Shandong UniversityShandong

Linyi, China

Shandong UniversityShandong

Linyi, China
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Ni Y.,Shandong UniversityShandong | Le K.,Shandong UniversityShandong | Du W.,Shandong University | Fang W.,Shandong UniversityShandong | And 4 more authors.
Sensors and Actuators, B: Chemical | Year: 2017

The Sb2O5 modified SnO2 porous nanocomposites serving as NO2 gas sensing material have been successfully synthesized through a facile hydrothermal method followed by a calcination process. The porous Sb2O5/SnO2 nanocomposites display a dominant pore size of ca. 20 nm and specific surface area of 37.2 m2 g−1, which can provide large contact area for the chemical adsorption of NO2 molecules and abundant channels for the import and export of NO2 gas. Gas sensing tests demonstrated that the as-prepared porous Sb2O5/SnO2 nanocomposites (1 mol% Sb2O5) achieved superior sensing performances including high selectivity to NO2, low optimal operating temperature (ca. 100 °C), high response (800–5 ppm NO2), and short response and recovery times (20 s and 70 s to 5 ppm NO2, respectively). Comparing with pure SnO2 porous structure, the enhanced gas sensing performances of the porous Sb2O5/SnO2 nanocomposites are mainly ascribed to the p-n junctions generated from the hybrid of n-type SnO2 with p-type Sb2O5, which not only improved the response and selectivity to NO2 gas, but also reduced the operating temperature. © 2017 Elsevier B.V.


Zhang N.,Shandong UniversityShandong | Zhang N.,The Peoples Hospital of RizhaoShandong | Lv H.,The Peoples Hospital of RizhaoShandong | Shi B.-H.,The Peoples Hospital of RizhaoShandong | And 2 more authors.
Microbial Pathogenesis | Year: 2017

The aim of this study was to clarify the anti-inflammatory effects and its molecular mechanism of glycyrrhizin on LPS-stimulated human gingival fibroblasts (HGFs), which will be of benefit for periodontitis treatment. An MTT assay was performed to assess the effects of glycyrrhizin on cellular viability. The levels of IL-6 and IL-8 were measured by ELISA. The expression of iNOS, COX-2, NF-κB, and LXRα were detected by western blot analysis. The results showed that glycyrrhizin significantly inhibited LPS-induced IL-6 and IL-8 production, as well as COX-2 and iNOS expression. LPS-induced NF-κB activation in HGFs was also inhibited by treatment of glycyrrhizin. Furthermore, glycyrrhizin increased the expression of LXRα in a concentration-dependent manner. In addition, the inhibition of glycyrrhizin on IL-6 and IL-8 production was reversed by LXRα inhibitor GGPP. In conclusion, these results indicated that glycyrrhizin exhibited its anti-inflammatory effects in HGFs by activating LXRα. © 2017 Elsevier Ltd


Lin X.,Chinese PLA General Hospital | Lin C.-H.,Southern Taiwan University of Science and Technology | Zhao T.,Shandong UniversityShandong | Zuo D.,Chinese PLA General Hospital | And 3 more authors.
Chemico-Biological Interactions | Year: 2017

Heat stroke is characterized by hyperthermia, systemic inflammation, and multiple organ failure including arterial hypotension. This definition can be fulfilled by a rat model of heat stroke used in the present study. Anesthetized animals were exposed to heat exposure (43 °C for 70 min) and then returned to room temperature (26 °C) for recovery. One hour before heat exposure, an intraperitoneal dose of quercetin (30 mg/kg) or vehicle (normal saline 1 ml/kg) was administered to the experimental groups of rats. Additional injection was administered immediately after the onset of heat stroke. Immediately after the onset of heat stroke. Vehicle-treated rats displayed (i) hyperthermia; (ii) suppressed left ventricular function; (iii) decreased contents of cardiac total antioxiant capacity (e.g., superoxide dismutase, glutathione peroxidase, catalase); (iv) increased contents of cardiac oxidative capacity malondialdehyde and thiobarbituric acid reactive substances; (v) increased cardiac levels of pro-inflammatory cytokines tumor necrosis factor-α and interleukin-6; and (vi) decreased cardiac levels of an anti-inflammatory cytokine interleukin 10. Histopathologic and survival observation provided supportive evidence for biochemical analyses. These heat stroke reactions all can be significantly attenuated by quercetin therapy. Our data suggest that quercetin therapy might improve outcomes of heat stroke in rats by attenuating excessive hyperthermia as well as myocardial injury. The protective effects of quercetin could be attributed to anti-lipid peroxidative, anti-oxidant, and anti-inflammatory properties. © 2017 Elsevier B.V.


