Shandong Marine Resource and Environment Research Institute

Yantai, China

Shandong Marine Resource and Environment Research Institute

Yantai, China
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Xia B.,Shandong Marine Resource and Environment Research Institute | Xia B.,Qingdao Agricultural University | Ren Y.,Qingdao Agricultural University | Wang J.,Shandong Marine Resource and Environment Research Institute | And 2 more authors.
Aquaculture | Year: 2017

Feeding frequency and density are two of the most important factors that directly affect the growth and physiology of sea cucumber Apostichopus japonicus. In the present study, a 60-day experiment was conducted to evaluate the effects of feeding frequency (1 time, 2 times and 3 times per day) and stocking density (D10, D20, D40 and D60) on growth performance, feed utilization, endocrine response and energy budget of sea cucumber. The results revealed that there was no significant interaction between feeding frequency and stocking density on final weight and specific growth rate (SGR). The maximum SGR occurred at treatment of stocking density D10 with feeding thrice per day. Stepwise multiple regression analysis showed that SGRW and SGRE increased with increasing feeding frequency, and decreased with the increase of stocking density. Density had significant influence on coefficient of variation (CV) for the sea cucumber body weight. For D10 and D20, no significant difference in CV was found between different feeding frequencies. Feed intake, energy intake and feces production rate were significantly affected by feeding frequency, stocking density and their interaction. Density also had significant influence on food conversion efficiency and protein efficiency ratio. Apparent digestibility coefficients of crude protein and crude lipid exhibited remarkable descending trends as stocking density increased. The energy for growth decreased and energy required for metabolism increased with the increase of stocking density, suggesting that crowding stress modified their energy allocation by inhibiting the accumulation of growth energy and accelerating energy consumption of sea cucumber. Cortisol level in coelomic fluid of sea cucumber significantly increased with the increase of stocking density, which could generate energy to satisfy the increasing demand of stress-related energy. Lactate level significantly elevated, and glucose level in coelomic fluid and glycogen in muscle showed remarkable descending trends from D10 to D60, implying long-term energy consumption under high stocking density. Feeding frequency had significant influence on glucose, glycogen and cortisol levels. To some extent, increasing feeding frequency could effectively reduce coefficient of variation of sea cucumber, especially under high stocking density. Statement of relevance • In the present study, a 60-day experiment was conducted to evaluate the effects of feeding frequency (1 time, 2 times and 3 times per day) and stocking density (D10, D20, D40 and D60) on growth performance, feed utilization, endocrine response and energy budget of sea cucumber.• Increasing feeding frequency could effectively reduce coefficient of variation of sea cucumber.• The present study provided valuable information for the intensive culture of A. japonicus. © 2016 Elsevier B.V.


PubMed | Shandong Marine Resource and Environment Research Institute, Freshwater Fisheries Research Institute of Linyi and Ocean University of China
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Octopus minor (Sasaki, 1920) is an economically important cephalopod that is found in the northern coastal waters of China. In this study, we investigated genetic differentiation in fishery populations using amplified fragment length polymorphisms (AFLPs). A total of 150 individuals were collected from five locations: Dalian (DL), Yan-tai (YT), Qingdao (QD), Lianyungang (LY), and Zhoushan (ZS), and 243 reproducible bands were amplified using five AFLP primer combinations. The percentage of polymorphic bands ranged from 53.33 to 76.08%. Neis genetic identity ranged from 0.9139 to 0.9713, and the genetic distance ranged from 0.0291 to 0.0900. A phylogenetic tree was constructed using the unweighted pair group method with arithmetic mean, based on the genetic distance. The DL and YT populations originated from one clade, while the QD, LY, and ZS populations originated from another. The results indicate that the O. minor stock consisted of two genetic populations with an overall significantly analogous FST value (0.1088, P < 0.05). Most of the variance was within populations. These findings will be important for more sustainable octopus fisheries, so that this marine resource can be conserved for its long-term utilization.


