Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding

Shanghai, China

Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding

Shanghai, China
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Luo G.,Shanghai Ocean University | Luo G.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Zhang N.,Shanghai Ocean University | Tan H.,Shanghai Ocean University | And 3 more authors.
Aquacultural Engineering | Year: 2017

With additional organic carbon, fish waste can be used as a substrate to produce bioflocs, a protein source for aquaculture animals. In choosing a carbon source, one should consider convenience, cost and biodegradability. This study investigates the efficiency of poly-β-hydroxybutyric acid (PHB), a biologically degradable polymer, as a carbon source to produce bioflocs in suspended growth bioreactors (SGRs), PHB-SGRs, compared with glucose (GLU-SGRs). The C:N ratio in PHB-SGRs could be maintained around 15:1. The volatile suspended solids (VSS) yield was 2.94 ± 0.72 gVSS/g fish waste for PHB-SGRS and 4.90 ± 0.23 gVSS/g fish waste for GLU-SGRs. The recycling rate of nitrogen in aquaculture solid waste was 56 ± 2% and 87 ± 7% for the PHB-SGRs and Glu-SGRs. No significant differences were found in the bioflocs produced and in the crude protein content of the produced bioflocs between PHB-SGRs and GLU-SGRs. PHB-SGRs and GLU-SGRs could remove dissolved inorganic nitrogen from aquaculture wastewater, with average values of 11.82 ± 8.95 and 16.27 ± 3.95 mg/g TSS/d. Because the calculation of the added amount of carbon and the multiple additions of carbon was avoided, PHB is considered to be a good choice as an organic carbon source for this process, even though not all parameters used for assessment were better than those of GLU-SGRs. © 2017 Elsevier B.V.


Luo G.,Shanghai Ocean University | Luo G.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Xu G.,Shanghai Ocean University | Tan H.,Shanghai Ocean University | And 3 more authors.
International Biodeterioration and Biodegradation | Year: 2016

Dissolved oxygen (DO) can inhibit the denitrifying process. Using polycaprolactone (PCL) as an organic carbon source and biofilm carrier (PCL-denitrifying process), an anoxic environment can be created to minimize the inhibition. This study investigates the effect of DO on the PCL-denitrifying process. Four DO conditions are established, ananoxic condition (group A, 0.44 ± 0.01 mg/L DO), a low DO condition (group B, 3.83 ± 0.22 mg/L DO), a moderate DO condition (group C, 6.12 ± 0.50 mg/L DO), and a high DO condition (group D, 10.45 ± 0.65 mg/L DO). This study demonstrates that the amount of released dissolved organic carbon is greater in the presence of DO compared to the anoxic condition. However, the amount of dissolved organic carbon released from PCL does not increase with an increase in DO. No significant differences in the nitrate nitrogen (NO3 --N) and total nitrogen (TN) removal efficiencies were between groups B and C. The NO3 --N and TN removal efficiencies in the group B and C were higher than those of group A and group D. The lowest NO3 --N and TN removal efficiencies were in the group D. These results indicate that the use of PCL as an organic carbon source and biofilm carrier can effectively minimize the inhibition of DO on the denitrifying process, although more than 10 mg/L DO in the water phase still inhibits this process to a certain degree. © 2016 Elsevier Ltd.


Wang L.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Li Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Chen Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Leng X.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | And 3 more authors.
Mitochondrial DNA | Year: 2015

In this study, the complete mitogenome sequence of Protomelas annectens Regan 1922 has been sequenced by next-generation sequencing method. The assembled mitogenome consisting of 16 583 bp, includes 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and 1 control region. The overall base composition of P. annectens is 27.41% for A, 30.07% for C, 15.88% for G, 26.64% for T and shows 94% identity to threadfin cichlid, Petrochromis trewavasae. These data will provide useful molecular information for phylogenetic relationships within the family Cichlidae species. © 2015 Taylor & Francis.


Zhao Y.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Chen Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Gao J.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Wang L.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | And 3 more authors.
Mitochondrial DNA | Year: 2015

In this study, the mitochondrial genome of Glossolepis incisus (Weber 1907) (Atheriniformes: Melanotaeniidae) was sequenced for the first time. The assembled mitogenome consisting of 16 529 bp, includes 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and 1 putative control region. The overall base composition of G. incisus is 27.51% for A, 30.04% for C, 16.06% for G, 26.39% for T and showS 91% identities to Lake Kutubu Rainbowfish, Melanotaenia lacustris. These data will provide useful molecular information for phylogenetic relationships within the family Melanotaeniidae species. © 2015 Taylor & Francis.


Li Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Chen Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Gao J.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Wang L.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | And 2 more authors.
Mitochondrial DNA | Year: 2015

In this study, the complete mitogenome sequence of the Symphysodon haraldi (Schultz, 1960) has been sequenced by next-generation sequencing method. The mitogenome consists of 16 543 bp, including 13 protein-coding genes, 22 transfer RNAs and 2 ribosomal RNAs genes. The overall base composition of the fish is 28.8% for A, 30.1% for C, 15.0% for G, and 26.1% for T, suggesting a 97% identity to S. discus Heckel. It provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for Symphysodon phylogeny. © 2015 Taylor & Francis.


