Han H.X.,Shandong Academy of Agricultural Sciences |
Han H.X.,Shandong Poultry Breeding and Engineering Technology Research Center |
Li H.M.,Shandong Academy of Agricultural Sciences |
Cao D.G.,Shandong Academy of Agricultural Sciences |
And 13 more authors.
Journal of Food, Agriculture and Environment | Year: 2015
We have assessed the relationships between immune trait (antibody titers against sheep red blood cell, SRBC; avian influenza virus, AIV; Newcastle disease virus, NDV) and varieties of MHC B-LBII Gene in chicken breeds (Lu-qin chicken, Shi qi-za chicken, Lang-ya chicken) of Shandong Province. We selected 300 chickens randomly from the three indigenous chicken populations. The variations of MHC B-L BII gene were detected by directly DNA sequencing and polymerase chain reaction–single strand conformation polymorphism (PCR-SSCP). The results demonstrated that there were about 17∼ 24 nucleotide mutations in these breeds and could affect 13∼ 19 amino acid sequences. Another results indicated that there was significant correlation between 7∼ 8 SNPs of the MHC B-LBII region and some immune traits (P<0.05 or P<0.01). Locus T97A sites and T138 A were associated with immune traits against Newcastle disease virus and H9 genotype of avian influenza virus, respectively, in Lu-qin and Lang-ya chicken. Genetically, these two breeds have the closest similarity among these three breeds, where gene mutation and antibody titer associated with immune traits were basically the same. Locus T97A loci in three species variation in both and with sheep red blood cell antibodies, Newcastle disease and avian influenza antibody titers were significantly associated, as with the three immune traits were significantly associated molecular markers. All those results suggest relationships among the different varieties of MHC B-LBII and immune traits in the three local breeds. © 2015, World Food Ltd. and WFL Publishers. All rights reserved.