Shandong Provincial Key Laboratory of Crop Genetic Improvement

Jinan, China

Shandong Provincial Key Laboratory of Crop Genetic Improvement

Jinan, China

Time filter

Source Type

Kong L.,Shandong Academy of Agricultural Sciences | Si J.,Shandong Academy of Agricultural Sciences | Sun M.,Shandong Academy of Agricultural Sciences | Feng B.,Shandong Academy of Agricultural Sciences | And 5 more authors.
Journal of Agronomy and Crop Science | Year: 2013

Root activity plays a dominant role in grain filling in cereal crops. However, the importance of deep roots for regulating post-anthesis leaf senescence is not clearly understood in wheat (Triticum aestivum L.). In this study, we used 32P tracing to estimate the difference in wheat root activity at soil depths of 30 and 70 cm and the root restriction method to investigate the effects of vertical distribution of deep roots on leaf senescence, with non-restricted plants as controls. Recovery of radioactive 32P indicated that deep roots had significantly higher activity than upper roots in wheat. Root restriction at a soil depth of 50 cm caused significant decreases in the activities of superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6) and ascorbate peroxidase (EC 1.11.1.11) at 16 days after anthesis and thereafter resulting in an increase in malondialdehyde. As a result, chlorophyll levels and net photosynthesis decreased. Ultimately, the root-restricted wheat produced a significantly lower grain yield than the non-restricted controls. These data suggest that deep roots are pivotal for regulating plant senescence, duration of grain filling, and yield formation. © 2012 Blackwell Verlag GmbH.


Li Y.,Shandong Academy of Agricultural Sciences | Li Y.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Fang F.,Shandong Academy of Agricultural Sciences | Guo F.,Shandong Academy of Agricultural Sciences | And 4 more authors.
Functional Plant Biology | Year: 2015

One of salt-induced calcium-dependent protein kinases (CDPKs) gene was isolated from Arachis hypogeae L. by RACE method. The cDNA full length was 2241bp deposited in GenBank (number KF437909), designated as AhCDPK. The coding region sequence of AhCDPK was 1629bp and encoded a protein of 542 amino acids. The molecular weight and the theoretical isoelectric point of AhCDPK was 60.96kDa and 5.61 respectively. Amino acid sequence analysis indicated that AhCDPK has highest similarity and homology with Glycine max L. In addition, the AhCDPK amino acids were predicted to encode a hydrophilic protein which localised in the endoplasmic reticulum. AhCDPK seemed to transcript in all peanut organs, and had the highest expression in seeds. The expression of AhCDPK could be strongly induced by both Ca2+ and NaCl. When exposed to salt stress, overexpressing AhCDPK in tobacco could alleviate PSII photoinhibition by improving physiological states, such as reducing the accumulation of reactive oxygen species (ROS), improving the activity of antioxidant defence system enzymes and improving the accumulation of osmotic regulation substance. These results showed that AhCDPK has the same functions as that of G. max, and it could play an important role for peanut to resist salt stress. © CSIRO 2015.


Mu C.H.,Shandong Academy of Agricultural Sciences | Mu C.H.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Yang Y.,Shandong Academy of Agricultural Sciences | Zhang F.J.,Shandong Academy of Agricultural Sciences | And 6 more authors.
Crop Breeding and Applied Biotechnology | Year: 2016

Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest. © 2016, Brazilian Society of Plant Breeding. All Rights Reserved.


Peng Z.,Shandong Academy of Agricultural Sciences | Peng Z.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Li L.,Shandong Normal University | Yang L.,Shandong Academy of Agricultural Sciences | And 8 more authors.
PLoS ONE | Year: 2013

Diacylglycerol acyltransferase (DGAT) is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the peanut cultivar 'Luhua 14' using a homologous gene sequence method and rapid amplification of cDNA ends. To understand the role of AhDGAT2 in triacylglycerol biosynthesis, two AhDGAT2 nucleotide sequences that differed by three amino acids were expressed as glutathione S-transferase (GST) fusion proteins in Escherichia coli Rosetta (DE3). Following IPTG induction, the isozymes (AhDGAT2a and AhDGAT2b) were expressed as 64.5 kDa GST fusion proteins. Both AhDGAT2a and AhDGAT2b occurred in the host cell cytoplasm and inclusion bodies, with larger amounts in the inclusion bodies. Overexpression of AhDGATs depressed the host cell growth rates relative to non-transformed cells, but cells harboring empty-vector, AhDGAT2a-GST, or AhDGAT2b-GST exhibited no obvious growth rate differences. Interestingly, induction of AhDGAT2a-GST and AhDGAT2b-GST proteins increased the sizes of the host cells by 2.4-2.5 times that of the controls (post-IPTG induction). The total fatty acid (FA) levels of the AhDGAT2a-GST and AhDGAT2a-GST transformants, as well as levels of C12:0, C14:0, C16:0, C16:1, C18:1n9c and C18:3n3 FAs, increased markedly, whereas C15:0 and C21:0 levels were lower than in non-transformed cells or those containing empty-vectors. In addition, the levels of some FAs differed between the two transformant strains, indicating that the two isozymes might have different functions in peanuts. This is the first time that a full-length recombinant peanut DGAT2 has been produced in a bacterial expression system and the first analysis of its effects on the content and composition of fatty acids in E. coli. Our results indicate that AhDGAT2 is a strong candidate gene for efficient FA production in E. coli. © 2013 Peng et al.


