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Li Z.H.,Liaocheng University | Ma S.X.,Shandong Vocational Animal Science and Veterinary College | Kong L.Q.,Liaocheng University | Li D.C.,Liaocheng University
Zeitschrift fur Kristallographie - New Crystal Structures | Year: 2014

C28H34Cu2N6O10, monoclinic, P21/c (no. 14), a = 10.009(2) Å, b = 11.787(2) Å, c = 13.928(3) Å,β = 106.846(2)°, V = 1572.6 Å3, Z = 2, Rgt(F) = 0.0409, wRref(F2) = 0.1241, T = 298 K. © 2014 by Walter de Gruyter Berlin/Boston.


Li Z.H.,Liaocheng University | Ma S.X.,Shandong Vocational Animal Science and Veterinary College | Kong L.Q.,Liaocheng University | Li D.C.,Liaocheng University
Zeitschrift fur Kristallographie - New Crystal Structures | Year: 2014

C13H10CuN4O4, triclinic, P1 (no. 2), a = 6.653(1) Å, b = 8.439(2) Å, c = 11.931(2) Å, α = 107.585(3)°, β = 96.823(2)°, γ= 92.316(2)°, V = 632.0 Å3, Z = 2, Rgt(F) = 0.0320, wRref(F2) = 0.0838, T = 298 K. © 2014 by Walter de Gruyter Berlin/Boston.


Xia Y.,CAS Lanzhou Institute of Chemical Physics | Wang Z.,Shandong Vocational Animal Science and Veterinary College | Song Y.,CAS Lanzhou Institute of Chemical Physics | Song Y.,University of Chinese Academy of Sciences
Industrial Lubrication and Tribology | Year: 2014

Purpose - The aim of the present paper is that three long-chain hydroxyethyl alkylimidazolium hexa?uorophosphate ionic liquids (ILs) were synthesized and evaluated as lubricants for steel-steel contacts at room temperature and boundary lubrication conditions. Hydroxyethyl functional group and alkyl chain length effect on the physicochemical and fretting tribological behaviors of the ILs was comparatively investigated, as compared to traditional dialkylimidazolium ILs. Design/methodology/approach - The fretting friction and wear tests were carried out using an Optimol SRV-IV oscillating reciprocating friction and wear tester. The worn surface was observed and analyzed by scanning electron microscope and X-ray photoelectron spectroscopy. The electrochemical corrosion behavior of copper disks in selected imidazolium-based ionic liquids was studied using a CHI660B electrochemical workstation. Findings - This can be seen by comparing the results for the non-hydroxyl ILs and hydroxyl ILs that the latter exhibited the littler friction coefficient, the smaller wear volume, although the latter electrochemical corrosion behavior of copper disks is slightly higher than the former. The results also revealed that the ILs with a longer alkyl chain displayed larger viscosities, better anti-corrosion capacities, higher hydrophobic properties and more excellent friction-reducing and anti-wear performance than those with a shorter alkyl chain. Originality/value - This work might offer new knowledge in the design and application of new ILs as lubricants; it also confirms some in-depth physicochemical questions, e.g. the function mechanism, the correlations between structure and performance. Additionally, a proposed interaction model between the ILs and the friction substrate has been given. Copyright © 2014 Emerald Group Publishing Limited. All rights reserved.


Wang J.,Jiangsu Academy of Agricultural Sciences | Ge A.,Shandong Vocational Animal Science and Veterinary College | Xu M.,Jiangsu Academy of Agricultural Sciences | Wang Z.,Jiangsu Academy of Agricultural Sciences | And 7 more authors.
Virology Journal | Year: 2015

