Shanghai Institute of Health science

Shanghai, China

Shanghai Institute of Health science

Shanghai, China
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Wang Y.-S.,Shanghai JiaoTong University | Zhou J.,Shanghai Institute of Health science | Hong K.,Nanchang University | Cheng X.-S.,Nanchang University | Li Y.-G.,Shanghai JiaoTong University
Cellular Physiology and Biochemistry | Year: 2015

Background/Aims: MicroRNAs play regulatory role in cardiovascular disease. MicroRNA-223 (miR-223) was found to be expressed abundantly in myocardium. TNNI3K, a novel cardiac troponin I (cTnI)-interacting and cardiac hypertrophy related kinase, is computationally predicted as a potential target of miR-223. This study was designed to investigate the cellular and molecular effects of miR-223 on cardiomyoctye hypertrophy, focusing on the role of TNNI3K. Methods: Neonatal rat cardiomyocytes (CMs) were cultured, and CMs hypertrophy was induced by endothelin-1 (ET-1). In vivo cardiac hypertrophy was induced by transverse aorta constriction (TAC) in rats. Expression of miR-223 in CMs and myocardium was detected by real-time PCR (RT-PCR). MiR-223 and TNNI3K were overexpressed in CMs via chemically modifed sense RNA (miR-223 mimic) transfection or recombinant adenovirus infection, respectively. Cell size was measured by surface area calculation using fluorescence microscopy after anti-α-actinin staining. Expression of hypertrophy-related genes was detected by RT-PCR. The protein expression of TNNI3K and cTnI was determined by Western blots. Luciferase assay was employed to confirm the direct binding of miR-223 to the 3'UTR of TNNI3K mRNA. Intracellular calcium was measured by sensitive fluorescent indicator (Furo-2). Video-based edge detection system was employed to measure cardiomyocyte contractility. Results: MiR-223 was downregulated in ET-1 induced hypertrophic CMs and in hypertrophic myocardium compared with respective controls. MiR-223 overexpression in CMs alleviated ET-1 induced hypertrophy, evidenced by smaller cell surface area and downregulated ANP, α-actinin, Myh6 and Myh7 expression. Luciferase reporter gene assay showed that TNNI3K serves as a direct target gene of miR-223. In miR-223-overexpressed CMs, the protein expression of TNNI3K was significantly downregulated. MiR-223 overexpression also rescued the upregulated TNNI3K expression in hypertrophic CMs. Furthermore, cTnI phosphorylation was downregulated post miR-223 overexpression. Ad.rTNNI3K increased intracellular Ca2+ concentrations and cell shortening in CMs, while miR-223 overexpression significantly rescued these hypertrophic effects. Conclusion: By direct targeting TNNI3K, miR-223 could suppress CMs hypertrophy via downregulating cTnI phosphorylation, reducing intracellular Ca2+ and contractility of CMs. miR-223/TNNI3K axis may thus be major players of CMs hypertrophy. © 2015 S. Karger AG, Basel.


Tan P.-P.,Shanghai JiaoTong University | Yuan H.-H.,Shanghai Institute of Health science | Zhu X.,Shanghai JiaoTong University | Cui Y.-Y.,Shanghai JiaoTong University | And 5 more authors.
CNS Neuroscience and Therapeutics | Year: 2014

