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Liu H.,Shandong University | Li Y.,Shandong University | Chen Y.,Shandong University | Wu W.,Shandong University | Zou D.,Shandong Provincial Qianfoshan Hospital
European Spine Journal | Year: 2013

Objective: Factors impacting surgical options and outcomes in patients with cervical ossification of the posterior longitudinal ligament (OPLL) were explored. Methods: A retrospective analysis was conducted of 127 eligible cervical OPLL patients (61 males, 66 females) aged 41-70 years (mean 55.2 years) selected from 152 total OPLL patients treated from 2002 to 2006, with 5-10-year (mean 6.8 years) follow-up. Patients underwent anterior subtotal corpectomy with ossification ligament resection (anterior surgery, n = 68) or posterior cervical double-door laminoplasty (posterior surgery, n = 59). Radiographic assessments of cervical curvature, T2-weighted MRI (MRIT2) signal, and OPLL occupying ratio were correlated with surgical strategy before surgery and at 1, 5 weeks, and 5 years. Results: Lordosis increased following anterior surgery, though kyphosis improved by 10.3 %. The canal stenosis occupying ratio was >50 %, and short-term improvement following anterior surgery was significantly higher than posterior surgery (P > 0.0001). Superior neurological function was observed in patients with unchanged versus high spinal MRIT2 signals (P = 0.0434). No significant differences were observed in short-term outcomes between anterior and posterior surgeries in high spinal MRIT2 signal patients, but anterior surgery produced significantly better long-term outcomes at 1 week (P = 0.7564) and 1 year (P = 0.0071). Complications occurred in five anterior and three posterior surgeries. Conclusion: Preoperative assessment of cervical curvature, MRIT2 signal, and occupying ratio can be used to guide clinical surgical approach selection to potentially produce better long-term outcomes in patients with OPLL. © 2013 Springer-Verlag Berlin Heidelberg.

Yao Z.-H.,Shandong University | Tian G.-Y.,Jinan Childrens Hospital | Wan Y.-Y.,Shandong University | Kang Y.-M.,Shandong Provincial Qianfoshan Hospital | And 3 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2013

Purpose: Nutritional status has been associated with long-time outcomes in cancer patients. We investigated whether the prognostic nutritional index (PNI), an indicator of nutritional status, affects overall survival in patients with malignant pleural mesothelioma (MPM). Methods: We enrolled 121 patients with histologically confirmed MPM, who had successfully undergone biopsy by medical thoracoscopy in this study. Demographic, clinical and laboratory data were collected retrospectively. The PNI was calculated as 10× serum albumin value (g/dl) + 0.005 × total lymphocyte count (per mm3) in peripheral blood. Univariate and multivariate analyses were used to identify prognostic factors. Results: Mean pretreatment PNI was 44.6. PNI was significantly associated with age (P = 0.031), smoking habits (P = 0.039) and weight loss (P = 0.029). Survival analysis showed PNI to be an independent prognostic factor in MPM. Patients with lower PNIs (PNI < 44.6) had greater risk of death than those with higher PNIs (PNI ≥ 44.6; hazard ratio: 2.290; 95 % confidence interval: 1.415-3.706; P = 0.001). These analyses were adjusted for patient age, gender, smoking habits, dyspnea, chest pain, weight loss, primary site of tumor, histology, platinum-based systemic chemotherapy, hospital and stage. Conclusions: Pretreatment PNI is a novel independent prognostic factor in MPM. © 2013 Springer-Verlag Berlin Heidelberg.

Xia L.J.,Shandong Provincial Qianfoshan Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2012

