Zhao Z.,Shanghai University of Traditional Chinese Medicine |
Yu H.,Shanghai University of Traditional Chinese Medicine |
Peng Y.,Shanghai University of Traditional Chinese Medicine |
Ren H.,Shanghai University of Traditional Chinese Medicine |
And 9 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012
Objective: To observe the effect of traditional Chinese medicine formulas clearing away heat and promoting blood circulation-Biejiayinzi(BJYZ), Gexiazhuyu Tang(GXZYT) and Fugan Wan(FGW) on liver fibrosis in CCl 4 mice by screening and analyzing formula-syndrome database of kidney and liver fibrosis based on the principle of formula-syndrome, compared with pivot-harmonizing decoction. Method: Ten-week-old male C57BL/6 mice, with the weight of (20 ± 3) g, were randomly divided into 6 groups: the normal group, the model group, the BJYZ group, the GXZY group, the FGW group and the XST group. Except the normal group, other groups were abdominally injected with 10% CCl 4 olive oil solution a dose of 2 mL·kg -1 body weight for four weeks, three times each week. Meanwhile, the latter four groups were administered with extracts of BJYZ, GXZYT, FGW and XST, respectively, once every day, concomitantly continued CCl 4 administration. The normal and the model groups were given the same volume of deionized water. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT), Alb and TBil were detected by chemiluminescence. The hydroxyproline(HYP) content was detected by acid hydrolysis method. The hepatic collagen deposition was evaluated with Sirius red staining. Result: Compared with the normal group, the model group recorded notable decrease in weight and increase in the ratio of liver weight and body weight and the ratio of spleen weight and body weight, with obvious fatty degeneration and inflammatory necrosis in liver cells. Collagen fiber deposition was so notable to form fibrous septums and pseudolobules. The levels of serum ALT, AST, TBil, γ-GT, the HYP content in liver tissue and the deposition of hepatic collagen were significantly increased in the model group. Compared with model group, Serum AST were significantly decreased in BJYZ group as γ-GT decreased in the GXZYT group, without notable decrease in degeneration and inflammatory necrosis in liver cells and collagen deposition. The GXZYT group showed significant decrease in γ-GT, with slight improvement in degeneration and inflammatory necrosis in liver cells and reduction in collagen deposition. The ratio of liver weight and body weight, AST, γ-GT and HYP content were significantly decreased in the FGW group, with slight improvement in degeneration and inflammatory necrosis in liver cells and reduction in collagen deposition. The XST group showed decrease in the ratio of liver weight and body weight, with no obvious change in inflammation and fibrosis of hepatic tissue. Conclusion: FGW shows the best effect of prevention and treatment of liver fibrosis in CCl 4 mice.