Shandong Provincial Engineering Center on Environmental Science and Technology

Jingshi, China

Shandong Provincial Engineering Center on Environmental Science and Technology

Jingshi, China
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Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | Jiang L.,Shandong University | Hou Q.,Shandong University | Yu Z.,Shandong University
Biotechnology for Biofuels | Year: 2017

Background: Although numerous studies have used wastewater as substitutes to cultivate microalgae, most of them obtained weaker algal viability than standard media. Some studies demonstrated a promotion of phytohormones on algal growth in standard media. For exploiting a strategy to improve algal biomass accumulation in effluent from anaerobic digestion of kitchen waste (ADE-KW), the agricultural phytohormones gibberellin, indole-3-acetic acid, and brassinolide (GIB) were applied to Chlorella SDEC-11 and Scenedesmus SDEC-13 at different stages of algal growth. Previous studies have demonstrated a promotion of phytohormones on algal growth in standard media, but attempts have been scarce, focusing on wastewater cultivation system. In addition, the effects of wastewater on algal morphology and ultrastructure have not been revealed so far, much less on the mechanism of the role of phytohormones on algae. Results: ADE-KW disrupted the membranes of nuclear and chloroplast in ultrastructural cell of SDEC-11, and reduced the room between chloroplast and cell membrane and increased the starch size of SDEC-13. This reduced algal growth and biocompound accumulation, but SDEC-13 had greater adaptation to ADE-KW than SDEC-11. Moreover, inoculation with an algal seed pretreated with GIB aided the adaptability and viability of algae in ADE-KW, which for SDEC-13 was even promoted to the level in BG11. GIB mitigated the inhibition of ADE-KW on algal cell division and photosynthetic pigments and apparatus, and increased lipid droplets, which might result from the change in the synthesis and the fate of nicotinamide adenine dinucleotide phosphate. GIB addition significantly promoted lipid productivity of the two algal species, following 13 mg L-1 d-1 of SDEC-11 in B+ADE-KW and especially 13 mg L-1 d-1 of SDEC-13 achieved during the priming of algal seed with the hormones, which is 139% higher than 5 mg L-1 d-1 achieved in ADE-KW control. Conclusions: Agricultural phytohormones could be applied as a strategy for promoting biomass and biocompound accumulation of algae in ADE-KW, in which pretreatment of the algal inoculum with hormones is a unique way to help algae survive under stress. Considering our results and treatment technology for kitchen waste, a more feasible and economic plant can be built incorporating anaerobic digestion, algae cultivation with ADE-KW assisted with phytohormones, and biodiesel production. © 2017 The Author(s).


Ma G.,Shandong University | Ma G.,Shandong Jianzhu University | Hu W.,Shandong University | Hu W.,Shandong Provincial Engineering Center on Environmental Science and Technology | And 5 more authors.
Energy Conversion and Management | Year: 2015

Heterogeneous KF/CaO catalyst, prepared by the wet impregnation method with different KF loadings (15-35 wt.%) and calcination temperatures (600-1000°C), was used to assist biodiesel production from microalgae Chlorella vulgaris under the combination of ultrasound and microwave (US-MW) irradiation. It was found that the catalyst calcinated at 900°C with 25 wt.% KF loading presented the optimum performance, when the test was conducted with 12 wt.% of catalyst and a methanol to biomass ratio of 8:1 at 60°C for 45 min. The highest fatty acid methyl esters (FAME) yield was 93.07 ± 2.39%, which was significantly higher than that achieved by using US or MW irradiation individually. The catalysts were characterized by X-ray diffraction, scanning electron microscopy and Brunauer-Emmett-Teller. © 2014 Elsevier Ltd. All rights reserved.


Meng P.,Shandong University | Meng P.,Taishan University | Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | And 4 more authors.
Bioresource Technology | Year: 2014

Microorganisms play a vital role in degradation of multiple pollutants in constructed wetlands (CWs). Thus, the search for methods to improve microbial degradation in CWs is crucial. This study provides a review of critical parameters including availability of organic carbon, redox condition, temperature, pH, presence of plants, media characteristics and their influences on microbial processes. Current strategies focusing on regulation of carbon source, redox condition, and choice of substrates to enhance microbial activity in CWs are also described. A special emphasis is given to the application of bioaugmentation to enhance microbial activities in wetland in future research. © 2014 Elsevier Ltd.


