-7 activation pathway and the cellular antiviral response. chSTING predominantly localized to the outer membrane of the endoplasmic reticulum and was also found in the mitochondrial membrane. Furthermore, knockdown of chSTING blocked polyinosinic-polycytidylic acid-, poly(deoxyadenylic-deoxythymidylic) acid-, and melanoma differentiation-associated gene 5 (MDA5)-stimulated induction of IFN-β. Coimmunoprecipitation experiments indicated that chicken MDA5 could interact with chSTING, and this interaction was enhanced by ectopically expressed chicken mitochondrial antiviral-signaling protein. Together, these results indicated that chSTING is an important regulator of chicken innate immune signaling and might be involved in the MDA5 signaling pathway in chicken cells. These results help with understanding the biological role of STING in innate immunity during evolution. Copyright © 2015 by The American Association of Immunologists, Inc.


Wang Y.-K.,Shanghai Key Laboratory of Veterinary Biotechnology | Yan Y.-X.,Shanghai Key Laboratory of Veterinary Biotechnology | Ji W.-H.,Shanghai Key Laboratory of Veterinary Biotechnology | Wang H.-A.,Shanghai Key Laboratory of Veterinary Biotechnology | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

A lateral flow dual immunoassay (LFDIA) was developed for rapid quantitative detection of zearalenone (ZEN) and fumonisin B1 (FB1) in corn and wheat samples on a single test strip. Two test lines and the control line on the nitrocellulose membrane were coated with ZEN and FB1 conjugates and goat anti-mouse IgG, respectively. Colloidal gold nanoparticles were conjugated with monoclonal antibodies against ZEN or FB1. The intensity of the test lines was analyzed by a photometric strip reader to determine the concentrations of ZEN and FB1 based on the calibration curves of known concentrations versus intensity readings. Test parameters such as types of buffers, ratio of the two gold-labeled antibodies, and dilution of the sample extracts and the gold-labeled antibodies were optimized. The detection limit was 0.35 and 5.23 ng/mL for ZEN and FB1, respectively, and the corresponding detection ranges were 0.94-7.52 and 9.34-100.45 ng/mL, respectively. Spiked and natural samples were analyzed using both LFDIA and liquid chromatography-tandem mass spectrometry. The two methods had a good correlation (R2 = 0.96). The dual quantitative LFDIA is sensitive, rapid, and easy-to-use for on-site testing of a large number of samples. © 2013 American Chemical Society.


Wang C.,Shanghai Key Laboratory of Veterinary Biotechnology | Lan D.,Shanghai Key Laboratory of Veterinary Biotechnology | Hua X.,Shanghai Key Laboratory of Veterinary Biotechnology
Virus Genes | Year: 2011

In this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of Shanghai in China during 2010. Forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (RT-PCR). The prevalence rate of porcine kobuvirus in the three pig farms which are located in Minhang District, Qingpu District and Fengxian District was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. We demonstrated that porcine kobuvirus infections are existent in certain domestic pigs in Shanghai and high prevalence of this virus was found in the piglets under the age of 6 weeks and in pigs with diarrhea in Shanghai. Twenty-seven representative strains of porcine kobuvirus detected in this study were randomly selected based on the equal distribution of the sampling date throughout the study and analyzed for their phylogenetic relationships with other kobuvirus reference strains. The phylogenetic analysis suggested the presence of multiple porcine kobuvirus lineages in Shanghai, China. © Springer Science+Business Media, LLC 2011.


Wang Y.-K.,Shanghai Key Laboratory of Veterinary Biotechnology | Wang Y.-C.,Shanghai Key Laboratory of Veterinary Biotechnology | Wang H.-A.,Shanghai Key Laboratory of Veterinary Biotechnology | Ji W.-H.,Shanghai Key Laboratory of Veterinary Biotechnology | And 2 more authors.
Food Control | Year: 2014

A sensitive enzyme-linked immunosorbent assay (ELISA) based on immunomagnetic beads (IMB-ELISA) was established using a magnetic-bead signal-enrichment system. The immunomagnetic beads were coated with polyclonal antibody directed against keyhole limpet hemocyanin (KLH), which were then coupled with a KLH-fumonisin B1 (FB1) conjugate. Anti-FB1 monoclonal antibody and sample extract were mixed and added to the immunomagnetic-bead solution. After the addition of horseradish peroxidase (HRP)-labeled goat anti-mouse antibody and the substrate solution, stop solution was added and the optical density of the reaction mixture was determined. To improve the performance of this method, the dilution of the immunomagnetic beads, the concentrations of the monoclonal antibody and HRP-labeled goat anti-mouse antibody, and the incubation time for the competition reaction were optimized. Based on the optimum conditions, the regression equation for this IMB-ELISA in quantifying FB1 was y=-0.3538x+0.703 (R2=0.9988). The detection limit and IC50 were 0.24ng/mL and 3.17ng/mL, respectively. The working range was 0.54-26.3ng/mL. The recovery rates were 80.4-114.7%, when the spiked concentrations ranged from 19.5 to 156.3μg/kg. This IMB-ELISA is accurate and more sensitive and less time-consuming than the conventional ELISA. © 2013 Elsevier Ltd.


He H.,Shanghai Key Laboratory of Veterinary Biotechnology
Bioengineered | Year: 2013

S100 proteins belong to a family of small, acidic, EF-hand Ca ( 2+) -binding proteins and have been found to exert both intracellular and extracellular functions in regulation of Ca ( 2+) homeostasis, cytoskeletal dynamics, cell cycle, motility and differentiation. As a result, they have been widely investigated for their association with diseases, such as, neurological diseases, cardiomyopathy, neoplasias and inflammatory diseases. To facilitate further studies of S100 proteins, we reported a simple and efficient method for the expression and purification of human S100A4 and S100A11 proteins in Escherichia coli. Since S100 proteins share many common physical and chemical characteristics, we expect that this approach can be extended to the production of most S100 proteins.

Loading Shanghai Key Laboratory of Veterinary Biotechnology collaborators
Loading Shanghai Key Laboratory of Veterinary Biotechnology collaborators