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Zhao D.,University of Jinan | Yu Y.,Shandong Product Quality Inspection Research Institute | Xu C.,University of Jinan
RSC Advances | Year: 2015

A sensitive electrochemical immunosensor for the detection of human chorionic gonadotropin (hCG) is designed based on a novel signal amplification strategy. A hierarchical nanoporous (HNP) AuAg alloy with the advantages of a large surface area, excellent structure stability, and rich pore channels is used as an hCG antibody carrier for the preparation of a highly sensitive immunosensor. The HNP-AuAg alloy with bimodal ligament/pore size distribution is fabricated by means of simple dealloying and redealloying of the AuAgAl source alloy combined with an annealing operation. Graphene sheets (GS) and ionic liquid (IL) composites have been introduced as transducing materials to modify the conductivity as well promote the electron transfer in the immunosensor. Based on the dual-amplification effects of the HNP-AuAg alloy and the IL/GS composite film, the constructed immunosensor exhibits an enhanced performance for hCG detection compared with that based on single nanoporous (NP) Au, including a lower detection limit of 0.01 ng mL-1 and wider linear range from 0.05 to 35.0 ng mL-1. Moreover, the immunosensor exhibits respectable reproducibility and stability, indicating a potential application in clinical monitoring of hCG. 2016 The Royal Society of Chemistry.


Shang Z.X.,Shandong Product Quality Inspection Research Institute | Wang Y.,Shandong External Science and Technology Exchange Center | Zhang R.,Shandong Institute of Medicine and Health Information
Advanced Materials Research | Year: 2014

In this paper, the economic planning was conducted for the application of game theory in multi-objective area. The study and combined application of fuzzy comprehensive evaluation and analytical hierarchy process were also performed. On this basis, a portfolio selection model based on fuzzy comprehensive evaluation was designed and its application was simulated. © (2014) Trans Tech Publications, Switzerland.


Lu B.,Shandong University | Xu Y.,Shandong University | Xin F.,Shandong Product Quality Inspection Research Institute | Xiao G.,Shandong University
2014 17th International Conference on Electrical Machines and Systems, ICEMS 2014 | Year: 2015

Compared with the Tooth Layer Specific Permeability (TLSP) method being mainly adopted so far to calculate the electromagnetic torque of stepping motors, a new method is proposed in this paper, which is called the Tooth Layer Calculated Torque (TLCT) method. The concept of TLCT is defined firstly and its correctness when used to calculate the electromagnetic torque is proved theoretically at the same time. Then how to use the TLCT method in calculating the electromagnetic torque in stepping motors is described. At last, a comparative research among the TLSP method, the TLCT one and the finite element one (FEM) is performed, with the fact that, the TLCT method features simplified calculation and high accuracy compared with the former method. © 2014 IEEE.


Zhang N.,Nanjing Agricultural University | He X.,Nanjing Agricultural University | Zhang J.,Shandong Product Quality Inspection Research Institute | Raza W.,Nanjing Agricultural University | And 4 more authors.
Pedosphere | Year: 2014

Fusarium wilt is one of the most serious diseases of banana plants caused by soil-borne pathogen Fusarium oxysporum f.sp. cubense (FOC). In this study a pot experiment was conducted to evaluate the effects of different bio-organic fertilizers (BIOs) on Fusarium wilt of banana, including the investigations of disease incidence, chitinase and β-1,3-glucanase activities of banana plants, and FOC populations as well as soil rhizosphere microbial community. Five fertilization treatments were considered, including chemical fertilizer containing the same N, P and K concentrations as the BIO (control), and matured compost mixed with antagonists Paenibacillus polymyxa SQR-21 and Trichoderma harzianum T37 (BIO1), Bacillus amyloliquefaciens N6 (BIO2), Bacillus subtilis N11 (BIO3), and the combination of N6 and N11 (BIO4). The results indicated that the application of BIOs significantly decreased the incidence rate of Fusarium wilt by up to 80% compared with the control. BIOs also significantly promoted plant growth, and increased chitinase and β-1,3-glucanase activities by 55%-65% and 17.3%-120.1%, respectively, in the banana roots. The population of FOC in the rhizosphere soil was decreased significantly to about 104 colony forming units g-1 with treatment of BIOs. Serial dilution plating and denaturing gradient gel electrophoresis analysis revealed that the application of BIOs increased the densities of bacteria and actinomycetes but decreased the number of fungi in the rhizosphere soil. In general, the application of BIOs revealed a great potential for the control of Fusarium wilt disease of banana plants. © 2014 Soil Science Society of China.


Wang W.,Shandong University | Wang Z.,Shandong University | Cheng B.,Shandong University | Zhang J.,Shandong Product Quality Inspection Research Institute | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2014

Using native signal peptide, an alkaliphilic actinomycete xylanase XynK was overexpressed in Escherichia coli and secreted into the culture medium completely. At its optimum catalytic temperature of 55 °C, the cellulose-free xylanase exhibits high activity and stability at pH 7.0-11.0. In comparison with the well-studied actinomycete xylanase from Streptomyces lividans, as an alkaliphilic xylanase, XynK exhibited different biochemical and catalytic characteristics. With the aid of site-directed mutagenesis, some residues were demonstrated to be important to the activity, stability, or substrate binding of the enzyme. The pH stability of mutants H131S and W135A both decreased obviously under high pH values. Combined with their K m parameters and homology model analysis, His131 was proposed to be important to both substrate binding and enzyme catalyzing, whereas Trp135 significantly influenced enzyme stability. Good stability under alkaline condition, as well as high secretory expression implies good potentials of the alkaline xylanase in various industrial applications. In addition, results from site-directed mutagenesis provide useful information for further pH stability mechanisms investigation. © 2014 Springer Science+Business Media Dordrecht.

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