Abstract Background: Highly pathogenic avian influenza virus (AIV) subtype H5N1 remains a threat to poultry. Duck enteritis virus (DEV)-vectored vaccines expressing AIV H5N1 hemagglutinin (HA) may be viable AIV and DEV vaccine candidates. Methods: To facilitate the generation and further improvement of DEV-vectored HA(H5) vaccines, we first constructed an infectious clone of DEV Chinese vaccine strain C-KCE (DEVC-KCE). Then, we generated a DEV-vectored HA(H5) vaccine (DEV-H5(UL55)) based on the bacterial artificial chromosome (BAC) by inserting a synthesized HA(H5) expression cassette with a pMCMV IE promoter and a consensus HA sequence into the noncoding area between UL55 and LORF11. The immunogenicity and protective efficacy of the resulting recombinant vaccine against DEV and AIV H5N1 were evaluated in both ducks and chickens. Results: The successful construction of DEV BAC and DEV-H5(UL55) was verified by restriction fragment length polymorphism analysis. Recovered virus from the BAC or mutants showed similar growth kinetics to their parental viruses. The robust expression of HA in chicken embryo fibroblasts infected with the DEV-vectored vaccine was confirmed by indirect immunofluorescence and western blotting analyses. A single dose of 106 TCID50 DEV-vectored vaccine provided 100 % protection against duck viral enteritis in ducks, and the hemagglutination inhibition (HI) antibody titer of AIV H5N1 with a peak of 8.2 log2 was detected in 3-week-old layer chickens. In contrast, only very weak HI titers were observed in ducks immunized with 107 TCID50 DEV-vectored vaccine. A mortality rate of 60 % (6/10) was observed in 1-week-old specific pathogen free chickens inoculated with 106 TCID50 DEV-vectored vaccine. Conclusions: We demonstrate the following in this study. (i) The constructed BAC is a whole genome clone of DEVC-KCE. (ii) The insertion of an HA expression cassette sequence into the noncoding area between UL55 and LORF11 of DEVC-KCE affects neither the growth kinetics of the virus nor its protection against DEV. (iii) DEV-H5(UL55) can generate a strong humoral immune response in 3-week-old chickens, despite the virulence of this virus observed in 1-week-old chickens. (iv) DEV-H5(UL55) induces a weak HI titer in ducks. An increase in the HI titers induced by DEV-vectored HA(H5) will be required prior to its wide application. © 2015 Wang et al.


Gao L.,The First Affiliated Hospital of General Hospital of PLA | Wang X.,The First Affiliated Hospital of General Hospital of PLA | Ma J.,The First Affiliated Hospital of General Hospital of PLA | Hao D.,The First Affiliated Hospital of General Hospital of PLA | And 3 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2016

In the present studies locally injectable docetaxel nanocrystals loaded d-alpha tocopheryl polyethylene glycol 1000 succinate-modified Pluronic F127 (DOC-NCs-TPGS-PF127) thermo-sensitive hydrogels were prepared to reverse drug resistance of P-glycoprotein (P-gp)-overexpressing human liver cancer SMMC-7721 tumors. Firstly, DOC nanosuspensions with mean particle size of 196nm were prepared and dispersed into series of mixed solutions containing PF127 and TPGS of different ratios to obtain DOC-NCs-TPGS-PF127 hydrogels. DOC NCs, exhibiting a uniform distribution and very good physical stability during three sol-gel cycles in the hydrogel network, did not influence the gelation temperature. Swelling-dependent release pattern was found for DOC NCs from hydrogels and release profiles could be well fitted by the Peppas equation. MTT test showed that hydrogels containing 0% or 0.1% TPGS had no cytotoxicity against L929 fibroblasts. Both DOC solution and DOC-NCs-TPGS-PF127 hydrogels exhibited obvious cytotoxicity against sensitive SMMC-7721 cells. When resistant SMMC7721 cells were treated, DOC-NCs-TPGS-PF127 hydrogels showed significantly higher cytotoxicity compared with DOC solution and hydrogels containing no TPGS (DOC-NCs-PF127), with markedly lower IC50 and resistant index (RI). After intratumoral injection in SMMC-7721/RT tumor xenograft Balb/c mice model, DOC-NCs-TPGS-PF127 hydrogels exhibited about 5-fold increase and 1.8-fold increase in the inhibition rate of tumor growth compared with intravenous and intratumoral injection of DOC solution, respectively. It could be concluded that TPGS-modified PF127 thermo-sensitive hydrogel was an excellent locally injectable carrier to reverse P-gp overexpression associated multi-drug resistance. © 2016.

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