Luo G.,Shanghai Ocean University | Luo G.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Xu G.,Shanghai Ocean University | Gao J.,Shanghai Ocean University | And 2 more authors.
Journal of Environmental Sciences (China) | Year: 2016

Nitrate-nitrogen (NO3 --N) always accumulates in commercial recirculating aquaculture systems (RASs) with aerobic nitrification units. The ability to reduce NO3 --N consistently and confidently could help RASs to become more sustainable. The rich dissolved oxygen (DO) content and sensitive organisms stocked in RASs increase the difficulty of denitrifying technology. A denitrifying process using biologically degradable polymers as an organic carbon source and biofilm carrier was proposed because of its space-efficient nature and strong ability to remove NO3 --N from RASs. The effect of dissolved oxygen (DO) levels on heterotrophic denitrification in fixed-film reactors filled with polycaprolactone (PCL) was explored in the current experiment. DO conditions in the influent of the denitrifying reactors were set up as follows: the anoxic treatment group (Group A, average DO concentration of 0.28±0.05mg/L), the low-oxygen treatment DO group (Group B, average DO concentration of 2.50±0.24mg/L) and the aerated treatment group (Group C, average DO concentration of 5.63±0.57mg/L). Feeding with 200mg/L of NO3 --N, the NO3 --N removal rates were 1.53, 1.60 and 1.42kg/m3 PCL/day in Groups A, B and C, respectively. No significant difference in NO3 --N removal rates was observed among the three treatments. It was concluded that the inhibitory effects of DO concentrations lower than 6mg/L on heterotrophic denitrification in the fixed-film reactors filled with PCL can be mitigated. © 2016.


Luo G.,Shanghai Ocean University | Luo G.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Wang J.,Shanghai Ocean University | Ma N.,Shanghai Ocean University | And 3 more authors.
Journal of Microbiology and Biotechnology | Year: 2016

Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. In the present study, 94% of geosmin and 97% of 2-MIB in suspended growth reactors producing bioflocs (SGRs) with aquaculture waste were removed after inoculation with Bacillus subtilis, significantly higher than that of control SGRs (70% of geosmin and 86.4% of 2-MIB). The lowest concentrations of geosmin and 2-MIB achieved in the effluent of the SGRs were 2.43 ± 0.42 ng/l and 2.23 ± 0.15 ng/l, respectively. The crude protein content of the bioflocs produced in the SGRs was 35 ± 4%. The NH4 +-N and NO2 --N concentrations in the effluent of the reactors were 1.13 ± 0.21 mg/l and 0.42 ± 0.04 mg/l, respectively. These results suggest that inoculated with Bacillus subtilis, SGRs have a better performance to reuse the nitrogen in fish waste and to remove geosmin and 2-MIB from the culture water efficiently. © 2016 by The Korean Society for Microbiology and Biotechnology.


Zhang N.,Shanghai Ocean University | Luo G.,Shanghai Ocean University | Luo G.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Tan H.,Shanghai Ocean University | And 3 more authors.
Aquaculture | Year: 2016

A 120-day experiment was conducted to investigate the efficiency of rearing genetically improved farmed tilapia in biofloc-based culture tanks with Poly-β-hydroxybutyric (PHB) acid as a carbon source. PHB was introduced into the culture tanks in the form of bagged granules (PHB-tanks). Glucose was added after each feeding to adjust the C:N ratio in the control group (Glu-tanks). Total ammonium nitrogen and nitrite nitrogen were maintained at levels lower than 2.0 mg L− 1 and 0.5 mg L− 1 in all tanks during the experimental period. Crude protein (CP) and crude lipid (CL) content of bioflocs produced in the PHB-tanks were 34.06 ± 0.69% and 6.58 ± 0.34%, respectively, which were not significantly different from those in the Glu-tanks. The initial stocking density was 3.27 kg m− 3 in all tanks and reached 37.93 kg m− 3 fish in the PHB-tanks and 44.14 kg m− 3 in the Glu-tanks at the end of the experiment, respectively. No significant differences were found in terms of survival rates, final stocking densities or food conversion rates between the two groups. The specific growth rates of tilapia (2.18%) and the final individual tilapia weights (192 ± 51.15 g fish− 1) in PHB-tanks were significantly lower than those in Glu-tanks. There were no significant differences in the CP and CL contents of fish dorsal muscle when compared between the two groups. Intestinal lipase activity in fish from the PHB-tanks was significantly lower than those from the Glu-tanks and total superoxide dismutase activity in the serum of fish in the PHB-tanks was significantly higher than fish from the Glu-tanks. No significant differences were found in a range of other digestive and immune parameters at the end of the experiment. The cost of PHB was US$ 0.21 ± 0.02 kg− 1, which was significantly lower during the experimental period than that associated with the Glu-tanks (US$ 1.69 ± 0.06 kg− 1). Collectively, our results showed that PHB is a favored and convenient carbon source for biofloc-based culture systems for tilapia. Statement of relevance The objective of the present study was motivated by the urgent need to develop a more favorite carbon source for biofloc-technology-based tilapia aquaculture systems. © 2016 Elsevier B.V.


Luo G.-Z.,Shanghai Ocean University | Luo G.-Z.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | Ma N.,Shanghai Ocean University | Ma N.,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding | And 5 more authors.
Scientific World Journal | Year: 2015

The effectiveness of carbohydrate addition and the use of ultrasonication as a pretreatment for the mesophilic anaerobic digestion of saline aquacultural sludge was assessed. Analyses were conducted using an anaerobic sequencing batch reactor (ASBR), which included stopped gas production attributed to the saline inhibition. After increasing the C: N ratio, gas production was observed, and the total chemical oxygen demand (TCOD) removal efficiency increased from 75% to 80%. The TCOD removal efficiency of the sonication period was approximately 85%, compared to 75% for the untreated waste. Ultrasonication of aquaculture sludge was also found to enhance the gas production rate and the TCOD removal efficiency. The average volatile fatty acid (VFA) to alkalinity ratios ranged from 0.1 to 0.05, confirming the stability of the digesters. Furthermore, soluble chemical oxygen demand (SCOD), VFA, and PO43 - concentrations increased in the effluents. There was a 114% greater gas generation during the ultrasonication period, with an average production of 0.08 g COD/L·day-1. © 2015 Guo-zhi Luo et al.

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