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Xu Z.-M.,Shanghai Institute of Health science | Xu D.,Shanghai JiaoTong University
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To investigate effectiveness of vocation practicability physical education (VPPE) in order to strengthen physique and enhance vocation-related abilities of nursing students. Methods: A total of 228 female students from Shanghai Institute of Health Sciences were enrolled in this program in 2012, who were randomly divided into VPPE group (n=115) and control group (n=113). The changes of physical quality, body composition, and the cardio-pulmonary resuscitation (CPR) operating time were measured after 16-week-long practice. Results: Before the trial, there were no significant differences among the parameters of physical quality, body composition, and CPR operating time of both groups (P>0.05). After the trial, the parameters were all improved, but the changes of VPPE group were obviously higher than those of control group. Compared to control group, in physical quality, the abilities of long-distance race (800 m) and handgrip strength improved; the body fat decreased and muscles of extremities except of the left arm increased; and the CPR operating time increased in VPPE group (P<0.05, P<0.01). Conclusion: The VPPE is effective and practicable in improving the physical quality and ability of students in adapting the future work.

Zheng L.,Shenyang University | Li J.,Tongji University | Hu D.,Tongji University | Luo Y.,Shanghai Institute of Health science | And 4 more authors.
Journal of Atherosclerosis and Thrombosis | Year: 2010

Aims: Whether a low ankle-brachial index can improve the prediction of all-cause and cardiovascular mortality on top of conventional risk factors remains unclear among patients with ischemic heart disease. The present study aimed to assess the association between the ankle-brachial index and mortality in Chinese patients. Methods: This was an observational prospective study and 1,800 Chinese patients aged ≥ 35 years were followed-up from 2004 to 2007-2008. Results: There were 280 deaths, of which 165 were attributable to cardiovascular disease. Compared with patients with an ankle-brachial index ≥ 1.1, the risk of mortality increased linearly in lower ankle-brachial index categories: patients with an ankle-brachial index of 0.9 to 1.1, 0.7 to 0.9, < 0.7 had hazard ratios of 1.60, 2.07, and 3.08 for all-cause mortality and 1.89, 2.33, and 4.09 for cardiovascular mortality (p for trend < 0.001), respectively. Addition of the ankle-brachial index significantly (p<0.001) increased the predictive value of the model for 3-year deaths compared with a model including risk factors alone. Comparison of areas under receiver operator characteristics curves confirmed that a model including the ankle-brachial index discriminated better than without. Conclusion: There was an inverse association between the ankle-brachial index and mortality. Addition of the ankle-brachial index significantly improved the prediction of 3-year mortality over and above that of conventional risk factors. We recommend that the ankle-brachial index be incorporated into prognostic assessment for patients with ischemic heart disease.

Sun Y.-G.,Shanghai JiaoTong University | Wang X.-Y.,Shanghai Institute of Health science | Chen X.,Shanghai JiaoTong University | Shen C.-X.,Shanghai JiaoTong University | Li Y.-G.,Shanghai JiaoTong University
International Journal of Clinical and Experimental Pathology | Year: 2015

Hydrogen sulfide (H2S), produced by cystanthionine-γ-lysase (CSE) in the cardiovascular system, is an endogenous gaseous mediator exerting pronounced physiological effects as the third gasotransmitter in addition to nitric oxide (NO) and carbon monoxide (CO). Accumulating evidence indicated that H2S could mediate the cardioprotective effects in myocardial ischemia model. Ventricular arrhythmia is the most important risk factor for cardiac mortality and sudden death after acute myocardial infarction (AMI). The potential impact of H2S on cardiomyocytes electrical remodeling post ischemic insult is not fully explored now. Present study investigated the role of H2S on cardiomyocytes electrical remodeling in rats with ischemia/reperfusion injury. H2S concentration was reduced and arrhythmia score was increased in this model. CSE mRNA level was also upregulated in the ischemic myocardium. Exposure to exogenous NaHS reduced the action potential duration (APD), inhibited L-type Ca2+ channels and activated KATP channels in cardiomyocytes isolated from ischemic myocardium Exogenous H2S application improves electrical remodeling in cardiomyocytes isolated from ischemic myocardium. These results indicated that reduced H2S level might be linked to ischemia/reperfusion induced arrhythmias.

