Shandong Provincial Key Laboratory for Dermatovenereology

Jinan, China

Shandong Provincial Key Laboratory for Dermatovenereology

Jinan, China
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Shi W.,Shandong Academy of Sciences | Shi W.,Shandong Provincial Key Laboratory for Dermatovenereology | Shi W.,Shandong University | Shi W.,Shandong Provincial Medical Center for Dermatovenereology | And 14 more authors.
Drug Design, Development and Therapy | Year: 2014

Background: Currently, 595 nm pulsed dye laser (PDL) therapy is offered as one of the effective treatments of port wine stains (PWSs). However, the efficacy of PDL differs in different populations.Objective: The purpose of the study was to investigate the efficacy, and related factors, of 595 nm PDL in the treatment of PWSs in Chinese patients with skin type III to IV.Methods: A total of 848 cases that were treated with PDL were enrolled and analyzed in this study. An independent dermatologist evaluated these lesions according to the before and after photographs.Results: The response rate (RR) of all the 848 PWS patients was 69.9%, within which the cure rate was 6.3%. The patients aged ≤1 year had the highest RR (93.9%), whereas those treated after age 50 reacted the worst (RR =25%). We analyzed the anatomical distribution of the lesion and found that the temporal region had the highest lesion clearance (RR =75.3%), while the extremities had the lowest clearance (RR =44.5%). Compared with the patients whose lesion size was larger than 80 cm2, the patients with small lesion size, of 0–20 cm2, had better clinical effect (RR =73.8% vs 53.2%). The reactions of the patients with hyperplastic lesion were worse than those with red patches (RR =36.4% vs 71.7%). As well, increasing treatment numbers could achieve higher clearance rates (P=0.005).Conclusion: The PDL had a relatively high RR but a low clearance rate in Chinese patients with PWS, although the earlier the intervention, the better was the efficacy. The response of PDL was, not only related to the anatomical area, but also, to the lesion size, type of lesion (ie, the presence of existing hyperplastic lesions), and the number of treatment, all of which are essential for the evaluation of therapeutic effect and acquisition of patients consent before treatment. © 2014 Shi et al.


Wang N.,Shandong University | Wang N.,Shandong Provincial Key Laboratory for Dermatovenereology | Wang N.,Shandong Provincial Medical Center for Dermatovenereology | Parimi L.,Shandong University | And 7 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2017

Dapsone is a bactericidal and bacteriostatic against Mycobacterium leprae, a causative agent of leprosy. Dapsone is also applied in a range of medical fields because of its anti-inflammatory and immunomodulatory effects. Dapsone hypersensitivity syndrome (DHS) is a rare yet serious adverse drug reaction (ADR) caused by dapsone involving multiple organs. We performed a systematic review of published articles describing dapsone-induced hypersensitivity syndrome, including all Chinese articles and the latest literature available in online databases published between October 2009 and October 2015. We determined the prevalence, clinical characteristics, and mortality rate of DHS. Importantly, we also summarized the recent advances in genetic testing allowing prediction of ADRs. In an initial systematic electronic search, we retrieved 191 articles. Subsequently, these articles were further filtered and ultimately 84 articles (60 Chinese case reports, 21 non-Chinese articles, and three epidemiological studies) were selected, which included 877 patients. The prevalence of DHS among Chinese patients was 1.5% with a fatality rate of 9.6%. Early withdrawal of dapsone and appropriate treatment reduced the fatality rate. Most importantly, genetic screening for the HLA-B13:01 allele among high-risk populations showed a significant utility as a useful genetic marker to DHS. In conclusion, this review discusses the epidemiological and clinical characteristics of DHS among Chinese patients, which may help physicians to understand this syndrome. Copyright © 2017 by The American Society of Tropical Medicine and Hygiene.


Ma H.-L.,Anhui Medical University | Ma H.-L.,Yantaishan Hospital | Zhao X.-F.,Yantaishan Hospital | Chen G.-Z.,Qingdao University | And 6 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Dermal fibrosis is characterized by collagen accumulation and hyperproliferation of fibroblasts. NLRC5, as the largest member of nucleotide-binding domain and leucine-rich repeat (NLRs) family, has recently been implicated in the development of hepatic fibrosis. However, the role of NLRC5 in dermal fibrosis remains unknown. Therefore, herein, we investigated the effects of NLRC5 on keloid fibroblasts (KFs) and transforming growth factor-β1 (TGF-β1)-induced collagen expression and explored the underlying mechanism. We observed that NLRC5 mRNA and protein levels were highly expressed in KFs, silencing NLRC5 greatly suppressed TGF-β1-induced KFs proliferation. Silencing NLRC5 also obviously inhibited the expression of type I collagen, CTGF and α-smooth muscle actin (α-SMA) in human KFs induced by TGF-β1, as well as the expression of TGF-β receptor I and II. Furthermore, silencing NLRC5 suppressed the expression of TGF-β1-induced Smad2 and Smad3 phosphorylation in human KFs. Taken together, our study suggest that silencing NLRC5 reduced ECM expression in KFs through inhibiting the TGF-β1/Smad signaling pathway. Therefore, NLRC5 may represent a promising target for treatment of the keloid disease. © 2016 Elsevier Masson SAS


Liu H.,Shandong Academy of Sciences | Liu H.,Shandong University | Liu H.,Shandong Provincial Key Laboratory for Dermatovenereology | Liu H.,Shandong Provincial Medical Center for Dermatovenereology | And 61 more authors.
American Journal of Human Genetics | Year: 2012

