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Xu Y.-X.,Shanghai University | Xu Y.-X.,Shanghai Health Vocational and Technical College | Xiang Z.-B.,Chongqing University of Posts and Telecommunications | Chen X.-J.,Shanghai Health Vocational and Technical College | Chen H.S.,Shanghai University
Academic Journal of Second Military Medical University | Year: 2011

Objective: To study the anti-tumor constituents from the roots of Actinidia valvata Dunn. Methods: Column chromatographic techniques were used to isolate and purify the chemical constituents of the plant and their structures were elucidated by spectroscopic analysis, including 1HNMR, 13CNMR and MS. The cytotoxic activities of some individual compounds were examined by MTT assay. Results: Ten triterpenoids: 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid(1), asiatic acid(2), corosolic acid(3), 2α, 3α, 23, 24-tetrahydroxyurs-12-en-28-oic acid(4), 2α, 3α, 13β, 24-tetrahydroxyurs-11-en-28-oic acid-13-lactone(5), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid(6), 2α, 3α, 19α, 24-tetrahydroxyurs-12-en-28-oic acid(7), 2α, 3α, 24-trihydroxyurs-12, 20(30)-dien-28-oic acid(8), 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid(9), and oleanolic acid (10), and one plant sterol β-citosterol(11) were isolated from the CHCl3 fraction of the roots of Actinidia valvata Dunn. The cytotoxic activities of five compounds (1, 2, 3, 4, and 7) against A549, LOVO and HepG2 cell lines were evaluated. Conclusion: Compounds 3-8 are isolated from this plant for the first time. Compounds 2 and 3 possess cytotoxic activity against LOVO and HepG2 cell lines, and the cytotoxic activity decreases with the increase of polarity of individual compound.


Liu L.,Shanghai Health Vocational and Technical College | Wang W.-J.,Shanghai JiaoTong University | Zhu H.,Shanghai JiaoTong University | Li Z.-H.,Shanghai Health Vocational and Technical College | Ding W.-L.,Shanghai JiaoTong University
Acta Anatomica Sinica | Year: 2010

Objective: To investigate the effect of oxidative stress on diabetic erectile dysfunction (ED). Methods: Diabetes mellitus(DM) was induced by streptozotocin (STZ) injection. Eight weeks and twelve weeks later, penis erectile function was detected by injecting apomorphine. The concentrations of malondialdehyde (MDA) and total anti-oxygen capability (T-AOC) in penis were measured. Western blotting was used to measure nuclear factor E2-related factor 2(Nrf2) in both control and diabetic rat penis tissues in present study. Results: Compared with control group, the erection times of DM rats decreased significantly. The mean level of MDA in penis of DM rats were higher than that of control group(P < 0.01), and the mean level of T-AOC in DM rats was lower than that of control(P < 0.05). Nrf2 protein expression decreased in DM groups compared with that of control (P < 0.05). Conclusion: Oxidative stress is probably involved in the mechanism inducing diabetic ED.


Gu L.,East China Normal University | Liang Y.,Shanghai Health Vocational and Technical College | Zhou T.,East China Normal University | Tang X.,East China Normal University | Shi G.,East China Normal University
Analytical Methods | Year: 2012

Boronic acid functionalized multi-walled carbon nanotubes (MWCNTs) were prepared and used for sensitive and selective astragaloside IV determination through electrochemical methods using Alizarin Red S (ARS) as the current indicator. © The Royal Society of Chemistry 2012.


Xu X.-L.,Nantong University | Huang Y.-J.,Nantong University | Huang Y.-J.,Shanghai Health Vocational and Technical College | Ling D.-Y.,Nantong University | Zhang W.,Nantong University
Chinese Journal of Integrative Medicine | Year: 2015

Objective: To investigate the effect of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an active component extracted from the root of Polygonum multiflorum, on angiotensin II (Ang II)-induced proliferation of cultured rat vascular smooth muscle cells (VSMCs) and to identify the potential mechanism. Methods: Cell proliferation and cell cycle were determined by cell counting, 5-bromo-2′-deoxyuridine incorporation assay, proliferating cell nuclear antigen protein expression and flow cytometry. Levels of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2), mitogenic extracellular kinase 1/2 (MEK1/2) and Src in VSMCs were measured by Western blot. The expression of c-fos, c-jun and c-myc mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Intracellular reactive oxygen species (ROS) was measured by fluorescence assay. Results: TSG significantly inhibited Ang II-induced VSMCs proliferation and arrested cells in the G /S checkpoint (P<0.05 or P<0.01). TSG decreased the levels of phosphorylated ERK1/2, MEK1/2 and Src in VSMCs (P<0.05 or P<0.01). TSG also suppressed c-fos, c-jun and c-myc mRNA expression <0.05 or P<0.01). In addition, the intracellular ROS was reduced by TSG (P<0.01). Conclusions: TSG inhibited Ang II-induced VSMCs proliferation. Its antiproliferative effect might be associated with down-regulation of intracellular ROS, followed by the suppression of the Src-MEK1/2-ERK1/2 signal pathway, and hence, blocking cell cycle progression. © 2015, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.


