Shanghai Eighth Peoples Hospital

Shanghai, China

Shanghai Eighth Peoples Hospital

Shanghai, China
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Fu J.-L.,Shanghai JiaoTong University | Liu Y.,Shanghai Eighth Peoples Hospital | Li Y.-M.,Shanghai JiaoTong University | Chang C.,Shanghai JiaoTong University | Li W.-B.,Shanghai JiaoTong University
Journal of Neuroimaging | Year: 2014

BACKGROUND AND PURPOSE: Patients with amnestic mild cognitive impairment (aMCI) are at risk of developing Alzheimer's disease (AD). It is therefore important to identify biomarkers of conversion to AD. This study examined whether the integrity of white matter can predict this conversion. METHODS: Magnetic resonance imaging (MRI), diffusion tensor imaging (DTI) and neuropsychological features of aMCI subjects (n = 41) were compared with normal controls (n = 20) for 12-36 months. RESULTS: Compared to controls, 22 aMCI subjects had lower fractional anisotropy (FA) values in the cingulate fasciculus (CF) at baseline, and 19 of those converted to AD during follow-up. Only two of the other 19 aMCI patients converted to AD. Compared to baseline, AD converters showed lower FA values in the anterior frontal lobe, temporal lobe, hippocampus, inferior fronto-occipital fascicles, corpus callosum genu and CF, and higher apparent diffusion coefficient values in the temporal lobe and hippocampus. CONCLUSIONS: Those aMCI subjects with lower than normal FA values in the CF were more likely to convert to AD. The connectivity of the hippocampus and cingulate bundles may be affected in the early stage of AD. Impairment of white matter and fiber bundles was more severe at the AD stage than the aMCI stage. © 2013 by the American Society of Neuroimaging.


Hong J.,Shanghai Putuo District Center Hospital | Sun J.,Shanghai Zhabei District Shibei Hospital | Huang T.,Shanghai Eighth Peoples Hospital
BioMed Research International | Year: 2013

Aim. To detect the expression pattern of tricho-rhino-phalangeal syndrome-1 (TRPS1) in human colon cancer and to analyze its correlation with prognosis of patients with this disease. Methods. The expressions of TRPS1 in human colon cancer and its corresponding noncancerous colon tissues were detected at both mRNA and protein levels. Results. The mRNA and protein expression levels of TRPS1 were both significantly higher in colon cancer than in corresponding noncancerous colon tissues (both P<0.001). The protein level of TRPS1 in colon cancer tissues was significantly correlated with the mRNA level (r=0.9, P<0.001). Additionally, immunohistochemistry analysis also found increased TRPS1 expression in 63.0% (63/100) of colon cancer tissues. High TRPS1 expression was significantly associated with positive lymph node metastasis (P=0.006) and higher pathological stage (P=0.008) of patients with colon cancer. Multivariate Cox regression analysis further suggested that the increased expression of TRPS1 was an independent poor prognostic factor for this disease. Conclusion. Our data offer the convincing evidence for the first time that the increased expression of TRPS1 may be involved in the pathogenesis and progression of colon cancer. TRPS1 might be a potential marker to predict the prognosis in colon cancer. © 2013 Jun Hong et al.


Li Z.,Nanjing Agricultural University | Li Z.,Washington University in St. Louis | Wu S.,Shanghai Eighth Peoples Hospital | Ye C.,Nanjing Agricultural University
Journal of Raman Spectroscopy | Year: 2015

The central region of the hypermineralized dolphin's bulla (ear bone), contains >95wt% mineral with ~10wt% structurally incorporated carbonate. The mineral-bioapatite undergoes phase changes during heating. However, the step-by-step changes with increasing temperature are still unclear. Raman spectroscopy is proved to be a powerful technique in analyzing crystallographic transformation of bioapatite. The bulla was heated stepwise from 100°C to 900°C for the Raman analyses. Both positions and widths of Raman peaks were studied. Crystals of the bioapatite are stable up to 200°C. From 300°C to 600°C, the decrystallization is a gradual process with delicate changes rather than a sharp transformation. Carbonate is depleted at ~500°C, and then decrystallization is terminated. At temperature over 600°C, bioapatite re-crystallizes and is transformed to OHAp at 700°C. The crystallinity is then gradually improved while the temperature increased to 900°C. No phases of β-tricalcium phosphate or calcium pyrophosphate were detected in this study. Copyright © 2015 John Wiley & Sons, Ltd.


