Hou Q.,Shandong Provincial Chest Hospital |
Zhao C.,Shandong Provincial Chest Hospital |
Wang X.,Shandong Corps Hospital of Chinese Peoples Armed Police Forces |
Shi H.,Shandong Provincial Chest Hospital
International Journal of Clinical and Experimental Pathology | Year: 2017
Bmi-1, a key member of the Polycomb group complex, has been shown in non-small cell lung cancer (NSCLC) tumorigenesis and progress. The aim of this study was to detect the levels of circulating Bmi-1 mRNA in plasma of NSCLC patients and evaluate its diagnostic and prognostic value. Levels of circulating Bmi-1 mRNA were detected by reverse transcription quantitative real-time PCR in plasma of 108 NSCLC patients and 40 healthy controls. The results showed that circulating Bmi-1 mRNA levels were significantly upregulated in plasma of NSCLC patients when compared with healthy controls (P < 0.001), and significantly correlated with tumor size (P=0.038) and clinical stage (P=0.025). The receiver operating characteristics (ROC) curve analyses revealed that circulating Bmi-1 mRNA had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.805 with 72.9% sensitivity and 69.1% specificity in discriminating NSCLC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating Bmi-1 mRNA level and reduced overall survival (P=0.028). Cox analysis indicated that it was an independent prognostic factor for NSCLC. In conclusion, our findings suggested that circulating Bmi-1 mRNA might be a promising non-invasive diagnosis and prognosis biomarker for NSCLC.
Wu Y.-H.,Shandong Provincial Chest Hospital |
Wang X.-F.,Shandong Provincial Chest Hospital |
Wang M.-S.,Shandong Provincial Chest Hospital
European Review for Medical and Pharmacological Sciences | Year: 2015
OBJECTIVE: Pleural effusion (PE) adenosine deaminase (ADA) has good performance in detection of tuberculous pleural effusion (TPE). However, few study was conducted for its value in pediatric patients. To evaluate PE ADA in diagnosis of pediatric TPE, a retrospective study was performed. PATIENTS AND METHODS: 204 pediatric PE patients were enrolled, and then were grouped into TPE group (77 cases, aged 11.51 ± 0.40 years) and non-TPE group (127 cases, aged 6.39 ± 0.35 years). Man-Whitney U test was used to compare difference in pleural ADA between the two groups. The correlation between age and ADA activity was analyzed by Spearman's correlation coefficient analysis. RESULTS: In our study, there was no difference in pleural ADA between TPE (62.1 ± 4.2 U/L) and non-TPE patients (87.7 ± 10.0 U/L). Compared with empyema patients (183.8 ± 30.0 U/L), pleural ADA was lower in parapneumonic effusion (PPE) patients (63.4 ± 3.8, p < 0.01), or TPE patients (p <0.01). Correlation analysis showed that there were no correlation between age and pleural ADA within TPE, PPE or both patients (all p > 0.05). Meanwhile, there was no significant difference in PE ADA level between genders. CONCLUSIONS: Considering the fact that the majority of pediatric PEs is TPE and PPE, our study suggests that PE ADA isn't accurate in detection of pediatric TPE. Meanwhile, an extremely high ADA activity should raise suspicion of empyema or lymphoma.
Xu L.,Shandong Academy of Sciences |
Tian J.,Shandong Provincial Chest Hospital |
Liu Y.,Shandong Academy of Sciences |
Li C.,Shandong University
Journal of Magnetic Resonance Imaging | Year: 2014
Purpose To prospectively evaluate the accuracy of diffusion-weighted (DW) magnetic resonance (MR) imaging with background signal suppression (MR-DWIBS) for detecting mediastinal lymph node metastasis of nonsmall-cell lung cancer (NSCLC). Materials and Methods MR-DWIBS was performed in 42 consecutive patients (27 men, 15 women; age range, 42-78 years; median age, 55 years) with histologically proven NSCLC. The visualization rate of metastatic lymph node (MLN) and benign lymph node (BLN) of enlarged lymph nodes (ELN) and normal-sized lymph nodes (NLN) was compared by using a chi-square test or Fisher's exact test on a per-nodal basis. Apparent diffusion coefficient (ADC) of MLN and BLN was measured and compared by using two-tailed unpaired Student's t-test. Receiver operating characteristic (ROC) analysis was used to assess the overall diagnostic accuracy of ADC for ELN and NLN. The optimal cutoff value was determined and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy was calculated. Results Thirty-five out of 119 lymph resected nodes were confirmed to be metastatic by histologic examination. The visualization rate of MLN was significantly higher than that of BLN for ELN (P < 0.001) and for NLN (χ2 = 7.506, P = 0.006). For both ELN and NLN, ADC of MLN was significantly lower than that of BLN (t = -5.380, P < 0.001 and t = -6.435, P < 0.001). ADC was significant for detection of MLN for both ELN (Az = 0.975, P < 0.001) and NLN (Az = 0.919, P < 0.001). For NLN, the optimal cutoff value of ADC was 2.04 mm2/s, where the sensitivity, specificity, PPV, NPV, and accuracy were 75.0%, 90.9%, 66.7%, 93.8%, and 87.8%, respectively. Conclusion MR-DWIBS may be clinically useful to visually detect mediastinal lymph nodes and ADC measurement can aid in malignant node discrimination. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.