Of eight leprosy susceptibility loci identified by genome-wide association studies, five have been implicated in Crohn disease, suggesting a common genetic fingerprint between leprosy and inflammatory bowel disease (IBD). Here, we conducted a multiple-stage genetic association study of 133 IBD susceptibility loci in multiple leprosy samples (totaling 4,971 leprosy cases and 5,503 controls) from a Chinese population and discovered two associations at rs2058660 on 2q12.1 (p = 4.57 × 10-19; odds ratio [OR] = 1.30) and rs6871626 on 5q33.3 (p = 3.95 × 10-18; OR = 0.75), implicating IL18RAP/IL18R1 and IL12B as susceptibility genes for leprosy. Our study reveals the important role of IL12/IL18-mediated transcriptional regulation of IFN-γ production in leprosy, and together with previous findings, it demonstrates the shared genetic susceptibility between infectious and inflammatory diseases. © 2012 The American Society of Human Genetics.


Zhang F.,Shandong Academy of Sciences | Zhang F.,Shandong University | Zhang F.,Shandong Provincial Key Laboratory for Dermatovenereology | Zhang F.,Shandong Provincial Medical Center for Dermatovenereology | And 63 more authors.
Nature Genetics | Year: 2011

We performed a genome-wide association study with 706 individuals with leprosy and 5,581 control individuals and replicated the top 24 SNPs in three independent replication samples, including a total of 3,301 individuals with leprosy and 5,299 control individuals from China. Two loci not previously associated with the disease were identified with genome-wide significance: rs2275606 (combined P = 3.94 × 10 -14, OR = 1.30) on 6q24.3 and rs3762318 (combined P = 3.27 × 10 -11, OR = 0.69) on 1p31.3. These associations implicate IL23R and RAB32 as new susceptibility genes for leprosy. Furthermore, we identified evidence of interaction between the NOD2 and RIPK2 loci, which is consistent with the biological association of the proteins encoded by these genes (NOD2-RIPK2 complex) in activating the NF-κB pathway as a part of the host defense response to infection. Our findings have expanded the biological functions of IL23R by uncovering its involvement in infectious disease susceptibility and suggest a potential involvement of autophagocytosis in leprosy pathogenesis. The IL23R association supports previous observations of the marked overlap of susceptibility genes for leprosy and Crohn's disease, implying common pathogenesis mechanisms. © 2011 Nature America, Inc. All rights reserved.


Zhang F.,Shandong Academy of Sciences | Zhang F.,Shandong Provincial Hospital for Skin Diseases | Zhang F.,Shandong Provincial Key Laboratory for Dermatovenereology | Zhang F.,Shandong Provincial Medical Center for Dermatovenereology | And 22 more authors.
Journal of the European Academy of Dermatology and Venereology | Year: 2012

Background Although dermatitis herpetiformis (DH) is a relatively common disease in Caucasian populations, it is very rare in the Far East, including China. Objectives To investigate the clinical, histological and immunopathological characteristics of Chinese patients with DH. Methods The clinical data and serum samples of patients with DH diagnosed at Shandong Provincial Institute of Dermatology and Venereology from 2006 to 2010 were collected. Anti-endomysium antibodies (EMA) and anti-tissue transglutaminase (tTG) antibodies were tested using indirect immunofluorescence and enzyme-linked immunosorbent assay respectively. Biopsies of small intestine were performed in those patients who had given informed consent. Results The study group consisted of 22 patients with DH. The male to female ratio was 2.1: 1; the average age was 44 years. Five patients reported mild gastrointestinal symptoms. Dapsone was used to treat 14 patients, who achieved rapid relief. Direct immunofluorescence showed granular IgA deposits at the papillary tips and/or basement membrane zone in 95.5% (21/22) patients. Fibrillar IgA deposition limited to the papillary tips was also seen in one patient (4.5%). IgA antibodies against EMA and tTG were found in 50% (8/16) and 62.5% (10/16) of patient sera respectively. Small-intestinal biopsies of two patients confirmed the presence of coeliac disease. Conclusions The clinical, histological and immunopathological characteristics of Chinese patients with DH are similar to those seen in Caucasian populations. The positive rates of anti-EMA and anti-tTG antibodies were lower compared with those seen in Western countries. DH is not extremely rare in China and should be actively investigated. © 2011 European Academy of Dermatology and Venereology.


Yang B.,Shandong Academy of Sciences | Yang B.,Shandong Provincial Key Laboratory for Dermatovenereology | Wang C.,Shandong Academy of Sciences | Wang C.,Shandong Provincial Key Laboratory for Dermatovenereology | And 12 more authors.
Australasian Journal of Dermatology | Year: 2014

A 22-year-old primigravida had a pruritic, erythematous, bullous eruption on the skin during the 26th week of gestation. After delivery the eruption flared up. The diagnosis of pemphigoid gestationis was confirmed based on histopathological and immunofluorescence findings. The result of immunoblotting showed IgG autoantibodies which reacted against BP230 in epidermal extracts and 290 kDa type VII collagen in dermal extracts. The BP180 antibodies were also detected by an enzyme-linked immunosorbent assay BP180NC16a diagnosis kit. Pulsed corticosteroid and cyclophosphamide resulted in a favourable response at the acute stage. The patient was cured in 2 years. The analysis of the patient's autoantibodies provides strong evidence for the involvement of epitope spreading in her autoimmune disease. © 2012 The Australasian College of Dermatologists.

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