Song M.,Shandong University | Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | Hu W.,Shandong University | And 2 more authors.
Bioresource Technology | Year: 2013

In this study, the potential of 10 algae species for biodiesel production were evaluated by determining their fatty acid profiles, biodiesel properties besides growth rate, biomass concentration and lipid productivity. Among seven strains with high growth and lipid accumulation properties, excluding Kirchneriella lunaris and Lyngbya kuetzingii, five species Selenastrum capricornutum, Chlorella vulgaris, Scenedesmus obliqnus, Phaeodactylum tricornutum and Isochrysis sphacrica were finally selected for biodiesel production due to their possessing higher lipid productivity and favorable biodiesel properties. The best strain was P.tricornutum, with lipid content of 61.43±0.95%, lipid productivity of 26.75mgL-1 d-1, the favorable fatty acid profiles of C16-C18 (74.50%), C14:0 (11.68%) and C16:1 (22.34%) as well as suitable biodiesel properties of higher cetane number (55.10), lower iodine number (99.2gI2/100g) and relatively low cloud point (4.47°C). © 2013 Elsevier Ltd.


Sun F.,Shandong University | Pei H.-Y.,Shandong University | Pei H.-Y.,Shandong Provincial Engineering Center on Environmental Science and Technology | Hu W.-R.,Shandong University | And 4 more authors.
Separation and Purification Technology | Year: 2013

The damage of cyanobacterial cells and the release of their intracellular microcystins (MCs) present an enormous threat to drinking water. Effective control of cyanobacteria both in coagulation and floc storage processes plays an important significance in avoiding the additional release of MCs during water treatment. In this paper, the cell integrity acted as a key factor to assess the damage of polyaluminum chloride (PACl) coagulation/flocculation process on Microcystis aeruginosa cells. Effects of coagulant dose, coagulation stirring and floc storage time were comprehensively evaluated to regulate M. aeruginosa cell lysis and MCs release. Results showed that all cells were removed intactly by the surface charge neutralization with PACl in the coagulation process. While in floc storage process, PACl caused obvious damage to cells and led to a large amount of MCs release after the protective effects of EPSs produced by M. aeruginosa cells were destroyed or decomposed. Coagulation stirring could cause cells lyse earlier than the natural lysis, but this damage was slighter than that of coagulant PACl, which obviously aggravate the cell lysis of M. aeruginosa in flocs after 2 days. This study is not only significant for the effective removal of cyanobacterial cells from drinking water sources but instructive for the prevention of secondary pollution of MCs during water recirculation from the sludge treatment to the head of the process. © 2013 Elsevier B.V. All rights reserved.


Han F.,Shandong University | Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | Hu W.,Shandong University | And 4 more authors.
Energy Conversion and Management | Year: 2015

Specifically growth-state-based schemes for cultivation optimization of microalgae Chlorella sp. were researched for the lipid enhancement in biodiesel production, compared with common optimization experiments. The optimized conditions of initial cell density, light intensity and aeration flow in common optimization were about 0.45 cm-1 (in OD686, i.e. 0.15 g/L), 6000 lux and 0.2 vvm (mL mL-1 min-1) respectively, taking into account the lipid yield and energy consumption. The maximum dry weight and lipid content were 1.2 g/L and 33.6% respectively. Then three specific schemes for cultivation conditions adjustment were conducted to study the effects of changing conditions along with growth state for the potential enhancement of biomass and lipid production. The results showed that excellent enhancement of both biomass dry weight of 1.9 g/L and lipid yield of 0.6 g/L was achieved. The new and specific cultivation method significantly improved the biomass and lipid production by 52.7% and 64.7% respectively, compared with the aforementioned three common optimization experiments. The GC-MS analysis indicated that C16-C18 components accounted for more than 90% of fatty acid, and the fatty acid had better properties than that in common cultivation. © 2014 Elsevier Ltd. All rights reserved.


