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Chen B.-Q.,Huaqiao University | Kankala R.K.,Huaqiao University | Chen A.-Z.,Huaqiao University | Yang D.-Z.,Huaqiao University | And 5 more authors.
International Journal of Nanomedicine | Year: 2017

Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(l-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering. © 2017 Chen et al.

Zhang C.-J.,Fudan University | Zhang C.-J.,Shanghai Institute of Cardiovascular Disease | Zhang C.-J.,Shanghai Institute of Medical Imaging | Cheng L.-L.,Fudan University | And 2 more authors.
Fudan University Journal of Medical Sciences | Year: 2017

MicroRNA (miRNA) is a class of 19-25-nucleotide non-coding RNAs that plays an important role in cellular proliferation, differentiation, development as well as cell death. The miRNA that can be detected in the plasma is known as circulating miRNA, and it can exist stable in the circulation of the blood. Recently, circulating miRNA has been increasingly suggested as novel potential biomarkers of a series of myocardial lesions. This review article summarized the progress of clinical and experimental studies of circulating miRNA as early diagnosis index for cardiopathologic conditions, including acute myocardial infarction, heart failure along with drug-induced cardiotoxicity. © 2017, Editorial Department of Fudan University Journal of Medical Sciences. All right reserved.

Song F.-Y.,Fudan University | Song F.-Y.,Shanghai Institute of Cardiovascular Disease | Song F.-Y.,Shanghai Institute of Medical Imaging | Cheng L.-L.,Fudan University | And 2 more authors.
Fudan University Journal of Medical Sciences | Year: 2016

Anthracycline provides considerable therapeutic benefit in most patients with lymphoma. However, the cardiotoxicity of anthracycline in these patients cannot be ignored. In recent years, with the right ventricular function being given attention gradually, subclinical dysfunction of right ventricle induced by anthracycline has attracted worldwide attention. This review was concentrated on various methods of detecting the subclinical cardiotoxicity of anthracycline on right ventricle in patients with lymphoma, such as biological markers, electrocardiogram, cardiac magnetic resonance, endocardial biopsy and echocardiography. The novel echocardiographic technique including tissue Doppler imaging, strain and strain rate imaging, speckle tracking echocardiography and real-time 3-dimensional echocardiography were involved in detail. © 2016, Editorial Department of Fudan University Journal of Medical Sciences. All right reserved.

Zhou N.-W.,Institute of Medical Imaging | Zhou N.-W.,Shanghai Institute of Cardiovascular Disease | Pan C.-Z.,Institute of Medical Imaging | Pan C.-Z.,Shanghai Institute of Cardiovascular Disease | And 8 more authors.
Fudan University Journal of Medical Sciences | Year: 2016

To assess early left heart functional alteration in patients with heart transplantaion by three-dimensional speckle-tracking echocardiography. Three-dimensional images of 55 patients with heart transplantaion were analyzed using post-processing software to evaluate and compare the left ventricular systolic function, volume and other indicators at 1 and 12 months after surgery. Fifty-five patients with heart transplantaion, with 2 cases lost to follow-up, were divided into the rejection group (n=10) and the no-rejection group (n=43). Intragroup comparison showed no difference for left ventricular function between 1 month and 12 months after transplantation in the no-rejection group while in the rejection group, left ventricular end diastolic volume (LVEDV), left ventricular end-stolic volume (LVESV), left ventricular stroke volume (LVSV), MASS at 12 months after transplantation were smaller than that at 1 month. Compared with the no-rejection group, the rejection group had similar left ventricular function at 1 month after heart transplantation, while at 12 months after surgery, their LVEDV, LVSV, MASS, and global principal longitudinal strain (GPSL) were significantly decreased (P<0.05). The left ventricular function had changed early after heart transplantation. Three-dimensional speckle-tracking echocardiography can accurately determine the alteration of left ventricular function in patients with heart transplantaion. © 2016, Fudan University. All right reserved.

