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Pang X.,University of Jinan | Zhang Y.,University of Jinan | Liu C.,University of Jinan | Huang Y.,University of Jinan | And 4 more authors.
Journal of Materials Chemistry B | Year: 2016

Herein, we demonstrated the protocol of a visible light induced photoelectrochemical cytoassay on the basis of TiO2 nanorods/carboxylated g-C3N4/ox-graphene quantum dots coupling with a biospecific interaction for the ultrasensitive detection of fibroblast-like synoviocyte cells. The nanohybridization assembly of TiO2 NRs, carboxylated g-C3N4 and ox-GQDs showed good PEC performance through the excellent electrical conductivity as well as the high photon-to-electron conversion efficiency and improved the sensitivity of the cytosensor. To perform the cytoassay, CD95 antibody, a marker of FLS cells, was conjugated onto the TiO2 NR/carboxylated g-C3N4/ox-GQD modified electrode to trap the cell target by using EDC/NHS coupling reactions. The concentrations of FLS cells were determined by the decrease in photocurrent intensity resulting from the increase in steric hindrance from FLS cells. This strategy opens a simple perspective for the application of carboxylated g-C3N4 and ox-GQDs as light-harvesting architectures. © The Royal Society of Chemistry 2106. Source


Cai W.-W.,Shandong Medicinal Biotechnology Center
Chinese Journal of Biologicals | Year: 2013

Objective: To investigate the effect of various dosages of Antarctic krill powder, alone or in combination with calcium, on osteoporosis in rats. Methods: One hundred of eight week old rats were divided into eight test groups as well as one sham operation control group and one model control group, 10 for each. Osteoporosis model was established by ovariectomy, while the partial fatty tissues adjacent to ovaries of rats in sham operation control group were resected. The rats in OVX + LK, OVX + MK and OVX + HK groups were treated with Antarctic krill powder at low, moderate and high dosages, while those in OVX + Ca group with calcium, those in OVX + LK + Ca, OVX + MK + Ca and OVX + HK + Ca groups with Antarctic krill powder at low, moderate and high dosages + calcium, and those in NaF group with sodium fluoride, by gavage, respectively. However, the rats in sham operation control (Sham) and model control (OVX) groups were fed with normal forage. Twelve weeks later, the serum samples were collected and determined for calcium ion concentration and alkaline phosphatase (ALP) activity. The thigh-bones of rats were collected, observed for differences in bone mineral density and bone maximum load, and determined for ash content, while the shin bone were determined for fluorine content. Results: The serum calcium contents of rats decreased gradually with the increasing dosage of Antarctic krill powder, which showed significant difference in OVX + HK and OVX groups(P < 0.05), while were higher in OVX + LK + Ca, OVX + MK + Ca and OVX + HK + Ca groups than in OVX group. The ALP activity increased with the increasing dosage of Antarctic krill powder, which were higher in OVX + LK, OVX + MK and OVX + HK groups than in OVX group (P < 0.05). The bone density of rats in OVX + LK + Ca, OVX + MK + Ca and OVX + HK + Ca groups were higher than those in OVX, OVX + LK, OVX + MK and OVX + HK groups. The bone maximum loads of rats in OVX + LK, OVX + MK + Ca and OVX + HK + Ca groups were significantly higher than those in OVX group (each P < 0.05). As compared with those in OVX group, the fluorine contents in bones of rats in OVX + LK, OVX + MK and OVX + HK groups increased with the dosage of Antarctic krill powder, while decreased significantly in OVX + LK + Ca, OVX + MK + Ca and OVX + HK + Ca groups (each P < 0.01). The fluorine contents in OVX + LK + Ca, OVX + MK + Ca and OVX + HK + Ca groups were lower than those in OVX + LK, OVX + MK and OVX + HK groups, respectively. The ash content in OVX + LK, OVX + MK and OVX + HK groups were significantly higher than those in OVX group (P > 0.05). Conclusion: The Antarctic krill powder at a low dosage showed satisfactory curative effect on osteoporosis. However, the curative effect of Antarctic krill powder at a moderate dosage was satisfactory when its was administered in combination with calcium. Source


