Tumor Hospital of Shandong

Jinan, China

Tumor Hospital of Shandong

Jinan, China

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Chen G.-L.,Shandong University | Xiao Y.,Shandong University | Liu Y.-L.,Tumor Hospital of Shandong | Liu Z.-Y.,Shandong University
Acta Anatomica Sinica | Year: 2016

Objective To investigate whether human bone marrow mesenchymal stem cells ( HMSCs) are able to differentiate in vitro into corneal epithelial-like cells. Methods The primary human corneal keratocytes (HCFs) were obtained from the corneal stromal pieces by tissue culture, and the HMSCs were isolated by density gradient centrifugalization. Both the HMSCs and HCFs were identified according to their morphological characterizes, and the protein expression of CI)29 and vimentin were analyzed by immunocytochemistry. To induce cells differentiation, HMSCs were cultured by establishing epithelial culture microenvironment in a co-culture model. Cells were characterized by phase contrast microscopy observation. Using immunocytochemistry, we confirmed the protein presence of cornea-specific cytokeralin 12 ( CK12) in differentiated cells. The CK12 mRNA in differentiated cells was analyzed by reverse transcriptasepolymerase chain reaction ( RT-PCR). Results Most of the adherent HCFs and HMSCs were in spindle- shape , and perfectly aligned in a certain direction of the whirlpool-like shape while the cells were grown to near confluence. HMSCs were positively stained for CD29 and HCFs expressed vimentin. HMSCs changed the shape toward irregular polygon morphology after co-culture with HCFs over time, and the differentiating cells formed cell clusters some of which resembled epithelial-like cells. The expression of CK12 was positive for immunocytochemistry staining in differentiated cells, and further confirmed by RT-PCR. These results indicated that the differentiated cells acquired characteristics similar to those of corneal epithelial cells. Conclusion HMSCs isolated from human bone marrow are capable of differentiating into epithel ial-like cells and possess the phenotypical characteristics of corneal epithel ial cells.


Wang R.,Tianjin Medical University | Wang R.,Xinjiang Medical University | Tan Y.,Xinjiang Medical University | Wang X.,Xinjiang Medical University | And 7 more authors.
PLoS ONE | Year: 2014

Objectives: The objective of this study was to investigate the long-term outcomes and prognostic factors for nasopharyngeal carcinoma (NPC) in Han and Uyghur patients treated with intensity-modulated radiotherapy (IMRT) in the Xinjiang region of China.Materials and Methods: One hundred twenty-one Han and 60 Uyghur patients with newly diagnosed NPC without distant metastasis received IMRT at the Affiliated Tumor Hospital of Xinjiang Medical University between 2005 and 2008. The Kaplan-Meier method was used to estimate survival rates, and the log-rank test was used to evaluate differences in survival.Results: Comparing Han and Uyghur patients, the 5-year overall survival (OS), disease-free survival (DFS), local control (LC), regional control (RC), and distant metastasis-free survival (DMFS) rates were 81.9% vs 77.6% (P = 0.297), 72.1% vs 65.6% (P = 0.493), 88.3% vs 86.5% (P = 0.759), 95.0% vs 94.6% (P = 0.929), and 79.1% vs 75.2% (P = 0.613), respectively. Multivariate Cox proportional hazards regression identified the following independent prognostic factors in Han patients: N stage (P = 0.007) and age (P = 0.028) for OS, and age (P = 0.028) for DFS. OS differed significantly between Han and Uyghur patients >60 years old group (P = 0.036). Among Uyghur patients, the independent prognostic factors were age for OS (P = 0.033), as well as N stage (P = 0.037) and age (P = 0.021) for DFS. Additionally, Uyghur patients were less likely to experience mucositis and dermatitis than Han patients.Conclusion: Han and Uyghur patients with NPC had statistically significant differences in age, smoking history, and N staging. There was no significant difference in overall treatment outcomes with IMRT between these 2 ethnic populations in Xinjiang, China. © 2014 Wang et al.


PubMed | Tumor Hospital of Shandong and Xinjiang Medical University
Type: Journal Article | Journal: PloS one | Year: 2014

The objective of this study was to investigate the long-term outcomes and prognostic factors for nasopharyngeal carcinoma (NPC) in Han and Uyghur patients treated with intensity-modulated radiotherapy (IMRT) in the Xinjiang region of China.One hundred twenty-one Han and 60 Uyghur patients with newly diagnosed NPC without distant metastasis received IMRT at the Affiliated Tumor Hospital of Xinjiang Medical University between 2005 and 2008. The Kaplan-Meier method was used to estimate survival rates, and the log-rank test was used to evaluate differences in survival.Comparing Han and Uyghur patients, the 5-year overall survival (OS), disease-free survival (DFS), local control (LC), regional control (RC), and distant metastasis-free survival (DMFS) rates were 81.9% vs 77.6% (P=0.297), 72.1% vs 65.6% (P=0.493), 88.3% vs 86.5% (P=0.759), 95.0% vs 94.6% (P=0.929), and 79.1% vs 75.2% (P=0.613), respectively. Multivariate Cox proportional hazards regression identified the following independent prognostic factors in Han patients: N stage (P=0.007) and age (P=0.028) for OS, and age (P=0.028) for DFS. OS differed significantly between Han and Uyghur patients >60 years old group (P=0.036). Among Uyghur patients, the independent prognostic factors were age for OS (P=0.033), as well as N stage (P=0.037) and age (P=0.021) for DFS. Additionally, Uyghur patients were less likely to experience mucositis and dermatitis than Han patients.Han and Uyghur patients with NPC had statistically significant differences in age, smoking history, and N staging. There was no significant difference in overall treatment outcomes with IMRT between these 2 ethnic populations in Xinjiang, China.

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