Kong L.,Shandong Academy of Agricultural Sciences | Wang F.,Shandong Academy of Agricultural Sciences | Wang F.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Si J.,Shandong Academy of Agricultural Sciences | And 4 more authors.
Journal of Plant Interactions | Year: 2013

Nitrogen availability is closely related to crop senescence and productivity, but its associated effect on reserve remobilization is not yet fully understood. In this study, we observed that nitrogen deficiency (N-) led to significant decreases in the activities of superoxide dismutase (SOD) (P<0.05), guaiacol peroxidase (P<0.05), and catalase (P<0.05) as well as a higher concentration of reactive oxygen species (ROS) (P<0.05) in wheat (Triticum aestivum L.) peduncles during the middle grain-filling compared with the application of 225 kg N ha-1 (N+). Callose concentration showed the same trend of temporal changes as ROS. Histochemical staining revealed that both ROS and callose predominantly occurred in vascular bundles of peduncles. Ultimately, grain filling rates and grain weight in N- wheat were reduced compared with N+ plant. These data suggest that the grain yield decline in N- wheat may be at least partially attributed to the higher callose deposition in peduncle vascular bundles and ROS level is closely associated with the increase in the callose deposition in wheat peduncle vascular bundles. © 2013 Copyright Taylor and Francis Group, LLC.


Yang S.,Shandong Academy of Agricultural Sciences | Yang S.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Wang F.,Shandong Academy of Agricultural Sciences | Wang F.,Shandong Agricultural University | And 8 more authors.
PLoS ONE | Year: 2013

Peanut is one of the calciphilous plants. Calcium (Ca) serves as a ubiquitous central hub in a large number of signaling pathways. The effect of exogenous calcium nitrate [Ca(NO3)2] (6 mM) on the dissipation of excess excitation energy in the photosystem II (PSII) antenna, especially on the level of D1 protein and the xanthophyll cycle in peanut plants under heat (40°C) and high irradiance (HI) (1 200 μmol m-2 s-1) stress were investigated. Compared with the control plants [cultivated in 0 mM Ca(NO3)2 medium], the maximal photochemical efficiency of PSII (Fv/Fm) in Ca2+-treated plants showed a slighter decrease after 5 h of stress, accompanied by higher non-photochemical quenching (NPQ), higher expression of antioxidative genes and less reactive oxygen species (ROS) accumulation. Meanwhile, higher content of D1 protein and higher ratio of (A+Z)/(V+A+Z) were also detected in Ca2+-treated plants under such stress. These results showed that Ca2+ could help protect the peanut photosynthetic system from severe photoinhibition under heat and HI stress by accelerating the repair of D1 protein and improving the de-epoxidation ratio of the xanthophyll cycle. Furthermore, EGTA (a chelant of Ca ion), LaCl3 (a blocker of Ca2+ channel in cytoplasmic membrane), and CPZ [a calmodulin (CaM) antagonist] were used to analyze the effects of Ca2+/CaM on the variation of (A+Z)/(V+A+Z) (%) and the expression of violaxanthin de-epoxidase (VDE). The results indicated that CaM, an important component of the Ca2+ signal transduction pathway, mediated the expression of the VDE gene in the presence of Ca to improve the xanthophyll cycle. © 2013 Yang et al.


Yang S.,Shandong Academy of Agricultural Sciences | Yang S.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Wang F.,Shandong Academy of Agricultural Sciences | Wang F.,Shandong Agricultural University | And 7 more authors.
Journal of Integrative Plant Biology | Year: 2015

In this study, we investigated the effects of exogenous calcium nitrate on photoinhibition and thylakoid protein level in peanut plants under heat (40°C) and high irradiance (HI) (1,200μmol/m2 per s) stress. Compared with control seedlings (cultivated in 0mmol/L Ca(NO3)2 medium), the maximal photochemical efficiency of photosystem II (PSII) in Ca2+-treated plants showed a slight decrease after 5h stress, accompanied by lower degree of PSII closure (1-qP), higher non-photochemical quenching, and lower level of membrane damage. Ca2+ inhibitors were used to analyze the varieties of antioxidant enzymes activity and PSII proteins. These results indicated that Ca2+ could protect the subunits of PSII reaction centers from photoinhibition by reducing the generation of reactive oxygen species. In the presence of both ethyleneglycol-bis(2-aminoethylether)-tetraacetic acid and ascorbic acid (AsA), the net degradation of the damaged D1 protein was faster than that only treated with AsA. Our previous study showed that either the transcriptional or the translational level of calmodulin was obviously higher in Ca2+-treated plants. These results suggested that, under heat and HI stress, the Ca2+ signal transduction pathway can alleviate the photoinhibition through regulating the protein repair process besides an enhanced capacity for scavenging reactive oxygen species. © 2014 The Authors. Journal of Integrative Plant Biology published by Wiley Publishing Asia Pty Ltd on behalf of Institute of Botany, The Chinese Academy of Sciences.


