Time filter

Source Type

Wang J.,Shandong Marine Resource and Environment Research Institute | Zhang D.,Shandong Marine Resource and Environment Research Institute | Sun Y.,Shandong Marine Resource and Environment Research Institute | Wang S.,Shandong Marine Resource and Environment Research Institute | And 3 more authors.
Aquaculture Research | Year: 2016

An 8-week feeding trial was conducted to evaluate the effects of a dairy-yeast prebiotic (Grobiotic®-A) on growth performance, body composition, antioxidant capacity and immune functions of juvenile starry flounder, Platichtahys stellatus. Three triplicates of starry flounder (average initial weight of 15.05 ± 0.04 g) were fed one of six diets formulated to supplement dairy-yeast prebiotic at 0%, 0.4%, 0.8%, 1.2%, 1.6% or 2.0% respectively. Results showed that weight gain, daily feed intake, protein efficiency rate and condition factor increased significantly (P < 0.05) whereas feed conversion ratio decreased significantly with the increasing level of the dairy-yeast prebiotic supplement. Crude lipid content in dorsal muscle and liver decreased significantly (P < 0.05). No significant differences occurred in moisture and crude protein content of dorsal muscle and liver with the increasing level of the dairy-yeast prebiotic supplement. Compared with fish feed the basal diet, the activity of catalase and total antioxidant capability in serum and liver, as well as total superoxide dismutase in serum all significantly increased with high level of the prebiotic, while the malondialdehyde content in serum and liver decreased significantly. In serum, the activity of alkaline phosphatase, lysozyme, complement and the content of nitric oxide were significantly increased at higher level of prebiotic supplementation while no significant differences were found in total protein, albumin, globulin, albumin to globulin ratio, haemoglobin, acid phosphatase and myeloperoxidase. Based on weight gain response using the quadratic regression, combine the antioxidant and immune indices, the optimum dietary dairy-yeast prebiotic level for juvenile starry flounder was estimated to be 1.33% under these experimental conditions. © 2016 John Wiley & Sons Ltd.


PubMed | Shandong Marine Resource and Environment Research Institute, Freshwater Fisheries Research Institute of Linyi and Ocean University of China
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Octopus minor (Sasaki, 1920) is an economically important cephalopod that is found in the northern coastal waters of China. In this study, we investigated genetic differentiation in fishery populations using amplified fragment length polymorphisms (AFLPs). A total of 150 individuals were collected from five locations: Dalian (DL), Yan-tai (YT), Qingdao (QD), Lianyungang (LY), and Zhoushan (ZS), and 243 reproducible bands were amplified using five AFLP primer combinations. The percentage of polymorphic bands ranged from 53.33 to 76.08%. Neis genetic identity ranged from 0.9139 to 0.9713, and the genetic distance ranged from 0.0291 to 0.0900. A phylogenetic tree was constructed using the unweighted pair group method with arithmetic mean, based on the genetic distance. The DL and YT populations originated from one clade, while the QD, LY, and ZS populations originated from another. The results indicate that the O. minor stock consisted of two genetic populations with an overall significantly analogous FST value (0.1088, P < 0.05). Most of the variance was within populations. These findings will be important for more sustainable octopus fisheries, so that this marine resource can be conserved for its long-term utilization.


Deng Y.,Shandong University | Tong X.,Shandong University | Liang Z.,Shandong University | Zhao J.,Shandong Marine Resource and Environment Research Institute
Proceedings of the International Offshore and Polar Engineering Conference | Year: 2015

The dynamic mesh technique are used to simulate the circular cylinder motion. An Elastically supported cylinder placed in uniform flow and also in combination of uniform and oscillating flows at low Reynolds number values are simulated. The uniform and oscillating flows are both in the x direction. SIMPLE algorithm is used to solve the Navier-Stokes equations. In the paper, we compute the lift and oscillating motion amplitude as functions of Reynolds number and time at different mass ratios. Simulations show the vortex induced vibration process of an elastically supported circular cylinder in uniform flow and compare the oscillating motion amplitude of cylinder and lift coefficient in combination of uniform and oscillating flow at high and low mass ratio. Copyright © 2015 by the International Society of Offshore and Polar Engineers (ISOPE).


