Shanghai Agricultural Extension and Service Center

Shanghai, China

Shanghai Agricultural Extension and Service Center

Shanghai, China

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Liu K.,State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process | Liu K.,East China University of Science and Technology | Chen L.,Shanghai Fisheries Research Institute | Zhang W.,State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process | And 4 more authors.
Ecotoxicology | Year: 2015

Lead (Pb) and decabromodiphenyl ether (BDE209) are the main contaminants at e-waste recycling sites, and their potential toxicological effects on terrestrial organisms have received extensive attention. However, the impacts on the oxidative perturbations and hydroxyl radical (·OH) generation in earthworms of exposure to the two chemicals remain almost unknown. Therefore, indoor incubation tests were performed on control and contaminated soil samples to determine the effects of Pb in earthworms Eisenia fetida in the presence of BDE209 through the use of several biomarkers in microcosms. The results have demonstrated that the addition of BDE209 (1 or 10 mg kg−1) decreased the enzymatic activities [superoxide dismutase, catalase (CAT), peroxidase] and total antioxidant capacity (T-AOC) compared with exposure to BDE209 alone (50, 250 or 500 mg kg−1). Electron paramagnetic resonance spectra indicated that ·OH radicals in earthworms were significantly induced by Pb in the presence of BDE209. The changing pattern of malondialdehyde (MDA) contents was accordant with that of ·OH intensity suggested that reactive oxygen species might lead to cellular lipid peroxidation. Furthermore, CAT exhibited more sensitive response to single Pb exposure than the other biomarkers, while T-AOC, ·OH and MDA might be three most sensitive biomarkers in earthworms after simultaneous exposure to Pb and BDE209. The results of these observations suggested that oxidative stress appeared in E. fetida, and it may play an important role in inducing the Pb and BDE209 toxicity to earthworms. © 2014, Springer Science+Business Media New York.


Zhang W.,Laboratory of Environmental Risk Assessment and Control on Chemical Process | Zhang W.,East China University of Science and Technology | Chen L.,Laboratory of Environmental Risk Assessment and Control on Chemical Process | Chen L.,East China University of Science and Technology | And 7 more authors.
Environmental Toxicology and Pharmacology | Year: 2014

Decabromodiphenyl ether (BDE209) and tetrabromobisphenol A (TBBPA) are the main contaminants at e-waste recycling sites, and their potential toxicological effects have received extensive attention. However, the impact on soil culturable microbial population and enzyme activity of joint exposure to the two chemicals remains almost unknown. Therefore, indoor incubation tests were performed on control and contaminated soil samples to determine the eco-toxicological response in the joint presence of BDE209 and TBBPA for the first time. The results have demonstrated some notable toxic effects due to long-term exposure to either or both contaminants. The inhibition ratios of microbial populations increased with incubation time and increasing concentrations of BDE209 or TBBPA following certain dose-response relationships and time-effect trends. The response sensitivity sequence was fungi. >. bacteria. >. actinomycete. The influence of the two chemicals on soil enzymes reached peak values on day 7, and highly significant differences (P<. 0.01) were observed compared to the controls. Urease was more susceptive to the two chemicals than catalase and saccharase activities. Generally, the joint toxicity of both contaminants on soil microbes, catalase or saccharase activities indicated antagonistic effects, while, as for urease activity, addition role was dominant. Such observations have provided the useful information of potential ecological effects of brominated flame retardants contamination in the environment. © 2014 Elsevier B.V.


Zhu E.,Zhejiang University | Zhu E.,Shanghai Agricultural Extension and Service Center | Liu D.,Zhejiang University | Li J.G.,Zhejiang University | And 5 more authors.
Journal of Plant Nutrition | Year: 2011

We characterized the effect of nitrogen (N) fertilizer on root morphology, photosynthetic pigments and cadmium (Cd) accumulation in a series of hydroponic experiments by using hyperaccumulating ecotype of Sedum alfredii H. (HE) and non-hyperaccumulating ecotype (NHE) plants. Cd accumulation in both ecotypes increased with increase of N levels until it reached the peak at 16 mM and then decreased suddenly. Among all the three N forms tested, ammonium sulfate [(NH4)2SO4] was found to be an optimum choice. Based on these findings, a pot experiment was also conducted to evaluate the effect of three N fertilizers on phytoextraction from a slightly Cd-contaminated soil by the hyper-accumulating plant Sedum alfredii H. © Taylor & Francis Group, LLC.


