Jiang H.,Shanghai Institute of Endocrinology and Metabolism |
Ye X.-P.,Shanghai Institute of Endocrinology and Metabolism |
Yang Z.-Y.,Shanghai JiaoTong University |
Zhan M.,Shanghai Institute of Endocrinology and Metabolism |
And 6 more authors.
Journal of Molecular Endocrinology | Year: 2013
There is a high incidence of metabolic syndrome among patients with primary aldosteronism PA, which has recently been associated with an unfavorable cardiometabolic profile. However, the underlying mechanisms have not been clarified in detail. Characterizing aldosterone Ald target genes in adipocytes will help us to elucidate the deleterious effects associated with excess Ald. Apelin, a novel adipokine, exerts beneficial effects on obesityassociated disorders and cardiovascular homeostasis. The objective of this study was to investigate the effects of high Ald levels on apelin expression and secretion and the underlying mechanisms involved in adipocytes. In vivo, a single-dose Ald injection acutely decreased apelin serum levels and adipose tissue apelin production, which demonstrates a clear inverse relationship between the levels of plasma Ald and plasma apelin. Experiments using 3T3-L1 adipocytes showed that Ald decreased apelin expression and secretion in a time- and dose-dependent manner. This effect was reversed by glucocorticoid receptor GR antagonists or GR NR3C1 knockdown; furthermore, putative HREs were identified in the apelin promoter. Subsequently, we verified that both glucocorticoids and mineralocorticoids regulated apelin expression through GR activation, although no synergistic effect was observed. Additionally, detailed potential mechanisms involved a p38 MAPK signaling pathway. In conclusion, our findings strengthen the fact that there is a direct interaction between Ald and apelin in adipocytes, which has important implications for hyperaldosteronism or PA-associated cardiometabolic syndrome and hoists apelin on the list of potent therapeutic targets for PA. © 2013 Society for Endocrinology.
Zhao L.,Shanghai JiaoTong University |
Zhao L.,Shanghai Institute of Endocrinology and Metabolism |
Zhao L.,Endocrine And Metabolic sts Of Shanghai Universities Eisu |
Zhang M.-J.,Shanghai JiaoTong University |
And 24 more authors.
Clinical Biochemistry | Year: 2011
Objective: This study was to establish biochemical thresholds for the intravenous calcium suppression test in the early diagnosis of primary hyperparathyroidism (PHPT). Design and methods: One hundred and thirty-three patients were divided into three groups: Group 1: surgically proven hypercalcemic PHPT, Group 2 surgically proven mild PHPT, and Group 3: normocalcemia with elevated serum PTH levels. Intravenous calcium suppression tests were performed in Groups 2 and 3 as well as in 20 controls with normal serum calcium and PTH concentrations. Results: The serum PTH inhibition rate (PTH-IR) was less pronounced in Group 2 compared with Group 3 (P < 0.001) and the controls (P < 0.001). Receiver operating characteristic curve analysis suggests that a serum calcium level higher than 2.43. mmol/L and a PTH-IR less than 73% may differentiate Group 2 from normal controls. Conclusion: It is quite useful to combine serum calcium levels with the PTH-IR to identify patients at early stage of PHPT, even in the presence of vitamin D deficiency. © 2011 The Canadian Society of Clinical Chemists.
Zhao Z.-F.,Gongli Hospital |
Zhao Y.-J.,Shanghai JiaoTong University |
Gu M.-J.,Gongli Hospital |
Cui J.-L.,Shanghai Institute of Endocrinology and Metabolism |
Wang S.,Shanghai JiaoTong University
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2013
Objective: To investigate the effect of Ganoderma lucidum polysaccharides on hyperthyroidism and liver injury in the mice model of Graves' disease (GD). Methods: GD mice model was constructed by immunizing BALB/c mice with thyroid stimulating hormone receptor-A (TSHR-A). Serum thyroxine (T4), thyroid stimulating hormone receptor antibody (TRAb), and liver function parameters were measured, and correlation analysis was performed. The mice were intragastrically managed with pure water, low-dose Ganoderma lucidum polysaccharides (100 mg·kg-1·d-1) and high-dose Ganoderma lucidum polysaccharides (400 mg·kg-1·d-1) respectively, and the change of serum T4, TRAb, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined. Results: Serum ALT, AST and ALP in mice with hyperthyroidism in model group were significantly higher than those in mice without hyperthyroidism in model group and mice in control group (P<0.05). Serum AST and ALP were positively significantly related to serum T4 (r=0.585, P<0.05; r=0.744, P<0.05), but were not related to serum TRAb (P>0.05). Serum T4 and TRAb in model mice were not significantly changed after treatment with Ganoderma lucidum polysaccharides (P>0.05). Serum ALT and ALP in mice with hyperthyroidism in model group treated with low-dose Ganoderma lucidum polysaccharides were significantly superior to those in pure water group (P<0.05). Conclusion: Ganoderma lucidum polysaccharides may not work on hyperthyroidism improvement in GD mice model, but can improve the liver injury caused by hyperthyroidism.
Hong J.,Shanghai Institute of Endocrinology and Metabolism |
Shi J.,Shanghai Institute of Endocrinology and Metabolism |
Qi L.,Brigham and Women's Hospital |
Cui B.,Shanghai JiaoTong University |
And 12 more authors.
International Journal of Obesity | Year: 2013
Objective:Birth weight reflects prenatal metabolic adaption and has been related to later-life obesity risk. This study aimed to evaluate whether birth weight modifies the effect of genetic susceptibility on obesity risk in young Chinese.Methods:We recruited 540 young (14-30 years) and obese patients (body mass index, BMI≥30 kg m -2), and 500 age- and sex-matched normal-weight healthy individuals (BMI<23 kg m -2). We genotyped 23 BMI-associated genetic variants identified from recent genome-wide association studies (GWAS) in Caucasians with European ancestry with minor allele frequency>0.05 in HapMap Han Chinese in Beijing, China.Results:Six loci, including SEC16B, GNPDA2, BDNF, FTO, MC4R and TMEM160, were significantly associated with obesity risk, with odds ratio from 1.314 to 1.701. The 23 risk loci accounted for 6.38% of the genetic variance in obesity. We created two genetic risk scores (GRSs) by summing the risk alleles of all 23 (GRS1) and 6 obesity-associated (GRS2) genetic variants. Prediction of obesity was significantly improved (P<0.001) when the GRS1 and GRS2 were added to a model with age and gender, with improvement of discrimination for obesity by 0.8% and 2.7%, respectively. In addition, we found that the two GRSs interacted with birth weight in relation to obesity (P interaction <0.001). The genetic effect appeared to be more pronounced in individuals with normal range of birth weight (25-75%) than those with either low (<25%) or high (>75%) birth weight.Conclusion:We confirmed the associations of the single-nucleotide polymorphism tagging six loci reported in recent GWAS with obesity in young Chinese. Our data also suggest birth weight may significantly modify genetic susceptibility to obesity risk. © 2013 Macmillan Publishers Limited.