Xia B.,Shandong Marine Resource and Environment Research Institute | Wang J.,Shandong Marine Resource and Environment Research Institute | Gao Q.-F.,Ocean University of China | Sun Y.,Shandong Marine Resource and Environment Research Institute | And 3 more authors.
Aquaculture | Year: 2015

Stable isotope technologies can effectively yield valuable information on the feasibility of estimating incorporation of specific macro- and micro-nutrients, rather than ingestion, through straightforward procedures with high accuracy and rapid sample analysis. In the present study, sea cucumbers were fed five isonitrogenous and isoenergetic diets containing different ratios of Sargassum thunbergii to soybean meal (100:0, 75:25, 50:50, 25:75 and 0:100; in terms of supplied nitrogen content) for 3mo to assess the nutritional contributions of dietary protein sources. The results revealed that owing to the different enzyme systems and biochemical components of tissue and the discrepancy in dietary protein quality, nitrogen isotopic turnover rates exhibited obvious shifts between the tissues and between the experimental diets. Nitrogen half-lives reached 12.48-20.89d for intestine and 25.28-40.37d for body wall, respectively. The faster metabolism in intestine resulted in the higher metabolic contributions to nitrogen turnover (76-79%) than body wall (52-59%). The constant proportional contributions of growth and metabolism between the experimental diets indicated that the tissues kept stable bioenergetic distribution strategy in response to the fluctuation of dietary protein quality. The fractionations (δ15N) ranged from 2.07‰ to 3.26‰. Calculated by nitrogen isotopic mixing model and estimated δ15N by time-based turnover model, the biased nutritional contributions of dietary protein sources were observed, demonstrating higher dietary quality and preferential absorption of S. thunbergii for sea cucumber. © 2014.


Feng J.,Zhejiang University | Chen Y.,Zhejiang University | Liu X.,Shandong Marine Resource and Environment Research Institute | Liu S.,Zhejiang University
Food Chemistry | Year: 2014

Novel strategy of Gemini-like modification has been applied in development of new nonionic surfactants, tea saponin esters, with enhanced surface activity by simple esterification. Tea saponin was treated with acyl chlorides of different chain length and different ratio of tea saponin and acyl chloride under alkaline condition. The structures of tea saponin esters were analysed and confirmed by FT-IR, NMR and ESI-MS. Surface activity investigation revealed that esterification with the chain length of C12 and C14 and the ratio of 1:4 to 1:6 produced superior surface activity compared with tea saponin. The exceptional surface activity of the new surfactants suggested their great potential application in food industry as green surfactants due to their environmental benign nature as well as simple and inexpensive preparation. The strategy of Gemini-like modification will facilitate development of green surfactants based on natural resources. © 2014 Elsevier Ltd. All rights reserved.


Deng Y.,Shandong University | Tong X.,Shandong University | Liang Z.,Shandong University | Zhao J.,Shandong Marine Resource and Environment Research Institute
Proceedings of the International Offshore and Polar Engineering Conference | Year: 2016

This paper studies on the vortex-induced vibration of a cylinder in combination of uniform and oscillating flows. The dynamic mesh technique are used to simulate the circular cylinder motion. An elastically supported cylinder placed in uniform flow and also in combination of uniform and oscillating flows are simulated. The uniform and oscillating flows are both in the x direction. SIMPLE algorithm is used to solve the Navier-Stokes equations. In the paper, we compute the lift and oscillating motion amplitude as functions of Reynolds number and time at different mass ratios. Simulations show the vortex-induced vibration process of an elastically supported circular cylinder in uniform flow. We compare the oscillating motion amplitude of cylinder and lift coefficient in combined flows at different mass and damping ratios. The lock-in region of the cylinder vibration system with low mass ratio is wider than that of high mass ratio. The amplitude of oscillating cylinder decreases when damping ratio increases. © Copyright 2016 by the International Society of Offshore and Polar Engineers (ISOPE).


