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Zhang Y.-P.,Shanghai Institute of Technology | Fan H.-W.,Shanghai Agriculture Technology Extension and Service Center | Yang S.-J.,Shanghai Institute of Technology | Chen Y.-Y.,Shanghai Institute of Technology
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

The experiment was carried out by 'Hamilü' (cadmium-tolerant) and 'Xiulü' (cadmium-sensitive) in climate chambers and substrates to investigate the alleviating effects of exogenous salicylic acid (SA) on melon seedlings for growth, photosynthesis and reactive oxygen metabolism under cadmium stress. The results showed that under Cd stress, melon-seedling growth, photosynthesis and chlorophyll contents were decreased, the antioxidant enzyme activities, proline, MDA content and O2 ̄ producing rate were increased. SA could increase melon-seedling growth, chlorophyll contents, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), stomatal limitation (Ls) and water use efficiency (WUE), while reduce intercellular carbon dioxide concentration (Ci) under cadmium stress. SA also raised leaf the activity of superoxid dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), proline and soluble protein content, reduced MDA content and O2 ̄ producing rate, suppressed the membrane lipid peroxidation under cadmium stress. Under cadmium stress condition, we not only found that SA caused enhancement in photosynthesis and antioxidant enzyme activity of two melon genotypes, but also found that the enhancement of cadmium-sensitive 'Xiulü' was higher than cadmium-tolerant 'Hamilü'. Obviously, SA was favorable for the seedlings to increase the growth and reactive oxygen metabolism, capture and converse solar energy, thus improving melon growth and abating the inhibitory effects of cadmium stress on melon, These findings suggested that ameliorative effect of SA on melon seedlings with cadmium stress was effective, especially for cadmium -sensitive genotypes.

Chen X.,Chinese Academy of Agricultural Sciences | Chen X.,Shanghai Agriculture Technology Extension and Service Center | Dong F.,Chinese Academy of Agricultural Sciences | Xu J.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Chirality | Year: 2015

The enantioselective degradation behavior of the chiral insecticide dinotefuran in cucumber and soil was investigated under greenhouse conditions based on the method established with a normal-phase high-performance chromatography (HPLC) on a ChromegaChiral CCA column (250 × 4.6 mm, 5 μm, ES Industries). The linearity range, matrix effect, precision, and accuracy of the method were evaluated and the method was then successfully applied for the enantioselective analysis of dinotefuran in cucumber and soil. Significant enantioselectivity of degradation was observed in soil according to the results. The (+)-dinotefuran was more persistent in soil with half-life of 21.7 d, which is much longer than that of (-)-dinotefuran (16.5 d). In cucumber, the (-)-dinotefuran also tended to be preferentially degraded both in foliar and douche treatment. However, the statistical analysis indicated the enantioselectivity of degradation in cucumber was not significant. The research provides the first report concerning the enantioselective degradation of dinotefuran enantiomers and the results can be used for understanding the insect-controlling effect and food safety evaluation. © 2014 Wiley Periodicals, Inc.

Ma L.,Shanghai Agriculture Technology Extension and Service Center | Chen J.,Shanghai Agriculture Technology Extension and Service Center | Zhao L.,Shanghai Agriculture Technology Extension and Service Center | Zhan X.,Shanghai Agriculture Technology Extension and Service Center
Chinese Journal of Chromatography (Se Pu) | Year: 2015

A solid phase extraction coupled with ultra high performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS) method was developed for simultaneous determination of six amide pesticides, cyantraniliprole, mandipropamid, boscalid, fluopicolide, thifluzamide and flubendiamide, in vegetables and fruits. After extraction with acetonitrile, purification with Florisil SPE cartridges and dissolution with methanol, the targets in the sample solutions were analyzed by UPLC-MS/MS on an Agilent Proshell 120 EC-C18 column with a mixture of 0. 1% formic acid solution and methanol as the mobile phases under gradient elution conditions. The mass spectrometer operated in multiple reaction monitoring (MRM) mode with the negative and positive modes. Good linearity was obtained for the six amide pesticides at the mass concentrations of 0. 000 5-1. 00mg/L with the correlation coefficients more than 0. 999. The fortified recoveries were in the range of 72. 4% -119. 4% with the concentration levels at 0. 01, 0. 1 and 1. 0 mg /kg for cyantraniliprole, mandipropamid, boscalid, fluopicolide, thifluzamide, and 0. 001, 0. 01 and 0.1 mg/kg for flubendiamide. The relative standard deviations (RSDs) were less than 15% and the limits of quantification were 0. 01 mg/kg for cyantraniliprole, mandipropamid, boscalid, fluopicolide, thifluzamide, and 0. 001 mg/kg for flubendiamide. All the above observations indicate that the established analytical method is simple, efficient and sensitive, and suitable for the determination of the six amide pesticides in vegetables and fruits.

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