Shanghai Key Laboratory of Protected Horticultural Technology

Shanghai, China

Shanghai Key Laboratory of Protected Horticultural Technology

Shanghai, China

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He K.-S.,Shanghai JiaoTong University | Chen D.-Y.,Shanghai JiaoTong University | Sun L.-J.,Chinese Academy of Agricultural Sciences | Liu Z.-L.,Shanghai Key Laboratory of Protected Horticultural Technology | Huang Z.-Y.,Shanghai JiaoTong University
Engineering Applications of Computational Fluid Mechanics | Year: 2015

In research literature, little attention has been applied to the effect of vent openings on greenhouse climates during cooling and dehumidification processes with natural ventilation, which would provide guidelines for greenhouse management. To address this problem, a 3D CFD model was successfully designed based on 11-span plastic greenhouses. The model was validated with the measured air temperature and relative humidity, and then used to investigate the effects of vent configuration and opening size on the greenhouse climate. The simulations show that the vent configuration affects greenhouse microclimate patterns and the spatial and temporal variations of the internal climate. Meanwhile, the vent opening size affects greenhouse dehumidification time, air temperature and relative humidity during the dehumidification process. Finally, the assessments of ventilation performance highlight that the roof plus side opening is most suitable for summer cooling, while the roof opening is most suitable for winter dehumidification. © 2015 The Author(s). Published by Taylor & Francis.


Deng M.,Chinese Academy of Sciences | Li Q.,Shanghai Institute of Technology | Li Q.,Shanghai Key Laboratory of Protected Horticultural Technology | Yang S.,Chinese Academy of Sciences | And 2 more authors.
Plant Systematics and Evolution | Year: 2013

Leaf epidermal features are considered to be taxonomically important in Fagaceae. In this study, we examined and compared leaf epidermal features of 112 specimens, representing 105 species and one variety of Lithocarpus from China and adjacent areas and Notholithocarpus densiflorus. As a result of the different interpretations of terms in previous studies, trichome terminology in Lithocarpus and its relatives was re-assessed aiming to reveal the trichome evolutionary patterns in Fagaceae. Twelve types of trichomes and five types of trichome bases were detected in Lithocarpus, among which the broad-based trichome (BBT) is newly reported. Stomata in Lithocarpus are restricted to the cyclocytic type and their size range is 28. 6 ± 8. 2 μm × 26. 5 ± 9. 3 μm. The distribution of epidermal features in Lithocarpus revealed three distinct morphological groups: glabrous, BBT, and appressed parallel tufts (APT). The importance of epidermal features across Fagaceae for taxon delimitation is evaluated. Species of Lithocarpus can be accurately identified by the presence of APT or flat epidermal cells combined with non-dark stained subsidiary cells and non-cutinized trichome bases only, or in addition, fasciculate trichome bases. The phylogenetic distribution of epidermal features and their evolutionary trends in Fagaceae is also discussed. © 2013 Springer-Verlag Wien.


Deng M.,Shanghai Institute of Technology | Deng M.,Shanghai Key Laboratory of Protected Horticultural Technology | Coombes A.,National Autonomous University of Mexico | Li Q.-S.,Shanghai Institute of Technology | Li Q.-S.,Shanghai Key Laboratory of Protected Horticultural Technology
Nordic Journal of Botany | Year: 2011

The name Quercus arbutifolia Hickel & A. Camus is lectotypifed. In addition, leaf epidermal features of Q. obovatifolia C. C. Huang, Q. arbutifolia and four other closely related species, Q. daimingshanensis (S. K. Lee) C. C. Huang, Q. merrillii Seemen, Q. litseoides Dunn and Q. tiaoloshanica Chun & W. C. Ko, were studied and compared using herbarium collections. Based on a comparison of leaf epidermal and other key taxonomic features, we conclude that Q. obovatifolia and Q. arbutifolia are identical. We thus reduce Q. obovatifolia to a new synonym of Q. arbutifolia. A key to the studied species is included. © 2011 The Authors.


Zhang Y.P.,Shanghai Academy of Agricultural science | Zhang Y.P.,Shanghai Key Laboratory of Protected Horticultural Technology | Zhu X.H.,Yangzhou University | Ding H.D.,Shanghai Key Laboratory of Protected Horticultural Technology | And 4 more authors.
Photosynthetica | Year: 2013