Zhang T.,Shandong University |
Rong N.,Shandong Provincial Chest Hospital |
Chen J.,Jinan Seventh Peoples Hospital |
Zou C.,Shandong University |
And 3 more authors.
PLoS ONE | Year: 2013
Aim: The role of Sirtuin 1 (SIRT 1) in carcinogenesis is controversial. This study was to explore the association between the SIRT1 expression and the clinical characteristics, the responsiveness to chemotherapy and prognosis in Non-small cell lung cancer (NSCLC). Methods: We enrolled 295 patients with inoperable advanced stage of NSCLC, namely, stage III (A+B) and IV NSCLC. All patients had received platinum-based chemotherapy after diagnosis and the chemotherapy response were evaluated. All patients were followed up for overall survival (OS) and progression free survival (PFS). In vitro, H292 cells were tranfected with SIRT1 small interfering RNA (siRNA). The cell biological behaviors and chemosensitivity to cisplatin treatment were studied. The in vivo tumorgenesis and metastasis assays were performed in nude mice. Results: We found that the SIRT1 expressions were significantly associated with the tumor stage, tumor size and differentiation status. Patients with high SIRT 1 expressions had a significantly higher chance to be resistant to chemotherapy than those with low SIRT 1 expression. Patients with high expression of SIRT1 had significantly shorter OS and DFS than those with low expression. Cox analyses confirmed that the SIRT 1 expression was a strong predictor for a poor OS and PFS in NSCLC patients underwent Platinum-based chemotherapy. In vitro studies revealed that the reduced expression SIRT 1 by siRNA technique significantly inhibited cell proliferation, migration and invasion. More importantly, SIRT1 si-RNA significantly enhanced the chemosensitivity of H292 cells to cisplatin treatment. The in vivo tumorgenesis and metastasis assays showed that SIRT1 knockdown dramatically reduced the tumor volume and the metastatic ability in nude mice. Conclusion: Collectively, our data suggest that the SIRT1 expression may be a molecular marker associated with the NSLCLC clinical features, treatment responsiveness and prognosis of advanced NSCLC. Copyright: © 2013 Zhang et al.
Ren M.-Y.,Shandong Provincial Chest Hospital |
Sui S.-J.,Shandong University
Molecular Biology Reports | Year: 2012
The pathophysiological basis of heart failure is cardiac remodeling, a process that comprises structural and functional changes including cardiomyocyte proliferation, hypertrophy, necrosis, apoptosis, autophagy, interstitial fibrosis, contractile dysfunction and ventricular dilatation. Accumulating evidence demonstrate that tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is involved in the process by binding its receptor fibroblast growth factor-inducible molecule 14 (Fn14). In this review, we will discuss the potential role of the TWEAK/Fn14 axis in cardiac remodeling, elucidate its possible mechanisms and explore new therapeutic targets for heart failure. © Springer Science+Business Media B.V. 2012.
Song J.t.,Shandong University |
Hu B.,Shandong University |
Qu H.y.,Shandong University |
Qu H.y.,Shandong Provincial Chest Hospital |
And 3 more authors.
PLoS ONE | Year: 2012
Objectives: Stretch affects vascular smooth muscle cell proliferation and apoptosis, and several responsible genes have been proposed. We tested whether the expression of microRNA 21 (miR-21) is modulated by stretch and is involved in stretch-induced proliferation and apoptosis of human aortic smooth muscle cells (HASMCs). Methods and Results: RT-PCR revealed that elevated stretch (16% elongation, 1 Hz) increased miR-21 expression in cultured HASMCs, and moderate stretch (10% elongation, 1 Hz) decreased the expression. BrdU incorporation assay and cell counting showed miR-21 involved in the proliferation of HASMCs mediated by stretch, likely by regulating the expression of p27 and phosphorylated retinoblastoma protein (p-Rb). FACS analysis revealed that the complex of miR-21 and programmed cell death protein 4 (PDCD4) participated in regulating apoptosis with stretch. Stretch increased the expression of primary miR-21 and pre-miR-21 in HASMCs. Electrophoretic mobility shift assay (EMSA) demonstrated that stretch increased NF-κB and AP-1 activities in HASMCs, and blockade of AP-1 activity by c-jun siRNA significantly suppressed stretch-induced miR-21 expression. Conclusions: Cyclic stretch modulates miR-21 expression in cultured HASMCs, and miR-21 plays important roles in regulating proliferation and apoptosis mediated by stretch. Stretch upregulates miR-21 expression at least in part at the transcription level and AP-1 is essential for stretch-induced miR-21 expression. © 2012 Song et al.