Zhu K.,Fudan University | Zhu K.,Shanghai Institute of cardiovascular Disease | Li J.,Fudan University | Li J.,Shanghai Institute of cardiovascular Disease | And 8 more authors.
International Journal of Nanomedicine | Year: 2014

Induced pluripotent stem cells (iPSCs) have attracted keen interest in regenerative medicine. The generation of iPSCs from somatic cells can be achieved by the delivery of defned transcription factor (Oct4, Sox2, Klf4, and c-Myc[OSKM]). However, most instances of iPSC-generation have been achieved by potentially harmful genome-integrating viral vectors. Here we report the generation of iPSCs from mouse embryonic fibroblasts (MEFs) using arginine-terminated generation 4 polyamidoamine (G4Arg) nanoparticles as a nonviral trans-fection vector for the delivery of a single plasmid construct carrying OSKM (pOSKM). Our results showed that G4Arg nanoparticles delivered pOSKM into MEFs at a significantly higher transfection efficiency than did conventional transfection reagents. After serial transfections of pOSKM-encapsulated G4Arg nanoparticles, we successfully generated iPSCs from MEFs. Our study demonstrates that G4Arg nanoparticles may be a promising candidate for generating of virus-free iPSCs that have great potential for clinical application. © 2014 Zhu et al.

Zhu K.,Fudan University | Zhu K.,Shanghai Institute of Cardiovascular Disease | Li J.,Fudan University | Li J.,Shanghai Institute of Cardiovascular Disease | And 6 more authors.
Stem Cells International | Year: 2016

Stem cell therapy has attracted increasing attention as a promising treatment strategy for cardiac repair in ischemic heart disease. Nanoparticles (NPs), with their superior physical and chemical properties, have been widely utilized to assist stem cell therapy. With the help of NPs, stem cells can be genetically engineered for enhanced paracrine profile. To further understand the fate and behaviors of stem cells in ischemic myocardium, imaging NPs can label stem cells and be tracked in vivo under multiple modalities. Besides that, NPs can also be used to enhance stem cell retention in myocardium. These facts have raised efforts on the development of more intelligent and multifunctional NPs for cellular application. Herein, an overview of the applications of NPs-assisted stem cell therapy is given. Key issues and future prospects are also critically addressed. © 2016 Kai Zhu et al.

Hu K.,Fudan University | Hu K.,Shanghai Institute of Cardiovascular Disease | Hong T.,Fudan University | Hong T.,Shanghai Institute of Cardiovascular Disease | Hong T.,Shanghai Key Laboratory of Organ Transplantation
Fudan University Journal of Medical Sciences | Year: 2013

Endothelial progenitor cells (EPCs) have the ability to differentiate into mature endothelial cells (ECs). They can facilitate the repair of damaged blood vessels or augment development of new vessels. Recent data indicate that EPCs play a significant role in the occurrence and development of cardiac allograft vasculopathy (CAV). The injury of endothelium of donor heart post-transplantation triggers the process of neoendothelialization. This paper reviews homing and differentiation of EPCs in the occurrence and development of allograft vasculopathy, and explore the prediction, diagnosis and therapeutic value of EPCs on CAV.

Zhu K.,Fudan University | Zhu K.,Shanghai Institute of Cardiovascular Disease | Li J.,Fudan University | Li J.,Shanghai Institute of Cardiovascular Disease | And 10 more authors.
Archives of Medical Research | Year: 2015

Background and Aims: Intramyocardial autologous bone marrow-derived stem cells injection (IM-BMCs) has been used in patients with ischemic heart disease (IHD) who are ineligible for revascularization; however, the procedure has yielded mixed results. The objective of this meta-analysis was to evaluate the safety and therapeutic benefits of this treatment on a relatively large scale. Methods: PubMed, EMBASE, and Cochrane Library databases through September 2014 were searched for randomized clinical trials (RCTs) of IM-BMCs to treat IHD. Outcome measures were defined as mortality after treatment, change in left ventricular ejection fraction (LVEF), left ventricular end-systolic volume (LVESV) and left ventricular end-diastolic volume (LVEDV). Weighted mean differences for the changes were estimated with a random-effects model. Results: Nine RCTs were eligible for inclusion. IM-BMCs significantly reduced the risk of mortality (RR, 0.33; 95% CI, 0.17-0.65; p = 0.001). IM-BMCs significantly improved LVEF by 2.57% (95% CI, 0.34-4.80%; p = 0.02) and reduced LVESV by 9.67 mL (95% CI, -16.43 mL to -2.91 mL; p = 0.005). No significant improvement in LVEDV (WMD = 4.73 mL; 95% CI, -7.22 mL to 16.68 mL; p = 0.44) was detected in patients who received IM-BMC therapy. Conclusions: IM-BMC therapy showed clinical safety while being used as stand-alone treatment in IHD with no option of revascularization. The therapeutic efficacy requires further confirmation in large well-powered trials with long-term follow-up. © 2015 IMSS.

