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Bi S.,Shandong Sino Japanese Center for Collaborative Research of Carbon Nanomaterials | Bi S.,Qingdao University | Jia X.,Qingdao University of Science and Technology | Ye J.,Qingdao University of Science and Technology | Dong Y.,Qingdao University of Science and Technology
Biosensors and Bioelectronics | Year: 2015

Gold nanomaterials promise a wide range of potential applications in chemical and biological sensing, imaging, and catalysis. In this paper, we demonstrate a facile method for room-temperature synthesis of gold nanostars (AuNSs) with a size of ~50. nm via seeded growth. Significantly, the AuNSs are found to have high light-scattering properties, which are successfully used as labels for sensitive and selective detection of nucleic acids and proteins by using exonuclease III (Exo III) as a biocatalyst. For DNA detection, the binding of targets to the functionalized AuNS probes leads to the Exo III-stimulated cascade recycling amplification. As a result, a large amount of AuNSs are released from magnetic nanoparticles (MNPs) into solution, providing a greatly enhanced light-scattering signal for amplified sensing process. Moreover, a binding-induced DNA three-way junction (DNA TWJ) is introduced to thrombin detection, in which the binding of two aptamers to thrombin triggers assembly of the DNA motifs and initiates the subsequent DNA strand displacement reaction (SDR) and Exo III-assisted cascade recycling amplification. The detection limits of 89. fM and 5.6. pM are achieved for DNA and thrombin, respectively, which are comparable to or even exceed that of the reported isothermal amplification methods. It is noteworthy that based on the DNA TWJ strategy the sequences are independent on target proteins. Additionally, the employment of MNPs in the assays can not only simplify the operations but also improve the detection sensitivity. Therefore, the proposed amplified light-scattering assay with high sensitivity and selectivity, acceptable accuracy, and satisfactory versatility of analytes provides various applications in bioanalysis. © 2015 Elsevier B.V. Source


Lu L.,Shandong Sino Japanese Center for Collaborative Research of Carbon Nanomaterials | Lu L.,Qingdao University | Lu L.,Zibo Normal College | Zhang F.,Shandong Sino Japanese Center for Collaborative Research of Carbon Nanomaterials | And 7 more authors.
International Journal of Electrochemical Science | Year: 2015

A conductive carbon black-graphene (CCB-GR) modified glassy carbon electrode (CCB-GR/GCE) has been fabricated and used for determination of rutin. Transmission electron microscopy (TEM) results indicated that CCB-GR was successfully prepared. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) results showed that CCB-GR/GCE exhibited an excellent electrochemical performance. The sensing platform showed wide linear responses for rutin from 1×10-9 to 1×10-4 mol L-1 with a detection limit of 3.3×10-10 mol L-1 (S/N = 3). The CCB-GR/GCE showed excellent sensitive with good stability toward the determination of rutin content in tablet. © 2015 The Authors. Source


Wang Z.,Shandong Sino Japanese Center for Collaborative Research of Carbon Nanomaterials | Wang Z.,Qingdao University | Yu J.,Shandong Sino Japanese Center for Collaborative Research of Carbon Nanomaterials | Yu J.,Qingdao University | And 6 more authors.
Biosensors and Bioelectronics | Year: 2016

Carbon nanomaterials (CNMs) have attracted increasing attention due to their unique electrical, optical, thermal, mechanical and chemical properties. CNMs are extensively applied in electronic, optoelectronic, photovoltaic and sensing devices fields, especially in bioassay technology. These excellent properties significantly depend on not only the functional atomic structures of CNMs, but also the interactions with other materials, such as gold nanoparticles, SiO2, chitosan, etc. This review systematically summarizes applications of CNMs in electrochemical aptasensors (ECASs). Firstly, definition and development of ECASs are introduced. Secondly, different ways of ECASs about working principles, classification and construction of CNMs are illustrated. Thirdly, the applications of different CNMs used in ECASs are discussed. In this review, different types of CNMs are involved such as carbon nanotubes, graphene, graphene oxide, etc. Besides, the newly emerging CNMs and CNMs-based composites are also discoursed. Finally, we demonstrate the future prospects of CNMs-based ECASs, and some suggestions about the near future development of CNMs-based ECASs are highlighted. © 2015 Elsevier B.V. Source

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