Brassinosteroids (BRs), an important class of plant steroidal hormones, play a significant role in the amelioration of various biotic and abiotic stresses. 24-epibrassinolide (EBR), an active brassinosteroid, was applied exogenously in different concentrations to characterize a role of BRs in tolerance of melon (Cucumis melo L.) to high temperature (HT) stress and to investigate photosynthetic performance of HT-stressed, Honglvzaocui (HT-tolerant) and Baiyuxiang (HTsensitive), melon variety. Under HT, Honglvzaocui showed higher biomass accumulation and a lower index of heat injury compared with the Baiyuxiang. The exogenous application of 1.0 mg L-1 EBR, the most effective concentration, alleviated dramatically the growth suppression caused by HT in both ecotypes. Similarly, EBR pretreatment of HTstressed plants attenuated the decrease in relative chlorophyll content, net photosynthetic rate, stomatal conductance, stomatal limitation, and water-use efficiency (WUE), as well as the maximal quantum yield of PSII photochemistry (Fv/Fm), the efficiency of excitation capture of open PSII center, the effective quantum yield of PSII photochemistry (ΦPSII), photochemical quenching coefficient, and the photon activity distribution coefficients of PSI (α). EBR pretreatment further inhibited the increase in intracellular CO2 concentration, leaf transpiration rate, minimal fluorescence of dark-adapted state, nonphotochemical quenching, thermal dissipation, and photon activity distribution coefficients of PSII. Results obtained here demonstrated that EBR could alleviate the detrimental effects of HT on the plant growth by improving photosynthesis in leaves, mainly reflected as up-regulation of photosynthetic pigment contents and photochemical activity associated with PSI. © 2013 Springer Science+Business Media Dordrecht.


Wu X.X.,Shanghai Academy of Agricultural science | Wu X.X.,Shanghai Key Laboratory of Protected Horticultural Technology | He J.,Yangzhou University | Zhu Z.W.,Shanghai Key Laboratory of Protected Horticultural Technology | And 3 more authors.
Biologia Plantarum | Year: 2014

This study was carried out to understand the mechanism of protection of plants under cold stress by exogenous 24-epibrassinolide (EBR). The eggplant (Solanum melongena L.) seedlings were pretreated with five concentrations of EBR (0, 0.05, 0.1, 0.2 and 0.4 °M) and then exposed to day/night temperatures of 10/5 °C for 8 d. The results show that EBR, especially 0.1 °M EBR, dramatically alleviated growth suppression and a decrease in chlorophyll content and photosynthetic rate caused by the cold stress. In addition, EBR also decreased malondialdehyde content and O2 ·- production rate induced by the cold stress, and increased the activities of superoxide dismutase, guaiacol peroxidase, catalase, and ascorbate peroxidase, and proline content. The results of the present study suggest that exogenous EBR could improve cold tolerance of eggplant by regulating photosynthesis and antioxidative systems. © 2014 Springer Science+Business Media Dordrecht.


Zhang Y.P.,Shanghai Academy of Agricultural science | Zhang Y.P.,Shanghai Key Laboratory of Protected Horticultural Technology | He J.,Yangzhou University | Yang S.J.,Shanghai Key Laboratory of Protected Horticultural Technology | And 2 more authors.
Biologia Plantarum | Year: 2014

This study was carried out to better understand the role of 24-epibrassinolide (EBR) in thermotolerance of melon (Cucumis melo L.). The melon seedlings were pretreated with various concentrations of EBR (0, 0.05, 0.1, 0.5, 1.0, and 1.5 mg dm-3) as foliar spray and then exposed to a high temperature (HT) stress. Exogenous EBR (0.5-1.5 mg dm-3) alleviated HT-caused growth suppression. In parallel, 1.0 mg dm-3 EBR attenuated the decrease in chlorophyll content, net photosynthetic rate, stomatal conductance, maximum quantum efficiency of photosystem (PS) II, quantum yield of PS II, and photochemical quenching of chlorophyll a fluorescence in HT-stressed plants, and inhibited transpiration rate and non-photochemical quenching. Furthermore, exogenous EBR also significantly reduced the content of malondialdehyde (MDA) and increased the content of soluble proteins and free proline, and activities of antioxidant enzymes including superoxide dismutase, guaiacol peroxidase, catalase, and ascorbate peroxidase under the HT stress. The results show that protective effects of EBR against the HT stress in the melon seedlings were most likely mediated through the improvement of photosynthesis and the stimulation of antioxidant capacity. © 2014 Springer Science+Business Media Dordrecht.


Ding H.-D.,Yangzhou University | Ding H.-D.,Shanghai Academy of Agricultural science | Zhu X.-H.,Yangzhou University | Zhu Z.-W.,Shanghai Academy of Agricultural science | And 4 more authors.
Biologia Plantarum | Year: 2012

The effects of exogenous 24-epibrassinolide (EBR) on the growth, oxidative damage, antioxidant system and ion contents in eggplant (Solanum melongena L.) seedlings under salt stress were investigated. Eggplant seedlings were exposed to 90 mM NaCl with 0, 0.025, 0.05, 0.10 and 0.20 mg dm -3 EBR for 10 d. EBR, especially at concentration 0.05 mg dm -3, alleviated growth suppression caused by NaCl stress, decreased electrolyte leakage, superoxide production and content of malondialdehyde and H 2O 2 in NaCl-treated plants. EBR also increased activities of superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase and the contents of ascorbic acid and reduced glutathione. Furthermore, we also found that Na +, Cl - contents were decreased, K +, Ca 2+ contents and K +/Na +, Ca 2+/Na + ratios were increased in the presence of EBR under salt stress. © 2012 Springer Science+Business Media B.V.


