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Guo D.,Shandong Provincial Key Laboratory of Integrated Traditional Chinese | Guo D.,Therapy of Ocular Diseases in Universities of Shandong | Guo D.,Shandong University of Traditional Chinese Medicine | Gu P.,Thermo Fisher Scientific | And 6 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

Uveitis is a severe autoimmune eye disease that can cause intraocular inflammation even lead to severe vision loss, and the occurrence of uveitis can be closely associated with abnormal expression of proteins. However, the abnormally expressed proteins involved in uveitis are not well identified. Using liquid chromatography-tandem mass spectrometry technique, we examined the alterations in proteomic expression profiling in rat plasma specimens related to experimental autoimmune uveitis (EAU) versus normal samples. In addition, the experimental verification was further performed using enzyme-linked immunosorbent assay (ELISA) for abnormally expressed proteins in EAU rat plasma. The results indicate that 62 proteins were upregulated and 106 proteins were downregulated in plasma from EAU rats compared with those in saline-treated samples. In the meantime, we observed that the plasma level of complement component 3 in EAU rats was upregulated versus saline-treated rats (from 92.32. μg/mL to 168.92. μg/mL), whereas the level of interleukin-1 receptor accessory protein was downregulated (from 1120.97. pg/mL to 798.39. pg/mL), and these results were highly in agreement with those of mass spectrometry determination. Taken together, our results indicate that liquid chromatography-tandem mass spectrometry analysis possesses a good resolution for peptides in plasma, and the findings will provide the baseline plasma dataset for EAU rats and the relevant information can contribute to future studies on the understanding the mechanism of uveitis. © 2014 Elsevier B.V.

Wu Q.,Shandong University of Traditional Chinese Medicine | Guo D.,Shandong Provincial Key Laboratory of Integrated Traditional Chinese | Guo D.,Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong | Guo D.,Shandong University of Traditional Chinese Medicine | And 6 more authors.
Photochemistry and Photobiology | Year: 2014

Currently, titanium dioxide nanoparticles (TiO2NPs) have been widely used in various applications including cosmetics, food additives and biomedicine. However, there are few reports available using TiO2NPs to treat ocular diseases. Posterior capsular opacification (PCO) is the most frequent complication after cataract surgery, which is induced by the proliferation and migration of lens epithelial cells. Thus, inhibiting the proliferation of lens epithelial cells will efficiently reduce the occurrence of PCO. In this study, we investigated the effects of TiO2NPs on HLE B-3 cells with or without ultraviolet B (UVB) irradiation in vitro. We found that TiO2NPs can inhibit HLE B-3 cell growth, cause the elevation of intracellular [Ca2+], produce excessive reactive oxygen species (ROS), further reduce Ca2+-ATPase activity and decrease the expression of plasma membrane calcium ATPase 1 (PMCA1), finally disrupt the intracellular calcium homeostasis and induce cell damage. Importantly, UVB irradiation can apparently enhance these effects on HLE B-3 cells in the presence of TiO2NPs. Taken together, the generation of excessive ROS and the disruption of intracellular calcium homeostasis may be both involved in TiO2nanoparticle-induced HLE B-3 cell damage under UVB irradiation. © 2014 The American Society of Photobiology.

Sha F.,Shandong University of Traditional Chinese Medicine | Ye X.,Shandong Provincial Key Laboratory of Integrated Traditional Chinese | Ye X.,Therapy of Ocular Diseases in Universities of Shandong | Ye X.,Shandong University of Traditional Chinese Medicine | And 21 more authors.
Neuroscience | Year: 2015

Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter of the retina and affects myopic development. Electroacupuncture (EA) is widely utilized to treat myopia in clinical settings. However, there are few reports on whether EA affects the level of retinal GABA during myopic development. To study this issue, in the present study, we explored the changes of retinal GABA content and the expression of its receptor subtypes, and the effects of EA stimulation on them in a guinea pig model with lens-induced myopia (LIM). Our results showed that the content of GABA and the expression of GABAA and GABAC receptors of retina were up-regulated during the development of myopia, and this up-regulation was inhibited by applying EA to Hegu (LI4) and Taiyang (EX-HN5) acupoints. Moreover, these effects of EA show a positional specificity. While applying EA at a sham acupoint, no apparent change of myopic retinal GABA and its receptor subtypes was observed. Taken together, our findings suggest that LIM is effective to up-regulate the level of retinal GABA, GABAA and GABAC receptors in guinea pigs and the effect may be inhibited by EA stimulation at LI4 and EX-HN5 acupoints. © 2014 IBRO.

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