Li F.Q.,Shanghai Eighth Peoples Hospital
International journal of nanomedicine | Year: 2011

Scopolamine hydrobromide (SH)-loaded microparticles were prepared from a colloidal fluid containing ionotropic-gelated chitosan nanoparticles using a spray-drying method. The spray-dried microparticles were then formulated into orally disintegrating tablets (ODTs) using a wet granulation tablet formation process. A drug entrapment efficiency of about 90% (w/w) and loading capacity of 20% (w/w) were achieved for the microparticles, which ranged from 2 μm to 8 μm in diameter. Results of disintegration tests showed that the formulated ODTs could be completely dissolved within 45 seconds. Drug dissolution profiles suggested that SH is released more slowly from tablets made using the microencapsulation process compared with tablets containing SH that is free or in the form of nanoparticles. The time it took for 90% of the drug to be released increased significantly from 3 minutes for conventional ODTs to 90 minutes for ODTs with crosslinked microparticles. Compared with ODTs made with noncrosslinked microparticles, it was thus possible to achieve an even lower drug release rate using tablets with appropriate chitosan crosslinking. Results obtained indicate that the development of new ODTs designed with crosslinked microparticles might be a rational way to overcome the unwanted taste of conventional ODTs and the side effects related to SH's intrinsic characteristics.


Qin X.-J.,Shanghai Eighth Peoples Hospital | Ling B.X.,Stanford University
Oncology Letters | Year: 2012

Breast cancer is one of the most common types of invasive cancer in females worldwide. Despite major advances in early cancer detection and emerging therapeutic strategies, further improvement has to be achieved for precise diagnosis to reduce the chance of metastasis and relapses. Recent proteomic technologies have offered a promising opportunity for the identification of new breast cancer biomarkers. Matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS) and the derived surface-enhanced laser desorption/ionization mass spectrometry (SELDI-TOF MS) enable the development of high-throughput proteome analysis based on comprehensive reliable biomarkers. In this review, we examined proteomic technologies and their applications, and provided focus on the proteomics-based profiling analyses of tumor tissues/cells in order to identify and confirm novel biomarkers of breast cancer.


Yang X.-J.,Shanghai Eighth Peoples Hospital | Xing G.-F.,Shanghai Eighth Peoples Hospital
CardioVascular and Interventional Radiology | Year: 2015

Objective: To describe a new interventional technique to remove foreign bodies (FBs) embedded in soft tissues around vital vessels. Methods: Under fluoroscopic guidance and using local anesthesia, percutaneous removal of FBs was performed using forceps in nine patients. All patients suffered from a metallic soft tissue FB located in close proximity to important vessels and one also had a small traumatic pseudoaneurysm adjacent to the FB. Prior to removal of the FB, the position of the nearest vessel was identified using a guide wire or catheter placed into the vessel. Balloon catheter was also simultaneously used to temporarily stop the blood flow of the nearest artery during the FB removal in three of the nine patients. Results: All of the nine FBs with 0–2 mm interval to the nearest vessel were successfully removed in the nine patients without any serious complications. The removed FBs measured 3–12 mm in length and 1–3 mm in width. The total fluoroscopic time of retrieval of each FB was 5–9 min (mean, 6.4 min). The volume of intraoperative bleeding ranged from 5 to 12 ml (mean, 7.5 ml). The length of hospital stay for each patient ranged from 4 to 8 days (mean, 5.5 days). Conclusion: Vascular intervention-aided percutaneous FB removal is minimally invasive and an effective method for removal of FBs around vital vessels. © 2014, Springer Science+Business Media New York and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE).


Yang X.-J.,Shanghai Eighth Peoples Hospital | Xing G.-F.,Shanghai Eighth Peoples Hospital | Shi C.-W.,Shanghai Eighth Peoples Hospital | Li W.,Shanghai Eighth Peoples Hospital
Korean Journal of Radiology | Year: 2013

Objective: To investigate the value of 3-dimensional (3D) CT virtual anatomy imaging (VAI) in the complex foreign body (FB) retrieval of the soft tissues. Materials and Methods: Four hundred and seventy-five patients with radiopaque FB(s) diagnosed by radiograph underwent contrast-enhanced 3D CT examination. VAI was reconstructed by volume-rendering opacity software, by sliding down the lowest threshold from -600 to 100 HU. The imaging was grouped into three groups: A (axial and multi-planar reformation [MPR] images), B (standard 3D imaging with axial and MPR images), and C (VAI with axial and MPR images). They were analyzed to reveal the type, size, number, location, complications, and the interventional removability of the object, with the comparisons in the management and clinical outcomes on the patient follow-up studies. The data were subjected to chi-square tests, with p value < 0.05 indicating significant statistical difference. Results: The FB shape, size, number, site distribution and vessels around FB, as well as the FB-associated vascular complications and the FB interventional removability were assessed more accurately in Group C than in Group B or Group A (p < 0.005). There was no significant difference in disclosing the type and depth of the FB among the three groups (p > 0.75). On the basis of the 3D CT, especially the enhanced 3D CT VAI, the followings were processed: the recommendation of interventional removal in 286 (60.47%) and non-intervention in 187 (39.53%) of the 473 patients with soft-tissue FB(s); in 352 (56.50%) of the 623 radiopaque FBs patients, 258 (54.55%) patients accurately detected on 3D CT and the successful removal by intervention (343 FBs) or surgery (9 FBs) without any sequela; and 215 (45.45%) patients with 271 FBs lost in the follow-up, with their departure from the hospital. Conclusion: The 3D CT, especially 3D enhanced CT VAI, has great incremental value in further diagnosis and management of complex FB extraction from soft tissues.


