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Wang Y.G.,CAS Institute of Genetics and Developmental Biology | Wang S.P.,Shandong Shengfeng Seeds Co. | Li H.J.,Shandong Shengfeng Seeds Co.
Genetics and Molecular Research | Year: 2015

In this study, the full-length cDNA encoding allene oxide cyclase (AhAOC) was isolated from peanut (Arachis hypogaea L.). The deduced amino acid sequence of AhAOC showed high homology with other plant AOCs. The transcript of AhAOC was found to be abundantly expressed in roots. Expression analysis demonstrated that AhAOC was induced by abscisic acid, methyl-jasmonic acid, salicylic acid, salinity, polyethylene glycol, and cold stresses, particularly by high salinity. Overexpression of AhAOC in rice increased root elongation and plant height compared with expression in control plants and conferred tolerance against salinity. Thus, the AhAOC gene may play an important role in increasing the expression of transcription factors (MYB2 and OsONAC045) and functional genes (DREB1F and LEA3) in transgenic rice under salt stress as well as improve stress tolerance through the accumulation of compatible solutes (proline and soluble sugar). The AhAOC gene is a potential resource for enhancing salt tolerance in crop species. © FUNPEC-RP. Source


Wang Y.-G.,CAS Institute of Genetics and Developmental Biology | Wang S.-P.,Shandong Shengfeng Seeds Co. | Li H.-J.,Shandong Shengfeng Seeds Co. | Xin Q.-G.,Yantai Academy of Agricultural science
Acta Physiologiae Plantarum | Year: 2014

Allene oxide synthase (AOS, EC 4.2.1.92) is the first specific jasmonate biosynthetic pathway gene. In this study, a full-length cDNA of AOS gene was cloned from common wheat nannong 9918. The gene contains an open reading frame (1,446 bp) encoding 418 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of TaAOS was highly homologous to AOSs from other plant species. The transcript of TaAOS was found to be abundantly expressed in the flag leaves, and was up-regulated by the inoculation of B. Graminis (DC.) E.O. Speer f. sp. Tritici (Bgt) in wheat leaves. In addition, it was also induced by high concentration of NaCl and ZnCl2. When TaAOS was overexpressed in tobacco leaves via Agrobacterium tumefaciens infection, the transgenic tobacco plants displayed stronger tolerance to ZnCl2 stress compared to transgenic GFP plants. Taken together, the above facts demonstrated that TaAOS may play a role in response to diverse stresses in plants. © 2014 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Source


Wang Y.,CAS Institute of Genetics and Developmental Biology | Wang S.,Shandong Shengfeng Seeds Co. | Li H.,Shandong Shengfeng Seeds Co. | Xin Q.,Yantai Academy of Agricultural science
Plant Cell, Tissue and Organ Culture | Year: 2015

A common wheat cDNA encoding a prolyl aminopeptidase (PAP, EC 3.4.11.5) was cloned by RT-PCR method and has been named as TaPAP1. The cDNA of TaPAP1 is 1,173 bp in length and encodes a predicted protein of 391 amino acids with a molecular mass and isoelectric point were 43.9 kDa and 7.94, respectively. Alignment of protein sequence indicated that TaPAP1 was evolutionarily conserved among other plants. Real-time PCR analysis revealed that TaPAP1 was predominantly expressed in seedling roots and up-regulated by various abiotic stresses, such as salt, drought, cold, copper and zinc. Compared to wild type plants, zinc-stressed TaPAP1 transgenic Arabidopsis displayed higher survival rate, the fresh weight, photosynthetic efficiency, proline levels, and PAP activity. Our results suggested that TaPAP1 controlled plant tolerance to zinc stress by means of improving the proline levels and PAP enzyme activity. Subcellular localization analysis showed that protein TaPAP1 is localized mainly in the cytoplasm. In conclusion, a novel cDNA sequence encoding wheat PAP gene was successfully obtained and the results showed that TaPAP1 is involved in the plant response to zinc stress, indicating a potential use in the transgenic breeding to improve heavy metal resistance in crop species. © 2015, Springer Science+Business Media Dordrecht. Source

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