Wu Z.,Shandong University | Tan S.,Shandong University | Xu L.,Shandong University | Xu L.,Shandong UniversityShandong | And 5 more authors.
Antiviral Research | Year: 2017

Covalently closed circular DNA (cccDNA) in the hepatocytes nucleus is responsible for persistent infection of Hepatitis B virus (HBV). Current antiviral therapy drugs nucleos(t)ide analogs or interferon fail to eradicate HBV cccDNA. Genome editing technique provides an effective approach for HBV treatment through targeting viral cccDNA. Natronobacterium gregoryi Argonaute (NgAgo)-guide DNA (gDNA) system with powerful genome editing prompts us to explore its application in inhibiting HBV replication. Preliminary function verification indicated that NgAgo/EGFP-gDNA obviously inhibited EGFP expression. To further explore the potential role of NgAgo in restricting HBV replication, 10 of gDNAs targeting the critical region of viral genome were designed, only S-142, P-263 and P-2166 gDNAs led to significant inhibition on HBsAg, HBeAg and pregenomic RNA (pgRNA) level in Huh7 and HepG2 cells transfected with pcDNA-HBV1.1 plasmid. Similar results were also found in HBV infected HLCZ01 cells and Huh7-NTCP cells. However, we failed to detect any DNA editing in S-142, P-263 and P-2166 targeting region through T7E1 assay and Sanger sequencing. Remarkably, we found that NgAgo/P-2166 significantly accelerated the decay of viral pgRNA. Taken together, our results firstly demonstrate the potential of NgAgo/gDNA in inhibiting HBV replication through accelerating pgRNA degradation, but not DNA editing. © 2017 Elsevier B.V.


Chen X.C.,University of Hong Kong | Zeng Y.,University of Hong Kong | Au F.T.K.,University of Hong Kong | Jiang R.J.,University of Hong Kong | And 2 more authors.
Engineering Structures | Year: 2017

Prestressed concrete bridges with corrugated steel webs have emerged as one of the promising bridge forms. However, the presence of prestressing tendons and shear-deformable corrugated steel webs with negligible axial stiffness complicates the formation of plastic hinges under applied loading to failure. The full-range structural behaviour of these bridges and plastic hinge formation are therefore studied experimentally and numerically. The more localised flange plastic hinge caused by both web shear deformation and local flange bending interacts with the full-depth plastic hinge of such a bridge. Tests show that the full-range structural behaviour of the bridge is mainly governed by the localised flange plastic hinges. A formula is proposed to predict the equivalent interactive plastic hinge length, which helps to predict the full-range structural behaviour taking into account the presence of external prestressing tendons. Some design recommendations are also provided. © 2017 Elsevier Ltd


Wang Y.,University of Chinese Academy of Sciences | Zhang Y.,University of Chinese Academy of Sciences | Schauer J.J.,University of Wisconsin - Madison | de Foy B.,Saint Louis University | And 2 more authors.
Science of the Total Environment | Year: 2016

The Beijing government and its surrounding provinces implemented a series of measures to ensure haze-free skies during the 22nd Asia-Pacific Economic Cooperation (APEC) conference (November 10th–11th, 2014). These measures included restrictions on traffic, construction, and industrial activity. Twelve hour measurements of the concentration and composition of ambient fine particulate matter (PM2.5) were performed for 5 consecutive months near the APEC conference site before (September 11th–November 2nd, 2014), during (November 3rd–12th, 2014) and after (November 13th, 2014–January 31st, 2015). The measurements are used in a positive matrix factorization model to determine the contributions from seven sources of PM2.5: secondary aerosols, traffic exhaust, industrial emission, road dust, soil dust, biomass burning and residual oil combustion. The source apportionment results are integrated with backward trajectory analysis using Weather Research and Forecast (WRF) meteorological simulations, which determine the relative influence of new regulation and meteorology upon improved air quality during the APEC conference. Data show that controls are very effective, but meteorology must be taken into account to determine the actual influence of the controls on pollution reduction. The industry source control is the most effective for reducing concentrations, followed by secondary aerosol and biomass controls, while the least effective control is for the residual oil combustion source. The largest reductions in concentrations occur when air mass transport is from the west-northwest (Ulanqab). Secondary aerosol and traffic exhaust reductions are most significant for air mass transport from the north-northwest (Xilingele League) origin, and least significant for northeast transport (Chifeng via Tangshan conditions). The largest reductions of soil dust, biomass burning, and industrial source are distinctly seen for Ulanqab conditions and least distinct for Xilingele League. © 2016 Elsevier B.V.


