Shanghai, China
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Lai J.,Jiangnan University | Xin C.,Hebei United University | Zhao Y.,Shanghai Jahwa United Co. | Feng B.,Jiangnan University | And 5 more authors.
Molecules | Year: 2012

Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H 2O 2 were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC) test, chick chorioallantoic membrane (CAM) assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch tests. The optimum extract was obtained from bean sprouts grown to 0.5 cm. Extracts of black bean sprouts are safe and can be used as additives in anti-aging and whitening cosmetic products. © 2012 by the authors; licensee MDPI, Basel, Switzerland.


Lai J.,Jiangnan University | Xin C.,Hebei United University | Zhao Y.,Shanghai Jahwa United Co. | Feng B.,Jiangnan University | And 5 more authors.
Molecules | Year: 2013

Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of antioxidants from black soybean (Glycine max var) sprouts. Three influencing factors: liquid-solid ratio, period of ultrasonic assisted extraction and extraction temperature were investigated in the ultrasonic aqueous extraction. Then Response Surface Methodology (RSM) was applied to optimize the extraction process focused on DPPH radical-scavenging capacity of the antioxidants with respect to the above influencing factors. The best combination of each significant factor was determined by RSM design and optimum pretreatment conditions for maximum radical-scavenging capacity were established to be liquid-solid ratio of 29.19:1, extraction time of 32.13 min, and extraction temperature of 30 °C. Under these conditions, 67.60% of DPPH radical-scavenging capacity was observed experimentally, similar to the theoretical prediction of 66.36%. © 2013 by the authors.


Feng B.,Jiangnan University | Feng B.,Shanghai Jahwa United Co. | Ye W.-L.,Fudan University | Ma L.-J.,Shanghai Jahwa United Co. | And 4 more authors.
Life Sciences | Year: 2012

Aims: Recent studies have shown that dermal fibroblasts possess multiple types of voltage-dependent K + channels, and the activation of these channels induces apoptosis. In the present study, we aimed to investigate whether hydrogen peroxide (H 2O 2), an oxidative stress inducer, could modulate these channels or induce human dermal fibroblasts injury. Main methods: The effects of H 2O 2 on K + currents were studied using a whole-cell recording. Intracellular PKC levels were measured with a direct human PKC enzyme immunoassay kit. Cell viability was assessed using PI staining and apoptotic nuclei were detected with TdT-mediated digoxigenin-dUTP nick-end labelling assay (TUNEL) assay. Key findings: Treatment of cells with 100 μM H 2O 2 resulted in a partially reversible increase in non-inactivating outward K + currents and an alteration in the steady-state activation property of the channels. The H 2O 2-induced increase in K + currents was mimicked by a PKC activator, and was blocked by the PKC inhibitor or the large conductance Ca 2+-activited K + (BK) channel blockers. The intracellular PKC levels were significantly enhanced by H 2O 2 treatment in a concentration-dependent manner. After exposure to H 2O 2, evaluation of fibroblasts survival rate and damaged cell number with TUNEL-positive nuclei revealed an increased cell injury. Blocking the K + channels with blockers significantly decreased the H 2O 2-induced human dermal fibroblasts injury. Significance: Our results revealed that H 2O 2 could enhance BK currents by PKC pathway. Increased K + currents might be related to H 2O 2-induced human dermal fibroblasts injury. The results reported here contribute to our understanding of the mechanism underlying H 2O 2-induced human dermal fibroblasts injury. © 2012 Elsevier Inc. All rights reserved.


Feng B.,Jiangnan University | Feng B.,Shanghai Jahwa United Co. | Ma L.-J.,Shanghai Jahwa United Co. | Yao J.-J.,Fudan University | And 4 more authors.
Journal of Zhejiang University: Science B | Year: 2013

Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investigation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H2O 2). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1 301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-rich extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H 2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O 2, but application oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury. © 2013 Zhejiang University and Springer-Verlag.


Trademark
Shanghai Jahwa United Co. | Date: 2010-06-15

Cakes of toilet soap; bath lotion; cleaning preparations; detergent for household use; polishing wax; grinding preparations, namely, scouring preparations, abrasive preparations; ethereal oils; cream for whitening the skin; beauty masks; toilet water; cosmetics; dentifrices; incense; shampoos for pets.


Patent
Shanghai Jahwa United Co. | Date: 2012-03-21

The present invention relates to a resveratrol flexible liposome, comprising the following components with respect to the weight of the liposome: between 0.1 and 10% of resveratrol, between 0.5 and 10% of phospholipid and between 0.1 and 30% of softener. In addition, the present invention also relates to methods for preparing the resveratrol flexible liposome. The resveratrol flexible liposome of the present invention is highly flexible and has high penetrability, thus resulting in greatly increased transdermal absorption of the active ingredient.


Trademark
Shanghai Jahwa United Co. | Date: 2013-08-07

Shampoos for pets; antiperspirants (toiletries); toilet water; cosmetics; furbishing preparations; cleaning preparations; talcum powder; facial washes; incense; shampoo; essential oils; cakes of soap; dentifrices; bath lotion; shining preparations (polish).


Trademark
Shanghai Jahwa United Co. | Date: 2011-06-07

CAKES OF TOILET SOAP; COSMETICS AND MAKE-UP; COSMETICS IN FORM OF MILKS, LOTIONS AND EMULSIONS; DEODORANTS FOR PERSONAL USE; BATH LOTIONS; WASH FACE MILKS; SHAMPOOS; NAIL POLISH; CLEANING PREPARATIONS; ESSENTIAL OILS; BEAUTY MASKS; HAIR COLORANTS; COSMETICS FOR SKIN CARE; TOILET WATER; PERFUMES; ANTIPERSPIRANTS; SUNSCREEN PREPARATIONS; BREATH FRESHENING SPRAYS; POTPOURRIS; SHAMPOOS FOR PETS.


Trademark
Shanghai Jahwa United Co. | Date: 2016-08-22

Air fragrancing preparations; Beauty masks; Cakes of toilet soap; Cleansing creams; Cosmetics for animals; Cotton sticks for cosmetic purposes; Dish detergents; Essential oils; Facial cleansers; Incense; Make-up; Non-medicated mouth wash and rinse; Perfumes; Shampoos; Shining preparations; Washing preparations and laundry bleach.


Trademark
Shanghai Jahwa United Co. | Date: 2010-08-10

Cakes of toilet soap; bath lotion; wash face milk; essential oils; skin whitening creams; beauty masks; toilet water; cosmetics; sunscreen preparations; rouge; eye shadow; spot-removing creams; perfumes; fragrances potpourris; shampoos for pets.

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