To investigate the effects of n-3 polyunsaturated fatty acids(n-3PUFA) on human colorectal cancer cell line HT-29 and associated mechanism. The effects of docosahexaenoic acid (DHA) on the proliferation and apoptosis on HT-29 human colorectal cancer cells were evaluated by MTT assay, cell morphology (Hoechst33258 dyeing), DNA gel electrophoresis, and flow cytometry. The content of n-6PUFA and n-3PUFA of the treated cells and the ratio of n-6/n-3PUFA were analyzed by chromatography. DHA effectively inhibited HT-29 cell proliferation in a dose- and time-dependent manner. The proliferative inhibition rates of HT-29 cells treated with 10, 20, 40, and 80 mg/L DHA for 24 hours were 16.8%, 24.7%, 50.0%, and 60.1%, respectively, while the inhibition rates were 50.0%, 69.9%, and 77.0% respectively when HT-29 cells were treated with 40 mg/L DHA for 24, 48, and 72 hours. The typical apoptotic morphologic changes of HT-29 cells could be observed, including chromatin margination, nuclear condensation and apoptotic bodies. Gel electrophoresis of DNA degradation displayed typical DNA ladder fragments. HT-29 cells treated with DHA were arrested in G1 phase and the proportion of HT-29 cells in Gl phase increased compared with that of the control group (72.1% vs. 51.3%) while the proportion of the cells in S phase decreased significantly (19.9% vs. 38.9%). The content of n-6PUFA decreased, n-3PUFA content increased and the ratio of n-6/n-3PUFA lowered significantly in colorectal cancer cells treated with DHA (P<0.01). n-3PUFA can inhibit the growth of human colorectal cancer cells via inhibition of the proliferation and induction of apoptosis. These effects may be associated with decrease in n-6/n-3PUFA ratio.

Wu L.,Shandong University | Wang H.,Shandong Provincial Qianfoshan Hospital | Lu X.,Shandong Academy of Sciences | Ma Q.,Shandong University | And 2 more authors.
Pharmaceutical Research | Year: 2013

Purpose: To develop PEGylated multi-walled carbon nanotubes as a sustained release drug delivery system. Methods: Oxaliplatin was incorporated into inner cavity of PEGylated multi-walled carbon nanotubes (MWCNT-PEG) using nano-extraction. Oxaliplatin release rates from MWCNT-PEG-Oxaliplatin were investigated using dialysis tubing. Cytotoxicity of oxaliplatin, MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin were evaluated in HT29 cell by MTT assay, Pt-DNA adducts formation, γ-H2AX formation and cell apoptosis assay. Results: Loading of oxaliplatin into MWCNT-PEG was ~43.6%. Sustained release occurred to MWCNT-PEG-Oxaliplatin, with only 34% of oxaliplatin released into medium within 6 h. In MTT assay, MWCNT-PEG-Oxaliplatin showed slightly decreased cytotoxic effect when cell viability was assessed at 12 and 24 h. A drastic increase of cytotoxicity was found when cell viability was assessed at 48 and 96 h. Pt-DNA adducts formation, γ-H2AX formation and cell apoptosis assay results showed the same trend as the MTT assay, suggesting sustained-release for MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin formulations. Conclusions: PEGylated multi-walled carbon nanotubes can be used as sustained release drug delivery system, thus remarkably improving cytotoxicity of oxaliplatin on HT-29 cells. © 2012 Springer Science+Business Media, LLC.

Li Y.,Shandong University | Ma Y.,Shandong Pharmaceutical Industry Research Institute | Zhang L.,Shandong University | Guo F.,Shandong Pharmaceutical Industry Research Institute | And 3 more authors.
PLoS ONE | Year: 2012

Riccardin D, a macrocyclic bisbibenzyl isolated from Chinese liverwort Dumortiera hirsute, has been proved to have inhibitory effect on biofilms formation of Candida albicans in in vitro study. Our present study aims to investigate the in vivo effect and mechanisms of riccardin D against C. albicans biofilms when used alone or in combination with clinical using antifungal agent fluconazole. XTT reduction assay revealed riccardin D had both prophylactic and therapeutic effect against C. albicans biofilms formation in a dose-dependent manner when using a central venous catheter related infective animal model. Scanning electron microscope and laser confocal scanning microscope showed that the morphology of biofilms was altered remarkably after riccardin D treatment, especially hypha growth inhibition. To uncover the underlying molecular mechanisms, quantitative real-time RT-PCR was performed to observe the variation of related genes. The downregulation of hypha-specific genes such as ALS1, ALS3, ECE1, EFG1, HWP1 and CDC35 following riccardin D treatment suggested riccardin D inhibited the Ras-cAMP-Efg pathway to retard the hypha formation, then leading to the defect of biofilms maturation. Moreover, riccardin D displayed an increased antifungal activity when administered in combination with fluconazole. Our study provides a potential clinical application to eliminate the biofilms of relevant pathogens. © 2012 Li et al.

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