Time filter

Source Type

Zhang M.,Qingdao University | Zhang M.,Jining Medical University | Cai S.,Qingdao University | Ma J.,Fengxian Branch of Shanghai 6th Peoples Hospital
Gene | Year: 2015

The aim of the present study was to investigate whether soybean oligosaccharides (SO) protects heart function against myocardium ischemia reperfusion (MIR) injury. Hearts were 20. min global ischemia and 50. min reperfusion. Rats were fed for 30. days with saline (sham and MIR groups) or the SO (200 or 400. mg/kg body weight, daily). At the end of 30. days, the left main coronary artery was occluded for 30. min, followed by 24. h reperfusion, in anesthetized rats. Sham operated animals were subjected to the same surgical procedures, except that the suture under the left anterior descending coronary artery was not tied. Results showed that SO decreased malondialdehyde (MDA) level and increased antioxidant enzymes activities in the SO-treatment group. Pre-treated with SO it showed a significant recovery in cardiac contractile function, reduction in infarct size, and decrease in creatine kinase (CK), aspartate transaminase (AST) and lactate dehydrogenase (LDH) activities. Moreover, SO also significantly increased the expression of p-JAK2 and p-STAT3 proteins in rat heart. However, no significant change in JAK2 and STAT3 levels was observed. Activation of JAK2/STAT3 pathway showed a significant protective role in the SO-treatment group. Perhaps, the altered activation of the JAK2/STAT3 pathway in ischemic myocardium is one mechanism by which SO is cardioprotective. © 2014 Elsevier B.V.


Ma J.W.,Nanjing Medical University | Ma J.W.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
Molecular Biology Reports | Year: 2013

The aim of this study was to characterise the effects of ischemic preconditioning (IP) on heart function parameters (DST andDT), activities of serumcreatine kinase (CK), lactate dehydrogenase (LDH), and levels of serum nitric oxide (NO), malondialdehyde (MDA), and myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-Alpha (TNF-α) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium IR rats. Results showed that DST and DST values in IP group were markedly lower than those in IR group. Compared with IR group, IP significantly (p>0.01) decreased serum CK (0.83 ± 0.09 vs 1.36 ± 0.15), LDH (5613 ± 462 vs 7106 ± 492) activities and MDA (11.32 ± 1.05 vs 15.49 ± 1.26) level, increased the serum NO (86.39 ± 7.03 vs 53.77 ± 4.27) level in IR group. The IP induced a significant decreased in myocardium Caspase-3 mRNA (0.303 ± 0.021 vs 0.515 ± 0.022) gene expression (p>0.01) compared to IR model group. The IP induced a significant decreased in myocardium SOCS-1 (0.241 ± 0.031 vs 0.596 ± 0.036), SOCS-3 (0.258 ± 0.031 vs 0.713 ± 0.057), TNF-A (0.137 ± 0.011 vs 0.427 ± 0.035) and IL-6 (0.314 ± 0.021 vs 0.719 ± 0.064) mRNA gene expression (p>0.01) compared to IR model group. We conclude that IP is effective in the therapy of heart disease. These findings may have implications for the clinical development of preconditioning- based therapies for ischemic heart disease.© Springer Science+Business Media Dordrecht 2013.


Liu H.J.,Fengxian Branch of Shanghai 6th Peoples Hospital | Ma J.W.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
Molecular Biology Reports | Year: 2013

Prostaglandin E1 has been used clinically for improving heart diseases. In this study, we examined the effect of Prostaglandin E1 on blood lipid levels, heart protein and genes expression in coronary heart disease (CHD) rats. Female rats were fed either a control diet or hypercholesterolemic diet for 14 weeks. The feeding of a hypercholesterolemic diet (HCD) increased the serum TC, TG, and LDL-c levels, decreased the serum HDL-c, E2, P, FSH, LH and PRL levels in CHD rats. In addition, The feeding of a HCD diet markedly increased the content of serum TXA2, TXB2, and decreased the content of serum PGI2, and PGI2/TXA2, 6-Keto PGF1a. Furthermore, the feeding of a hypercholesterolemic diet markedly increased expression levels of myocardium Fas and Caspase-3 protein and mRNA levels, vascular endothelial growth factor and basic fibroblast growth factor mRNA, and decreased RyR2 mRNA in CHD rats. The feeding of Prostaglandin E1 for 14 weeks significantly reversed these abnormal biochemical indexes in rats. These findings suggest that Prostaglandin E1 play a obvious heart protective effect. The mechanisms may be related to restraining the excessive activation of Fas and Caspase-3 protein and modulating some gene expressions associated with CHD. © 2013 Springer Science+Business Media Dordrecht.