Xing G.-F.,Shanghai Eighth Peoples Hospital | Shi C.-W.,Shanghai Eighth Peoples Hospital | Qian H.-X.,Shanghai Eighth Peoples Hospital | Qin X.-J.,Shanghai Eighth Peoples Hospital
Journal of Surgical Research | Year: 2013

Background: We studied methods of locating metallic foreign bodies in soft tissue of the human body. Methods: Using a three-dimensional (3D) locator, we removed metallic foreign bodies precisely from soft tissue of 7390 patients through magnetic forceps between June 1999 and June 2009. Results: In 7390 patients, we successfully removed 99.5% of all metallic foreign bodies by 3D locator and forceps. Average operation time was 5 min. Conclusions: Metallic foreign bodies can be located precisely and removed simply with few complications using our 3D location method. The method may lead to minor trauma, less suffering, and a high success rate. © 2013 Elsevier Inc. All rights reserved.


Xiao J.,Changzheng Hospital | Yang X.-J.,Shanghai Eighth Peoples Hospital | Yang X.-J.,Changzheng Hospital | Xiao X.-S.,Changzheng Hospital
Journal of Orthopaedic Trauma | Year: 2012

Objective: To investigate the early influence of traction on blood supply to the femur head after femoral neck fractures using digital subtraction angiography (DSA). Design: Prospective case series. Setting: University Level I trauma center. Patients: Nine patients who sustained a unilateral femoral neck fracture underwent selective femoral artery DSA within 2-23 days after their injury. Intervention: DSA of the medial and lateral circumflex femoral artery was performed for all 9 fractured hips before traction. Repeat DSA study of the femoral head circulation was performed after the addition of 3 kg of traction in 7 patients and 5 kg of traction in 6 patients. For comparison, DSA was also performed on the uninjured hip in 8 of the 9 patients. Main Outcome Measure: Blood circulation of the femoral head was evaluated by observing morphology of the feeding arteries, perfusion volume, venous drainage, and the circulation time of the microvasculature. Results: Femoral neck fracture damaged the retinaculum artery and led to femoral head hemodynamic disorder in all 9 cases. Application of linear traction and repeat DSA decreased femoral head perfusion (faint arterial imaging and delayed venous display) in all patients' retinaculum arteries except the inferior branch compared with the pretraction imaging. These findings were more pronounced as the traction was increased from 3 to 5 kg. These findings were significant (P < 0.05). Conclusions: Traction impairs blood perfusion to the femoral head. Blood flow in the retinacular arteries was reduced and venous drainage impeded. Traction may be one of the major causes of femoral head osteonecrosis after femoral neck fracture. Copyright © 2012 by Lippincott Williams & Wilkins.


Li X.-H.,Shanghai Eighth Peoples Hospital | Fang D.-N.,Shanghai Eighth Peoples Hospital | Zeng C.-M.,Shanghai Eighth Peoples Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2011

Abnormal gene transcription plays an important role in oncogenesis. In cancer cells, the improper activation of specific genes is usually ascribed to aberrant transcription machinery including transcription factors, RNA polymerase II, and Mediator complex. This study reports on short hairpin RNA (shRNA)-mediated gene silencing of MED19, a subunit of Mediator complex, and its effect on the growth of pancreatic cancer cells. RNA interference was performed by lentivirus shRNA system to specifically knockdown MED19 expression in Aspc-1 and Panc-1 cells. The knockdown efficiency of MED19 was confirmed by quantitative RT-PCR and western blot. The effect of MED19 shRNA on Aspc-1 and Panc-1 cell proliferation was evaluated by methylthiazoletetrazolium assay, BrdU incorporation assay, colony formation assay, and flow cytometry assay. This study shows that downregulation of MED19 remarkably reduced cancer cell proliferation and colony formation capacity in two pancreatic cancer cell lines. In addition, downregulated MED19 induced G1-phase cell cycle arrest and apoptosis. This study provides a potent role of MED19 in promoting pancreatic cancer growth and a possible drug target for cancer therapy. © 2011, Mary Ann Liebert, Inc. 2011.

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