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Chenghu L.,Shandong Quality Inspection Center for Medical Devices
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2010

To improve our comprehensions to complement activation by biomaterial and lay the foundation for biosafety evaluation of solid biomaterials together with the corresponding blood contacting medical devices. Analyzed new requirements of current standards on complement activation by solid biomaterial as well as the mechanism of complement activation by solid biomaterial and how to select the related standards for inspection. The new edition of international standards has enhanced types of blood contacting medical devices which are appropriate to complement activation test. It is badly in need of establishing the corresponding industry standards to regulate these requirements, since there have no uniform and admissive methods for inspection of complement activation by solid biomaterial.


Wang Y.,Shandong University | Wang C.-M.,Jining Medical University | Jiang Z.-Z.,Chinese Frontier Defence Armed Police General Hospital | Yu X.-J.,Shandong University | And 9 more authors.
Oncology Letters | Year: 2015

MicroRNAs (miRs) are short, non-coding RNAs with post-transcriptional regulatory functions. Previous studies have demonstrated that miR-34c is involved in diverse biological processes, including carcinogenesis. The aim of the present study was to investigate the role of miR-34c and its target genes in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Expression levels of miR-34c and its predicted target genes were measured. The target genes were validated by a luciferase assay. The effects of miR-34c restoration were evaluated by the detection of HBV antigens, cell proliferation and apoptosis in vitro, in addition to the tumor growth in vivo. The data demonstrated that miR-34c was downregulated in HBV-associated HCC clinical tissues and HCC cell lines compared with their corresponding controls. transforming growth factor-β-induced factor homeobox 2 (TGIF2), a transcription factor repressing transforming growth factor-β (TGFβ) signaling, was observed to be upregulated and was identified as a target gene of miR-34c. The restoration of miR-34c in HepG2.2.15 cells suppressed TGIF2 expression, HBV replication and viral antigen synthesis; inhibited cell proliferation; and induced apoptosis. miR-34c also inhibited tumor growth in a mouse model. The present study indicates that miR-34c may act as a tumor suppressor by targeting TGIF2 during HBV-associated hepatocellular carcinogenesis. miR-34c and TGIF2 may represent key regulatory factors, diagnostic markers and therapeutic targets for the prevention and treatment of HBV-associated HCC. © Spandidos Publications. All rights reserved.


Liang W.,Nanjing University | Han Q.,CAS Institute of Genetics and Developmental Biology | Han Q.,University of Chinese Academy of Sciences | Jin W.,Nanjing University | And 7 more authors.
Biomaterials | Year: 2010

Spinal cord crushed injury is clinically common. Promoting targeted neural regeneration at the crushed site of spinal cord could be important for the repair. It has been demonstrated in our previous work that native human BDNF fused with a collagen-binding domain (CBD-BDNF) can bind to collagen specifically to exert the neurotrophic effect on promoting axonal regeneration. After injury, collagen is highly accumulated at the injury site. We thus speculate that CBD-BDNF will bind to the extracellular matrix collagen and concentrate at the injury site to improve the therapy. Using the rat spinal cord crushed injury model, we have found that CBD-BDNF by one-time intrathecally injection could be retained and concentrated at the injury site for a longer time than native BDNF without collagen-binding domain. CBD-BDNF could promote better neural regeneration and locomotion recovery. © 2010 Elsevier Ltd.


Han Q.Q.,CAS Institute of Genetics and Developmental Biology | Han Q.Q.,University of Chinese Academy of Sciences | Jin W.,Nanjing University | Xiao Z.F.,CAS Institute of Genetics and Developmental Biology | And 7 more authors.
Biomaterials | Year: 2011

Brain derived neurotrophic factor (BDNF) has been shown to ameliorate recovery after intracerebral hemorrhage (ICH). The injured brain tissue after ICH is surrounded by hematoma formed from hemorrhage. Fibrin is abundant in hematoma, which could be a binding target for BDNF. In this work, we have fused a fibrin-binding domain (FBD) to BDNF (FBD-BDNF), and results demonstrate that FBD-BDNF has specific binding ability to fibrin and is retained in hematoma. Using the rat ICH model induced by bacterial collagenase, injected FBD-BDNF has been concentrated and retained at the hematoma. FBD has facilitated BDNF to exert targeting neuroprotective effect to the injured brain tissue around the hematoma after ICH. FBD-BDNF has significantly reduced the hemotoma volume, reduced tissue loss, promoted neural regeneration, and improved the rat behavioral performance. © 2011 Elsevier Ltd.


Yue M.,Shandong University | Zhang M.,Shandong University | Liu B.,Shandong Quality Inspection Center for Medical Devices | Xu X.,Shandong University | And 3 more authors.
Chinese Journal of Chemical Engineering | Year: 2013

Modified peanut shell (MPS) was prepared by amination reaction with peanut shell (PS) as the starting material. The sorption of Cr(VI) oxyanions on MPS in static and column tests were investigated. In addition, the sorption isotherm and kinetic models were applied to confirm the sorption capacity and the sorption mechanisms. BET surface area analysis showed the physicochemical characteristics of the samples. The results of zeta potential, Fourier transform infrared (FT-IR) and Raman spectra analysis illustrated that chemical adsorption and ion exchange are the potential sorption mechanism. The static sorption test showed that the maximum sorption capacity (qmax) of MPS for Cr(VI) increased with temperature, which indicated that the Cr(VI) sorption process was endothermic. The saturated sorption capacity of Cr(VI) in the column sorption test was 138.34 mg·g-1, which accounted for 93.9% of the qmax at 25°C. The regeneration capacity of MPS was evaluated using HCl solution as an eluent. The high regeneration efficiency (82.6%) validated the dominance of the ion exchange mechanism in the Cr(VI) sorption process with Cl- ions displacing Cr(VI) oxyanion on MPS. The Langmuir isotherm model showed a higher correlation coefficient than the other adsorption isotherm models. And in the kinetic study, a pseudo-second-order model fit the data best. © 2013 Chemical Industry and Engineering Society of China (CIESC) and Chemical Industry Press (CIP).

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