Qiu G.-Z.,Shandong UniversityShandong | Tian W.,Linyi Oncosurgical HospitalShandong | Fu H.-T.,Shandong UniversityShandong | Li C.-P.,Eye Institute of XuzhouJiangsu | Liu B.,Tongji University
Biochemical and Biophysical Research Communications | Year: 2016

Microvascular dysfunction is an important characteristic of diabetic retinopathy. Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes. In this study, we investigated the role of lncRNA-MEG3 in diabetes-related microvascular dysfunction. We show that MEG3 expression level is significantly down-regulated in the retinas of STZ-induced diabetic mice, and endothelial cells upon high glucose and oxidative stress. MEG3 knockdown aggravates retinal vessel dysfunction in vivo, as shown by serious capillary degeneration, and increased microvascular leakage and inflammation. MEG3 knockdown also regulates retinal endothelial cell proliferation, migration, and tube formation in vitro. The role of MEG3 in endothelial cell function is mainly mediated by the activation of PI3k/Akt signaling. MEG3 up-regulation may serve as a therapeutic strategy for treating diabetes-related microvascular complications. © 2016 Elsevier Inc. All rights reserved.


Li G.,Shandong UniversityShandong | Kong L.,Shandong UniversityShandong | Zhou H.,Peoples Hospital of LiaochengShandong | Kang X.,Shandong UniversityShandong | And 2 more authors.
Sleep Medicine | Year: 2016

Objective To examine the relationship between prenatal maternal stress, resilience, and sleep quality, and to determine whether resilience plays a mediating role in the relationship between prenatal maternal stress and sleep quality among pregnant women. Methods Two hundred and thirty-one pregnant women in their second trimester participated in the study. They completed questionnaires, including: the Pittsburgh Sleep Quality Index (PSQI), the Pregnancy Stress Rating Scale (PSRS), and the 10-item Connor-Davidson Resilience Scale (CD-RISC-10). A structural equation model was used to analyze the relationships among prenatal maternal stress, resilience, and sleep quality, with resilience as a mediator. Results Prenatal maternal stress was negatively associated with sleep quality in pregnant women (p < 0.01), whereas resilience was positively associated with sleep quality (p < 0.01). Furthermore, resilience mediated the relationship between prenatal maternal stress and sleep quality, and the mediation effect ratio was 22.0% (p < 0.01). Conclusions The risk factor for disturbed sleep was pregnancy-specific stress; however, the protective factor for sleep quality was resilience. This finding could provide scientific evidence for the development of intervention strategies with which to improve sleep quality in pregnant women. © 2016 Elsevier B.V.


Yang Y.,Saarland University | Shrestha Y.R.,ETH Zurich | Li W.,Changsha University | Guo J.,Shandong UniversityShandong
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2016

In the k-Vertex-Disjoint Paths problem, we are given a graph G and k terminal pairs of vertices, and are asked whether there is a set of k vertex-disjoint paths linking these terminal pairs, respectively. In the k-Path problem, we are given a graph and are asked whether there is a path of length k. It is known that both problems are NP-hard even in split graphs, which are the graphs whose vertices can be partitioned into a clique and an independent set. We study kernelization for the two problems in split graphs. In particular, we derive a 4k vertex-kernel for the k-Vertex-Disjoint Paths problem and a 3/2k2+ ½k vertex-kernel for the k-Path problem. © Springer International Publishing Switzerland 2016.


Xu C.,Qingdao University | Yin X.,Shandong UniversityShandong | Sun X.,Qingdao University | Xing J.,Qingdao University | Sun Y.,Qingdao University
International Journal of Clinical and Experimental Medicine | Year: 2016

Baicalein is a medicinal herb and has various biological activities. Our present work aimed to evaluate the protective effect of baicalein on sepsis-associated encephalopathy (SAE) and further probe the potential mechanisms. SAE model was prepared using cecal ligation and puncture in mice. Animals were treated with saline or baicalein by doses of 10, 20 and 40 mg/kg for seven consecutive days. Neuronal function was assessed with the Open Field tests, Morris Water Maze task and Y Maze tests. The serum ammonia levels were measured after SAE. Besides, malondialdehyde (MDA) contents and the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and inducible nitric oxide synthase (iNOS) activities were also detected using respective commercial kits. Detection of NO production was conducted using Griess reagent. No significance was found in Open Field test. However, the cognitive deficits were remarkably improved in SAE-induced mice after baicalein treatment based on the results of Morris Water Maze and Y Maze tests. Meanwhile, baicalein didn’t alter the serum ammonia levels in SAE-induced mice. But baicalein significantly suppressed oxidative stress, the protein levels of iNOS and NO production while the protein expressions of BDNF was found to be markedly enhanced in mice with SAE after baicalein treatment. It was concluded that baicalein reversed cognitive deficits in a mouse model of SAE via suppressing oxidative stress and iNOS-mediated NO production and activating BDNF/TrkB signaling. © 2016, E-Century Publishing Corporation. All rights reserved.

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