Zhao T.-T.,Shandong Provincial Chest Hospital |
Chen B.,Shandong University |
Wang H.-P.,Shandong University |
Wang R.,Shandong University |
Zhang H.,Shandong University
Clinical Biochemistry | Year: 2013
Objective: The aim of the study is to evaluate the distribution and correlation of toxic and essential elements in whole blood from 0- to 6-year-old children from Jinan, China. Design and methods: Levels of toxic and essential elements found in the whole blood of 1110 children were analyzed using an atomic absorption spectrometer. Distributions and correlations of trace elements in different age groups were analyzed and compared. Results: The overall mean blood lead (Pb) and cadmium (Cd) levels (49.42. ±. 20.16 and 1.47. ±. 1.08. μg/L, respectively) were relatively stable among different age groups. The prevalence of Pb and Cd intoxication in all children was 1.4% and 0.4%, respectively. Calcium (Ca) levels decreased gradually with age, with an overall concentration of 1.82. ±. 0.15. mmol/L. Whole levels of copper (Cu) and magnesium (Mg) were 17.79. ±. 4.22. μmol/L and 1.52. ±. 0.14. mmol/L, respectively. While 7.6% of all children showed Cu levels below the normal threshold, the levels of Mg were stable in different age groups. Though the overall mean blood zinc (Zn) and iron (Fe) concentrations (59.59. ±. 15.15. μmol/L and 7.39. ±. 0.74. mmol/L, respectively) gradually increased with age and the overall deficiency levels (59.8% and 59.5%, respectively) decreased with age, Zn and Fe deficiencies were still very common. Significant positive correlations were found when comparing Cu to Zn, Ca, Mg, and Fe; Zn to Mg and Fe; and Mg to Ca and Fe. Additionally, minor positive correlations were found when comparing Pb to Zn and Fe. Conclusion: The degree of Pb and Cd intoxication in all the children studied was low; however, Pb and Cd exposure was still evident. The importance of Ca deficiency and supplementation is well recognized, but the severity of Fe and Zn deficiency is not as well documented. © 2013 The Canadian Society of Clinical Chemists.
Zhan Y.,Shandong University |
Zhan Y.,Shandong Provincial Chest Hospital |
Jiang L.,Shandong University
Experimental and Therapeutic Medicine | Year: 2015
Pulmonary tuberculosis (PTB) is a high burden infectious disease in China. The immune function is damaged in patients with diabetes mellitus (DM) who are easy to infect with Mycobacterium tuberculosis (Mtb). The growth of Mtb has been shown to be restrained following the administration of vitamin D and antimicrobial peptide cathelicidin (LL‑37); however, the effect in patients with DM and PTB remains unclear. Vitamin D can regulate the immune system through Vitamin D receptors expressed in T helper (Th) cells. The aim of the present study was to analyze the status and correlations of vitamin D, LL‑37 and Th‑associated cytokines in patients with PTB or PTB with DM (DMPTB). Serum 25‑hydroxyvitamin D3 [25(OH)D3] levels were measured by liquid chromatography‑tandem mass spectrometry, while plasma LL-37 levels were analyzed using a solid‑phase enzyme‑linked immunosorbent assay. Flow cytometry was used to analyze the levels of Th cytokines, including Th1‑associated IFN‑γ, Th2‑associated IL‑4 and Th17‑associated IL‑17. The results revealed that patients with PTB and DMPTB were vitamin D deficient or had insufficient vitamin D levels. Furthermore, the levels of LL‑37, IFN‑γ, IL‑4 and IL‑17 were higher in the PTB and DMPTB groups when compared with the normal controls. These results indicated that vitamin D supplementation is necessary for PTB and DMPTB patients. In addition, LL‑37, IFN‑γ and IL‑17 may be diagnostic indexes that become elevated in the compensatory response caused by Mtb infection. Vitamin D can regulate the immune status in patients suffering from PTB. © 2015, Spandidos Publications. All rights reserved.
Deng Y.F.,Shandong Provincial Chest Hospital
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2010
To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong. Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units (MIRU) methods. 12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns, and 481 (86.2%) strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level, comparing to the susceptible strains. Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.
Zhu C.,Tianjin Medical University |
Zhu C.,Shandong Provincial Chest Hospital |
Cao R.,Tianjin Medical University |
Zhang S.-X.,Tianjin Medical University |
And 2 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013
Some sulphated polysaccharides can bind bFGF but are unable to present bFGF to its high-affinity receptors. Fucoidan, a sulphated polysaccharide purified from brown algae, which has been used as an anticancer drug in traditional Chinese medicine for hundreds of years, exhibits a variety of anticancer effects, including the induction of the apoptosis and autophagy of cancer cells, the inhibition of the growth of cancer cells, the induction of angiogenesis, and the improvement of antitumour immunity. Our research shows that fucoidan dose not inhibit the expressions of VEGF, bFGF, IL-8, and heparanase in HCC cells and/or tumour tissues. Moreover, fucoidan exhibited low affinity for bFGF and could not block the binding of bFGF to heparan sulphated. Although fucoidan had no effect on angiogenesis and apoptosis in vivo, this drug significantly inhibited the tumour growth and the expression of PCNA. These results suggest that fucoidan exhibits an anticancer effect in vivo at least partly through inhibition of the proliferation of HCC cells, although it is unable to suppress the angiogenesis induced by HCC. © 2013 Cong Zhu et al.