Ma J.,Nanjing Medical University | Ma J.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
International Journal of Biological Macromolecules | Year: 2013

In this study, we examined the effects of Prostaglandin E1 and tea polysaccharides (TP) on serum estrogen and FSH levels, myocardium sPLA2-V positive levels, and sPLA2-V protein expression in the rats fed on hypercholesterolemic diet. Hyperlipidemic rats were treated with Prostaglandin E1 and TP. Serum estrogen and FSH levels were significantly enhanced by Prostaglandin E1 and TP, whereas myocardium sPLA2-V positive rate and protein expression levels were decreased compared to the HCD group. Our results suggest that Prostaglandin E1 and TP exert strong heart-protective effects and therefore can be used to reduce the risk of heart disorders. © 2013 Elsevier B.V.


Qiao Z.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.,Tongji University | Ma J.,Fengxian Branch of Shanghai 6th Peoples Hospital | Liu H.,Fengxian Branch of Shanghai 6th Peoples Hospital
Molecules | Year: 2011

The present study was undertaken to evaluate the protection potential of ethanol extract of Salvia miltiorrhiza (SMEE) against oxidative injury in the ischemia-reperfusion (I/R) model of rats in vivo. Rats were divided into six groups of 10 rats each. Group I/R model and sham were fed with a standard rat chow, groups SMEE I and SMEE II were fed with a standard rat chow and 400 or 800 mg/kg b.w. ethanol extract for 12 days before the beginning of I/R studies. Positive control group was fed with a standard rat chow and salvianolic acid B (55 mg/kg b.w.) or tanshinone II-A (55 mg/kg b.w.) for 12 days before the beginning of I/R studies. To produce I/R, the left anterior descending artery (LAD) was occluded in anesthetized rats for 15 min, followed by 120 min reperfusion. Infarct sizes were found significantly decreased in SMEE-treated and positive control groups compared to I/R model group. Serum AST, LDH and CK-MB activities were significantly reduced and myocardium Na +-K + ATPase, Ca 2+-Mg 2+ ATPase activities and antioxidant enzyme activities (SOD, CAT, GSH-Px) were markedly increased in SMEE-treated and salvianolic acid B or tanshinone II-A positive control groups compared to the I/R model group. Pretreatment of S. miltiorrhiza ethanol extract and salvianolic acid B or tanshinone II-A dose-dependently reduced significantly myocardium MDA level, ROS and NOS activities and enhanced myocardium GSH level in I/R rats compared to I/R rats model. In conclusion, we clearly demonstrated that S. miltiorrhiza ethanol extract pretreatment can decrease oxidative injury in rats subjected to myocardial I/R. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.Y.,Tongji University | Huang J.H.,Fengxian Branch of Shanghai 6th Peoples Hospital | Ma J.W.,Fengxian Branch of Shanghai 6th Peoples Hospital | And 5 more authors.
Molecular Biology Reports | Year: 2014

The Bax, cyt-c and caspase-3 proteins play an important role in regulating the myocardial apoptosis. Although very little is known about the specific signal pathways modulated by Ginkgo biloba extract (GBE), it seems advisable to suppose that GBE-induced antiapoptotic effect might be attributed to the regulation of the expression of these proteins. Our aim was to investigate whether GBE could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. In the myocardium ischemia reperfusion (IR) rat model, treatment of GBE (400 mg/kg) significantly decreased the cardiomyocyte cell apoptosis and myocardium infarction. Immunohistochemical analysis showed that GBE significantly inhibited I/R-induced increase of myocardial Bax, caspase-3, and cyt-c proteins expression. Western blot analysis confirmed results of immunohistochemical analysis. It is most likely that multiple pathways are involved in IR-induced apoptosis in rat myocardium cells. Therefore, these results demonstrate that GBE exhibits significant protective effect against myocardial I/R injury in rat heart, which is related to down-regulate Bax, cyt-c and caspase-3. Bcl-2 overexpression might prevent IR-induced apoptosis by inhibiting cytochrome c release from the mitochondria and block activation of caspase-3. © 2013 Springer Science+Business Media Dordrecht.


Ge G.,Fengxian Branch of Shanghai 6th Peoples Hospital | Zhang Q.,Fengxian Branch of Shanghai 6th Peoples Hospital | Ma J.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.,Fengxian Branch of Shanghai 6th Peoples Hospital | And 3 more authors.
Gene | Year: 2014

