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Xie Y.-Q.,Shanghai University | Xie Y.-Q.,Shanghai Huangpu Center Hospital | Shi J.-Y.,Shanghai University | Li X.-Z.,Shanghai University | Sui J.-K.,Shanghai University
Academic Journal of Second Military Medical University

GATA3 transcription factor plays an important role in the growth and differentiation of normal breast tissues, and it is also closely related to the tumorigenesis of breast cancer. The roles of GATA3 vary in different breast cancer subtypes. This paper reviews the relationship of GATA3 with normal breast tissue and the tumorigenesis of breast cancer, in an attempt to provide theoretical evidences for future clinical applications of GATA3 in breast cancer. Source

Li Y.,Shanghai JiaoTong University | Yi H.,Shanghai Huangpu Center Hospital | Yao Y.,Shanghai JiaoTong University | Liao X.,Shanghai JiaoTong University | And 11 more authors.

MUC1 is an oncoprotein that is overexpressed in up to 90% of breast carcinomas. A previous in vitro study by our group demonstrated that the cytoplasmic domain of MUC1 (MUC1-CD), the minimal functional unit of MUC1, contributes to the malignant phenotype in cells by binding directly to β-catenin and protecting β-catenin from GSK3β-induced degradation. To understand the in vivo role of MUC1-CD in breast development, we generated a MUC1-CD transgenic mouse model under the control of the MMTV promoter in a C57BL/6J background, which is more resistant to breast tumor. We show that the expression of MUC1-CD in luminal epithelial cells of the mammary gland induced a hyperplasia phenotype characterized by the development of hyper-branching and extensive lobuloalveoli in transgenic mice. In addition to this hyperplasia, there was a marked increase in cellular proliferation in the mouse mammary gland. We further show that MUC1-CD induces nuclear localization of β-catenin, which is associated with a significant increase of β-catenin activity, as shown by the elevated expression of cyclin D1 and c-Myc in MMTV-MUC1-CD mice. Consistent with this finding, we observed that overexpression of MUC1-C is associated with β-catenin nuclear localization in tumor tissues and increased expression of Cyclin D1 and c-Myc in breast carcinoma specimens. Collectively, our data indicate a critical role for MUC1-CD in the development of mammary gland preneoplasia and tumorigenesis, suggesting MUC1-CD as a potential target for the diagnosis and chemoprevention of human breast cancer. © 2011 Li et al. Source

Zhao W.Y.,Shanghai JiaoTong University | Zhao W.Y.,Shanghai Huangpu Center Hospital | Shen K.W.,Shanghai JiaoTong University | Shen K.W.,Shanghai Huangpu Center Hospital | And 8 more authors.
Chinese Journal of Cancer Biotherapy

Objective: To investigate the infiltration of Foxp3 + cells and expression of Foxp3 protein in breast carcinoma tissues and their clinical significance. Methods: One hundred and sixty breast carcinoma and 22 paracancerous tissues (who had been diagnosed in Shanghai Huangpu Center Hospital from Jan. 2005 to Dec. 2005) were included in present study. The infiltration of Foxp3 + cells and expression of Foxp3 protein in breast carcinoma and paracancerous tissues were detected by immunohistochemistry, and their relationships to clinicopathologic features and expression of ER,PR,P53,Bcl 2,HER 2 in breast carcinoma were analyzed. Results: Both positive rate of Foxp3 + cell infiltration in mesenchyma tissues and Foxp3 protein in parenchyma tissues were significantly higher than those in normal paracancerous tissues (P<0.05), but there was no correlation between the Foxp3 + cell infiltration and Foxp3 protein expression in breast carcinoma tissues (P>0.05). Foxp3 + cell infiltration in mesenchyma tissues was positively correlated to lymph node metastasis, histological grade and P53 overexpression (P<0.05); Foxp3 protein expression in parenchyma tissues was positively correlated to lymph node metastasis, histological grade, pTNM stage and P53 overexpression (P<0.05), but not to overexpression of ER, PR, HER 2 and Bcl 2 (P>0.05). Univariate survival analysis indicated that Foxp3 expression in breast carcinoma was associated with 5 year overall survival rate, while multivariate analysis indicated that Foxp3 expression was not an independent prognostic factor for 5 year overall survival rate in breast carcinoma (P>0.05). Conclusion: Foxp3 protein is overexpressed in breast carcinoma tissues, which might be a potential bio marker but not an independent prognostic factor in breast carcinoma. Source

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