Pei H.-Y.,Shandong University | Pei H.-Y.,Shandong Provincial Engineering Center on Environmental Science and Technology | Ma C.-X.,Shandong University | Hu W.-R.,Shandong University | And 2 more authors.
Bioresource Technology | Year: 2014

This work aimed to study the effects of chitosan on cell integrity and extracellular microcystins (MCs) of Microcystis aeruginosa cells during flocculation and flocs storage processes. The impacts of chitosan addition, flocculation stirring and flocs storage time were comprehensively detected to prevent or reduce cell lysis and MCs release. Response surface method (RSM) was applied to optimize the chitosan flocculation. Under chitosan concentration 7.31. mg/L and optimized mechanical conditions, 99% of M. aeruginosa cells were integrated removed. Furthermore, amounts of extracellular MCs were adsorbed by chitosan polymers in this process. With chitosan flocs protect, though cells showed some damage, extracellular MCs concentration in flocculated samples lower than background level within first 2. d. However, lots of MCs release was observed after 4. d which may result from chitosan degradation and cells lysis. Therefore, chitosan flocs should be treated within 2. d to prevent the adsorbed MCs releasing again. © 2013 Elsevier Ltd.


Ji Y.,Shandong University | Hu W.,Shandong University | Hu W.,Shandong Provincial Engineering Center on Environmental Science and Technology | Li X.,Shandong University | And 4 more authors.
Bioresource Technology | Year: 2014

Monosodium glutamate wastewater (MSGW) is a potential medium for microbial cultivation because of containing abundant organic nutrient. This paper seeks to evaluate the feasibility of growing Chlorella vulgaris with MSGW and assess the influence of MSGW concentration on the biomass productivity and biochemical compositions. The MSGW diluted in different concentrations was prepared for microalga cultivation. C. vulgaris growth was greatly promoted with MSGW compared with the inorganic BG11 medium. C. vulgaris obtained the maximum biomass concentration (1.02. g/L) and biomass productivity (61.47. mg/L. d) with 100-time diluted MSGW. The harvested biomass was rich in protein (36.01-50.64%) and low in lipid (13.47-25.4%) and carbohydrate (8.94-20.1%). The protein nutritional quality and unsaturated fatty acids content of algal increased significantly with diluted MSGW. These results indicated that the MSGW is a feasible alternative for mass cultivation of C. vulgaris. © 2013 Elsevier Ltd.


Xu H.,Shandong University | Xiao H.,Shandong University | Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | And 3 more authors.
Microporous and Mesoporous Materials | Year: 2015

Hydrogen-terminated porous Si wafers with (1 0 0) and (1 1 1) oriented crystal planes were fabricated through a photo-electrochemical etching. It is found that the porosity of silicon wafers and their etch rates are determined as a function of crystal orientation. Due to the anisotropic etching behavior of single-crystal silicon, the hydrogen-terminated porous Si (1 0 0) wafers exhibit not only more excellent photodegradation activity but also stronger stability for methyl orange degradation than hydrogen-terminated porous Si (1 1 1) wafers under visible light irradiation. For the unetched Si wafers, however, the photodegradation activities of methyl orange exhibit a contrary conclusion. © 2014 Elsevier Inc. All rights reserved.


Xu H.,Shandong University | Pei H.,Shandong University | Pei H.,Shandong Provincial Engineering Center on Environmental Science and Technology | Xiao H.,Shandong University | And 2 more authors.
AIP Advances | Year: 2015

Si is well-known semiconductor that has a fundamental bandgap energy of 1.12 eV. Its photogenerated electrons in the conduction band can react with the ubiquitous oxygen molecules to yield O2- radicals, but the photogenerated holes in the valance band can't interact with OH- to produce OH radicals. In this paper, we study the degradation of methyl orange (MO) by hydrogen-terminated porous Si (H-PSi) in the presence and in the absence of light. The absorption spectra of the degraded MO solutions indicated that the H-PSi had superior degradation ability. In the dark, the reduction of dye occurs simply by hydrogen transfer. Under room light, however, some of the dye molecules can be reduced by hydrogen transfer first and then decomposed in the conduction and valance bands. This result should be ascribed to its wide band gap energies centered at 1.79-1.94 eV. © 2015 Author(s).

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