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Shen M.Y.,Shandong Vocational Veterinary and Animal Science College | Sun Q.,Shandong Vocational Veterinary and Animal Science College | Zhang G.,Shandong Vocational Veterinary and Animal Science College | Yuan D.,Shandong Vocational Veterinary and Animal Science College | Li F.,Shandong Vocational Veterinary and Animal Science College
International Journal of Clinical and Experimental Medicine | Year: 2017

More and more studies on the antiviral activities of marine polysaccharides are catching more and more researcher’s attention in recent years. From couple years ago, the enteromorpha prolifera outbreak, this is becoming a great problem to the marine environment. The anti-porcine reproductive and respiratory syndrome virus (PRRSV) activity of polysaccharide enteromorpha prolifera (PEP) was studied in this research. Firstly, we confirmed that PEP inhibited the proliferation of PRRSV in vitro, and the inhibitive efficiency was dose dependent. The minimum dose to inhibit PRRSV proliferation was 1.56 mg/mL. Furthermore, the mechanism of anti-PRRSV was exploited in several ways, including viralcides assay, attachment inhibition assay, biosynthetic process inhibition assay. The results showed that PEP inhibited the attachment of PRRSV to Marc145 cells, completely suppressed the biosynthesis when it existed continuously. On the other hand, we also testified that the virucidal effect does not seem to be involved in the PEP antiviral activity detected. © 2017, E-Century Publishing Corporation. All rights reserved.

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