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Wang Y.-B.,Shandong Provincial Institute of Parasitic Diseases | Wang Y.-B.,University of Jinan | Kong X.-L.,Shandong Provincial Institute of Parasitic Diseases | Zhang B.-G.,Shandong Provincial Institute of Parasitic Diseases | And 5 more authors.
Chinese Journal of Schistosomiasis Control | Year: 2013

Objective: To understand the characteristics of malaria prevalence and epidemic in Shandong Province in 2012 so as to provide the evidence for improving the work of the elimination of malaria. Methods: The epidemiological data of malaria cases collected from the Disease Surveillance Information Reporting System of Chinese Center for Disease Control and Prevention were analyzed with the descriptive epidemiological method for epidemiological characteristics of malaria. Results: A total of 93 malaria cases were reported in Shandong Province in 2012 with the incidence of 0.097 per 100 thousand, with a reduction of 19.83% as compared to 2011. There were 93 imported cases which decreased by 4.12% compared with 97 cases in 2011 and it was the first year that there was no local infection. Jining, Qingdao and Weihai cities reported more cases, with 62.37% (58/93) of the total number of the whole province. Totally 93.55% of malaria cases were imported from Africa, most from Equatorial Guinea, Nigeria and Angola. There were 3 cases of imported ovale malaria firstly reported. Conclusions: There were no local malaria cases reported in Shandong Province in 2012, but the imported malaria prevention and control was still not optimistic. In order to achieve the goal of malaria elimination in Shandong Province, it needs to continue to strengthen epidemic management, professional training and work supervision, strengthen management, advocacy and detection on the floating population, and explore multisectoral coordination mechanisms.


Xing L.-L.,U.S. Center for Disease Control and Prevention | Wang Y.-B.,Shandong Provincial Institute of Parasitic Diseases | Cui X.-F.,U.S. Center for Disease Control and Prevention | Bu X.-Q.,Shandong Provincial Institute of Parasitic Diseases | And 4 more authors.
Chinese Journal of Schistosomiasis Control | Year: 2013

Objective: To analyze the endemic situation of malaria in Shanxian County, Shandong Province, China in the last 10 years, so as to understand the epidemiological characteristics of the disease and provide the evidence for the establishment of control strategy to eliminate malaria. Methods: The data related to the endemic situation of malaria in Shanxian County from 2002 to 2011 were collected and analyzed. Results: From 2002 to 2011, 125 cases of malaria were reported, and the annual incidences were in the range of 0.18/100 000-2.00/100 000. Totally 60.80% of the cases focused on 3 townships, namely Fugang, Huanggang and Caozhuang. All the 125 cases were new vivax malaria cases, among them, 121 were locally infected, and the other 4 were imported. The epidemic season was from June to October, and the peak time was from July to September. There were more male cases than female cases. The ages of the cases were from 1 to 83 years old, and the incidence in those aged from 46-60 years old was higher. Conclusions: The malaria incidence in Shanxian County is on the rise, and the 3 townships in the south are the key areas for control. The control measures including the surveillance of floating population and mosquitoes, and malaria control health education should be strengthened.


PubMed | Shandong Provincial Institute of Parasitic Diseases and U.S. Center for Disease Control and Prevention
Type: Journal Article | Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control | Year: 2013

To analyze the endemic situation of malaria in Shanxian County, Shandong Province, China in the last 10 years, so as to understand the epidemiological characteristics of the disease and provide the evidence for the establishment of control strategy to eliminate malaria.The data related to the endemic situation of malaria in Shanxian County from 2002 to 2011 were collected and analyzed.From 2002 to 2011, 125 cases of malaria were reported, and the annual incidences were in the range of 0.18/100,000-2.00/100,000. Totally 60.80% of the cases focused on 3 townships, namely Fugang, Huanggang and Caozhuang. All the 125 cases were new vivax malaria cases, among them, 121 were locally infected, and the other 4 were imported. The epidemic season was from June to October, and the peak time was from July to September. There were more male cases than female cases. The ages of the cases were from 1 to 83 years old, and the incidence in those aged from 46-60 years old was higher.The malaria incidence in Shanxian County is on the rise, and the 3 townships in the south are the key areas for control. The control measures including the surveillance of floating population and mosquitoes, and malaria control health education should be strengthened.


Liu Q.,Shandong Provincial Institute of Parasitic Diseases | Yin K.,Shandong Provincial Institute of Parasitic Diseases | Zhu S.,Shandong Provincial Institute of Parasitic Diseases | Zhang L.,Shandong Academy of Sciences | And 5 more authors.
BioScience Trends | Year: 2010

C35 was reported to be a new biomarker and therapeutic target for breast cancer. To explore the functional importance of C35, we constructed small interfering RNA (siRNA) targeting C35 and investigated the effects of the siRNAs on C35 expression and apoptosis of T47D cells. C35 siRNAs were constructed and named psiRNA-C35-1 and psiRNA-C35-2. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blots were used to detect the effects of the siRNAs on mRNA and protein expression of C35 in T47D cells. The effects of the two siRNAs on apoptosis of T47D cells were detected by flow cytometry and terminal dUTP nicked-end labelling assays. Also, the apoptosis related molecule caspase-3 was detected using Western blots. The psiRNA-C35-1 and psiRNA-C35-2 siRNAs were verified by both EcoR I/Hind III digestion analysis and automated DNA sequencing. RTPCR and Western blots showed that C35 mRNA and protein expression in T47D cells were obviously inhibited after psiRNA-C35-1 and psiRNA-C35-2 transfection. Flow cytometry and terminal dUTP nicked-end labelling assays showed that apoptosis of T47D cells was significantly induced after transfection with psiRNA-C35-1 and psiRNA-C35-2 (p < 0.05). Also, caspase-3 expression in the psiRNA-C35-1 and psiRNA-C35-2 transfected cells was obviously higher than that of the Lipofectamine and pTZU6+1 transfected cells. This study showed that apoptosis of T47D cells can be significantly induced by inhibiting C35 expression using siRNAs, which may be caused by activating caspase-3. C35 might play an important role in apoptosis of breast cancer cells, and therapeutic strategies targeting C35 may be useful for breast cancer treatment.