Xie X.,Nanjing University | Gong S.,Zhejiang Economic and Trade Polytechnic | Wang X.,Nanjing University | Wu Y.,Nanjing University | Zhao L.,Shanghai Agricultural Extension and Service Center
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2011

A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2:1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r2 > 40.99) was obtained for three AVMs ranging from 0.01 to 5μg ml-1, i.e. 0.01-5.0 μg g-1 in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 μg g-1 and between 0.004 and 0.006 μg g-1, respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice. © 2011 Taylor & Francis.


Xie X.,Nanjing University | Wang X.,Nanjing University | Zhao L.,Shanghai Agricultural Extension and Service Center
Food Analytical Methods | Year: 2011

This paper describes a simplified high-performance liquid chromatography method without clean-up step for the determination of abamectin residue in non-fatty vegetables and fruits. The abamectin residues were extracted efficiently with acetonitrile assisting by ultrasound and then directly derivatized without requiring the clean-up step. Results showed that the developed method has good sensitivity and selectivity for determining the abamectin residues in vegetables and fruits. Limits of detection and limits of quantification were 0.0005 and 0.002 μg/g, respectively. The recoveries ranged from 83.2% to 123.7% with satisfactory precision (RSD > 16.7%) and bias (-16.8 ~ 23. 7%). The proposed method was successfully applied to analyze the real commercial samples. © 2010 Springer Science+Business Media, LLC.


Xie X.,Nanjing University | Yao F.,Zhejiang Agriculture And forestry University | Wu Y.,Nanjing University | Zhao L.,Shanghai Agricultural Extension and Service Center
International Journal of Environmental Analytical Chemistry | Year: 2012

A simple and sensitive method has been developed and validated for the determination of abamectin B1a (ABA B1a), emamectin B1a (EMA B1a) benzoate and ivermectin H2B1a (IVM H2B1a) in soils. The avermectins (AVMs) residues were extracted from soils with acetonitrile/water (9:1, v/v) and then were purified on C18 solid-phase extraction (SPE) cartridge. After being derivatised by N-methylimidazole (N-MIM) and trifluoroacetic anhydride (TFAA), the residues of three AVMs were analysed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The method was validated in terms of system suitability, linearity, selectivity, precision, recovery, specificity and stability. There was a good linear relationship (R2> 0.99) for three AVMs ranged from 0.01 to 5 μg mL-1. The LOD and LOQs of ABA B1a, EMA B1a benzoate and IVM H2B1a for standard solutions were 1.1-1.7 and 3.6-5.7 μg L-1 respectively. The accuracy of AVMs in soils was from 83.7 to 115.5% with precision less than or equal to 12.4%. Using the developed method, 9 soil samples with 9.3-12806.3 μg kg-1 of AVMs residues had been detected. © 2012 Copyright Taylor and Francis Group, LLC.


PubMed | East China University of Science and Technology and Shanghai Agricultural Extension and Service Center
Type: Journal Article | Journal: Environmental toxicology and pharmacology | Year: 2014

Decabromodiphenyl ether (BDE209) and tetrabromobisphenol A (TBBPA) are the main contaminants at e-waste recycling sites, and their potential toxicological effects have received extensive attention. However, the impact on soil culturable microbial population and enzyme activity of joint exposure to the two chemicals remains almost unknown. Therefore, indoor incubation tests were performed on control and contaminated soil samples to determine the eco-toxicological response in the joint presence of BDE209 and TBBPA for the first time. The results have demonstrated some notable toxic effects due to long-term exposure to either or both contaminants. The inhibition ratios of microbial populations increased with incubation time and increasing concentrations of BDE209 or TBBPA following certain dose-response relationships and time-effect trends. The response sensitivity sequence was fungi>bacteria>actinomycete. The influence of the two chemicals on soil enzymes reached peak values on day 7, and highly significant differences (P<0.01) were observed compared to the controls. Urease was more susceptive to the two chemicals than catalase and saccharase activities. Generally, the joint toxicity of both contaminants on soil microbes, catalase or saccharase activities indicated antagonistic effects, while, as for urease activity, addition role was dominant. Such observations have provided the useful information of potential ecological effects of brominated flame retardants contamination in the environment.

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