Wang Y.-S.,Shanghai JiaoTong University | Zhou J.,Shanghai Institute of Health science | Hong K.,Nanchang University | Cheng X.-S.,Nanchang University | Li Y.-G.,Shanghai JiaoTong University
Cellular Physiology and Biochemistry | Year: 2015

Background/Aims: MicroRNAs play regulatory role in cardiovascular disease. MicroRNA-223 (miR-223) was found to be expressed abundantly in myocardium. TNNI3K, a novel cardiac troponin I (cTnI)-interacting and cardiac hypertrophy related kinase, is computationally predicted as a potential target of miR-223. This study was designed to investigate the cellular and molecular effects of miR-223 on cardiomyoctye hypertrophy, focusing on the role of TNNI3K. Methods: Neonatal rat cardiomyocytes (CMs) were cultured, and CMs hypertrophy was induced by endothelin-1 (ET-1). In vivo cardiac hypertrophy was induced by transverse aorta constriction (TAC) in rats. Expression of miR-223 in CMs and myocardium was detected by real-time PCR (RT-PCR). MiR-223 and TNNI3K were overexpressed in CMs via chemically modifed sense RNA (miR-223 mimic) transfection or recombinant adenovirus infection, respectively. Cell size was measured by surface area calculation using fluorescence microscopy after anti-α-actinin staining. Expression of hypertrophy-related genes was detected by RT-PCR. The protein expression of TNNI3K and cTnI was determined by Western blots. Luciferase assay was employed to confirm the direct binding of miR-223 to the 3'UTR of TNNI3K mRNA. Intracellular calcium was measured by sensitive fluorescent indicator (Furo-2). Video-based edge detection system was employed to measure cardiomyocyte contractility. Results: MiR-223 was downregulated in ET-1 induced hypertrophic CMs and in hypertrophic myocardium compared with respective controls. MiR-223 overexpression in CMs alleviated ET-1 induced hypertrophy, evidenced by smaller cell surface area and downregulated ANP, α-actinin, Myh6 and Myh7 expression. Luciferase reporter gene assay showed that TNNI3K serves as a direct target gene of miR-223. In miR-223-overexpressed CMs, the protein expression of TNNI3K was significantly downregulated. MiR-223 overexpression also rescued the upregulated TNNI3K expression in hypertrophic CMs. Furthermore, cTnI phosphorylation was downregulated post miR-223 overexpression. Ad.rTNNI3K increased intracellular Ca2+ concentrations and cell shortening in CMs, while miR-223 overexpression significantly rescued these hypertrophic effects. Conclusion: By direct targeting TNNI3K, miR-223 could suppress CMs hypertrophy via downregulating cTnI phosphorylation, reducing intracellular Ca2+ and contractility of CMs. miR-223/TNNI3K axis may thus be major players of CMs hypertrophy. © 2015 S. Karger AG, Basel.

Liu X.-R.,Shanghai Institute of Health science
Chinese Journal of New Drugs | Year: 2011

Objective: To study the formulation and preparation of lornoxicam orally disintegrating tablets, and to evaluate its quality. Methods: The formulation of orally disintegrating tablets was optimized with disintegrating time and taste as reference parameters by an orthogonal design. The tablets were prepared by a directly compressed method. The properties of the tablets, such as dissolution and disintegrating time in vitro, were determined. Results: The orally disintegrating tablets were integrity and smooth with desirable taste and feel in the mouth. The disintegrating time was less than 20 s, and the cumulative dissolution percentage was higher than 85% within 5 min. Conclusion: The lornoxicam orally disintegrating tablets developed in this work will hopefully contribute to improve drug administration to patient.

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