Wu X.-X.,Shanghai Academy of Agricultural science | Ding H.-D.,Shanghai Academy of Agricultural science | Chen J.-L.,Shanghai Key Laboratory of Protected Horticultural Technology | Zhang H.-J.,Yangzhou University | And 2 more authors.
African Journal of Biotechnology | Year: 2010

Exogenous sodium nitroprusside (SNP), a NO donor, was applied in this study to investigate the potential role of NO in photosynthetic performance of tomato (Lycopersicon esculentum L. cv. Hufan2560) seedlings under salt-stressed conditions. Exogenous NO alleviated the decrease in dry mass of shoot and root caused by salt stress. In parallel, NO application in salt-stressed plants attenuated the decrease in the photosynthetic parameters such as leaf chlorophyll, net photosynthetic rate (PN), stomatal conductance (gs), transpiration rate (E), the ratio of variable to maximum fluorescence (Fv/Fm), electron transport rate (ETR), the efficiency of excitation energy capture by open photosystem II (PSII) reaction centers (Fv′/Fm′), and the photochemical quenching coefficient (qP), and counteracted the increase in on-photochemical quenching coefficient (qN). Furthermore, the changes as mentioned above reversed by NO treatment are specific to salt stress since application of NO alone to tomato seedlings without salt stress had slight effects on the tested parameters. The results obtained here demonstrated that the photosynthetic performance was improved by NO application in salt-stressed plants and such an improvement was associated with an enhancement of gas-exchange and the actual PSII efficiency, which revealed an important role of NO in enhancing resistance of plants to salt stress. © 2010 Academic Journals.


Wu X.,Shanghai Academy of Agricultural science | Zhu W.,Shanghai Academy of Agricultural science | Zhu W.,Shanghai Key Laboratory of Protected Horticultural Technology | Zhang H.,Shanghai Academy of Agricultural science | And 2 more authors.
Acta Physiologiae Plantarum | Year: 2011

Nitric oxide (NO) has emerged as a key molecule involved in many physiological events in plants. To characterize roles of NO in tolerance of tomato (Lycopersicom esculentum Mill.) to salt stress, the protective effects of NO against salt-induced oxidative stress in the leaves of tomato cultivar Hufan1480 (salt-tolerant) and Hufan2496 (salt-sensitive) were evaluated. Under salt stress, Hufan1480 showed higher biomass accumulation, and less oxidative damage when compared with the Hufan2496. Application of exogenous sodium nitroprusside, a NO donor, dramatically alleviated growth suppression induced by salt stress in two tomato ecotypes, reflected by decreased malondialdehyde and O2 ·- production. Furthermore, the antioxidant enzymes superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase, the antioxidant metabolites ascorbate and reduced glutathione, and the osmosis molecules proline and soluble sugar were increased in both ecotypes in the presence of NO under salt stress. Therefore, the protective effect of NO against salt-induced oxidative damages in tomato seedlings is most likely mediated through stimulation of antioxidant system. © 2010 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.


He K.,Laboratory of Complex Dynamics Simulation | Chen D.,Laboratory of Complex Dynamics Simulation | Sun L.,Chinese Academy of Agricultural Sciences | Huang Z.,Laboratory of Complex Dynamics Simulation | Liu Z.,Shanghai Key Laboratory of Protected Horticultural Technology
International Journal of Ventilation | Year: 2015

The effects of vent configuration and span number on the microclimate in multi-span greenhouses were investigated. A three-dimensional (3-D) computational fluid dynamics (CFD) model was constructed based on an 11-span plastic greenhouse cultivated with 0.2 m-high lettuces. The model was verified with the temperature profile measured in the greenhouse. Then, it was used to explore the effects of vent configuration and span number on greenhouse climate. Simulations show that different vent configurations result in very different microclimate fields. Roof plus side opening has the best cooling and homogeneity performance and is thus recommended. The side openings, the first two windward roof openings and the last two roof openings play a central role during the ventilation process. A minor modification of the ventilation system induces a temperature decrease of 0.1 K and a humidity elevation of 0.3% and increases greenhouse climate homogeneity. These simulations also show that the ventilation rate exponentially decays with an increase of span number once side ventilators are opened, whereas it is maintained at 0.028 m3m-2s-1 with roof ventilation. Greenhouse climatic parameters and standard deviations are less influenced by span number than vent configuration. Finally, an analysis of inside climatic distribution reveals that strong temperature gradients occur from the windward to leeward area and above the ground. Differences of inside air velocity and temperature with and without the crop vary from 0 to 0.6 ms-1 and 0 to 1.2 K along the horizontal direction at 0.1 m above the ground, respectively. Photosynthetically active radiation (PAR) is symmetrically distributed in the greenhouse.

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