Zhu K.,Fudan University | Zhu K.,Shanghai Institute of Cardiovascular Disease | Wu M.,CAS Shanghai Institute of Ceramics | Lai H.,Fudan University | And 11 more authors.
Biomaterials | Year: 2016

Transplantation of gene-transfected bone marrow-derived mesenchymal stem cells (BMMSCs) is a promising strategy for ischemic myocardium repair, but current therapeutic strategy suffers from high toxicity and inefficient gene transfection in primary BMMSCs. Here we designed and synthesized molecularly organic-inorganic hybrid hollow mesoporous organosilica nanoparticles (HMONs) based on nano-synthetic chemistry, which are featured with concurrent large pore size over 20 nm, small particulate size, hollow cavity and high dispersity for gene transfection in BMMSCs and subsequent in vivo cardiac repair. To efficiently create the therapeutic gene-transfected stem-cell lines, hepatocyte growth factor (HGF) gene was applied to transfect BMMSCs via biocompatible surface-engineered HMONs as a high-performance gene-delivery nanosystem. On the rat model of myocardial infarction, transplantation of HGF gene-transfected BMMSCs enables the largely decreased apoptotic cardiomyocytes, reduced infarct scar size, relieved interstitial fibrosis, and increased angiogenesis in myocardium. The resultant cardiac repair further promotes the significant improvement of heart function. Therefore, the fabricated organic-inorganic hybrid HMONs with large pore size represent a generalizable strategy and platform for gene transfection in BMMSCs and further regenerative medicine. © 2015 Elsevier Ltd.

PubMed | Fudan University, Tongji University and Shanghai Institute of Cardiovascular Disease
Type: | Journal: Journal of cardiothoracic surgery | Year: 2016

Mild preoperative renal insufficiency is not rare in patients receiving isolated off-pump coronary artery bypass grafting surgery (OPCAB) surgery. However, there is less study aimed to evaluate the impact of mild preoperative renal insufficiency on in-hospital and follow-up outcomes after isolated OPCAB surgery. This single-centre, retrospective propensity score matching study aimed to evaluate the impact of mild preoperative renal insufficiency on in-hospital and long-term outcomes after first isolated OPCAB surgery.After propensity score matching, 1236 patients with preoperative estimated glomerular filtration rate (eGFR) of more than 60 ml/min/1.73 m(2) undergoing first isolated OPCAB surgery from January 2007 to December 2011 were entered into this study and were divided to normal group (eGFR90 ml/min/1.73 m(2), n = 618) and mild group (eGFR of 60-89 ml/min/1.73 m(2), n = 618). The in-hospital and long-term outcomes were investigated and retrospectively analyzed.The 2 propensity score-matched groups had similar baseline and procedural characteristics except the baseline eGFR. Thirty-five patients died during the same hospitalization or within 30 days of operation, with a surgical mortality of 2.8 %. Sixty-seven patients died during follow-up, with a long-term survival of 94.1 %. Univariate factor analysis showed that the 2 propensity score-matched groups have similar rates among in-hospital outcomes. Kaplan-Meier curves displayed a similar in-hospital survival between the 2 groups ((2) = 0.728, p = 0.393), while a better long-term survival in patients with normal preoperative renal function compared with mild preoperative renal insufficiency ((2) = 4.722, p = 0.030). After Cox proportional model was used, the hazard ratio for long-term mortality in patients with mild preoperative renal insufficiency compared with normal preoperative renal function was 1.72 (95 % CI 1.06-2.83, p = 0.032).Mild preoperative renal insufficiency compared with normal preoperative renal function reduced long-term survival, without evidence of worse in-hospital outcomes.

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