Deng Y.,Shandong University | Tong X.,Shandong University | Liang Z.,Shandong University | Zhao J.,Shandong Marine Resource and Environment Research Institute
Proceedings of the International Offshore and Polar Engineering Conference | Year: 2015

The dynamic mesh technique are used to simulate the circular cylinder motion. An Elastically supported cylinder placed in uniform flow and also in combination of uniform and oscillating flows at low Reynolds number values are simulated. The uniform and oscillating flows are both in the x direction. SIMPLE algorithm is used to solve the Navier-Stokes equations. In the paper, we compute the lift and oscillating motion amplitude as functions of Reynolds number and time at different mass ratios. Simulations show the vortex induced vibration process of an elastically supported circular cylinder in uniform flow and compare the oscillating motion amplitude of cylinder and lift coefficient in combination of uniform and oscillating flow at high and low mass ratio. Copyright © 2015 by the International Society of Offshore and Polar Engineers (ISOPE).


Xia B.,Shandong Marine Resource and Environment Research Institute | Wang J.,Shandong Marine Resource and Environment Research Institute | Gao Q.-F.,Ocean University of China | Sun Y.,Shandong Marine Resource and Environment Research Institute | And 3 more authors.
Aquaculture | Year: 2015

Stable isotope technologies can effectively yield valuable information on the feasibility of estimating incorporation of specific macro- and micro-nutrients, rather than ingestion, through straightforward procedures with high accuracy and rapid sample analysis. In the present study, sea cucumbers were fed five isonitrogenous and isoenergetic diets containing different ratios of Sargassum thunbergii to soybean meal (100:0, 75:25, 50:50, 25:75 and 0:100; in terms of supplied nitrogen content) for 3mo to assess the nutritional contributions of dietary protein sources. The results revealed that owing to the different enzyme systems and biochemical components of tissue and the discrepancy in dietary protein quality, nitrogen isotopic turnover rates exhibited obvious shifts between the tissues and between the experimental diets. Nitrogen half-lives reached 12.48-20.89d for intestine and 25.28-40.37d for body wall, respectively. The faster metabolism in intestine resulted in the higher metabolic contributions to nitrogen turnover (76-79%) than body wall (52-59%). The constant proportional contributions of growth and metabolism between the experimental diets indicated that the tissues kept stable bioenergetic distribution strategy in response to the fluctuation of dietary protein quality. The fractionations (δ15N) ranged from 2.07‰ to 3.26‰. Calculated by nitrogen isotopic mixing model and estimated δ15N by time-based turnover model, the biased nutritional contributions of dietary protein sources were observed, demonstrating higher dietary quality and preferential absorption of S. thunbergii for sea cucumber. © 2014.


Feng J.,Zhejiang University | Chen Y.,Zhejiang University | Liu X.,Shandong Marine Resource and Environment Research Institute | Liu S.,Zhejiang University
Food Chemistry | Year: 2014

Novel strategy of Gemini-like modification has been applied in development of new nonionic surfactants, tea saponin esters, with enhanced surface activity by simple esterification. Tea saponin was treated with acyl chlorides of different chain length and different ratio of tea saponin and acyl chloride under alkaline condition. The structures of tea saponin esters were analysed and confirmed by FT-IR, NMR and ESI-MS. Surface activity investigation revealed that esterification with the chain length of C12 and C14 and the ratio of 1:4 to 1:6 produced superior surface activity compared with tea saponin. The exceptional surface activity of the new surfactants suggested their great potential application in food industry as green surfactants due to their environmental benign nature as well as simple and inexpensive preparation. The strategy of Gemini-like modification will facilitate development of green surfactants based on natural resources. © 2014 Elsevier Ltd. All rights reserved.