Gu L.,East China Normal University | Jiang X.,East China Normal University | Liang Y.,Shanghai Health Vocational and Technical College | Zhou T.,East China Normal University | Shi G.,East China Normal University
Analyst | Year: 2013

In this work, we report a competitive sensor performing double recognition for the specific capture of dopamine (DA) with the combination of boronic acid functional groups and molecularly imprinted cavities based on poly(aniline-co-anthranilic acid) (PANANA) as the support material. This novel imprinting receptor bearing a covalent ester linkage to DA via boronic acid functional groups was prepared and applied as a recognition element in the construction of the electrochemical sensor. For the first time, aminophenylboronic acids (APBAs) and vinyl groups were both introduced onto the surface of PANANA nanomaterials. Then, selective copolymerization of acrylamide and ethylene glycol dimethacrylate (EGDMA) in the presence of the template DA was further achieved at the surface of APBA and vinyl group functionalized PANANA. The double recognition through the functional groups (boronic acids) and the shape of the cavities endowed this sensor with a specific affinity for the template DA in the presence of other interferents, such as norepinephrine (NE), epinephrine (EP), ascorbic acid (AA) and uric acid (UA). Moreover, the results obtained from a series of electrochemical experiments proved that this receptor had a good adsorption capacity and a fast mass transfer rate for DA. Thus, a novel electrochemical sensor with good selectivity and sensitivity was constructed with a linear response to the DA concentration in the range from 1.0 × 10-8 to 1.0 × 10-5 M and a detection limit of 3.33 × 10-9 M (S/N = 3). Besides, this novel electrochemical sensor was successfully applied to the detection of DA in DA injected and human plasma samples. © 2013 The Royal Society of Chemistry.


Zheng H.,East China University of Science and Technology | Wang Y.,Shanghai Health Vocational and Technical College | Zhong W.,East China University of Science and Technology
Proceedings - 2nd International Conference on Cloud and Green Computing and 2nd International Conference on Social Computing and Its Applications, CGC/SCA 2012 | Year: 2012

The current standard of the wireless meters reading interface is used to read wireless meters data and controls the system remotely. Interfaces are often asynchronous since their purpose is for connecting multiple distributed modules without common clock and it uses instructions based command execution method for interaction. The Petri net is a powerful modeling tool for describing and studying systems, analyzing of many properties and problems associated with concurrent systems. The workflow process of instruction based command execution process in digital home wireless meter reading interface is modeled and analyzed by Petri nets. Consequently the correctness of the workflow is verified. Also, suggested modification that can be done in the workflow process, considering the priority to the urgent commands to take actions immediately without any delay. The paper gives a solution for the modification of process. © 2012 IEEE.


Dai Z.,Fudan University | Jin Y.,Shanghai Health Vocational and Technical College
Oncology Reports | Year: 2013

Deleted in liver cancer-1 (DLC-1), a candidate tumor suppressor gene which is inactive in liver carcinogenesis, is located at 8p21.3, where deletions are frequently found in several types of human cancer. Promoter hypermethylation is an epigenetic mechanism leading to silencing of the gene expression, which may be the primary cause for the absence of DLC-1. We investigated the expression of the DLC-1 gene and the methylation of the DLC-1 gene in colon cancer cell lines (Caco-2, LoVo and HT-29). The data showed that reduced or undetectable levels of DLC-1 mRNA were found in HT-29 by reverse transcription-polymerase chain reaction (RT-PCR). By contrast, the DLC-1 gene was significantly expressed in Caco-2 and LoVo cells. These findings were in agreement with the data obtained from western blot analysis. To further determine whether aberrant methylation is a contributing factor to transcriptional inactivation of DLC-1 in HT-29, the methylation of promoter was examined using methylation-specific PCR and sodium bisulfite genomic sequencing in LoVo and HT-29 cells, which suggests that promoter hypermethylation accounts for silencing of the DLC-1 gene in HT-29 cells. Since DLC-1 is a candidate tumor suppressor gene, we sought to determine whether DLC-1 expression is associated with cell proliferation in colon cancer cell lines. RNA interference techniques were adopted to inhibit DLC-1 expression in the LoVo cell line and resulted in inhibition of cell growth and reduced colony formation. Collectively, our observations suggest that hypermethylation is responsible for abrogating the function of the DLC-1 gene in colon cancer and indicate a role of DLC-1 in colon carcinogenesis.

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