Wang Z.-L.,Shandong Provincial Institute of Parasitic Diseases | Wang Y.-R.,Tongji University | Fu T.-X.,Shandong Provincial Institute of Parasitic Diseases | Mao D.-H.,Shandong Provincial Institute of Parasitic Diseases
Chinese Journal of Schistosomiasis Control | Year: 2013

Objective: To explore the clinical characteristics, early diagnosis, and treatment of patients with imported falciparum malaria from Africa. Methods: The clinical data of 91 imported falciparum malaria cases were analyzed by retrospective study. Results: All the 91 cases had the history of mosquito bites. The clinical manifestation of these cases varied, including fever, headache, chill, diarrhea, anemia, thrombocytopenia, proteinuria, damage of liver function, abdominal ultrasonographic presentations (enlarged liver and spleen). All the patients were successfully treated with the combination therapy of artemether and primaquine. Conclusion: The key procedures for treating imported falciparum malaria are earlier diagnosis and effective therapy. The combination therapy with artemether and primaquine shows a high efficacy and low side effect and low relapsed rate.


PubMed | Shandong Provincial Institute of Parasitic Diseases
Type: Journal Article | Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control | Year: 2014

To understand the characteristics of malaria prevalence and epidemic in Shandong Province in 2012 so as to provide the evidence for improving the work of the elimination of malaria.The epidemiological data of malaria cases collected from the Disease Surveillance Information Reporting System of Chinese Center for Disease Control and Prevention were analyzed with the descriptive epidemiological method for epidemiological characteristics of malaria.A total of 93 malaria cases were reported in Shandong Province in 2012 with the incidence of 0.097 per 100 thousand, with a reduction of 19.83% as compared to 2011. There were 93 imported cases which decreased by 4.12% compared with 97 cases in 2011 and it was the first year that there was no local infection. Jining, Qingdao and Weihai cities reported more cases, with 62.37% (58/93) of the total number of the whole province. Totally 93.55% of malaria cases were imported from Africa, most from Equatorial Guinea, Nigeria and Angola. There were 3 cases of imported ovale malaria firstly reported.There were no local malaria cases reported in Shandong Province in 2012, but the imported malaria prevention and control was still not optimistic. In order to achieve the goal of malaria elimination in Shandong Province, it needs to continue to strengthen epidemic management, professional training and work supervision, strengthen management, advocacy and detection on the floating population, and explore multisectoral coordination mechanisms.


PubMed | Shandong Provincial Institute of Parasitic Diseases
Type: Journal Article | Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control | Year: 2013

To explore the clinical characteristics, early diagnosis, and treatment of patients with imported falciparum malaria from Africa.The clinical data of 91 imported falciparum malaria cases were analyzed by retrospective study.All the 91 cases had the history of mosquito bites. The clinical manifestation of these cases varied, including fever, headache, chill, diarrhea, anemia, thrombocytopenia, proteinuria, damage of liver function, abdominal ultrasonographic presentations (enlarged liver and spleen). All the patients were successfully treated with the combination therapy of artemether and primaquine.The key procedures for treating imported falciparum malaria are earlier diagnosis and effective therapy. The combination therapy with artemether and primaquine shows a high efficacy and low side effect and low relapsed rate.


PubMed | Shandong Provincial Institute of Parasitic Diseases
Type: Journal Article | Journal: Bioscience trends | Year: 2010

C35 was reported to be a new biomarker and therapeutic target for breast cancer. To explore the functional importance of C35, we constructed small interfering RNA (siRNA) targeting C35 and investigated the effects of the siRNAs on C35 expression and apoptosis of T47D cells. C35 siRNAs were constructed and named psiRNA-C35-1 and psiRNA-C35-2. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blots were used to detect the effects of the siRNAs on mRNA and protein expression of C35 in T47D cells. The effects of the two siRNAs on apoptosis of T47D cells were detected by flow cytometry and terminal dUTP nicked-end labelling assays. Also, the apoptosis related molecule caspase-3 was detected using Western blots. The psiRNA-C35-1 and psiRNA-C35-2 siRNAs were verified by both EcoR I/Hind III digestion analysis and automated DNA sequencing. RT-PCRs and Western blots showed that C35 mRNA and protein expression in T47D cells were obviously inhibited after psiRNA-C35-1 and psiRNA-C35-2 transfection. Flow cytometry and terminal dUTP nicked-end labelling assays showed that apoptosis of T47D cells was significantly induced after transfection with psiRNA-C35-1 and psiRNA-C35-2 (p < 0.05). Also, caspase-3 expression in the psiRNA-C35-1 and psiRNA-C35-2 transfected cells was obviously higher than that of the Lipofectamine and pTZU6+1 transfected cells. This study showed that apoptosis of T47D cells can be significantly induced by inhibiting C35 expression using siRNAs, which may be caused by activating caspase-3. C35 might play an important role in apoptosis of breast cancer cells, and therapeutic strategies targeting C35 may be useful for breast cancer treatment.

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