Kong L.,Shandong Academy of Agricultural Sciences | Si J.,Shandong Academy of Agricultural Sciences | Si J.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Zhang B.,Shandong Academy of Agricultural Sciences | And 7 more authors.
Australian Journal of Crop Science | Year: 2013

Wheat (Triticum aestivum L.) is one of the most important agricultural crops in China. In addition to the yield, the grain quality of wheat is central to the well-being of humans. Because of its high grain protein content (GPC), wheat is extensively applied in many foods and industrial uses. Currently, increasing grain quality is becoming a widely discussed topic in crop sciences. Wheat quality is directly affected by diverse environmental factors. However, the mechanisms of environmental effects are not fully elucidated, and experimental results are often inconsistent between regions or over years. In this review, we present the zoning of Chinese wheat quality based on key environmental variables and analyze the impacts of greatly varied environments on wheat GPC and related processing quality by summarizing the results of numerous field experiments. More importantly, this paper provides a number of potential strategies to increase the GPC and protein-based grain processing quality. Our objectives are to determine the general principles underlying how the environment affects wheat quality, facilitate further investigations of the environmental mechanisms that affect GPC, and thereby achieve good processing quality.


Zheng J.,Shandong Academy of Agricultural Sciences | Zheng J.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Wang Y.,Shandong Academy of Agricultural Sciences | Wang Y.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | And 8 more authors.
Plant Science | Year: 2014

S-like ribonucleases (S-like RNases) are homologous to S-ribonucleases (S-RNases), but are not involved in self-incompatibility. In dicotyledonous plants, S-like RNases play an important role in phosphate recycling during senescence and are induced by inorganic phosphate-starvation and in response to defense and mechanical wounding. However, little information about the functions of the S-like RNase in monocots has been reported. Here, we investigated the expression patterns and roles of an S-like RNase gene, OsRNS4, in abscisic acid (ABA)-mediated responses and phytochrome-mediated light responses as well as salinity tolerance in rice. The OsRNS4 gene was expressed at relatively high levels in leaves although its transcripts were detected in various organs. OsRNS4 expression was regulated by salt, PEG and ABA. The seedlings overexpressing OsRNS4 had longer coleoptiles and first leaves than wild-type seedlings under red light (R) and far-red light (FR), suggesting negative regulation of OsRNS4 in photomorphogenesis in rice seedlings. Moreover, ABA-induced growth inhibition of rice seedlings was significantly increased in the OsRNS4-overexpression ( OsRNS4-OX) lines compared with that in WT, suggesting that OsRNS4 probably acts as a positive regulator in ABA responses in rice seedlings. In addition, our results demonstrate that OsRNS4-OX lines have enhanced tolerance to high salinity compared to WT. Our findings supply new evidence on the functions of monocot S-like RNase in regulating photosensitivity and abiotic stress responses. © 2013 Elsevier Ireland Ltd.


Kong L.,Shandong Academy of Agricultural Sciences | Wang F.,Jinan Academy of Agricultural science | Zhang R.,Shandong Provincial Key Laboratory of Crop Genetic Improvement | Feng B.,Shandong Academy of Agricultural Sciences | And 3 more authors.
International Journal of Agriculture and Biology | Year: 2012

Pre-anthesis-synthesized proteins stored in the vegetative organs of cereal crops are re-mobilized and transferred to the grains. In this study, winter wheat (Triticum aestivum L.) was grown at levels of 225 kg N ha-1 (normal) and 330 kg N ha-1 (high) to investigate the effects of excessive N rates on the N re-mobilization from vegetative organs to the developing grains and on the grain protein yield. At a high N dose, the activities of the major flag leaf proteases were greatly decreased ~8-24 days after anthesis. From the beginning of the grain filling to the maturity, the post-anthesis flag leaf protein contents reduced by 80.50% and 70.40% at normal and high N rates, respectively (P < 0.05). The wheat flag leaves grown under high N conditions had a higher residual N at maturity than those grown under normal N conditions. Thus, up to maturity, the flag leaf N translocation efficiency was 67.4% in wheat exposed to a high N rate, significantly lower than the 73.8% of the plants exposed to a normal N rate (P < 0.05). Consequently, the grain protein content was not significantly increased with high N fertilization. In conclusion, the application of excessive N inhibits the proteolysis and decreases the export of flag leaf-stored protein to the developing grains and therefore, may not improve the protein yield in wheat. © 2012 Friends Science Publishers.

Loading Shandong Provincial Key Laboratory of Crop Genetic Improvement collaborators
Loading Shandong Provincial Key Laboratory of Crop Genetic Improvement collaborators