Salvia miltiorrhiza has strong antioxidative activity. They may have a strong potential as cardioprotective agents in ischemic-reperfusion injury. Experiments were carried out in Sprague-Dawley rats with myocardium ischemia reperfusion (IR). Myocardial injuries during IR were determined by changes in electrocardiogram analysis of arrhythmias, antioxidant enzyme activities, AST, CK-MB, lactate dehydrogenase (LDH) levels, and myocyte apoptosis. Results showed that S. miltiorrhiza aqueous extract (SAME) pre-treatment significantly decreased the ST-segment (σST120) and myocardium MDA, AST, CK-MB, lactate dehydrogenase (LDH) levels, increased myocardium antioxidant enzyme activities, and inhibit myocardium cell apoptosis. Furthermore, the SAME pre-treatment significantly upregulated p-JAK2 and p-STAT3 protein expression, decreased myocardium TNF-α and IL-6 concentrations in IR rats. The levels of TNF-α and IL-6 were positively correlated with the changes in myocardium p-JAK2 and p-STAT3 protein expression levels in IR rats. It can be concluded that the SAME pre-treatment has anti-ischemic and anti-apoptosis activity in heart IR rats. SAME pre-treatment protects heart against IR injury, at least in part, through its stimulating effects on injury-induced deactivation of JAK2/STAT3 signaling pathway. © 2014 Elsevier B.V.


Jiangwei M.,Fengxian Branch of Shanghai 6th Peoples Hospital | Zengyong Q.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xia X.,Fengxian Branch of Shanghai 6th Peoples Hospital
Journal of Medicinal Plants Research | Year: 2011

Astragalus mongholicus is a Chinese traditional medicine. In this study, we sought to explore potential benefits in cardiovascular disorders associated with excess cholesterol and hyperlipidemia. We have investigated the effects of A. mongholicus extract as a dietary supplement on hyperlipidemia and oxidative stress in rats maintained on a high- cholesterol diet. Diets were supplemented with A. mongholicus extract at 0.4 and 0.8% for five weeks, while control animals received no supplement. A. mongholicus extract administration to hyperlipidemic rats resulted in a significant decline in serum levels of total cholesterol, triglycerides and low density lipoprotein-cholesterol, with an increase in serum high-density lipoprotein-cholesterol levels. Furthermore, A. mongholicus extract improved serum and heart antioxidant status as assessed by superoxide dismutase and glutathione peroxidase activities and reduced levels of lipid peroxidation. These results suggest that A. mongholicus extract consumption can improve lipid profiles, inhibit peroxidation, and increase the activity of antioxidant enzymes, and is thereby likely to reduce the risk of coronary heart disease associated with hyperlipidemia and oxidative stress. © 2011 Academic Journals.


Zengyong Q.,Fengxian Branch of Shanghai 6th Peoples Hospital | Jiangwei M.,Fengxian Branch of Shanghai 6th Peoples Hospital | Huajin L.,Fengxian Branch of Shanghai 6th Peoples Hospital
International Journal of Molecular Sciences | Year: 2011

We investigated the efficacy of Ligusticum wallichi aqueous extract (LWE) for myocardial protection against ischemia-reperfusion injury. Rats were fed for five weeks with either a control diet (sham and ischemia reperfusion (IR) model control groups) or a diet mixed with 0.2%, 0.4% or 0.6% Ligusticum wallichi extract. At the end of the five week period, hearts were excised and subjected to global ischemia for 30 min followed by reperfusion for 2 h. The hearts were compared for indices of oxidative stress and immunity activities. Administration of Ligusticum wallichi extract significantly decreased serum TNF-α, IL-6, IL-8, NO, MIP-1α, CRP and myocardium MDA levels, and serum CK, LDH and AST activities, and increased myocardium Na +-K +-ATPase, Ca 2+-Mg 2+-ATPase, NOS, SOD, CAT, GSH-Px and TAOC activities. The results indicate that Ligusticum wallichii extract treatment can enhance myocardial antioxidant status and improve the immunity profile in ischemic-reperfusion rats. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Fengxian Branch of Shanghai 6th Peoples Hospital
Type: Journal Article | Journal: Molecular biology reports | Year: 2014

The Bax, cyt-c and caspase-3 proteins play an important role in regulating the myocardial apoptosis. Although very little is known about the specific signal pathways modulated by Ginkgo biloba extract (GBE), it seems advisable to suppose that GBE-induced antiapoptotic effect might be attributed to the regulation of the expression of these proteins. Our aim was to investigate whether GBE could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. In the myocardium ischemia reperfusion (IR) rat model, treatment of GBE (400 mg/kg) significantly decreased the cardiomyocyte cell apoptosis and myocardium infarction. Immunohistochemical analysis showed that GBE significantly inhibited I/R-induced increase of myocardial Bax, caspase-3, and cyt-c proteins expression. Western blot analysis confirmed results of immunohistochemical analysis. It is most likely that multiple pathways are involved in IR-induced apoptosis in rat myocardium cells. Therefore, these results demonstrate that GBE exhibits significant protective effect against myocardial I/R injury in rat heart, which is related to down-regulate Bax, cyt-c and caspase-3. Bcl-2 overexpression might prevent IR-induced apoptosis by inhibiting cytochrome c release from the mitochondria and block activation of caspase-3.

Loading Fengxian Branch of Shanghai 6th Peoples Hospital collaborators
Loading Fengxian Branch of Shanghai 6th Peoples Hospital collaborators