Aims: Muscarinic acetylcholine receptor agonist pilocarpine reduces intraocular pressure (IOP) of glaucoma mainly by stimulating ciliary muscle contraction and then increasing aqueous outflow. It is of our great interest to know whether pilocarpine has the additional properties of retinal neuroprotection independent of IOP lowering in vitro and in vivo models. Methods: In rat primary retinal cultures, cell viability was measured using an MTT assay and the trypan blue exclusion method, respectively. Retinal ganglion cells (RGCs) were identified by immunofluorescence and quantified by flow cytometry. For the in vivo study, the retinal damage after retinal ischemia/reperfusion injury in rats was evaluated by histopathological study using hematoxylin and eosin staining, transmission electron microscopy, and immunohistochemical study on cleaved caspase-3, caspase-3, and ChAT. Results: Pretreatment of pilocarpine attenuated glutamate-induced neurotoxicity of primary retinal neurons in a dose-dependent manner. Protection of pilocarpine in both retinal neurons and RGCs was largely abolished by the nonselective muscarinic receptor antagonist atropine and the M1-selective muscarinic receptor antagonist pirenzepine. After ischemia/reperfusion injury in retina, the inner retinal degeneration occurred including ganglion cell layer thinning and neuron lost, and the optic nerve underwent vacuolar changes. These degenerative changes were significantly lessened by topical application of 2% pilocarpine. In addition, the protective effect of pilocarpine on the ischemic rat retina was favorably reflected by downregulating the expression of activated apoptosis marker cleaved caspase-3 and caspase-3 and upregulating the expression of cholinergic cell marker ChAT. Conclusions: Taken together, this highlights pilocarpine through the activation of muscarinic receptors appear to afford significant protection against retinal neurons damage and optic nerve degeneration at clinically relevant concentrations. These data also further support muscarinic receptors as potential therapeutic neuroprotective targets in glaucoma. © 2013 John Wiley & Sons Ltd.


Sun Y.-G.,Shanghai JiaoTong University | Wang X.-Y.,Shanghai Institute of Health Science | Chen X.,Shanghai JiaoTong University | Shen C.-X.,Shanghai JiaoTong University | Li Y.-G.,Shanghai JiaoTong University
International Journal of Clinical and Experimental Pathology | Year: 2015

Hydrogen sulfide (H2S), produced by cystanthionine-γ-lysase (CSE) in the cardiovascular system, is an endogenous gaseous mediator exerting pronounced physiological effects as the third gasotransmitter in addition to nitric oxide (NO) and carbon monoxide (CO). Accumulating evidence indicated that H2S could mediate the cardioprotective effects in myocardial ischemia model. Ventricular arrhythmia is the most important risk factor for cardiac mortality and sudden death after acute myocardial infarction (AMI). The potential impact of H2S on cardiomyocytes electrical remodeling post ischemic insult is not fully explored now. Present study investigated the role of H2S on cardiomyocytes electrical remodeling in rats with ischemia/reperfusion injury. H2S concentration was reduced and arrhythmia score was increased in this model. CSE mRNA level was also upregulated in the ischemic myocardium. Exposure to exogenous NaHS reduced the action potential duration (APD), inhibited L-type Ca2+ channels and activated KATP channels in cardiomyocytes isolated from ischemic myocardium Exogenous H2S application improves electrical remodeling in cardiomyocytes isolated from ischemic myocardium. These results indicated that reduced H2S level might be linked to ischemia/reperfusion induced arrhythmias.


Xu Z.-M.,Shanghai Institute of Health science | Xu D.,Shanghai JiaoTong University
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To investigate effectiveness of vocation practicability physical education (VPPE) in order to strengthen physique and enhance vocation-related abilities of nursing students. Methods: A total of 228 female students from Shanghai Institute of Health Sciences were enrolled in this program in 2012, who were randomly divided into VPPE group (n=115) and control group (n=113). The changes of physical quality, body composition, and the cardio-pulmonary resuscitation (CPR) operating time were measured after 16-week-long practice. Results: Before the trial, there were no significant differences among the parameters of physical quality, body composition, and CPR operating time of both groups (P>0.05). After the trial, the parameters were all improved, but the changes of VPPE group were obviously higher than those of control group. Compared to control group, in physical quality, the abilities of long-distance race (800 m) and handgrip strength improved; the body fat decreased and muscles of extremities except of the left arm increased; and the CPR operating time increased in VPPE group (P<0.05, P<0.01). Conclusion: The VPPE is effective and practicable in improving the physical quality and ability of students in adapting the future work.