Pang X.,University of Jinan | Zhang Y.,University of Jinan | Pan J.,Shandong Medicinal Biotechnology Center | Zhao Y.,Shandong Academy of Sciences | And 5 more authors.
Biosensors and Bioelectronics | Year: 2016

Based on ZnO nanorods (NRs)/CH3NH3PbI3/nitrogen-doped carbon quantum dots (NCQDs) nanocomposites, the highly sensitive detection of fibroblast-like synoviocyte (FLS) cell was realized by a photoelectrochemical (PEC) biosensor. ZnO/CH3NH3PbI3/NCQDs nanocomposites were exploited as the photo-to-electron generator to produce the signal. CH3NH3PbI3 was spin-coated on ZnO surface after ZnO NRs grew on ITO electrode then by dropping on the modified electrode, NCQDs were diffused and adhered to the surface of ZnO and CH3NH3PbI3. In the presence of EDC/NHS, the combination of CH3NH3PbI3 and NCQDs was achieved by the carboxyl groups (-COOH) and amino groups (-NH2) in the preparation process. Furthermore, the capture probe of FLS cell, CD95 antibody, can be anchored by -COOH and -NH2 groups through EDC/NHS. The specific recognition between the antibody capture probes and cell targets gained high-sensitive detection for FLS cell for the first time. The developed biosensor showed a wide linear range from 1.0×104cell/mL to 10cell/mL and a low detection limit of 2cell/mL. This kind of biosensor would provide a novel detection strategy for FLS cell. © 2015 Elsevier B.V. Source


Pang X.,University of Jinan | Pan J.,Shandong Medicinal Biotechnology Center | Gao P.,University of Jinan | Wang Y.,University of Jinan | And 3 more authors.
Biosensors and Bioelectronics | Year: 2015

It was reported that Proprotein convertase subtilisin/kexin type 6 (PCSK6) can promote the progression of rheumatoid arthritis to a higher aggressive status. In this work, a novel visible light induced photoelectrochemical (PEC) platform was designed to detect PCSK6 gene. ZnOatCdTe nanocable arrays/carboxylated g-C3N4 used as the PEC signal generator. Hexagonal ZnO nanorods grew on ITO electrode firstly. CdTe were then electrodeposited on the ZnO nanorods surface to enhance the photogenerated h+/e- separation efficiency. Carboxylated g-C3N4 was utilized to improve h+/e- separation efficiency and anchor the capture probes of PCSK6 gene by the covalent bonding effect. The 5' and 3' primers captured PCSK6 ssDNA by the specific recognition. The linear range was 10pg/mL to 20.0ng/mL with a detection limit of 2pg/mL. © 2015 Elsevier B.V. Source


Wu X.-Y.,Shandong Medicinal Biotechnology Center | Wang S.-L.,Shandong Medicinal Biotechnology Center
Chinese Journal of Biologicals | Year: 2011

Objective: To develop a novel method for harvest of hematopoietic stem cells and provide an experimental basis for clinical application. Methods: Gelatin sponges (GS) were implanted into the spatium intermusculare of mice hind limbs, in which the migrating cells were isolated 12 d later. The expressions of CD34 +, Sca-1 +, and CD34 +Sca-1 + cells in migrating cells (MCs) and bone marrow cells (BMCs) were determined by flow cytometry and IFA, and the density of hematopoietic colonies by colony-forming cell assay in vitro. Results: The count of migrating cells reached the maximum 12 d after transplantation. The proportions of CD34 +, Sca-1 + and CD34 +Sca-1 + cells (each P < 0.05) as well as the density of hematopoietic colonies were significantly higher in MCs than in BMCs (P < 0.01). Conclusion: An effective method for harvest of hematopoietic stem cells was successfully developed with GS as a biomaterial, by which autologous cell therapy might be feasible. Source

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