Wei X.,Shandong Marine Resource and Environment Research Institute | Xu J.,Shandong Marine Resource and Environment Research Institute | Yang J.,Shandong Marine Resource and Environment Research Institute | Liu X.,Shandong Marine Resource and Environment Research Institute | And 3 more authors.
Fish and Shellfish Immunology | Year: 2015

Serpin is an important member of serine protease inhibitors (SPIs), which is capable of regulating proteolytic events and involving in a variety of physiological processes. In present study, a Serpin homologwas identified from Octopus ocellatus (designated as OoSerpin). Full-length cDNA of OoSerpin was of 1735bp, containing a 5' untranslated region of 214bp, a 3' UTR of 282bp, and an open reading frame of 1239bp. The open reading frame encoded a polypeptide of 412 amino acids which has a predicted molecular weight of 46.5kDa and an isoelectric point of 8.52. The OoSerpin protein shares 37% sequence identity with other Serpins from Mus musculus (NP_941373) and Ixodes scapularis (XP_002407493). The existence of a conserved SERPIN domain strongly suggested that OoSerpin was a member of the Serpin subfamily. Expression patterns of OoSerpin, both in tissues and towards bacterial stimulation, were then characterized. The mRNA of OoSerpin was constitutively expressed at different levels in all tested tissues of untreated O. ocellatus, including mantle (lowest), muscle, renal sac, gill, hemocyte, gonad, systemic heart, and hepatopancreas (highest). The transcriptional level of OoSerpin was significantly up-regulated (P<0.01) in O. ocellatus upon bacterial challenges with Vibrio anguillarum and Micrococcus luteus, indicating its involvement in the antibacterial immune response. Furthermore, rOoSerpin, the recombinant protein of OoSerpin, exhibited strong abilities to inhibit proteinase activities of trypsin and chymotrypsin as well as the growth of Escherichia coli. Our results demonstrate that OoSerpin is a potential antibacterial factor involved in the immune response of O. ocellatus against bacterial infection. © 2014 Elsevier Ltd.


PubMed | Shandong Marine Resource and Environment Research Institute
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.


PubMed | CAS Yantai Institute of Coastal Zone Research and Shandong Marine Resource and Environment Research Institute
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2015

C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other C-type lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca(2+)-binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different up-regulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca(2+)-dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.


PubMed | CAS Yantai Institute of Coastal Zone Research and Shandong Marine Resource and Environment Research Institute
Type: | Journal: The Science of the total environment | Year: 2016

A verified method for measuring Semicarbazide (SEM) in seawater, sediments, and shellfish was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 30 stations were radially distributed in Jincheng and Sishili Bays in the Bohai and Yellow Seas, and 1025 monitoring data were collected in 41 voyages, 615 seawater samples, 320 sediment samples and 90 shellfish samples. The concentration ranged from 0.011g/L to 0.093g/L and 0 to 0.75g/kg in seawater and shellfish respectively, but SEM in sediment was all below the limit of detection. Temporal and spatial distribution of SEM was investigated using multivariate analysis to estimate the degree of SEM pollution. Based on the SEM concentration in the three sample types, together with our previous findings, early warning values were deduced for SEM in seawater, and the developed method overcame shortcomings with existing technologies. The results may be helpful to draft national baseline values for SEM in seawater and sediments, and provide a scientific basis for assessing the impacts of SEM on marine ecology and human health.

Loading Shandong Marine Resource and Environment Research Institute collaborators
Loading Shandong Marine Resource and Environment Research Institute collaborators