PubMed | Shanghai institute of Health Science and Shanghai JiaoTong University
Type: Journal Article | Journal: Molecular nutrition & food research | Year: 2016

The NLRP3 inflammasome responds to various pathogen-derived factors and danger-associated molecules, mediating IL-1 maturation, therefore is involved in multiple inflammatory diseases. Curcumin has been shown to possess strong anti-inflammatory activity, but the underlying mechanism is not fully understood. Here, we sought to investigate the role and mechanism of curcumin on the inhibition of mature IL-1 production via the regulation of NLRP3 inflammasome.Curcumin dramatically inhibited the production of mature IL-1 in LPS-primed macrophages triggered by multiple NLRP3 inflammasome activators, and also reduced the level of cleaved caspase-1 as measured by western blot and ELISA. Curcumin prevented K(+) efflux, the common trigger for NLRP3 inflammasome activation, and attenuated lysosomes disruption and intracellular ROS formation as well. The inhibition of NLRP3 inflammasome by curcumin was in part mediated via the suppression of extracellular regulated protein kinases phosphorylation. Furthermore, administration of curcumin significantly reduced peritoneal IL-1 and HMGB-1 concentration induced by LPS and improved the survival of mice suffering from lethal endotoxic shock.Curcumin potently inhibits the activation of NLRP3 inflammasome which may contribute to its anti-inflammatory activity. Our finding offers a mechanistic basis for the therapeutic potential of curcumin in septic shock and other NLRP3 inflammasome-driven diseases.


Zheng L.,Shenyang University | Li J.,Tongji University | Hu D.,Tongji University | Luo Y.,Shanghai Institute of Health science | And 4 more authors.
Journal of Atherosclerosis and Thrombosis | Year: 2010

Aims: Whether a low ankle-brachial index can improve the prediction of all-cause and cardiovascular mortality on top of conventional risk factors remains unclear among patients with ischemic heart disease. The present study aimed to assess the association between the ankle-brachial index and mortality in Chinese patients. Methods: This was an observational prospective study and 1,800 Chinese patients aged ≥ 35 years were followed-up from 2004 to 2007-2008. Results: There were 280 deaths, of which 165 were attributable to cardiovascular disease. Compared with patients with an ankle-brachial index ≥ 1.1, the risk of mortality increased linearly in lower ankle-brachial index categories: patients with an ankle-brachial index of 0.9 to 1.1, 0.7 to 0.9, < 0.7 had hazard ratios of 1.60, 2.07, and 3.08 for all-cause mortality and 1.89, 2.33, and 4.09 for cardiovascular mortality (p for trend < 0.001), respectively. Addition of the ankle-brachial index significantly (p<0.001) increased the predictive value of the model for 3-year deaths compared with a model including risk factors alone. Comparison of areas under receiver operator characteristics curves confirmed that a model including the ankle-brachial index discriminated better than without. Conclusion: There was an inverse association between the ankle-brachial index and mortality. Addition of the ankle-brachial index significantly improved the prediction of 3-year mortality over and above that of conventional risk factors. We recommend that the ankle-brachial index be incorporated into prognostic assessment for patients with ischemic heart disease.


Luo Y.,Shanghai Institute of Health science | Luo Y.,Tongji University | Li X.,Tongji University | Li J.,Tongji University | And 5 more authors.
Vascular Medicine | Year: 2010

The purpose of this study was to investigate the association between chronic kidney disease (CKD) and peripheral arterial disease (PAD) and examine the combined effect of CKD and PAD on all-cause and cardiovascular disease (CVD) mortality. The Chinese Ankle Brachial Index Cohort consisted of 3732 adults aged 35 years or older enrolled in 2004 and followed-up in 2007. Complete baseline data were compiled on 3610 people which were examined in the final analysis. Mortality surveillance was completed from December 2007 to February 2008. Survival analysis was used to compare the survival rate in different CKD/PAD groups. The relative risks (RR) of death from all-cause and CVD were compared using a Cox regression model. It was found that the prevalence of PAD in patients with and without CKD was 41.9% and 22.3%, respectively (p < 0.001). The survival rate for the CKD and PAD group was significantly lower than that for any single disease, for both all-cause and CVD mortality (log-rank: p < 0.001). In conclusion, CKD is a risk factor for PAD. The combined CKD and PAD patients had the highest risk for all-cause and CVD mortality. Early recognition of risk can be made by taking an ankleg-brachial index measurement of PAD; a corresponding laboratory assessment should be used as a measurement of renal function for PAD patients.