Deng Y.,Shandong University | Tong X.,Shandong University | Liang Z.,Shandong University | Zhao J.,Shandong Marine Resource and Environment Research Institute
Proceedings of the International Offshore and Polar Engineering Conference | Year: 2016

This paper studies on the vortex-induced vibration of a cylinder in combination of uniform and oscillating flows. The dynamic mesh technique are used to simulate the circular cylinder motion. An elastically supported cylinder placed in uniform flow and also in combination of uniform and oscillating flows are simulated. The uniform and oscillating flows are both in the x direction. SIMPLE algorithm is used to solve the Navier-Stokes equations. In the paper, we compute the lift and oscillating motion amplitude as functions of Reynolds number and time at different mass ratios. Simulations show the vortex-induced vibration process of an elastically supported circular cylinder in uniform flow. We compare the oscillating motion amplitude of cylinder and lift coefficient in combined flows at different mass and damping ratios. The lock-in region of the cylinder vibration system with low mass ratio is wider than that of high mass ratio. The amplitude of oscillating cylinder decreases when damping ratio increases. © Copyright 2016 by the International Society of Offshore and Polar Engineers (ISOPE).


Wei X.,Shandong Marine Resource and Environment Research Institute | Xu J.,Shandong Marine Resource and Environment Research Institute | Yang J.,Shandong Marine Resource and Environment Research Institute | Liu X.,Shandong Marine Resource and Environment Research Institute | And 3 more authors.
Fish and Shellfish Immunology | Year: 2015

Serpin is an important member of serine protease inhibitors (SPIs), which is capable of regulating proteolytic events and involving in a variety of physiological processes. In present study, a Serpin homologwas identified from Octopus ocellatus (designated as OoSerpin). Full-length cDNA of OoSerpin was of 1735bp, containing a 5' untranslated region of 214bp, a 3' UTR of 282bp, and an open reading frame of 1239bp. The open reading frame encoded a polypeptide of 412 amino acids which has a predicted molecular weight of 46.5kDa and an isoelectric point of 8.52. The OoSerpin protein shares 37% sequence identity with other Serpins from Mus musculus (NP_941373) and Ixodes scapularis (XP_002407493). The existence of a conserved SERPIN domain strongly suggested that OoSerpin was a member of the Serpin subfamily. Expression patterns of OoSerpin, both in tissues and towards bacterial stimulation, were then characterized. The mRNA of OoSerpin was constitutively expressed at different levels in all tested tissues of untreated O. ocellatus, including mantle (lowest), muscle, renal sac, gill, hemocyte, gonad, systemic heart, and hepatopancreas (highest). The transcriptional level of OoSerpin was significantly up-regulated (P<0.01) in O. ocellatus upon bacterial challenges with Vibrio anguillarum and Micrococcus luteus, indicating its involvement in the antibacterial immune response. Furthermore, rOoSerpin, the recombinant protein of OoSerpin, exhibited strong abilities to inhibit proteinase activities of trypsin and chymotrypsin as well as the growth of Escherichia coli. Our results demonstrate that OoSerpin is a potential antibacterial factor involved in the immune response of O. ocellatus against bacterial infection. © 2014 Elsevier Ltd.


PubMed | Shandong Marine Resource and Environment Research Institute
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.


PubMed | CAS Yantai Institute of Coastal Zone Research and Shandong Marine Resource and Environment Research Institute
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2015

C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other C-type lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca(2+)-binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different up-regulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca(2+)-dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.


PubMed | CAS Yantai Institute of Coastal Zone Research and Shandong Marine Resource and Environment Research Institute
Type: | Journal: The Science of the total environment | Year: 2016

A verified method for measuring Semicarbazide (SEM) in seawater, sediments, and shellfish was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 30 stations were radially distributed in Jincheng and Sishili Bays in the Bohai and Yellow Seas, and 1025 monitoring data were collected in 41 voyages, 615 seawater samples, 320 sediment samples and 90 shellfish samples. The concentration ranged from 0.011g/L to 0.093g/L and 0 to 0.75g/kg in seawater and shellfish respectively, but SEM in sediment was all below the limit of detection. Temporal and spatial distribution of SEM was investigated using multivariate analysis to estimate the degree of SEM pollution. Based on the SEM concentration in the three sample types, together with our previous findings, early warning values were deduced for SEM in seawater, and the developed method overcame shortcomings with existing technologies. The results may be helpful to draft national baseline values for SEM in seawater and sediments, and provide a scientific basis for assessing the impacts of SEM on marine ecology and human health.

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