Li X.,Tongji University | Li X.,Shanghai Institute of Health science | Luo Y.,Shanghai Institute of Health science | Xu Y.,Tongji University | And 2 more authors.
Journal of Human Hypertension | Year: 2010

This study aimed to investigate the relationship of ankle-brachial index (ABI) with all-cause mortality and cardiovascular (CV) mortality in Chinese inpatients after 3 years of follow-up. All the participants were inpatients at high risk of atherosclerosis and enrolled from July to November 2004. A total of 3210 patients were followed up until an end point was reached or until February 2008. The mean follow-up time was 37.65±1.54 months. Patients with ABI ≤0.4 were significantly older than other ABI categories (P<0.001) at baseline. The all-cause and CV disease (CVD) mortality were highest (37.7 and 27.5%, respectively) after a 3-year follow-up in patients with ABI ≤0.4. There was a significant increasing tendency in mortality with decreasing ABI levels (P<0.001). The Kaplan-Meier curves of survival showed a decreasing survival rate with the ABI decreasing, not only for all-cause mortality but also for CVD mortality (P<0.001). Adjusted for other risk factors, patients with ABI ≤0.4 were 3.105 times (95% CI 1.936-4.979) as likely to die as those with ABI in the range of 1.00-1.4; patients with ABI ≤0.4 were about five times (95% CI 2.740-8.388) as likely to die of CVD as those with ABI in the range of 1.00-1.4. Even patients with ABI in the range of 0.41-0.90 were more than 1.5 times as likely to die (relative risk1.534, 95% CI 1.199-1.962), or die of CVD (relative risk2.031, 95% CI 1.479-2.789) as those with ABI in the range of 1.00-1.4. Low ABI is related to a higher all-cause and CV mortality compared with normal ABI. ABI as a marker of atherosclerosis should be routinely evaluated to assess the risk of CV mortality in Chinese patients. © 2010 Macmillan Publishers Limited.


Tang H.-M.,Shanghai Institute of Health science | Cen J.,Shanghai Institute of Health science
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2013

Objective: To summarize the research trends of nursing education in China in the past years, and analyse the reform and development trends of nursing education. Methods: Literatures published between January 2003 and December 2011 were retrieved with "education, nursing" as the subject terms in China Biology Medicine disc. The number of enrolled literatures, affiliations of authors, journal sources of literatures and themes of literatures were statistically analyzed. Results: A total of 28622 literatures were obtained. The number of enrolled literatures on nursing education had significantly increased since 2006, and reached 6900 in 2011. The affiliations of authors were mainly vocational schools, medical colleges and hospitals. The journal sources included nursing related journals, clinical medicine journals and medical education journals. The themes of literatures focused on nursing education objectives, curriculum setting, teaching methods, and education reform and assessment. Conclusion: The research trends of nursing education are associated with development of multi-level nursing training objectives, nursing database, localized courses, diversified teaching methods and standard evaluation system for nursing education.


Liu X.-R.,Shanghai Institute of Health science
Chinese Journal of New Drugs | Year: 2011

Objective: To study the formulation and preparation of lornoxicam orally disintegrating tablets, and to evaluate its quality. Methods: The formulation of orally disintegrating tablets was optimized with disintegrating time and taste as reference parameters by an orthogonal design. The tablets were prepared by a directly compressed method. The properties of the tablets, such as dissolution and disintegrating time in vitro, were determined. Results: The orally disintegrating tablets were integrity and smooth with desirable taste and feel in the mouth. The disintegrating time was less than 20 s, and the cumulative dissolution percentage was higher than 85% within 5 min. Conclusion: The lornoxicam orally disintegrating tablets developed in this work will hopefully contribute to improve drug administration to patient.

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